Synthesis and Biophysical Studies of Nucleic Acid-Binding
... between an antigene oligonucleotide and its complementary sequence of doublestranded DNA, allowing the third strand antigene to bind around the duplex to form a triplex. These two hybridization mechanisms are only possible through relatively weak hydrogen bond interactions, resulting in highly varia ...
... between an antigene oligonucleotide and its complementary sequence of doublestranded DNA, allowing the third strand antigene to bind around the duplex to form a triplex. These two hybridization mechanisms are only possible through relatively weak hydrogen bond interactions, resulting in highly varia ...
PCR and qPCR product guide
... (Taq)4. This polymerase could withstand the high temperatures required during the denaturation and extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s ...
... (Taq)4. This polymerase could withstand the high temperatures required during the denaturation and extension steps of PCR, and thus fresh enzyme was not required during each cycle. Taq polymerase was commercialized in the late 1980s, spurring a boom in PCR and ultimately becoming Science magazine’s ...
Critical Factors for Successful Real-Time PCR
... oligomers, or gene-specific primers. An aliquot of the reverse-transcription reaction is then added to the real-time PCR. It is possible to choose between different types of RT primers, depending on experimental needs. Use of oligo-dT primers or random oligomers for reverse transcription means that ...
... oligomers, or gene-specific primers. An aliquot of the reverse-transcription reaction is then added to the real-time PCR. It is possible to choose between different types of RT primers, depending on experimental needs. Use of oligo-dT primers or random oligomers for reverse transcription means that ...
Peptide nucleic acids 2
... G-monomer. N2-Cbz protected guanin-9-yl acetic acid has been prepared for application in the Fmoc based synthesis of PNA. 2-Amino-6-chloropurine was alkylated with allyl bromide and following separation of the two regioisomers, the chloro group was hydrolyzed to give the guanine derivative. Subseque ...
... G-monomer. N2-Cbz protected guanin-9-yl acetic acid has been prepared for application in the Fmoc based synthesis of PNA. 2-Amino-6-chloropurine was alkylated with allyl bromide and following separation of the two regioisomers, the chloro group was hydrolyzed to give the guanine derivative. Subseque ...
Critical Factors for Successful Real-Time PCR
... Methods in real-time PCR Two-step and one-step RT-PCR For analysis of gene expression levels or viral load, the RNA first needs to be transcribed into cDNA using a reverse transcriptase. Reverse transcriptases are enzymes generally derived from RNA-containing retroviruses. RT-PCR can take place in ...
... Methods in real-time PCR Two-step and one-step RT-PCR For analysis of gene expression levels or viral load, the RNA first needs to be transcribed into cDNA using a reverse transcriptase. Reverse transcriptases are enzymes generally derived from RNA-containing retroviruses. RT-PCR can take place in ...
p68/DDX5 DEAD-box RNA helicase gene encodes a novel miRNA
... [Jensen et al. 2008]). Interestingly, these studies showed that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb ...
... [Jensen et al. 2008]). Interestingly, these studies showed that while p68 RNA helicase activity appears to be important for some functions (e.g., RNA processing), it does not appear to be required for its role as a transcriptional coactivator. The p68 gene contains a large intron (intron 11, 1.2 kb ...
Human DNA Ligase IV and the Ligase IV/XRCC4 Complex: Analysis
... NHEJ and the order in which these activities are utilized were previously surmised. Importantly, though a particular cell type will typically repair a given set of termini preferentially using one route of NHEJ, multiple NHEJ routes are often used. Moreover, each route can yield an array of products ...
... NHEJ and the order in which these activities are utilized were previously surmised. Importantly, though a particular cell type will typically repair a given set of termini preferentially using one route of NHEJ, multiple NHEJ routes are often used. Moreover, each route can yield an array of products ...
HIV-1 Reverse Transcriptase-associated
... ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none currently target the RNase H activity specifically. The work contained in this thesis de ...
... ribonuclease (RNase) H that are both absolutely necessary for viral replication and disease progression. While several drugs currently used in the clinic to treat HIV-1 target the polymerase activity of RT, none currently target the RNase H activity specifically. The work contained in this thesis de ...
