Download Human Genome Project and Gene Therapy Overview

Human Genome Project and Gene Therapy Overview
HUMAN GENOME PROJECT
*An international effort among scientists to make a map of all of the human genes.
Go to https://www.youtube.com/watch?v=_EK3g6px7Ik and watch the video on the human genome
project. You can also google “Exploring Our Molecular Selves Human Genome Project.” Answer the
following questions as you watch.
1.
Does every cell in your body contain ALL of your genes?
2.
What do we call the complete set of all of our genes?
3. What are our chromosomes made of?
4. Who does DNA give its message to?
5. Where does the mRNA go when it leaves the nucleus?
6. What is built at the ribosome?
7. Name two things that proteins can do/be?
8. How many letters (nitrogen bases) have scientists “read” to help map the human genome?
9. “Different genes can influence your looks, personality and your risk for
______________________ , like _______________ and heart disease.”
Go to http://www.ornl.gov/sci/techresources/Human_Genome/home.shtml
1.
What was the human genome project?
2.
What were the goals of the human genome project?
GENE THERAPY
*Inserting a healthy gene into an individual who has a faulty gene.
Now that all of the genes in the human genome have been mapped, scientists and doctors can use this
information to perform GENE THERAPY.
1.
Read the Case study on Rhys Evans (I gave it to you as a handout).
a.
What is SCID?
b. Where did doctors put the healthy (appropriate gene) in Rhys’ body?
c. Was Rhys’ gene therapy successful?
d. What happened to the French boys who underwent the same gene therapy?
e. Explain how the inserted gene caused cancer.
f.
How did these two boys influence the future of gene therapy with this type of virus?
2. Watch the video on Gene Therapy –
You can also google the following, and choose the first video.
“Understanding: Gene Therapy Treatment: How Stuff Works”
http://www.bing.com/videos/search?q=power+of+genes+gene+therapy&view=detail&&&mid=
690E43A4B7A9BAE4873A690E43A4B7A9BAE4873A&rvsmid=690E43A4B7A9BAE4873A690E43A
4B7A9BAE4873A#view=detail&mid=690E43A4B7A9BAE4873A690E43A4B7A9BAE4873A
a. What was wrong with John Scanlon’s heart?
b. How did doctors deliver the VEG2F gene into his heart?
c. What did the gene do for John Scanlon?
d.
Was this gene therapy treatment successful?
GEL ELECTROPHORESIS
*Creating a DNA fingerprint using a gel chamber.
http://learn.genetics.utah.edu/content/labs/gel/
Make a prediction:
1. How do you think you could figure out the lengths of the strands in the tube of DNA?
Go through the simulation:
2. What is the process called in which we measure the DNA microscopically?
3. What is the “gel”?
4. Write down the step of gel electrophoreses
a.
b.
c.
d.
5. What does the current do the DNA samples?
6. What kinds of strand move quickly and further down the gel?
7. What kinds of strand move slower and lag behind?
8. What about the strand that are the same length?
9. What helps us see the DNA strand in the gel?
10. What are the ingredients to make a gel? Make your Gel!
11. Load the Gel with the DNA!
12. After you load the DNA sample into the tray, what is the next step?
13. How do you know current is running through the gel?
14. After the gel is done, what must you do to it before you can analyze your results?
15. How long does this process take?
16. What type of light do you use to view the gel? Is it safe and what precautions would you might
need to use?
17. In the space at rightdraw your gel
18. Write your size estimates below:
a. Strand 1___________________
b. Strand 2 ___________________
c. Strand 3 ___________________
19. Could you list one reason why we would run
a Gel electrophoresis on someone and
explain your answer.