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Basic Cell Structure Modified by Georgia Agricultural Education Curriculum Office June, 2002 August 2008 Cells • Basic building blocks of life • Understanding of cell morphology is critical to the study of biotechnology August 2008 Cell • Smallest living unit of an organism • Grow, reproduce, use energy, adapt, respond to their environment August 2008 Cell • Many cannot be seen with the naked eye • A cell may be an entire organism or it may be one of billions of cells that make up the organism August 2008 Cells • Divided and classified in many ways • One common classification method is the presence or absence of a cell nucleus August 2008 Nucleus • Relatively large structure within a cell • Directs cell activities • Some simple single-celled organisms lack a nucleus August 2008 Nucleus • Prokaryotes – have no nucleus • Eukaryotes – have a nucleus August 2008 Prokaryotes • Single celled organism • Kingdom Monera • Lacks a cell nucleus • Internal structure is less organized than other cells August 2008 Prokaryotes • Genetic info needed is within the cell • Two most common – blue green bacteria and true bacteria August 2008 Blue green bacteria • Have ability to produce their own food like plants • Most bacteria get their food from other sources August 2008 Prokaryotes • Less complex than eukaryotes • Still have many of the basic internal characteristics August 2008 Common prokaryote structures • Cytoplasm • Chromosomal material • Cell membrane and wall • Ribosomes • Some have flagellum for mobility August 2008 Eukaryotes • Organism made up of cells that have a nucleus • May be single celled – Kingdom Protista • May have billions of cells like plant and animal kingdoms August 2008 Eukaryotes • Single celled eukaryotes include paramecia, euglena, and diatoms August 2008 Eukaryotes • Typically are highly organized • Infinite number of shapes and purposes August 2008 Eukaryotes • Human body has at least 85 different cell types • All eukaryotes have a number of structures in common August 2008 Common structures • Organelles – smaller parts of the cell with specific functions • Cell membrane – acts as a gatekeeper August 2008 Cell membrane • Controls what enters and leaves the cell • Plants, algae, and bacteria typically have a tough outer structure known as a cell wall August 2008 Cell wall • Provides additional structure and protects the cell from pressure caused by movement of water • Animal cells do not have walls August 2008 Cytoplasm • Organelles are surrounded by a jelly-like substance • Primary component is water August 2008 Nucleus • Largest and most identifiable part of the cell August 2008 Other organelles • Ribosomes • Mitochondria • Golgi bodies • Endoplasmic reticulum August 2008 Other organelles • Lysosomes • Vacuoles • Chloroplasts in plants August 2008 Nucleus • Brain of the cell • Site of much of the manipulation done in biotechnology August 2008 Nucleus • Controls activity of the cell • Round or oval structure • Typically found in the middle of the cell • Appears darker than surrounding material August 2008 Nucleus • Surrounded by a membrane that controls passage of materials in and out of the area August 2008 Nucleus • Contains DNA • DNA is located on chromosomes • Most organisms are diploid – have tow chromosomes for each trait August 2008 Diploid • Humans – 23 pair • Goldfish – 90 chromosomes, 45 pair August 2008 Cytoplasmic Organelles • Organelles are required for proper cell function • Ribosome – make proteins for cellular use and communication • May be attached to endoplasmic reticulum August 2008 Endoplasmic reticulum • Layered membraneous • Make and transport proteins August 2008 Vacuole • Large organelle, without specific shape • Store waste or raw materials used in synthesis of proteins August 2008 Golgi Bodies • Golgi apparatus • Similar to endo. Ret. • Look like a thick elastic band that has been folded several times August 2008 Golgi Bodies • Adds modifications to unfinished proteins • Makes lysosomes August 2008 Lysosome • Small and round • Store enzymes that break down food into chemical compounds • Destroy cell organelles and the cell itself August 2008 Mitochondria • Energy factories • Rod-shaped • Change food into molecules that can be used for energy August 2008 Mitochondria • Contain DNA • Can replicate themselves August 2008 Chloroplast • Found in plants and some other organisms • Contain chlorophyll August 2008 Cellular processes • Respiration • Osmosis • Diffusion • Photosynthesis August 2008 Respiration • Cells break down carbohydrates and other molecules to produce energy • Oxidation of glucose is one of most common forms August 2008 Respiration • Breaking down of sugar into water, carbon dioxide, and energy • Energy is used to do the work of the cell August 2008 Osmosis & Diffusion • Transportation of raw materials, wastes, and synthesized materials out of the cell August 2008 Diffusion • Movement of molecules; especially gases and liquids from areas of high concentration to areas of low concentration August 2008 Osmosis • Specific type of diffusion • Movement of water through a membrane from areas of high concentration to areas of low concentration August 2008 Equilibrium • Diffusion and osmosis happen to enable a cell to reach equilibrium August 2008 Cellular reproduction • Three ways: –Fission –Mitosis –Meiosis August 2008 Fission • Prokaryotes • Cell grows larger and makes a second copy of its DNA • At some point the cell membrane divides the cell by the growth of a transverse septum August 2008 Fission • Two new cells are formed as the division grows inward from either side of the cell • Two cells now called “daughter cells” • Sometimes known as “binary fission” August 2008 Mitosis • Eukaryotes • Requires 6 stages • Interphase, prophase, metaphase, anaphase, telophase, cytokinesis August 2008 Mitosis • Creates cells that are identical to the original cell. • Have the entire compliment of chromosomes existing in pairs - diploid August 2008 Meiosis • Division of sex cells • Stages of Meiosis I: Interphase I, prophase I, metaphase I, anaphase I, telophase I, cytokinesis I August 2008 Meiosis • Meiosis II • Interphase II, prophase II, metaphase II, anaphase II, telophase II, cytokinesis II August 2008 Meiosis • Each cell receives one chromosome from each pair randomly • Resulting cells have half the normal number of Chromosomes - haploid August 2008