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1 I. Cell Theory A. Early studies led to the development of the cell theory • It was the result of the collective work of many scientists. • 1665 Robert Hooke used the word “cell” to describe the spaces in cork • 1674 Anton Von Leeuwenhoek observed living cells and called them “animalcules” 2 I. Cell Theory (continued) • 1838 Matthias Schleiden was the first to note that plants were made of cells • 1839 Theodor Schwann concluded that animals are made of cells • 1855 Rudolf Virchow reported that all cells come from other cells Cell Theory video 3 Cell Theory All organisms are made of cells. The cell is the most basic unit of life. Structure and function All cells are produced by other cells 4 Levels of Organization in Living Things 5 CELLS ORGAN SYSTEMS TISSUES ORGANS ORGANISM 6 2 Categories of Cells 1. Prokaryotic Cells lack a nucleus and most internal structures of eukaryotic cells Pro = Before Karyotic = nucleus • All prokaryotic cells are microscopic singlecelled organisms whose DNA is suspended in the cytoplasm • Bacteria are the ONLY examples of prokaryotes. 7 2 Categories of Cells 2. Eukaryotic cells have a nucleus with DNA Eu = True Karyotic = nucleus - Have other membrane bound organelles Organelles = specialized structures that perform specific jobs “tiny organs” - There are 2 types: Animal and Plant Cells - Eukaryotic cells are found in: animals, plants, fungi and protists. Prokaryote vs eukaryote video 8 II. Cell Organelles - Animal Cell 6. Ribosomes on ER 4. Nucleolus 6. Ribosomes in cytoplasm 2. Nucleus 1. Cell Membrane 3. Nuclear Membrane 8. Mitochondria 5. Smooth ER 5. Rough ER 10. Vacuole 3. Nuclear pore 11. Centrioles 7. Golgi 12. Cytoplasm 9. Lysosome 9 II. Cell Organelles (continued) A. Cells have an internal structure • Cells are highly organized structures 1. Cytoskeleton - Cyto = cell Network of proteins and fibers that is constantly changing to meet the needs of the cell Like the support beams or infrastructure of the school 2. Cytoplasm Cyto = cell Plasm = fluid The fluid portion of the cell that fills the space between the nucleus and the cell membrane in eukaryotic cells Like the inside of the school “air” 10 II. Cell Organelles (continued) B. Several organelles are involved in making and processing protein 1. Nucleus – the brain of the cell Located at the center of the cell Contains our genetic information, Controls all of the cell’s activities Determines what proteins are made “Like the principal’s office” 2. Nuclear Envelope/Membrane: Double Membrane that surrounds the nucleus Contains nuclear pores that allow certain materials to move in and out of the nucleus “Like the walls and doors surrounding the principal’s office” 3. Nucleolus: Located inside of the nucleus Made of DNA Makes ribosomes “Like the principal” 11 4. Endoplasmic Reticulum – • interconnected network of thin folded membranes helps move materials within cell Passageways • “Like hallways and staircases” Two types It’s called rough ER when it has ribosomes on it. - Site of protein making - “Like the staircases and hallways” It’s called smooth ER when there are no ribosomes on it. - Site of lipid making and other functions - “Like the elevators” 12 5. Ribosome: Made from the nucleolus Job is to make protein Ribosomes are found in three places: 1. Nucleolus 2. Attached to the Rough Endoplasmic Reticulum. 3. Freely floating throughout the cytoplasm “The information of the school” 6. Golgi Apparatus packs, stores and ships out proteins to the cell membrane so they can be released from the cell and distributed to the body. “Like the mailroom or guidance office” 7. Vesicles • Tiny sacs that isolate and transport specific molecules out of the cell. “Like envelopes” 13 C. Other organelles have various functions 1. Mitochondria: The “Powerhouses” of the cell because they produce energy from food that was digested. This is where the process of cellular respiration is carried out. Cellular respiration is a process that breaks down food (glucose) into energy (ATP) that can be used by the cell. “Like the generators of the school” Glucose + Oxygen ATP+ Carbon dioxide + water 14 2. Lysosomes: membrane bound organelles that contain enzymes Digestive sacs – destroy damaged or worn out cell parts and get rid of bacteria. “Like the clean-up crew” 3. Vacuole: Fluid filled sac that stores water, food, ions, minerals, protein and carbohydrates. “Like kitchens” These are much larger in plant cells – central vacuole 15 Why is the vacuole larger in a plant cell? Because plants rely on rain in order to receive water. Therefore the vacuole needs to be much larger because the plant has to be able to store its water for long periods of time when there is no rain. Without water a plant can not perform photosynthesis! 