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Download P310 Trypanosoma brucei PUF RNA binding proteins Katelyn Fenn
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Transcript
P310 Trypanosoma brucei PUF RNA binding proteins Katelyn Fenn and Keith Matthews Centre for Immunology, Infection and Evolution, University of Edinburgh The transmission of African trypanosomes requires a differentiation from bloodstream form parasites to the tsetse-fly midgut form, known as the procyclic form. Gene expression in African trypanosomes is largely regulated post-transcriptionally, due to the unregulated polycistronic transcription of most genes. RNA stability and turnover therefore play a major role in gene regulation, with RNA binding proteins proving to be very important in these processes. The mechanic actions of the large number of RNA binding proteins found in the T. brucei genome remain largely unknown. One of the major cellular changes upon differentiation to the procyclic form is the activation of mitochondrial genes. These genes include components of the cytochrome oxidase (COX) complex of the trypanosome respiratory chain, which is developmentally regulated. Previous studies revealed that these genes were regulated via signals in the mRNA 3’ untranslated regions (UTRs). In yeast, COX gene expression is regulated by a PUF RNA binding protein. PUF proteins are present in most eukaryotic organisms and have been shown to play a major role in both RNA stability and translational regulation. Bioinformatic analysis of the T. brucei genome revealed there are eleven PUF proteins present. To investigate if these proteins are involved in COX gene regulation, a CAT-COX UTR fusion reporter system was used. Of the three divergent TbPUF proteins tested, it appears none of these proteins are involved in COX gene regulation. Their possible role in other aspects of differentiation is discussed.
