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Chapter 7 Cell Structure and Function (Aligned with 7.1 Intro Sheet and 7.2 Cell Structure Chart) IMPORTANT: My hope is that you will use this on your computer as a way to ensure that your chart is filled in completely. If you MUST print it out, please make sure you print SIX SLIDES PER PAGE!! Save a tree!! 7-1 Life is Cellular A. Cells – basic units of structure and function in living things B. Early scientists that led to the cell theory Anton van Leeuwenhoek (1600s) – given credit for developing the 1st mini microscope, looked at pond water and made detailed drawings Robert Hooke – coined the term “cell” when he looked at slices of cork and dead plant cells Robert Brown (1833) – observed a dark structure near the center of the cell (we now know this is the nucleus) Matthias Schleiden (1838) – stated all plants are made of cells Theodore Schwann (1839) – stated all animals are made from cells Rudolph Virchow (1855) - stated all cells come from the division of preexisting cells Lorenz Oken – stated all new cells are the result of division of preexisting cells…VIRCHOW STOLE HIS THUNDER C. Cell Theory All living things are composed of cells. Cells are the basic units of structure and function in living things. All cells come from preexisting cells. Exploring the Cell : Microscopes * Lenses used to magnify image by focusing beams of light OR electrons The electron microscope (EM): • Allows greater magnification and reveals cellular details • Magnify cells up to 2 mill X • Limits: no living specimens LM 1,000 The light microscope (LM): • Enables us to shape and structure of a cell • Magnify cells up to 1,000X * Resolution = clarity of detail TEM 2,800 Magnification = image size SEM 2,000 • Figure 4.1B Figure 4.1C Scanning EM: (SEM) surface structures Figure 4.1D Transmission EM: (TEM) inside details Why are cells so small? A small cell allows a sufficient surface area to allow nutrients and wastes to cross per it’s volume. (in other words: it can’t get too big for it’s own good) volume= 27,000 volume= 27,000 Ratio: .6 to 1 Ratio: .2 to 1 need as high a surface area to volume ratio as possible What is in a Cell? ALL cells: * contain cytoplasm, cell membrane, and DNA * are either a prokaryotic or a eukaryotic Prokaryote Eukaryote •The smallest cells in nature •DNA not contained in a nucleus, free in the cytoplasm •Ribosomes are the ONLY cell organelle •Who are they? Bacteria •Much larger and more complex •DNA is wrapped with a nuclear membrane (nuclear envelope) •MANY more cell organelles •Who are they? Protists, Fungi, Plants, Animals 7-2 Cell Structure A. Cellular Control Center • Nucleus, nucleolus, chromatin/chromosomes, nuclear membrane/pore B. Organelles that Store, Clean Up and Support • Vacuoles, vesicles, lysosomes, cytoskeleton (microtubules, microfilaments), centrioles C. Organelles that Build Proteins • Ribosomes, endoplasmic reticulum (smooth and rough), golgi apparatus D. Organelles that Capture and Release Energy • Chloroplasts, mitochondria E. Cellular Boundaries • Cell wall, cell membrane F. Fluid Portion Outside of Nucleus (Sort of a boundary!) • cytoplasm CELLULAR CONTROL CENTER: NUCLEUS Function: Information center of the cell Contains DNA (chromatin vs. chromosomes) Directs cell activities 2 categories of organisms Prokaryotes – organisms whose cells lack nuclei (i.e. bacteria) Eukaryotes – organism whose cells contain nuclei Nucleus Structure: NUCLEOLUS – a small, darkened region in the nucleus that is made up of RNA and proteins, this is where ribosomes are made CHROMOSOMES – large structures formed from DNA that contain the genetic info CHROMATIN – uncondensed DNA found in non-dividing cells NUCLEAR ENVELOPE – double membrane around the nucleus that contains pores, allows molecules to move in and out of the nucleus, and protects the nucleus NUCLEAR PORES – allows passage of materials into or out of nucleus (RNA, ribosomes) Nucleus ORGANELLES THAT STORE, CLEAN UP, SUPPORT: VACUOLE Structure: Large, central structure in plants Many, small, circular structures in animal cells Filled with liquid Function: Storage of water, salts, proteins, carbohydrates, waste products Pressure system for plants, prevents wilting Special case: contractile vacuole - prevents excess water intake, leading to cell-bursting ORGANELLES THAT STORE, CLEAN UP, SUPPORT: VESICLE Store and move materials between cell organelles and to/from cell surface ORGANELLES THAT STORE, CLEAN UP, SUPPORT: LYSOSOME Structure: Small, circular structures Found only in animal cells Contain digestive enzymes Function: Digestion of: Worn out organelles Debris Large ingested particles Lysosomes are responsible for your hands not being webbed!! Lysosome ORGANELLES THAT STORE, CLEAN UP, SUPPORT: CYTOSKELETON Structure: Hollow tubes of proteins Examples: cilia (cells lining tracea), flagella (sperm