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DNA Continued (Deoxyribonucleic Acid) Genetic material of cells… • GENES – units of genetic material that CODES FOR A SPECIFIC TRAIT • Like for eye color or hair color. • Genes located on chromosomes • DNA is made up of repeating molecules called NUCLEOTIDES Genetic Diversity… • Different arrangements of NUCLEOTIDES in a nucleic acid (DNA) provides the key to DIVERSITY among living organisms. The Code of Life… • The “code” of the chromosome is the SPECIFIC ORDER that bases in the nucleotides occur. A T C G T A T G C G G… See p. 332 DNA is wrapped tightly around histones and coiled tightly to form chromosomes DNA Replication • During Interphase • DNA must be copied • The DNA molecule produces 2 IDENTICAL new complementary strands following the rules of base pairing: A-T, G-C DNA Replication • Each strand of the original DNA serves as a template for the new strand • See page 334 DNA Replication The two strands of the parental molecule separate, and each functions as a template for the making of a new complementary strand. . DNA Template Parental DNA New DNA Steps in Replication 1. An enzyme called DNA Helicase unwinds and unzips the DNA molecule Steps in Replication 2. An enzyme called DNA Polymerase attaches to the parent DNA molecule in several different spots. It inserts the complementary bases, making two copies of the DNA molecule Speed of Replication Prokaryotes have a single circular strand of DNA to replicate. This takes about 40 minutes. Speed of Replication Eukaryotes if their DNA was done by one polymerase molecule per chromosome would take about a month for the DNA to replicate. Multiple polymerase latch on the replicating DNA simultaneously and as a result replication in humans takes about an hour. Replication Quiz A---? G---? C---? 2. When does replication occur? T---? 3. Describe how replication works. A---? G---? A---? 4. Use the complementary rule to G---? create the complementary C---? strand: A---? G---? T---? 1. Why is replication necessary? Replication Quiz A---T 1. Why is replication necessary? G---C So both new cells will have the correct C---G DNA T---A A---T 2. When does replication occur? G---C During interphase (S phase). A---T G---C 3. Describe how replication works. C---G Enzymes unzip DNA and complementary A---T nucleotides join each original strand. G---C 4. Use the complementary rule to T---A create the complementary strand: What is the purpose of DNA? • DNA controls cell function by serving as a template (blueprint) for PROTEIN structure. (Remember Proteins made of amino acids.) What do proteins do? • Proteins are found everywhere .... every nook and corner in the cell .. most things are made from proteins as well The processes to make proteins We need RNA • Single stranded nucleic acid • Sugar in the nucleotide is RIBOSE not deoxyribose • Uracil replaces Thymine in the base pairs. Types of RNA Messenger RNA mRNA Carries code from DNA to ribosomes to make proteins Ribosomal RNA Makes up the ribosome rRNA Transfer RNA “grabs” the amino acids from the cytoplasm to make proteins tRNA Transcription • DNA can “unzip” itself and RNA nucleotides match up to the DNA strand. mRNA is produced by a process known as : Transcription Making proteins Anticodon: three complementary nucleotides on tRNA Codon: (Three nucleotides on mRNA) How are Proteins made? • By reading the codon (3 nucleotides in a row) on the mRNA by the ribosome RNA. • Then matching the codon with the anticondon (3 opposite nucleotides) on the tRNA • The tRNA is carrying an amino acid which then links together with other amino acids. • Called Translation Translation • The cell uses information from “messenger” RNA to produce proteins 1. 2. 3. 4. 5. 6. Transcription/Translation Quiz Why is transcription necessary? Describe transcription. Why is translation necessary? Describe translation. What are the main differences between DNA and RNA. Using the chart on page 338, identify the amino acids coded for by these codons: UGGCAGUGC 1. Why is transcription necessary? Transcription makes messenger RNA (MRNA) to carry the code for proteins out of the nucleus to the ribosomes in the cytoplasm. 2. Describe transcription. RNA polymerase binds to DNA, separates the strands, then uses one strand as a template to assemble MRNA. 3. Why is translation necessary? Translation assures that the right amino acids are joined together by peptides to form the correct protein. 4. Describe translation. The cell uses information from MRNA to produce proteins. 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC tryptophan-glutamine-cysteine AMAZING DNA FACTS… • DNA from a single human cell extends in a single thread for almost 2 meters long!!! • It contains information equal to some 600,000 printed pages of 500 words each!!! (a library of about 1,000 books)