Download DNA Fingerprinting Notes - Hicksville Public Schools

Name __________________________________
Date_________
Period _______
Living Environment: DNA Fingerprinting (2)
Aim: How do scientists identify people using DNA Fingerprinting?
1) Why do we fingerprint? ____________________________________________
2) Why is DNA used to identify people? _________________________________
3) What is a Genetic Marker? _________________________________________
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4) How do scientists make a DNA Fingerprint?
a) Where can scientists find the DNA?
1- _______________, 2-______________, 3-______________, 4- ______________
b) What if you find only a little DNA? __________________________________________________
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5) What is Gel Electrophoresis: _________________________________________________________
6) What are Restriction Enzymes: _______________________________________________________
Steps of Gel Electrophoresis:
1) ____________________________________
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2) ____________________________________
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3) ____________________________________
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4) ____________________________________
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5) ____________________________________
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6) _______________________________________________________________________________
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DNA Fingerprint Questions:
1) What is the charge of the DNA? _______________________________________________________
2) Where would we find the smallest DNA fragments? ________________________________________
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3) Why do we get different banding patterns for each individual? _______________________________
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4) Which suspect was at the crime scene? __________________________________________________
------------------------------------------------------------------------------------------------------------------------------1. Base your answer to the question on the diagram below and on
your knowledge of biology. The diagram shows the results of a
technique used to analyze DNA.
This laboratory technique is known as:
A.
B.
C.
D.
gel electrophoresis
DNA replication
protein synthesis
genetic recombination
Base your answers to following 3 questions on
the information below and on your knowledge of
biology.
In an investigation, DNA samples from four
organisms, A, B, C, and D, were cut into
fragments. The number of bases in the resulting
DNA fragments for each sample is shown below.
2. The diagram above represents the gel-like material through which the DNA fragments moved during
gel electrophoresis. Draw lines to represent the position of the fragments from each DNA sample
when electrophoresis is completed. [1]
3. Which two DNA samples are the most similar? Support your answer using data from this
investigation.
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4. State two specific uses for the information obtained from the results of gel electrophoresis. [1]
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