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Cell Structure and function Chapter 4 (in the Rizzo Class Sequence) Chapter 7 in Prentice Hall Section Outline Section 7-1 • 7–1 Life Is Cellular A. The Discovery of the Cell 1. Early Microscopes 2. The Cell Theory B. Exploring the Cell C. Prokaryotes and Eukaryotes 1. Prokaryotes 2. Eukaryotes Watch Out!!!! • Keep a watchful eye out for website links some can be used to access labs, animations or videos hosted online. Anton van Leeuwenhoek, Section Outline 1665 - 75 Anton van Leeuwenhoek, • the person incorrectly given credit for the invention of the microscope • studies organisms living in pond water . • He calls them "Animalcules." Section Outline Robert Hooke • • 1665 - looks at cork under a microscope. Calls the chambers he see "cells" Section Outline Schleiden and Schawann 1830 - German scientists summarize the findings of many scientists and conclude that all living organisms are made of cells . Section Outline Rudolf Virchow 1855- Stated, ”All Cells come from Pre-existing Cells” Cell Theory Section Outline “All organisms are composed of cells” 1. The cell is the structural unit of life - units smaller than cells are not alive 2. The cell is the Functional unit of life 3. Cells arise by division of preexisting cells - spontaneous generation does not exist Section Outline Cell Theory 3 facts 3 Exceptions Bacterial Forms Binary Fission Prokaryotic and Eukaryotic Cells Prokaryotic vs Eukaryotic Cell membrane Cytoplasm Prokaryotic Cell Cell membrane Cytoplasm Nucleus Eukaryotic Cell Organelles Prokaryotic and Eukaryotic Cells Section 7-1 Cell membrane Prokaryotes vs Eukaryotes Cytoplasm Prokaryotic Cell Cell membrane Cytoplasm Nucleus Eukaryotic Cell Organelles Venn Diagrams Prokaryotes *simpler **Normally single celled Bacteria ***NO (lacks) membrane organelles Eukaryotes Cell membrane Contain DNA Cytoplasm Ribosomes *Complex **Forms tissues ***Has Membrane bound Organelles EX. Nucleus Endoplasmic reticulum Golgi apparatus Lysosomes Vacuoles Mitochondria Cytoskeleton Interest Grabber Section 7-2 Division of Labor • A cell is made up of many parts with different functions that work together. Similarly, the parts of a computer work together to carry out different functions. • Working with a partner, answer the following questions. • 1. What are some of the different parts of a computer? – What are the functions of these computer parts? • 2. How do the functions of these computer parts correspond to the functions of certain cell parts? Nucleus • Nuclear Double membrane with pores – Outer membrane continuous with ER • Chromatin: Single stranded, balled up chromosomes • Chromosomes - protein and DNA complexes • Nucleolus - involved in the synthesis and assembly of Ribosomes Ribosomes • The "factories" of the cell involved in protein synthesis • May either be free or bound to the ER Endoplasmic Reticulum an extensive membranous network continuous with the outer nuclear membrane • Smooth ER: lacks Ribosomes and is involved in membrane lipid synthesis • • Rough ER: has Ribosomes and is involved in secreted protein synthesis Intracellular circulation Golgi Who? apparatus, complex, bodies • Flattened vesicles in stacks which receive protein from ER • Form secretory vesicles to transport proteins to different parts of the cell – (vacuole, Lysosomes, etc) or for secretion Animal Vacuole • major storage for food, water enzymes and waste • Digestive - break down of macromolecules • Storage - ions, sugars, amino acids, toxic waste • Ex. Contractile vacuole, food Lysosomes • found mostly in animal cells • contain enzymes for use in the hydrolysis (breakdown) of macromolecules (digestion) – Breaks down • food • damaged or Old cell organelles – Suicide Bags?? – Sun BURN!!!! – Ex. Peroxisome Lysosomes Peroxisome • Eukaryotic organelle that degrades fatty acids and amino acids • Also degrades the resulting hydrogen peroxide Mitochondria • Found in ALL eukaryotic cells (yes, even in plant cells) • Site of aerobic respiration – sugars + O2 - - > ATP + CO2 + H2O • Contain DNA which codes for mitochondrial proteins, Ribosomes, etc. • Divide by a process similar to binary fission when cell divides (asexual replication??) • Enclosed in a double membrane system Mitochondria Check your answers: Plant Cell Ribosome (free) Smooth endoplasmic reticulum Vacuole Cytoplasm Chloroplast Ribosome (attached) Cell Membrane Nuclear envelope Cell wall Nucleolus Golgi apparatus Nucleus Mitochondrion Rough endoplasmic reticulum Plant Cell Check your answers: Animal Cell Ribosome (attached) Nucleolus Nucleus Cell Membrane Nuclear envelope Mitochondrion Smooth endoplasmic reticulum Rough endoplasmic reticulum Centrioles Golgi apparatus Vacuole Ribosome (free) Centrosomes and Centrioles 1. 