cells), centrioles Function: Framework Provide cell with support, structure and shape Movement (cilia, flagella) Microfilaments – made of actin threads,allow movement of cytoplasm within the cell (cytoplasmic streaming) Microtubules – hollow structure made of tubulin, maintain ORGANELLES THAT STORE, CLEAN UP, SUPPORT: CENTRIOLES Organize cell division ORGANELLES THAT STORE, CLEAN UP, SUPPORT: PLASTID Structure: Differ based on type of plastid (chloroplast is one example) Found only in plants Function: Store food/starch Store pigments (give color to fruits & veggies) ORGANELLES THAT BUILD PROTEINS: RIBOSOMES Structure: Small (25 nm) ball-like structures Found free-floating in cytoplasm or attached to rough endoplasmic reticulum Composed of RNA and protein Make peptide bonds between amino acidsprotein Function: Synthesis of proteins (where proteins are made) ORGANELLES THAT BUILD PROTEINS: ENDOPLASMIC RETICULUM Structure: Network of flattened sacs Can be rough (w/ ribosomes) or smooth (w/o) Function: Transport materials within or out of cell Synthesis of macromolecules Rough - proteins, lipids, carbs Smooth - lipids ORGANELLES THAT BUILD PROTEINS: GOLGI APPARATUS Structure: Flattened stacks of membranes Vesicles attached to top and bottom Function: Collection, modification, packaging of proteins and other substances Vesicles attach, deposit materials GA modifies materials based on needs Vesicles attach to membrane and distribute modified substances ORGANELLES THAT CAPTURE/RELEASE ENERGY: CHLOROPLAST Structure: Double membrane Elaborate structure inside Function: Another power station Found in plant cells only Conversion of light energy (sun) into chemical energy (glucose) ORGANELLES THAT CAPTURE/ RELEASE ENERGY: MITOCHONDRIA Structure: Double membrane Cristae - inner folds, increase surface area Outer membrane for protection of cell Function: “Powerhouse” of the cell Able to self-replicate ( # in cells with high energy need) Converts sugars into energy for cells CELLULAR BOUNDRIES: CELL WALL Only in plants, algae, and some bacteria Lies outside the cell membrane Function Helps to protect and support the cell Very porous (water, oxygen, carbon dioxide, etc. can pass through easily) Gives rectangular shape to plant cells Layers 1st layer – contains pectin (gluey substance that helps hold the cells together) 2nd layer – primary cell wall (made of cellulose) 3rd layer (in woody stems) – secondary cell wall (composed of cellulose and lignin to make cellulose more rigid) CELLULAR BOUNDRIES: CELL MEMBRANE – outer boundary Structure: Phospholipid bilayer • hydrophilic heads, hydrophobic tails Contains lipids (bilayer), proteins (channels), and carbohydrate chains (identification cards) Function: Regulates what enters and leaves the cell Semi-permeable membrane Protection and support Cell/Plasma Membrane CELLULAR BOUNDRIES: CYTOPLASM Material between the cell membrane and the nucleus Contains the organelles of the cell Plant vs. Animal Cell Animal Cell Plant Cell QUESTIONS: Describe the steps involved in the synthesis, packaging, and exporting of a protein from a cell. What are the two major parts of the cell? How do contractive vacuoles help maintain water balance? What is the difference between rough and smooth ER? Why is the cell membrane sometimes referred to as a fluid mosaic? What part of the cell membrane acts like a fluid? And what makes it like a mosaic? How do the properties of lipids help explain the structure of the cell membrane? Why do you think it’s important that cell membranes are selectively permeable? QUESTIONS: Describe the steps involved in the synthesis, packaging, and exporting of a protein from a cell. Proteins assembled on ribosomes (if targeted for export to cm or to specialized locations w/in cell, complete their assembly on RER protein in vesicle Golgi apparatus (further modifies proteins before sorting and packaging them in membrane bound vesicles) vesicle final destination What are the two major parts of the cell? Cytoplasm with organelles, and nucleus How do contractive vacuoles help maintain water balance? Pump out excess water What is the difference between rough and smooth ER? Rough has ribosomes, smooth does not Why is the cell membrane sometimes referred to as a fluid mosaic? What part of the cell membrane acts like a fluid? And what makes it like a mosaic? It is made of many parts (like a mosaic) that can float around in the fluid phospholipid bilayer How do the properties of lipids help explain the structure of the cell membrane? Hydrophilic lipid heads are attracted to water, hydrophobic fatty acid tails turn away from water. A bilayer forms when heads turn outward towards water inside and outside a cell Why do you think it’s important that cell membranes are selectively permeable? Allows needed substances to enter and wastes to leave, while keeping molecules that are not needed out