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Kenneth Wu Eddie Johnson Yoni Aboody Ch. 15-20 Genetics Experiments of Griffith and Avery (bacteria Transformation), Hershey and Chase (phosphate in DNA), Watson and Crick (Structure of DNA), Nelson and Stahl (DNA replication) Base pairing rule (A-T, G-C) in RNA (A-U, G-C) How the DNA replicates One-gene-one polypeptide hypothesis Process of protein synthesis (transcription- mRNA- Translation- tRNAribosomes) Start codon (AUG) Metholine and stop codon (UAA, UGA, UAG) Splicing of RNA in eukaryotic cells (introns out, exons stay) GTP not ATP cause chain if protein Know what a virus is and how a virus replicate (lytic and lysogenic cycle) Viral Structure and type genetic (DNA single, double, RNA single) Reverse Transcriptase (HIV and AIDS) Viroid and Prions Bacteria having circular DNA and plasmid (secondary DNA) Ways how DNA is transferred is bacteria (transformation, transduction, and conjugation) Transposons and how they work Operons and how they work Know that a mRNA has 5’ cap and Poly-A tail in eukaryotes 6. Which of the following best describes the parents in a testcross? (1999.9) a. One individual has the dominant phenotype and the other has the recessive phenotype. b. Both individuals are heterozygous. c. Both individuals have the dominant phenotype. d. Both individuals have the recessive phenotype. e. Both individuals have an unknown phenotype. 15-19. Refer to the following list to answer the following questions. The answers may be used once, more than once, or not at all. (1999.92-95) a. Transcription b. Translation c. Transformation d. Replication e. Reverse Transcription 15. Process in which a protein is assembled at a ribosome. 16. Process in which naked DNA is taken up by bacterial or yeast cell 17. Process in which RNA is produced by using a DNA template. 18. Process that results in the production of cDNA from an RNA molecule. 19. Process in which DNA is produced by using a DNA template 22. Which of the following is an additional use of the gel electrophoresis technique? a. To express a gene b. To separate proteins in a mixture c. To ligate DNA fragments d. To transform E. coli e. To amplify genes 2. Meiosis reduces chromosome number and rearranges genetic information. a. Explain how the reduction are accomplished in meiosis. b. Several human disorders occur as a result of defects in the meiotic process. Identify ONE such chromosomal abnormality, what effects does it does it have on the phenotype of people with that disorder? Describe how this abnormality could result from a defect in meiosis. c. Production of offspring by parthenogenesis or cloning bypasses the typical meiotic process. Describe either parthenogenesis or cloning and compare the genomes of offspring with those of the parents.