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A View of the Cell Chapter 7 The Discovery of Cells The Plasma Membrane Eukaryotic Cell Structure Web links to study guides Cell Theory All living things are made up of 1 or more cells. A cell is the smallest unit that can carry on all of the processes of life Before the 17th century, no one knew that cells existed. One of the 1st microscopes was made by the Dutch drapery store owner Anton von Leewenhoek. 1st person to observe & describe microscopic organisms & living cells Animalcules Robert Hooke 1665 – Robert Hooke used a microscope to examine a thin slice of cork & described what he saw as “a great many little boxes”. He thought the boxes looked like small rooms in which monks lived, so he called them “Cells” Hooke’s microscope Micrographia Cell Theory 1838 – German botanist Matthies Schleiden concluded all plants are composed of cells. 1839 – German zoologist Theodor Schwann concluded that animals are made up of cells. 1855 – German physician Rudolf Virchow concluded that plant cells come only from a plant and animal cells arise only from an animal Cells only come from other cells! Cell Theory – three main parts All living organisms are composed of one or more cells. Cells are the basic unit of structure & function in an organism. Cells come only from reproduction of existing cells. This means that all cells came from ONE original. Light Microscopes Light microscopes can magnify up to 1,500X and resolve objects as small as 200 nanometers (2x10-7) Electron Microscopes 1st built by Ernst Ruska (1986 Nobel Prize) in 1933. He used accelerated electrons & magnetic coils to make an image. EMS can resolve objects that are 103 time smaller than the light microscope. Transmission EMS can magnify a sample up to 50,000x and provide a resolution of 0.1 x 10-9 m. Prokaryote - Eukaryote Stuff Cells are made of Intro Clip Plasma Membrane Like a “smart” security blanket for the cell. Lets some molecules in and keeps others out Plasma Membrane Lipid bilayers have 2 important characteristics 1. Polar molecules can stick together like magnets, but are not PERMANENTLY stuck like in a chemical bond. 2. Lipid bilayers are fluid. (imagine life preservers floating on a pool with strings hanging down into the water) What is a mosaic? The patterns that the proteins make on the membrane make the surface look like a mosaic. Phospholipid membrane Basic Building Block of the cell membrane is the phospholipid. Double layer of lipids forms a flexible lipid bilayer. (bi = 2) Try this! Passage Ways Proteins in the cell membrane serve many purposes. Channels – Proteins act as passage ways through which only certain molecules can pass. Receptors – Proteins transfer information into the cell by reacting to certain other molecules Passage Ways Markers – Proteins are the “name tags” of cells giving them identity. Cellular Boundaries The plasma membrane Acts as a selectively permeable membrane Is flexible The cell wall Plants, fungi, most bacteria and some protists Fairly rigid Gives additional support and protection Plant Cell Wall Made up of carbohydrate Cellulose Forms a thick mesh Functions to protect the cell and give it support ONLY Very porous – allows molecules to pass through DOES NOT select what materials can enter the cell – that is the cell membrane’s job Control in the cell Start with the Nucleus 1. Nucleus – leader of the eukaryotic cell. Contains the directions to make proteins in chromatin – strands of DNA Remember, the types of proteins a cell produces are what makes it unique Proteins are necessary for ALL functions of the cell We call this DNA chromosomes when it is all coiled up before the cell reproduces itself THIS is where all heredity information is stored Cell Nucleus Nucleus cont. 2. Nucleolus - inside the nucleus Assembles ribosomes that will move outside the nucleus into the cytoplasm (more on ribosomes later, but they are the factories that make proteins from amino acids) 3. Nuclear Envelope – pair of membranes that surround DNA & nucleolus 4. Nuclear Pores – allows material to pass through nuclear envelope 5. Cytoplasm – fluid region between cell membrane and nucleus Cytoplasm 5. Cytoplasm surrounds the nucleus, suspends the organelles Has two main components – Cytosol & organelles Cytosol – consists mostly of water along with proteins, carbohydrates, salts, minerals, & organic molecules. Organelles (Endoplasmic reticulum, mitochondria etc.) carry out specific functions in the cell Ribosomes 6. Ribosomes - manufacturing centers A. This is where proteins are made. B. Free ribosomes – scattered throughout the cytoplasm. Produces proteins used in the cell. C. Fixed ribosomes – produce proteins used outside the cell Endoplasmic Reticulum the chemical reaction center 7. Endoplasmic Reticulum – a large workspace within the cell Complex system of membranes that forms a transport system between the nucleus and the cytoplasm May have ribosomes attached – Rough ER. No ribosomes – Smooth ER Smooth Endoplasmic Reticulum 7a. Smooth ER A. Builds lipids for cell membrane B. neutralizes poisons (will break down morphine & amphetamines) Rough Endoplasmic Reticulum 7b. Rough ER A. Ribosomes attached B. sites for proteins that are exported Golgi Bodies 8. Molecular mail of the cell A. saclike pieces of membrane pinched off that are full of chemicals Ex – found in cells that produce large number of chemicals – saliva & digestion aids Mitochondria 9. Power house A. Releases energy from the nutrients taken in B. Ex – Found in greater numbers in liver cells & heart cells Lysosome 10. Lysosome recycling centers of the cell A. Contain strong destructive enzymes that break down food & foreign objects Ex – Found in white blood cells Stuff for Lysosomes to destroy… Cytoskeleton 11. Maintain the shape of cell & hold organelles in place Microtubules- hollow tubes like plumbing pipes Microfilaments- have a structure that resembles rope and can contract causing movement. Muscle cells have many microfilaments Centrioles 12. Centrioles organize the microtubules of the cytoskeleton during cell division in animal cells Plant cells don’t have centrioles Cilia & Flagella 13. Cilia & Flagella - Hairlike organelles that extend from the surface of the cell, where they assist in movement Cilia- short hairlike projections Flagella- long whiplike projections In humans beating cilia line parts of the respiratory system, moving dust particles & bacteria away from the lungs Vacuoles 14. Vacuoles are membrane-bound spaces in the cell used for storage of materials. Examples of things the vacuole may store are food, enzymes and waste. Vacuoles are difficult to see in animal cells, since they are so small. Plant vs. Animal Cells 1. Both have the same organelles. 2. Both are Eukaryotic cells. Plants have 1. Large vacuoles – may take up to 90% of the volume of a cell A. gives support to the cell B. stores food or waste material Animal cell Usually smaller in size Cell wall is absent. Cellulose in any form is absent Cytoplasm is denser, more granular and occupies most of the space in the cell . Vacuoles absent. If present, they are small, temporary and concerned with excretion or secretion Vacuoles are large and prominent. May be one or more Plastids are absent Plastids are usually present Centrosome is present Centrosome is absent. Instead two small clear areas called polar caps are present Prominent and highly complex Golgi bodies present near nucleus Contain several sub units of Golgi apparatus called dictyosomes Plant cell Usually larger in size Cell wall made up of cellulose is present Cytoplasm is pushed to the periphery and forms a thin lining against the cell wallThe central space in the cell may be occupied by a large, single vacuole Plant Cells 2. Chloroplasts – contains chlorophyll (gives the plant leaves their green color) A. site of photosynthesis Plant Cells 3. Chromoplasts – color pigments found in flower petals (red, orange, and yellow) A. no known chemical function in the cell B. attracts animals & insects to the flower Plant Cells 4. Leucoplasts – colorless vacuoles A. storage for starch and fat molecules 5. Cell Wall – thick outer layer that protects & supports the cell. A. made of cellulose