16 4. Centrosome it contains the centrioles 5. Centrioles: Help with cell division in a process known as mitosis form of reproduction. Found in pairs Small cylinder shaped organelle Only found in animal cells “like the students” 17 Virtual Cell Tour Take a Virtual Cell Tour 18 Movement of Animal Cells Animal cells can move by either cilia or flagella. Both composed of microtubules. Cilia - short, hair-like projections that produce movement. Flagella – a whip-like tail used for locomotion. 19 Plant cell Vacuole Smooth endoplasmic reticulum Ribosome (free) Chloroplast Ribosome (attached) Cell Membrane Nuclear envelope Cell wall Nucleolus Golgi apparatus Nucleus Mitochondrion Rough endoplasmic reticulum 20 Plant Cell A plant cell contains every organelle of an animal cell EXCEPT the centrioles! Plant cells also have 2 additional organelles that are not found in an animal cell. What are the 2 organelles of a plant cell that are not found in an animal cell? 21 1. Chloroplasts: Found only in plant cells Are needed for photosynthesis Contain the green pigment chlorophyll Chlorophyll can trap energy from the sun and use it for photosynthesis, so plants can make their own food( glucose) through photosynthesis. Carbon dioxide + Water Glucose + Oxygen 22 2. Cell wall: Found in cells of plants, algae, fungi, and bacteria It is a thick outer covering surrounding the cell membrane Provides support and protection Allows the stem of the plant to stand upright in order to carry out photosynthesis. 23 Virtual Cell Tour Take a Virtual Cell Tour Travel through the cell video 24 3.3 Cell Membrane 3.4 Diffusion and Osmosis 3.5 Active Transport 25 3.3. Cell Membrane Cell membranes are composed of two phospholipid layers. • The cell membrane has two major functions. – forms a boundary between inside and outside of the cell – Allows materials to pass in and out of the cell Lipid bilayer (selectively permeable) cholesterol protein channel 26 Phospholipid from the cell membrane • Fluid Mosaic Model - describes the arrangement of molecules that make up a cell membrane. • Flexible membrane • Moves from side to side like a fluid • Has a variety of molecules in it like a mosaic (different textures/patterns) 27 3.3. Cell Membrane (continued) • Cell Membrane is selectively permeable • Some molecules can pass while others can not 28 Movement across a cell membrane • Passive Transport Requires no energy for molecules to move across a cell membrane. • Two types of Passive Transport 1. Diffusion 2. Osmosis 3. Facilitated Diffusion • Active Transport Requires energy in order for molecules to move across a cell membrane. • Two types of Active Transport 1. Protein Pumps 2. Endocytosis 3. Exocytosis 29 IV. Diffusion and Osmosis Movement across a cell membrane A. Diffusion: Movement of substances from an area where there are more to where there is less along a concentration gradient. - This occurs until there is a balance of substances called equilibrium - The moving substance has to be smaller than the cellular pore 30 31 Osmosis – Diffusion of water across a membrane from an area where there is more water to an area where there is less water in order to reach equilibrium. *This occurs when a solute or substance is too large to pass through the cell membrane. *Water moves to dilute the substance that is too large! B. video 32 3 types of osmotic solutions Hypertonic solution – when there is more water inside the cell, water will move out of the cell in order to dilute the higher substance. - Causes a cell to shrink Hypotonic solution – when there is more water outside the cell, water will move into the cell in order to dilute the higher substance. - Causes a cell to swell or expand Isotonic solution – when both sides of the cell membrane are at equilibrium 33 Osmosis Hypertonic - Because the solution outside the cell is greater than inside the cell – Water will leave; causing the cell to shrink Hypotonic - Because the solution outside the cell is less than inside the cell – Water will enter; causing the cell to swell Isotonic - Because the solution outside the cell is equal or the same as inside the cell – Water will move back and forth at an equal rate 34 Facilitated Diffusion Some particles can not diffuse through the membrane because of their size, or their charge They need to pass through a carrier protein Diffusion with help (High to low) Osmosis rap 35 Active transport – molecular pumps Move things AGAINST the concentration gradient: low to high Requires energy! ATP!! A transport protein PUMPS the molecule to the other side of the membrane 36 Active Transport Endocytosis – process of taking molecules into a cell by engulfing them in a membrane Exocytosis – process of releasing a substance out a cell by the fusion of a vesicle with the cell membrane. 37 Pin the Organelle on the Animal Cell 38 Pin the Organelle on the Plant Cell Smooth endoplasmic reticulum Ribosome (free) Ribosome (attached) Cell Membrane Nuclear envelope Nucleolus Golgi apparatus Nucleus Mitochondrion Rough endoplasmic reticulum 39