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Transcript
DISCOVERY OF DNA Structure and Function Ancient Ideas on Genetics Father of Modern Genetics Gregor Mendel (1822-1884) Fred Griffith’s Transforming Principle (1928) • Experimented with two strains of Pneumococcus bacteria Smooth: S- strain With capsule Rough: R-strain No capsule Heat –killed S-strain Combine live R-strain with Heat-killed S-strain Where is the Conveyer of Inheritance (transforming principle)? Protein or DNA Thomas Morgan (1911) Oswald Avery et al. (1944) 1. 2. The Experiment Treated the bacteria with centrafugation, which eliminates large cellular pieces. Added protease, which removes all proteins The result: 3. Treated with deoxyribonuclease, which eliminates all DNA The result: Alfred Hershey-Martha Chase Experiment (1952) Use Radioactively labeled viruses 2 types How can they be used to discover what is the conveyer of inheritance? 32P 35S DNA or Protein???? http://highered.mcgrawhill.com/olc/dl/120076/bio21.swf STRUCTURE OF DNA Miescher (1869) looked at pus cells Levene (1928) Different types of nucleotides: A, T, G, C What is the implication of this structure? Erwin Chargaff (1949) Rosalind Franklin (1950s) Implications? Watson and Crick 1953 Read the Nature article by Watson & Crick Discuss how they used the work of others to come up with the structure of DNA. How does the structure account for identical replication of DNA to be the conveyer of inheritance?
