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Cell Theory A theory that states: 1. All cells come from other cells 2. Cells are the basic unit of all living things 3. Cells are the smallest living thing. Compound Light Microscope A microscope with two lenses: the objective that magnifies the sample and the ocular or eyepiece that magnifies the image from the objective 10x. Scanning electron Microscope A scope that produces an image by sending electrons across the surface of the sample. The image is 3-D and of the surface of objects. Electron gun Transmission Electron microscope • A scope that uses electron to produce a 2-D image, very detailes, the electrons are transmitted through a sample, the sample must be a thin , small preparation. Wet Mount Slide *A preparation of a sample on a slide. *Place a drop of water on a slide,place the sample in the water and slowly place on the coverslip at an angle to avoid bubbles Cover slip Water Sample in water No bubbles Prokaryote *Cells that do not have a nucleus or membrane bound organelles. *“Primitive” cells Example: Bacteria A bacterium Eukaryote *Cells that have membrane bound organelles ( little organs) *very organized with each organeele having a specific function *Complex animal, plant, fungi and Protista Plasma membrane or Cell Membrane • regulates the flow of materials into and out of cell, protection for the cell, gives the cell shape. • Outer layer of animals cells • The layer after the cell wall in plants Nucleus *The organelle that contains DNA (In the form of chromosomes) *Considered the control center of the cell • The organelle that is usually visible with a microscope. Nuclear envelope or Nuclear Membrane • double layer around nucleus, regulates movement into and out of nucleus Nuclear pore This would be like Taking the Envelope out To see it. DNA and Nucleolus would Be here Nucleolus • Literally means “little nucleus” • Located inside the nucleus • Production of RNA is here nucleolus nucleus Rough Endoplasmic reticulum (ER) *A complex of tube-like membranes and channels used to make and Transport materials in cell. *rough ER has ribosomes on the outside that produce proteins. *Proteins are produced and transported This part Golgi complex or Golgi Body • smooth stack of flatten membranes that resemble a stack of pancakes. *Function in storage and packaging of materials • Send out vesicles for various functions Smooth Endoplasmic reticulum (ER) * A complex of tube-like membranes used to produce and transport lipids in cell This part Without ribosomes (dots) Mitochondria • It is called the "power house“ of the cell • aerobic respiration occurs here that produces energy called ATP • Oval-like structure with folded membranes called cristae. Image from an Electron microscope Lysosomes • Small sac- like vesicl *digestive sacs contains enzymes that destroy foreign material or cell itself. Microfilaments/Microtubules *Cilia • Small hair-like structures for movement or pushing in food for some cells. • Many and very short and small The fuzzy stuff Microfilaments /Microtubules Flagella • • • • For movement in cells May be one or just several of these Large and long Whip-like Cytoskeleton • A system of tubules and filaments that provide support, hold organelles in place. • Look like long fibers, web-like Microfilaments /Microtubules Centrioles • Only in animal cells • Assist in cell division by producing spindle fibers for moving chromosomes during cell division • Look like a bundle of straws, there are always two at right angles to one another Cell Wall • Think layer of cellulose surrounding the plant cell for protection and support. This outer layer Chloroplast • Use sunlight to produce glucose in a process called photosynthesis • contains the green pigment, chlorophyll that absorbs the sunlight • Only in plants • Oval with membranes inside that look like a stack of coins. ( the coin like things are called thylacoids and a stack of these is called grana) The green items Differences in Animal and Plant Cells Animal cells: Plant cells: • tend to be irregularly shaped *Tend to be rectangular;connect or spherical(are able like brinks and do not conform to conform to others) or fold/bend to on another. • Have centrioles that form *have one large vacuole spindles for cell division * Cell wall made of cellulose • Have small vacuoles *no centrioles, but the • Only a cell membrane, no wall cytoskeleton forms spindles for cell division Vacuole • Large sac-like structure • Stores mostly water and minerals, but also can store food. • Very large in plants ( fills most of the cell) and small vesicles in animal cells Diffusion • The process by which molecules move from an area of greater concentration to an area of lesser concentration Osmosis • The diffusion of water across a selectively permeable membrane water membrane Active Transport • The movement of a substance across a cell membrane against a concentration gradient; requires the cell to expend energy Passive Transport • The movement of substances across a cell membrane without the use of cell energy • Examples are diffusion and osmosis (a type of diffusion) Exocytosis • A process in which a vesicle inside a cell fuses with the cell membrane and releases its contents to the external environment inside outside Endocytosis • The process by which a cell surrounds and engulfs substances Homeostasis • The stable internal conditions of a living thing • Being able to maintain this balance • Taking in nutrients, excreting waste, keeping temperature balance and water balance…) Equilibrium • A state that exists when the concentration of a substance is the same throughout a space Equal in And out Hypertonic • Describing a solution whose solute concentration outside is higher than that inside a cell; a cell will shrink due to loss of water. Metabolic Rate • Rate of metabolism; the amount of energy expended in a given period • The sum of all the chemical reactions: some require energy and some release it Isotonic • Describing a solution whose solute concentration equals that inside a cell; cells remain unchanged due to already in balance. Hypotonic • Describing a solution whose solute concentration is lower than that inside a cell; causes a cell to swell due to bringing in water. Plasmolysis • When a plant cell losses water and shrinks • The cell wall remains intact, but the cell membrane pinches in bringing all the organelles together in a mass. Normal plant cell Loss of water Sodium Potassium pump • A type of Active transport where ATP or energy is required to move sodium out of the cell ( to keep low sodium balance inside the cell) and bring Potassium in (to keep high potassium in). This is needed for proper cell function, especially nerve cell/nerve impulses. Photosynthesis • The process of converting light energy to chemical energy in plants or organisms with chlorplasts. • Produces organic molecules(sugars…) Selectively permeable membrane • The cell membrane allows some particle to pass and others are too large. Location of Photosythesis Chlorophyll *The pigments in plants located in the thylacoid of chloroplasts *Captures the light energy from the sun and converts it to organic molecules in photosynthesis Chlorophyll Is in these Respiration in the Mitochondria Cellular Respiration • The process of taking the energy from organic molecules to transferring it to energy (ATP) for the cell. • Requires Organic molecules • Produces ATP, Water and Carbon dioxide • May or may not use oxygen • All Cells NEED to Respire Anaerobic respiration • Respiration where no oxygen is available (not required) • Produces only 2 ATPs (not efficient) • Usually types of soil on ocean bacteria and yeast Two Types: 1. Alcoholic fermintation ( in yeast) 2. Lactic acid fermentation in muscles Aerobic respiration • Requires oxygen to convert glucose to ATP for the cell • Produces 36 ATPs, Carbon dioxide and Water from on glucose • Takes place in the mitochondria • Most eukaryotic cells/organisms are aerobic + 36 ATP Lactic acid Fermentation • Occurs when muscle or other cells do not have enough oxygen. They convert to anaerobic respiration until more oxygen is available. • In the cell’s cytoplasm and not mitochondria • Very little ATP produced (2), carbon dioxide and lactic acid. Causes a burning sensation in muscles, weakness Alcoholic Fermentation •In some bacteria and yeast •Process used to produce alcohol, yogurt, cheeses, sauerkraut, vinegar, cider… •Does not take place in the mitochondria but only in the cytoplasm. *Produces small amount of ATP , Carbon dioxide, water and ethyl alcohol The tie between Photosynthesis and Respiration The products of Photosynthesis are used for Respiration. The products of Respiration are used for Photosynthesis. *plants respire and photosynthesis- they need to make the food for respiration. * Animals consume their food for respiration Consumers(Respiration) and Producers (Respiraiton and photosynthesis)