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Basic Cell Structure Cells Basic building blocks of life Understanding of cell helps the study of biotechnology Cell Smallest living unit of an organism Grow, reproduce, use energy, adapt, respond to their environment Cell Many cannot be seen with the naked eye A cell may be an entire organism or it may be one of billions of cells that make up the organism Cells Divided and classified in many ways One common classification method is the presence or absence of a cell nucleus Nucleus Relatively large structure with a cell Directs cell activities Some simple single celled organisms lack a nucleus Nucleus Prokaryotes – have no nucleus Eukaryotes – have a nucleus Prokaryotes Single celled organism Kingdom Monera Lacks a cell nucleus Internal structure is less organized than other cells Prokaryotes Genetic info needed is within the cell Two most common – blue green bacteria and true bacteria Blue green bacteria Have ability to produce their own food like plants Most bacteria get their food from other sources Prokaryotes Less complex than eukaryotes Still have many of the basic internal characteristics Common prokaryote structures Cytoplasm Chromosomal material Cell membrane and wall Ribosomes Some have flagellum for mobility Eukaryotes Organism made up of cells that have a nucleus May be single celled – Kingdom Protista May have billions of cells like plant and animal kingdoms Eukaryotes Single celled eukaryotes include paramecia, euglena, and diatoms Eukaryotes Typically are highly organized Infinite number of shapes and purposes Eukaryotes Human body has at least 85 different cell types All eukaryotes have a number of structures in common Common structures Organelles – smaller parts of the cell with specific funtions Cell membrane – acts as a gatekeeper Cell membrane Controls what enters and leaves the cell Plants, algae and bacteria typically have a tough outer structure known as a cell wall Cell wall Provides additional structure and protects the cell from pressure caused by movement of water Animal cells do not have walls Cytoplasm Organelles are surrounded by a jelly like substance Mostly water Nucleus Largest and most identifiable part of the cell Other organelles Ribosomes Mitochondria Golgi bodies Endoplasmic reticulum Other organelles Lysosomes Vacuoles Chloroplasts in plants Nucleus Brain of the cell Site of much of the manipulation done in biotechnology Nucleus Controls activity of the cell Round or oval structure Typically found in the middle of the cell Appears darker than surrounding material Nucleus Surrounded by a membrane that controls passage of materials in and out of the area Nucleus Contains DNA DNA is located on chromosomes Most organisms are diploid – have tow chromosomes for each trait Diploid Humans – 23 pair Goldfish – 90 chrms, 45 pair Cytoplasmic Organelles Organelles are required for proper cell function Ribosome – make proteins for cellular use and communication May be attached to endoplasmic reticulum Endoplasmic reticulum Layered membraneous Make and transport proteins Vacuole Large organelle, without specific shape Store waste or raw materials used in synthesis of proteins Golgi Bodies Golgi apparatus Similar to endo. Ret. Look like a thick elastic band that has been folded several times Golgi Bodies Adds modifications to unfinished proteins Makes lysosomes Lysosome Small round Store enzymes that break down food into chemical compounds Destroy cell organelles and the cell itself Mitochondria Energy factories Rod shaped Change food into molecules that can be used for energy Mitochondria Contain DNA Can replicate themselves Chlorplast Found in plants and some other organisms Contain chlorophyll Cellular processes Respiration Osmosis Diffusion Photosynthesis Respiration Cells break down carbohydrates and other molecules to produce energy Oxidation of glucose is one of most common forms Respiration Breaking down of sugar into water, carbon dioxide, and energy Energy is used to do the work of the cell Osmosis & Diffusion Transport of raw materials, wastes, and synthesized materials out of the cell Diffusion Movement of molecules, especially gases and liquids from areas of high concentration to areas of low concentration Osmosis Specific type of diffusion Movement of water through a membrane from areas of high concentration to areas of low concentration Equilibrium Diffusion and osmosis happen to enable a cell to reach equilibrium Cellular reproduction Three ways Fission Mitosis Meiosis Fission Prokaryotes Cell grows larger and makes a second copy of its DNA At some point the cell membrane divides the cell by the growth of a transverse septum Fission Two new cells are formed as the division grows inward from either side of the cell Two cells now called “daughter cells” Sometimes known as “binary fission” Mitosis Eukaryotes Requires 6 stages Interphase, prophase, metaphase, anaphase, telophase, cytokinesis Mitosis Creates cells that are identical to the original cell. Have the entire compliment of chromosomes existing in pairs - diploid Meiosis Division of sex cells Stages of Meiosis I: Interphase I, prophase I, metaphase I, anaphase I, telophase I, cytokinesis I Meiosis Meiosis II Interphase II, prophase II, metaphase II, anaphase II, telophase II, cytokinesis II Meiosis Each cell receives one chromosome from each pair randomly Resulting cells have half the normal number of Chromosomes - haploid