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RECAP BIOLOGICAL FACTS Competency: describe the structure and function of major and subcellular organelles STEM_BIO11/1 2-Ia-c-2 Activity: Watcha Video Time 1. View the video on: https://www.youtube.com/watch?v=URUJD5NEXC8 2. Take note of the answers of the questions: 2.1 What are the parts of the cell and their functions? Plants are different from animals: 1. plants have plastid 2. plants have cell wall Plant Cell Parts: Cell Wall Nucleus Cytoplasm Animal Cell Parts: Cell membrane Nucleus Cytoplasm Cell Wall – skeletal framework of the cell composed of cellulose – they are microfibrils embedded in gel like matrix layers: 1. Middle lamella – composed of pectin,pectose and Ca pectate 2.Primary Wall – very thin 3.Secondary Wall – thick and firm made up of lignin and suberin Aside from cellulose, there are other substances impregnated in the cell walls: Lignin – complex sugar derivatives responsible for hardening of the wood. Cutin – oily/fatty substance which makes the cell wall impermeable to water. Suberin – waxy substance rendering impermeability to water. Pectin – jelly-like substance cementing the cell wall together. Cell Membrane – Plasma Membrane, Plasmallemma, - fragile, transparent barrier, limiting membrane that contains the cell organelles and separate from outside environment. It has a core of two lipid layer in which protein molecules. Specializations: 1. Microvilli – little shaggy hairs – tiny finger like projections that greatly increase cells surface area from absorption. 2. Membrane Junctions: a. Tight Junctions – zipper like b. Desmosomes - anchoring junction , button like c. Gap junction – cylinder like B. Nucleus – small, spherical body within the cytoplasm controlling most activity. Subparts: 1. Nuclear Membrane – double membrane possessing pores surrounding the nucleus. it separates the cytoplasm from nucleoplasm also called the nuclear envelope 2. Nucleoplasm – or karyolymph, nuclear sap or karyoplasms semifluid within the nuclear membrane – act as intermediate between nucleus and cytoplasm. 3. Nucleolus –one or more spherical bodies of genetic material.- site for ribosome assembly. 4. Chromatin Network – Nuclear net – play role in synthesis of RNA and cell division. Contains: histone, complex proteins, RNA and DNA C. Cytoplasm – cellular material outside the nucleus and inside the plasma membrane, - site of most cellular activities – factory area of activities 3 Elements: 1. Cytosol – semi transparent fluid that suspends other elements. 2. Organelles – Metabollic machinery 3. Non-Living Inclusions – Product of metabolic processes that serves as chief reserved activities. Examples: fat droplets, crystals, secretory granules, starch grains, protein bodies. SubCellular Parts: 1. Plastids – organized globular bodies capable of self replication Types: a. chloroplastid – site of chlorophyll production Areas: 1. grana – green 2. stroma – non-green b. chromoplastid – multi-colored plastid with carotenoid pigment. c. leucoplastid – colorless plastid, serve as storage center for reserved food. Types: 1. amyloplast – store starch 2. elaioplast – store lipids 2. Mitochondria – spherical sausage-shaped bodies- inner membrane resemble a plate-like structure called cristae. –Mitochondria is also known as bio blast. Functions: a. power house of cell b. site for respiration. 3. Ribosomes – sublight, microscopic ribonucleo protein particle – tiny localized swelling. Function: actual site for protein synthesis. 4. Endoplasmic Reticulum - consist of pair of membrane separated by a gap and consist of fine canals. – function: provides network of channels for carrying substances. Types: a. Rough ER – called Granular ER – for protein synthesis b. Smooth ER – called Agranular ER – for lipid synthesis 5. Golgi Apparatus – also called golgi bodies or dictyosomes. – stocks of plate like membrane containing sacs. – known as traffic director for cellular proteins. – function – play role in cell division. 6. Microtubles – known as centrioles – long straight cylinders – function: movement during cell division. Two projections: cilia and flagella 7. Microbodies- organelles same size of mitochondria containing oxidative enzymes. Types: 1. Peroxisomes –membrane sacs with oxidative enzymes that uses molecular oxygen to detoxify a number of harmful substances including alcohol and formaldehyde. Function: disarm dangerous free radicals Free radicals – are highly reactive chemicals that scramble the structure of proteins and nucleic acid. 2. Lysosome - act as scavenger of the cell or also known as the suicide bag. 3. Glyoxisomes - isolated in seeds. 4. Spherosomes – Spherical bodies for lipid storage. 8. Microfilaments – also known as cytoskeleton – elaborate network of protein structure that extends throughout the cytoplasm. Functions: act as cell bones and muscles that provides support and shape. – also responsible for the movement of organelles. 9. Vacuole- cytoplasmic space for cell enlargement and storage center. 10. Tonoplast – or also known as vacuolar membrane - membrane surrounding the vacuole. Application: What parts of Cell has the following functions: 1. scavenger of the cell 2. traffic director of the cell 3. knows as the Bio-Blast 4. network for carrying substances 5. Site for ribosome assembly 6. responsible for protein synthesis 7. Cell enlargement 8. hair-like structure for movement 9. colorless plastid for food storage 10. fight against free radicals (10 mins) Assign members to bring the following next meeting: Bring the following next meeting 1. Onion Bulb 2. Mayana leaf or gumamela flower 3. Canal water (put in vial) 4. Disposable gloves 2 per (group) 5. scalpel 6. dropper 7. Study lamp 8. Extension wire 9. Lab gown 10. Pricker (blood) 11. glass slides and cover glass (c/o lab) 12. Microscope (c/o lab) 13. Prepared slides (c/o teacher) NOTE: Incomplete group will not be allowed to perform the experiment