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A DAY - Thursday, 4/12 B DAY – Friday, 4/13 DNA REVIEW GUIDE Read Ch. 12 Carefully review class notes, diagrams & other handouts Review all items in homework folder Focus points 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. Describe Friedrich Miescher’s (1869) contribution to science. Review Frederick Griffith’s experiment (1928) in detail. Why did S cells maintain ability to synthesize capsules while R cells could not? What does the term transformation mean in terms of DNA? Describe/diagram Avery et. al (1944) experiment. Describe and diagram the experiment performed by Alfred Hershey and Martha Chase in 1952? What did this experiment conclude What instrument or technique was used by Rosalind Franklin to determine the structure of DNA? Draw a diagram of how this technique works. ExplainJames Watson and Francis Crick contribution to biology? List the 3 parts of a DNA nucleotide. What are the 4 nucleotide bases of DNA? List Chargaff’s Rules (1947). What did Chargaff’s research help Watson and Crick deduce about DNA’s configuration? List the purines. List the pyrimidines. Describe the hydrogen bonding between the various nitrogen bases. What molecules make up the backbone of the DNA molecule? What does the term semiconservative mean? What about antiparallel? Describe the function of each enzyme associated with DNA: helicase, DNA polymerase, DNA ligase, topoisomerase, editase). Explain in detail how DNA replicates. Completely discuss how transcription works, elaborating on how DNA strand, mRNA, and RNA polymerase are involved. (if you need additional help see textbook - page 301 and 304) List the four differences between RNA and DNA. Describe function and location in the cell of the three types of RNA (page 301302). What is a polypeptide and what kinds of bonds are associated with it? What does translation mean in reference to genetics? (Be able to translate DNA and RNA sequences into amino acid sequences on the test). Explain codons and anticodons. Review concepts PCR, gel electrophoresis and DNA typing/probes from HW assignments.