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Student Name: ______________________
Teacher:
______________________
District:
Robeson
Assessment:
9_12 Agriculture AU71 - Biotech and Agrisci Rsch I Test 3
Description:
Unit G - DNA Extraction and Analysis
Form:
501
Date: ___________
1. What biotechnology tool can a production agriculturalist use to test the paternity of an animal conceived through a
field/pen breeding scenario with multiple males?
A.
B.
C.
D.
DNA analysis
Gene therapy
RNA extraction
Selective breeding
2. Gel electrophoresis is a simple method of DNA analysis that:
A.
B.
C.
D.
Displays the base pair sequence of specific genes.
Isolates DNA in a protein solution.
Separates DNA fragments by length on an agar gel.
Separates DNA segments by chromosome on an agar gel.
3. A technique used in forensic science, genetic manipulation, and agrimedicine to amplify a genetic sequence is:
A.
B.
C.
D.
Gel electrophoresis.
Genomic mapping.
Polymer chain reaction (PCR).
Vector insertion.
4. What animal part does not contain DNA?
A.
B.
C.
D.
Blood
Hair
Saliva
Skin
5. The use of DNA analysis in agriculture to predict the expression of negative or lethal genes in offspring prior to
birth is called:
A.
B.
C.
D.
Amniocentesis.
Forensic science.
Gene sequencing.
Umbilical diagnosis.
6. When running a DNA analysis the FIRST step is to:
A.
B.
C.
D.
Cut DNA with a restriction enzyme.
Extract DNA for analysis.
Isolate critical genes.
Take a DNA sample.
7. Kurt is extracting DNA. He is MOST likely extracting DNA from:
A.
B.
C.
D.
Almost any intact cellular tissue.
Hair clippings removed with scissors.
Inorganic materials untouched by humans.
Organisms as long as they contain blood.
8. Joey is extracting DNA in his biotechnology class. He uses detergents to:
A.
B.
C.
D.
Break down the cell membrane.
Destroy cellular proteins intertwined with DNA.
Rejoin DNA isolated in individual chromosomes.
Separate clusters of cells.
9. Cullen is extracting DNA from a strawberry. Since his goal is only to view the DNA, his FIRST step is to:
A.
B.
C.
D.
Expose the strawberry to a detergent.
Physically break apart or smash the strawberry.
Treat the strawberry with an enzyme.
Use an ethanol solution to clean the strawberry.
10. In extracting DNA from a banana, Carey uses enzymes to isolate:
A.
B.
C.
D.
Nucleic acids.
DNA and mitochondrial DNA.
Only DNA.
Only RNA.
11. Agriculturalists hoping to discover beneficial genes from a recently extinct species without a tissue sample have
the BEST chance of extracting DNA from:
A.
B.
C.
D.
Bone.
Bone marrow.
Hair.
Tooth enamel.
12. Emma uses an ethanol solution in simple DNA protocol methods to:
A.
B.
C.
D.
Break down the nuclear membrane of cells, releasing DNA.
Isolate DNA from proteins and sugars in the solution.
Protect nucleic acids from enzymes used to destroy proteins.
Sterilize the material from which DNA is to be extracted.
13. Becky slightly dilutes an ethanol solution for use in simple DNA extraction protocol in order to:
A.
B.
C.
D.
Destroy cellular and nuclear membranes, freeing DNA.
Isolate nucleic acids from other compounds in the solution.
Join DNA strands for removal from solution.
Prevent damage to nucleic acids from enzymes.
14. Rasheed is comparing the effectiveness of a simple DNA extraction protocol on both animal and plant tissues.
He uses an enzyme in the process to help:
A.
B.
C.
D.
Break down proteins mixed with nucleic acids.
Destroy simple sugars in the solution.
Preserve proteins in the solution that help nucleic acids to "clump".
Speed up the destruction of nuclear membranes.
15. Misty uses a chemical compound that in DNA protocol to break down proteins in the sample. She is MOST likely
using:
A.
B.
C.
D.
Cellulose.
Ethanol.
Fructose.
Protease.
16. A restriction enzyme is capable of removing a particular gene from its location on a:
A.
B.
C.
D.
Cell.
Chromosome.
Codon.
Nuclei.
17. Cut ends of DNA are left "sticky" so that they can be rejoined to complementary ends of other DNA fragments
by the use of:
A.
B.
C.
D.
Embryo transfer.
Hydrogen bonds.
Restriction enzymes.
Ultraviolet light.
18. Why do shorter strands of DNA group at the end of the gel during gel electrophoresis?
A.
B.
C.
D.
Electric current flows more at the far end of the gel
Shorter strands travel faster than long strands
Shorter strands travel slower than long strands
The gel is thinner at the far end
19. The use of restriction enzymes produces a solution of:
A.
B.
C.
D.
Amino acids restricted by hydrogen bonds.
Diluted enzymes.
Each allele for a given trait.
Fragmented DNA.
20. In the process of gel electrophoresis, DNA is spread by mass/size across an agar gel through the use of:
A.
B.
C.
D.
Embryo transfer.
Chemical energy.
Low voltage direct current.
Specialized proteins.
21. Gel electrophoresis chambers move DNA fragments by utilizing a:
A.
B.
C.
D.
Negative electrical charge to sort DNA fragments.
Neutral electrical charge to sort DNA fragments.
Positive electrical charge to sort DNA fragments.
Pulsating electrical charge to sort DNA fragments.
22. When preparing to conduct electrophoresis, DNA should first be treated with what substance to break the
structure apart into various gene segments?
A.
B.
C.
D.
Promoter
Restrictive agent
Restriction enzyme
Sequencer
23. When using a micropipette to introduce DNA to an electrophoresis gel, Thomas should carefully:
A.
B.
C.
D.
Inject DNA into the gel on either end.
Inject DNA into the center of the gel.
Release DNA into wells on both sides of the gel.
Release DNA into wells on one end of the gel.
24. Jim is interested in the strength of the electrical current being utilized when running gel electrophoresis. He
knows it MOST affects the:
A.
B.
C.
D.
Location of DNA fragment markers on the gel.
Length of separation between DNA fragments across the agar gel.
Speed at which DNA fragments separate across the agar gel.
Speed at which the agar gel will harden.
25. Janna is using a buffer solution as she conducts gel electrophoresis because its density helps to:
A.
B.
C.
D.
Dilute DNA fragments in solution.
Keep DNA in place in wells.
Help illuminate DNA fragment bands.
Splice entire DNA strands into gene fragments.
26. Sandy uses ethidium bromide and other bromine based indicator solutions to apply to electrophoresis gels in
order to:
A.
B.
C.
D.
Clean and sterilize the gel following the electrophoresis process.
Increase the visibility of DNA fragment bands.
Neutralize remaining restriction enzymes in the solution.
"Stick" DNA to agar gels.
27. Joy applies a buffer solution over agar gels when conducting electrophoresis in order to:
A.
B.
C.
D.
Dilute the DNA solution.
Illuminate DNA fragment bands under certain wavelengths of light.
Prevent DNA from floating out of wells in the gel.
Retain organic material in the DNA solution.