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Transcript
Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability IMPORTANT INFORMATION Storage: Upon receipt of the kit, store HaeIII restriction enzyme, PTC primer/loading dye mix, and DNA marker pBR322/BstNI in a freezer (approximately â20°C). All other materials may be stored at room temperature (approximately 25°C). Use and Lab Safety: The materials supplied are for use with the method described in this kit only. Use of this kit presumes and requires prior knowledge of basic methods of gel electrophoresis and staining of DNA. Individuals should use this kit only in accordance with prudent laboratory safety precautions and under the supervision of a person familiar with such precautions. Use of this kit by unsupervised or improperly supervised individuals could result in injury. Limited License: Polymerase chain reaction (PCR) is protected by patents owned by Hoffman-La Roche, Inc. The purchase price of this product includes a limited, non-transferable license under U.S. Patents 4,683,202; 4,683,195; and 4,965,188 or their foreign counterparts, owned by Hoffmann-La Roche Inc. and F. Hoffmann-La Roche Ltd. (Roche), to use only this amount of the product to practice the Polymerase Chain Reaction (PCR) and related processes described in said patents solely for the research, educational, and training activities of the purchaser when this product is used either manually or in conjunction with an authorized thermal cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaserâs activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR process may be obtained by contacting the Director of Licensing at The Perkin-Elmer Corporation, 850 Lincoln Center Drive, Foster City, California 94404 or at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501. Printed material: The student instructions, pages 5â20, as well as the CarolinaBLU⢠staining protocol on page 27 may be photocopied as needed for use by your students. DNA KITS Learning Center Copyright © 2006, Dolan DNA Learning Center, Cold Spring Harbor Laboratory. All rights reserved.
