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Due Wednesday 5/12/10
Ch 11 homework
1. The term "gene expression" refers to the (.5)
A) fact that each individual of a species has a unique set of genes.
B) fact that individuals of the same species have different phenotypes.
C) process by which genetic information flows from genes to proteins.
D) fact that certain genes are visible as dark stripes on a chromosome.
E) flow of information from parent to offspring.
2. Outline the function of the lac operon when no lactose is present. (1)
3. What is differentiation? At what stage of life does most cellular differentiation occur?
(1)
4. Explain alternate RNA splicing. How does it enable a single gene to encode more than
one kind of polypeptide? (1)
5. What are oncogenes, proto-oncogenes, and tumor-suppressor genes? (1)
6. Explain how DNA is packaged into chromosomes in Eukaryotes (1).
7. Enhancers are (.5)
A) adjacent to the gene that they regulate.
B) required to turn on gene expression when transcription factors are in short supply.
C) the site on DNA to which activators bind.
D) required to facilitate the binding of DNA polymerases.
E) the products of transcription factors.
8. Outline the 4 ways genes expression can be regulated after mRNA has been processed
and transported to the cytoplasm. (2)
9. Outline how a signal transduction pathway works to control gene expression (1).
10. Compare and contrast reproductive and therapeutic cloning (1).
Ch 12 Questions
1. When DNA from two sources is combined into one single piece of DNA, it is known
as (1)
A) cloned DNA.
B) recombinant DNA.
C) a vector.
D) a plasmid.
E) a DNA library.
2. DNA ligase binds (1)
A) exons together.
B) polymerase to the promotor.
C) nucleotides together.
D) introns together.
E) an intron to an exon.
3. Outline how PCR works (2)
4. What are restriction enzymes and how do they work?(1)
5. When performing electrophoresis, which end of the gel (positive or negative) will long
fragments “run” to? What about short segments? What causes this difference? (2)
6. The feature of "sticky ends" that makes them especially useful in DNA recombination
is their ability to (1)
A) bind to DNA and thereby activate transcription.
B) bind to ribosomes and thereby activate translation.
C) form hydrogen-bonded base pairs with complementary single-stranded stretches of
DNA.
D) allow plasmids to attach to the main bacterial chromosome.
E) insert a segment of RNA into a bacterial chromosome.
7. What is recombinant DNA? (1)
8. How do you make cDNA from mRNA? Outline the process. (1)