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Download Supplemental Instruction BY123 Dr. Fischer (session 19
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Supplemental Instruction BY123 Dr. Fischer (session 19) Mysupplemental.yolasite.com | [email protected] Definitions: Chromatin: Heterochromatin: Euchromatin: Histone: Nucleosome: Ligase: Nuclease: Telomere: Telomerase: Questions: The role of DNA polymerases in DNA replication is to _____. attach free nucleotides to the new strand synthesize an RNA primer to initiate DNA strand synthesis link together short strands of DNA separate the two strands of DNA all of the above One strand of a DNA molecule has the base sequence 5'-ATAGGT-3'. The complementary base sequence on the other strand of DNA will be 3'-_______-5'. TATCCA TGGATA TGGAUA UAUCCA ATAGGT DNA polymerase adds nucleotides to the _____ of the leading strands, and to the _____ of the lagging strands (Okazaki fragments). 5' end; 3' end 3' end; 5' end 5' end; 5' end 3' end; 3' end sugar group; phosphate group What enzyme joins Okazaki fragments? DNA polymerase DNA ligase topoisomerase helicase primase After the formation of a replication bubble, which of the following is the correct sequence of enzymes used for the synthesis of the lagging DNA strand? primase, helicases, DNA polymerase, ligase helicases, primase, ligase, DNA polymerase helicases, primase, DNA polymerase, ligase helicases, DNA polymerase, primase, ligase ligase, primase, DNA polymerase, helicases The removal of the RNA primer and addition of DNA nucleotides to the 3' end of Okazaki fragments in its place is carried out by _____. nuclease primase DNA polymerase I DNA polymerase III ligase Once the DNA at the replication fork is unwound by helicases, what prevents the two strands from coming back together to reform a double helix? The helicase modifies the DNA in such a way as to eliminate the affinity between the two strands. DNA polymerase follows the helicase so closely that there is no chance for the strands to come back together. Single-strand binding proteins bind the unwound DNA and prevent the double helix from reforming. The helicase pushes the two strands so far apart that they have no chance of finding each other. One of the strands is rapidly degraded, preventing the double helix from reforming.