SURVEY AND SUMMARY New insights into Hoogsteen base pairs
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
... which the adenine base was flipped 180◦ to form a unique set of hydrogen bonds (H-bonds) (Figure 1). Two years earlier, Rich et al. had speculated that such a pairing scheme could explain how poly(rU) associates with poly(rA)-poly(rU) duplexes to form RNA triplexes (2). These bps are now referred to ...
Studies on Polynucleotides
... In a recent paper (2), initial work on the hybridization of several synthetic deoxyribopolynucleotides belonging to the 3’ end of the tRNB sequence with the separated r strand of the +80psurrr DNA was reported. The hybridization was shown to be specific to the expected site on the tRNA gene. In the ...
... In a recent paper (2), initial work on the hybridization of several synthetic deoxyribopolynucleotides belonging to the 3’ end of the tRNB sequence with the separated r strand of the +80psurrr DNA was reported. The hybridization was shown to be specific to the expected site on the tRNA gene. In the ...
Document
... They also make enzymes The DNA controls which enzymes are made and the enzymes determine what reactions take place The structures and reactions in the cell determine what sort of a cell it is and what its function is So DNA exerts its control through the enzymes ...
... They also make enzymes The DNA controls which enzymes are made and the enzymes determine what reactions take place The structures and reactions in the cell determine what sort of a cell it is and what its function is So DNA exerts its control through the enzymes ...
Switching between polymerase and exonuclease sites in DNA
... stranded DNA). The recently solved crystal structure of the catalytic domain of Pol2 (Pol2core (aa 1–1228)) revealed that Pol ε lacks the extended -hairpin loop found in most other B-family DNA polymerases that has been proposed to interact with the template DNA and to function as a wedge by breaki ...
... stranded DNA). The recently solved crystal structure of the catalytic domain of Pol2 (Pol2core (aa 1–1228)) revealed that Pol ε lacks the extended -hairpin loop found in most other B-family DNA polymerases that has been proposed to interact with the template DNA and to function as a wedge by breaki ...
CHUKRASIA TABULARIS OXIDATIVE STRESS Research Article
... extract of different concentrations was mixed with 2.5 ml of phosphate buffer (200mM, pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50°C for 20 minutes. A volume of 2.5 ml of 10% TCA was then added to the mixture and centrifuged at 3000 rpm for 10 minutes. 2.5 ml of s ...
... extract of different concentrations was mixed with 2.5 ml of phosphate buffer (200mM, pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50°C for 20 minutes. A volume of 2.5 ml of 10% TCA was then added to the mixture and centrifuged at 3000 rpm for 10 minutes. 2.5 ml of s ...
Use of novel assays to measure in vivo base excision DNA repair
... are common ROS-induced single base alterations. Unless repaired, oxidative DNA damage results in genetic instability and tumorigenesis. The base excision DNA repair (BER) pathway is the major cellular pathway that corrects oxidation-induced single base alterations (David, O'Shea, & Kundu, 2007; Dizd ...
... are common ROS-induced single base alterations. Unless repaired, oxidative DNA damage results in genetic instability and tumorigenesis. The base excision DNA repair (BER) pathway is the major cellular pathway that corrects oxidation-induced single base alterations (David, O'Shea, & Kundu, 2007; Dizd ...
The Art of DNA Strings: Sixteen Years of DNA Coding Theory
... match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of the DNA strand forms complementary with its own other half forming the hair pin like structure. This kind of st ...
... match. But in some situation, DNA strands may not form perfect base pairing and react in undesirable manner. One situation is formation of secondary structure in which first half strand of the DNA strand forms complementary with its own other half forming the hair pin like structure. This kind of st ...
DNA PPT - Alevelsolutions
... They also make enzymes The DNA controls which enzymes are made and the enzymes determine what reactions take place The structures and reactions in the cell determine what sort of a cell it is and what its function is So DNA exerts its control through the enzymes ...
... They also make enzymes The DNA controls which enzymes are made and the enzymes determine what reactions take place The structures and reactions in the cell determine what sort of a cell it is and what its function is So DNA exerts its control through the enzymes ...
