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Name __________________________________ Date_________ Period _______ Living Environment: DNA Fingerprinting (2) Aim: How do scientists identify people using DNA Fingerprinting? 1) Why do we fingerprint? ____________________________________________ 2) Why is DNA used to identify people? _________________________________ 3) What is a Genetic Marker? _________________________________________ _________________________________________________________________ 4) How do scientists make a DNA Fingerprint? a) Where can scientists find the DNA? 1- _______________, 2-______________, 3-______________, 4- ______________ b) What if you find only a little DNA? __________________________________________________ _______________________________________________________________________________ 5) What is Gel Electrophoresis: _________________________________________________________ 6) What are Restriction Enzymes: _______________________________________________________ Steps of Gel Electrophoresis: 1) ____________________________________ ____________________________________ 2) ____________________________________ ____________________________________ 3) ____________________________________ ____________________________________ ____________________________________ 4) ____________________________________ ____________________________________ ____________________________________ 5) ____________________________________ ____________________________________ ____________________________________ 6) _______________________________________________________________________________ _______________________________________________________________________________ 1 DNA Fingerprint Questions: 1) What is the charge of the DNA? _______________________________________________________ 2) Where would we find the smallest DNA fragments? ________________________________________ __________________________________________________________________________________ 3) Why do we get different banding patterns for each individual? _______________________________ __________________________________________________________________________________ 4) Which suspect was at the crime scene? __________________________________________________ ------------------------------------------------------------------------------------------------------------------------------1. Base your answer to the question on the diagram below and on your knowledge of biology. The diagram shows the results of a technique used to analyze DNA. This laboratory technique is known as: A. B. C. D. gel electrophoresis DNA replication protein synthesis genetic recombination Base your answers to following 3 questions on the information below and on your knowledge of biology. In an investigation, DNA samples from four organisms, A, B, C, and D, were cut into fragments. The number of bases in the resulting DNA fragments for each sample is shown below. 2. The diagram above represents the gel-like material through which the DNA fragments moved during gel electrophoresis. Draw lines to represent the position of the fragments from each DNA sample when electrophoresis is completed. [1] 3. Which two DNA samples are the most similar? Support your answer using data from this investigation. _____________________________________________________________________________________ 4. State two specific uses for the information obtained from the results of gel electrophoresis. [1] ____________________________________________________________________________________ 2