2. 3. 4. Found only in Animal Cells The centrosome, also called the "microtubule organizing center", is an area in the cell where microtubles are produced. Within an animal cell centrosome there is a pair of small organelles, the Centrioles, each made up of a ring of nine groups of microtubules. There are three fused microtubules in each group. The two Centrioles are arranged such that one is perpendicular to the other. Chloroplasts 1. Found only in plant cells 2. Site of photosynthesis – conversion of solar energy to chemical energy in the form of ATP and sugars 3. Contain DNA which codes for chloroplast proteins, Ribosomes, etc. 4. Divide when plant cell divides 5. Enclosed in a double membrane envelope that does not envelope into the chloroplast Chloroplasts • Thylakoid is a third internal membrane system – contains membrane-bound photosynthetic pigments – site of photochemistry (the conversion of light energy to ATP) – site of O2 generation • Stroma is soluble portion of chloroplast – site of CO2 fixation – site of sugar synthesis (carbon metabolism) – site of chloroplast protein synthesis???? Chloroplasts Chloroplasts The photosynthesis reactions can be broken down into two components: 1. The light-dependent reactions (the "light" reactions) - occur on the thylakoid membranes 2. The light-independent reactions (the "dark" reactions) - occur in the stroma Chloroplasts Cytoskeleton Microfilaments – solid protein (actin) which is assembled at one end and disassembled at the other end Intermediate filaments - rope-like fibrous proteins – provide structural reinforcement – anchor organelles – keep nucleus in place Microtubules - hollow tubes – maintains cell shape – anchor organelles – movement of organelles – track for motor proteins Cell Movements 1. internal, referred to as cytoplasmic streaming AKA CYCLOSIS Internal movements of organelles are governed by actin filaments. These filaments make an area in which organelles such as chloroplasts can move. 2. external, referred to as motility. (Motile) Determined by special organelles for locomotion. • Pseudpods • Cilia, 1000s of hairs, being much shorter • Flagella, 1-12 hairs They both have the characteristic 9 + 2 arrangement of microtubules. Complete the Venn Diagram on page 9 In Your “CELL PROTFOLIO” packet. Animal Cells __________ Many more __________ Plant Cells 1__________ 2__________ 3 __________ 4 __________ 5 __________ 6 __________ 7 __________ 8 __________ 9 __________ __________ __________ Large __________ Venn Diagrams Animal Cells Plant Cells 1 Cell membrane 2 Ribosomes Cell Wall 3 Nucleus Chloroplasts 4 Endoplasmic reticulum Large Water Vacuole Many more 5 Golgi apparatus Vacuoles 6 Lysosomes Mitochondria 7 Vacuoles 8 Mitochondria The cell appears The cell appears To have more straight Irregular shaped 9 Cytoskeleton Edged “walls” Centrioles 7–3 Cell Boundaries Section Outline A. Cell Membrane B. Cell Walls C. Diffusion Through Cell Boundaries Measuring Concentration (ppm) 1. Passive Transport a. Diffusion b. Osmosis Osmotic Pressure c. Plasmolysis d. Facilitated Diffusion 2. Active Transport Molecular Transport a. Endocytosis Phagocytosis Pinocytosis b. Exocytosis Section Outline Cell Wall Provides support and protection Found in: Fungi-chitin Algae-composed of cellulose Plants-composed of cellulose Cellulose (plant starch) is probably the single most abundant organic molecule in the biosphere. Tough carbohydrate fibers It is the major structural material of plants Wood = Paper Corn Kernels Lettuce (mostly cellulose) Cell