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Transcript
Continuing from last time: Receptors 2 types of acetylcholine receptors: same ligand different response Glutamate Non-NMDA Mg++ 2 types of glutamate receptors NMDA Na+ Na+ Removal of Mg++ Explain response Coincidence detector Na+ Ca++ Na+ Na+ Na+ Ca++ Adapted from fig 21-40 Moving On: Neurotransmitters Soluble Neurotransmitter receptors Transmembrane Synaptic signaling requires protein receptors and peptide- derived signals How do you make a protein? How do you get a protein where it needs to be? Questions Part I: soluble neuropeptide –From DNA to protein What is the central dogma? How would a neuropeptide get made (in general terms)? What are the basic parts of DNA, RNA, and proteins? What is the difference between hnRNA, mRNA and tRNA? Synthesis overview What carries the information? Polymerization of nucleotides Genes ‘ The entire nucleic acid sequence necessary for the synthesis of a functional polypeptide’ MCB, p285 Components of a Eukaryotic Gene What are the ‘parts’ or regions of a gene? How are they organized? Do genes always encode RNAs? Do genes always encode RNAs that encode proteins? Questions How does RNA polymerase work and what does it make? How does it know where to start and stop? How does a ribosome work and what does it make? How does it know where to start and stop? If the DNA in every cell in your body is the same why don't your adipose (fat) cells secrete epinephrine? If the DNA contains all of the information why doesn't the ribosome just 'read' it? Why have intermediate steps? Looking at RNA ECB2 7.1 Making a cytosolic protein: Step 1--transcribe Genes encode proteins Why use an RNA step? Major steps in process: Initiation, Elongation and Termination animation Initiation DNA-RNA interactions DNA Template strand Complementary RNA strand DNA/RNA Hybrid Watching Transcription EBC2 7.2 Adapted from Life; Purves 6thed http://www.stolaf.edu/people/giannini/flashanimat/molgenetics/transcription.swf http://www.johnkyrk.com/DNAtranscription.html Check out Synthesis overview And then there was: a)Processing b)Translation
