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Differential Gene Expression Differential Gene Transcription What are the major differences between prokaryotic and eukaryotic genes? Differential Gene Transcription Differential Gene Transcription Differential Gene Transcription Differential Gene Transcription Promoters How do promoters work? Enhancer How is an enhancer different from a promoter? Activates use of promoter Activates promoters on same strand Doesn’t need to be adjacent to gene Doesn’t need to be upstream Enhancer How can one identify presence of an enhancer? Why are they so important? Transcription Factors What are the major structural domains of these molecules? DNA-binding domain Trans-activating domain Protein-protein interaction domain Transcription Factors What is MITF? Trans-activating domain Protein-protein interaction domain DNA-binding domain DNA Transcription Factors Why is PAX6 such an important molecule? Silencers What are silencers? Transcriptional Control Of what value is DNA methylation? How has this been demonstrated experimentally? How does methylation work? Transcriptional Control What are insulators? Transcriptional Control How does dosage compensation influence transcription? What are Barr bodies? How does this relate to calico cats? RNA Processing What is the difference between nRNA and mRNA? RNA Processing How is differential splicing of nRNA accomplished? What is the difference between genome and proteome? Translational Control What are some of the factors that influence gene expression at the level of translation? Translational Control Posttranslational Regulation What is meant by this level of regulation? How can it occur? Controlling Gene Expression Controlling Gene Expression Controlling Gene Expression