Download 4042 Animal and plant cell culture

1. COURSE DECRIPTION – GENERAL INFORMATION
Nataša Bauer, Maja Matulić
1.1. Course teacher
1.2. Name of the course
Animal and plant cell culture
1.3. Associate teachers
1.4. Study programme (undergraduate,
graduate, integrated)
undergraduate
obligate
1.5. Status of the course
2. COURSE DESCRIPTION
2.1. Course objectives
2.2. Enrolment requirements and required
entry competences for the course
2.3. Learning outcomes at the level of the
study programme to which the course
contributes
2.4. Expected learning outcomes at the
level of the course (4-10 learning
outcomes)
1.6. Year and semester of study
1.7. Credit value (ECTS)
1.8. Type of instruction (number of hours
L+S+E+e-learning)
1.9. Expected enrolment in the course
3rd y of undergraduate study of molecular
biology
5
1+0+2
30-40
1.10. Level of use of e-learning (1, 2, 3
level), percentage of instruction in
the course on line (20% maximum)
Introduction to basic techniques in plant and animal cell culture
cell biology
theoretical and practical knowledge of the basic techniques in plant and animal cell culture
Understanding principals of growth and development of cells and tissues
Understanding the possibilities and obstacles during cell growth and development manipulation
Ability of independent work on establishment and directioning of cell culture growth
Ability of genetic manipulation with cells and tissues
Animal cell culture
Introduction in animal cell culture: what is animal cell culture and where is it used today, comparison of cell culture with model
organisms, history of cell culture
Conditions of cell culturing: types of cell culture medium and its components, , types of substrates for cell growth,
contamination, buffers used for pH conditioning, cryopreservation t types of cell lines, growth curves
2.5. Course content broken down in detail
by weekly class schedule (syllabus)
Growth of cell colonies, methods of colony isolation, survival curves
Primary cell culture: preparation of primary culture, methods of separation of different cell types, “evolution” of the cell line
Methods of cell quantitation: cell counting, determination of the cell viability by differential staining, flow cytometry
.
Cell authentication and characterization: methods used for differentiating cells from diverse organisms, tissues and entities
1
Cell growth synchronisation: mitotic selection, synchronisation in G1 phase, inhibitors of S phase, double block in
synchronization, methods for evaluation of cell synchrony
New techniques in cell culture. Cell fusion and differentiation. Basic characteristics of cancer cell growth in culture and
senescence
Plant cell and tissue culture:
Introduction to plant cell culture: what is plant cell and tissue culture and where is it used today.
Structural, physiological and biochemical aspects of organogenesis in vitro.
Characterization of callus tissue culture and patterns of growth in tissue cultures. Establishment and use of suspension
cultures.
Establishment and manipulation of protoplasts.
Organ culture. Micropropagation and regeneration pathways and principles of vegetative propagation in vitro.
Methods used in transformation of plant cells. Regeneration of transgenic tissues and plants.
Somaclonal variation in cell cultures. Long term culturing of plant cells and cryopreservation.
Exercises
Animal cells: Introduction to cell culture laboratory and work in cell culture. Trypsinizing and counting cells. Seeding cells for
growth curve and analysis of cell growth in culture by counting. Calculating the duplication time
Primary cell culture: preparation of fibroblast culture from mouse skin. Explantation technique and preparation of cell
suspension by trypsinization
Influence of chemotherapeutics on the cell growth: growth curve obtained by counting the cells
Influence of chemotherapeutics on the cell growth: survival curve obtained by counting colonies
Cell synchronization: mitotic selection.
Plant culture:
Preparation of culture media. Sterilization of plant tissues, media and supplies.
Sterile manipulation of cells and tissues.
Selection and determination of transgenic plants.
2
Micropropagation by nodal segments.
Micropropagation by adventitious buds.
Suspension culture: determination of cell growth and viability.
Final analysis and discussion of results.
2.6. Type of instruction
x lectures
seminars and workshops
x exercises
online in entirety
mixed e-learning
field work
independent study
multimedia and the internet
x laboratory
work with the mentor
2.7. Comments:
(other)
2.8. Student responsibilities
2.9. Screening of student’s work (specify
the proportion of ECTS credits for
each activity so that the total number
of CTS credits is equal to the credit
value of the course)):
2.1. Grading and evaluation of student
work over the course of instruction
and at a final exam
Class attendance
Research
Experimental work
Report
0.2
Essay
Seminar essay
Tests
1.9
Oral exam
1
Written exam
1.9
Project
partial written exams, class attendance, reports on laboratory exercizes
Title
Allberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002): Molecular
biology of the cell, Garland Publishing, New York.
2.2. Required literature (available at the
library and via other media)
2.12. Optional literature (at the time of
the submission of the study
programme proposal)
2.13. Methods of monitoring quality that
ensure acquisition of exit
Animal cell culture exercizes, script
scientific papers from relevant journals
Practical training
(Other--describe)
(Other—describe)
(Other—describe)
Number of copies
at the library
3
Availability via
other media
x
x
x
Masters J. R. W. (2000): Animal cell culture 3. iz. Oxford University Press, Oxford
Butler M (2004): Animal cell culture & technology, 2. iz. Bios Scientific Publishers, London i New York
Freshney R. I. (2000): Culture of animal cells: A manual of basic technique. 4. iz. Willey-Lys, J. Willey & Sons. New York
partial and final written exam, oral exam, laboratory exercizes and final report
3
competences
4