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Protein Synthesis: Transcription & Translation Unit 4: DNA Chapter 12-3 DNA > mRNA > ribosome > protein Learning Goals • 1. Summarize the overall process of protein synthesis including the “central dogma”. • 2. Describe Transcription in detail including where it takes places and the roles of introns and exons. • 3. Describe Translation in detail including where it takes place, codons, anti-codons, tRNA, amino acids, and protein • 4. Explain how gene expression works. Standards • Standard 1d: The central dogma of molecular biology outlines the flow of information from transcription of ribonucleic acid (RNA) in the nucleus to translation of proteins on ribosomes in the cytoplasm. •Central Dogma Rule: •DNA > RNA > ribosome > protein Protein Synthesis Overview • Two processes are required: – 1. Transcription: DNA > mRNA – 2. Translation: mRNA > protein Transcription: DNA > mRNA • Process of making messenger RNA (mRNA) from a DNA template to take the DNA info outside of the nucleus • DNA can NOT leave the nucleus (too big) • Messenger RNA (mRNA) carries the info encoded in DNA out of the nucleus to the ribosomes located in the cytoplasm. • Steps of Transcription – 1. DNA strands unwind & separate – 2. DNA strand containing a specific gene serves as a template strand – 3. RNA nucleotides are matched to complimentary DNA bases – 4. mRNA molecule is complete and DNA rewinds • mRNA Processing – Introns: the mRNA contains extra info (junk) that must be removed (cut out) – Exons: the remaining pieces carry useful information are spliced (connected) together to make the complete mRNA • The bases of mRNA pair with DNA bases but RNA replaces T with U DNA A T G C • DNA: • mRNA: RNA U A C G CTG TAC GGA ---> Transcription GAC AUG CCU template strand Now you try… • Based on the DNA template, create the matching mRNA strand: • DNA: C T A T G C A A A C T A T A G GAU ACG UUU GAU AUC • mRNA: ______________________________ Check: Are there any T’s in your mRNA? There shouldn’t be!!! Translation: mRNA > protein • Process of making proteins from info on mRNA – mRNA travels out of nucleus to the ribosome, which “reads” the mRNA as a series of 3 letter words called codons • Codon: 3-base code on mRNA that codes for a specific amino acid – Ex. CGU = alanine GUU = valine • The sequence of bases in DNA tell mRNA what order amino acids must join together to make a particular protein. Codons found in mRNA Third Base First Base Second Base • Transfer RNA (tRNA) matches up with each codon and transfers the correct amino acid • Each tRNA molecule has 3 unpaired bases called the anticodon that is complementary to one mRNA codon. • Each amino acid is added to a growing chain of amino acids • Protein = peptide bonds connecting amino acids – Many different types of proteins exist because the number and sequence of amino acids can be different Second Base First Base Practice translating mRNA into amino acids: mRNA: AUG AAA AGU UGU CUG GUU UAA A.A: Met - Lys – Ser - Cys – Leu – Val - Stop ______________________________________ Third Base Codons found in mRNA How DNA determines proteins • DNA molecules serve as templates for making messenger RNA molecules • Messenger RNA molecules move to ribosomes • Transfer RNA molecules bring amino acids to the ribosome • Polypeptides (proteins) are formed as ribosomes move along the messenger RNA strand Gene Expression • DNA in all of your body cells is the same! – Ex: DNA in your eye cells is the same DNA that is in your skin cells. • Different types of cells express different genes. – Ex: Your eyes may be green, while your skin is brown. • Specialization of cells is due to different patterns of gene expression, rather than different genes themselves. – Liver cells express different genes than blood cells • DNA: TAC TCC AGC GCA ACT ----Transcription----> • mRNA: AUG AGG UCG CGU UGA ----Translation---> • A.A.: met arg ser arg stop Learning Goals • 1. Summarize the overall process of protein synthesis including the “central dogma rule”. • 2. Describe Transcription in detail including where it takes places and the roles of introns and exons. • 3. Describe Translation in detail including where it takes place, codons, anti-codons, tRNA, amino acids, and protein • 4. Explain how gene expression works.