Membrane • Regulates what enters and leaves the cell • Made up of proteins, lipids and carbohydrate markers. • Also known as : – “The Gate Keeper” – lipid bilayer or Bilipid Layer – plasma membrane – Selectively Permeable barrier Lipid Bilayer to the Fluid Mosaic • A model used to conceptualize the cells plasma membrane • the membranes are described as a structurally and functionally asymmetric lipid-bilayer studded with embedded proteins • Named so because the phosopholipids shift position in the membrane almost effortlessly (fluid), • (A mosaic is an image made up of many small images). – Examples: lipid molecules, various proteins, carbohydrates, and cholesterol http://home.earthlink.net/~shalpine/anim/Life/memb.htm Figure 7-12 The Structure of the Cell Membrane Section 7-3 Outside of cell Proteins Carbohydrate chains Cell membrane Inside of cell (cytoplasm) Protein channel Lipid bilayer Lipid Bilayer to the Fluid Mosaic Lipid Bilayer to the Fluid Mosaic Concentration Gradient • The difference in concentration between a region of high concentration and a region of low concentration Measuring Concentration (ppm) Mass of the solute in a given volume of solution. 12g/3L = 4g/L Parts Per _?__ Concentration Gradient • The graduated difference in concentration of a solute per unit distance through a solution. • high concentration to low concentration • HCLC Transport • Is the absorption and circulation within an organism Absorption • The passage of materials across the cell membrane and into the cell Circulation • The movement of materials within a cell and/or throughout an organism a) Intercellular circulation *blood circulation b) intracellular circulation - within the cell (cyclosis) Passive Transport vs. Active Transport Passive Transport: • Mvmt of materials Without use of energy. Active Transport • Mvmt of materials using of energy. • With Concentration Gradient • HCLC • Against Concentration gradient • LCHC • Examples: • Examples: – – – – Diffusion Osmosis Facilitated Diffusion Plasmolysis – Endocytosis • Phagocytosis • Pinocytosis – Exocytosis • Re: Contractile Vacuole Do Now Using you knowledge of transport complete the concept web Transport Passive Transport • A type of transport in which materials move across the cell membrane without using energy • With the concentration Gradient ( high concentration to low concentration) Passive transport Diffusion • The movement of particles from an area of high concentration to an area of low concentration Osmosis • The diffusion of water across a semipermeable membrane from a region of high concentration to a region of low concentration • Example of Passive transport Section 7-3 Osmosis Osmosis • Movie Effects of Osmosis on Cells • Isotonic: concentration of solutes is the same inside and outside the cell • Hypertonic: Solution has a higher solute concentration than the cell • Hypotonic: Solution has a lower solute concentration than the cell Osmosis video site http://www.ccr.buffalo.edu/etomica/app/modules/sites/Osmosis/Background1.html Facilitated Diffusion • Movie Facilitated Diffusion Glucose molecules High Concentration Cell Membrane Low Concentration Protein channel Plasmolysis •Plasmolysis is the loss of water from the cell by osmosis. •This can be proven by inducing the evacuation of water from the water vacuole by changing the environmental concentrations…. How? Plasmolysis video site red blood cells video site Plasmolysis Plasmolysis video site http://www.uic.edu/classes/bios/bios100/lectf03am/plasmolysis.jpg Plasmolysis Plasmolysis lab Active Transport • A type of transport requiring energy to move materials across the cell membrane (low concentration to high concentration) http://www.biology4kids.com/files/cell2_activetran.html Active Transport Figure 7-19 Active Transport Section 7-3 Molecule to be carried Energy Molecule being carried Active Transport Sodium-Potassium Pump Section 7-3 sodium Potassium pump demo site video Section 7-3 Ultimate in Cell Communication Nerve Cells talking to each other Phagocytosis (Click on me) Phagocytosis Online Phagocytosis (Movie) Exocytosis Video 4 Active Transport • video segment. “That's All Folks”