Turning h Cro into a Transcriptional Activator
... 434R(a2Cro) retain some ability to stimulate transcription; we return to this point’?n the Discussion. As controls, we tested two derivatives of 434 repressor (Koudelka et al., 1988) bearing amino acid substitutions in parts of the protein other than helix 2 and the turn between helix 2 and helix 3; ...
... 434R(a2Cro) retain some ability to stimulate transcription; we return to this point’?n the Discussion. As controls, we tested two derivatives of 434 repressor (Koudelka et al., 1988) bearing amino acid substitutions in parts of the protein other than helix 2 and the turn between helix 2 and helix 3; ...
Molecular cloning and expression of full
... BRNA was shown tobe replicated and expressed independently in cowpea protoplasts indicating that the genetic information necessary for replication of the viral RNAs and proteolytic processing of the polyproteins is located on thisRNA (Goldbach et al., 1980). Indeed the 110kD polypeptide had been sho ...
... BRNA was shown tobe replicated and expressed independently in cowpea protoplasts indicating that the genetic information necessary for replication of the viral RNAs and proteolytic processing of the polyproteins is located on thisRNA (Goldbach et al., 1980). Indeed the 110kD polypeptide had been sho ...
Current Topics LNA (Locked Nucleic Acid): High
... who incorporated three to four LNA monomers at the ends of the binding arms. Although these LNAzymes cleaved well, they were not efficient in multiple turnover, indicating that they bind their RNA target too tightly. It might be very important to carefully adjust the arm length, sequence composition ...
... who incorporated three to four LNA monomers at the ends of the binding arms. Although these LNAzymes cleaved well, they were not efficient in multiple turnover, indicating that they bind their RNA target too tightly. It might be very important to carefully adjust the arm length, sequence composition ...
CORPORATE RESPONSIBILITY AT DNA CORPORATE
... I have worked as a service advisor for consumer clients in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person ...
... I have worked as a service advisor for consumer clients in Lahti for four years now. I had not given that much thought to responsibility from the company point of view, or how wide-spread its scope is, until I participated in the training last spring. While I may not be the most ecoconscious person ...
Chapter 22: SV40 and Polyomavirus DNA Replication
... Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for review, see Fanning and Knippers 1992). Large T antigen hydrolyzes ATP, an activity that is required to act as a DNA helicase. The ATP-binding/ ...
... Deb et al. 1987). Large T antigen also binds to single-stranded DNA; this activity may be integral to its ability to unwind DNA at the replication fork (for review, see Fanning and Knippers 1992). Large T antigen hydrolyzes ATP, an activity that is required to act as a DNA helicase. The ATP-binding/ ...
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... scientists at the Rockefeller Institute in New York decided to repeat Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular ...
... scientists at the Rockefeller Institute in New York decided to repeat Griffith’s work. Led by the Canadian biologist Oswald Avery, the scientists wanted to determine which molecule in the heat-killed bacteria was most important for transformation. They reasoned that if they could find this particular ...
draft dna profiling bill 2007
... biological relationship, if any, between two individuals, living or dead without any doubt. With the objective to enhance protection of people in the society and administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The ...
... biological relationship, if any, between two individuals, living or dead without any doubt. With the objective to enhance protection of people in the society and administration of justice, analysis of DNA found at the scene of crime, of the victim or offender has been used to establish identity. The ...
Helicase
Helicases are a class of enzymes vital to all living organisms. Their main function is to unpackage an organism's genes. They are motor proteins that move directionally along a nucleic acid phosphodiester backbone, separating two annealed nucleic acid strands (i.e., DNA, RNA, or RNA-DNA hybrid) using energy derived from ATP hydrolysis. There are many helicases resulting from the great variety of processes in which strand separation must be catalyzed. Approximately 1% of eukaryotic genes code for helicases. The human genome codes for 95 non-redundant helicases: 64 RNA helicases and 31 DNA helicases. Many cellular processes, such as DNA replication, transcription, translation, recombination, DNA repair, and ribosome biogenesis involve the separation of nucleic acid strands that necessitates the use of helicases.