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Why is today an important day for science/the world? What is your favorite Halloween costume that you have had? The best that you’ve seen? What are 3 things from the last test that you found most confusing? Ch 6 - A Tour of the Cell All organisms are made of cells • The Cell Theory: – All living things are made of cells – Cells are the basic unit of structure & function in living things – All cells come from pre-existing cells Organelle: a cell part/structure with a specific job/function Cell Structures & Functions • Structures in the nucleus: – Nucleus: control center for the cell, made of proteins, enzymes, & nucleic acids; houses the cell’s genetic material & directs cell activities – Nuclear envelope/membrane: a semipermeable double membrane which encloses the nucleus & regulates which substances (molecules) enter and/or leave the nucleus – Nucleolus: provide materials to produce the ribosomes Cell Structures & Functions • Structures in the nucleus: – Chromosomes: made of DNA & contain the instructions for everything the cell makes; long strands composed of nucleotides, which contain the coded instructions for the accomplishment of everything the cell makes & does – Nucleic acids: organic compounds found inside & outside of nucleus of cells that carries instructions for cellular activity; DNA & RNA are types of nucleic acids Cell Structures & Functions • Structures outside the nucleus: – Cytoplasm: the combination of all the liquids, substances, & structures between the plasma (cell) membrane & the nucleus; consists of various organelles suspended in the fluid outside the nucleus – Cell/Plasma membrane: the membrane that’s the outer boundary of the cytoplasm; it is semipermeable & regulates the entry and/or exit of molecules between the cytoplasm & outside environment of the cell; a phospholipid bilayer containing proteins that form specific functions such as chemical reactions, cell-to-cell communication & cell signaling, and transport of materials Cell Structures & Functions • Structures outside the nucleus: – Endoplasmic reticulum (ER): the internal transportation system of the cell; a network of microscopic channels or canals formed from the cell membrane spreading throughout the cytoplasm – Ribosomes: small spherical structures composed of RNA (from the nucleolus); found on the rough ER & assemble proteins Cell Structures & Functions • Structures outside the nucleus: – Mitochondria: powerhouse of cell; release energy from sugars & other molecules (active cells such as muscle contain many of these); small oval or rod-shaped structures in the cytoplasm within which potential energy is converted to kinetic energy – Lysosomes: membrane-bound sacs that contain enzymes; which are used to break down macromolecules into smaller molecules (digestion); destroy harmful bacteria; used to recycle cell parts Cell Structures & Functions • Structures outside the nucleus: – Golgi apparatus: the packaging & distribution center of the cell; modifies, stores, & routes proteins & other chemical products to their next destinations (like a factory or post office); a system of storage chambers for molecules made within the cell but which are to leave the cell to be used somewhere other than in the cell that produced them Cell Structures & Functions • Structures outside the nucleus: – Vesicles: small membrane sacs that specialize in moving products into, out of, & within the cell – Chloroplasts: contain chlorophyll; is the site of photosynthesis, the chemical process which packages the energy of the sun into a carbohydrate (sugar) molecule; in plant cells only – Cell wall: made of cellulose; is produced by the cell for protection & strength; located immediately next to the outer surface of the cell membrane of plants only Cell Structures & Functions • Vacuoles: membrane-bound spaces (bag-like structures) found in the cytoplasm including the following: – Food Vacuole: stores particles of food – Water vacuole: stores water & may act as a kind of “pump” to force excess water out of cell – Digestive vacuole: encloses particles of food & chemically breaks down food molecules – Waste vacuole: contains useless leftover molecules from the digestive process – Contractile vacuole: pumps out excess water that diffuses into single-celled organisms; keeps them from bursting • Prokaryotic cell: cells without a nucleus or membrane bound organelles; bacteria • Eukaryotic cell: cells with a nucleus and membrane bound organelles; us/animals/plants Cell Posters Next! Pro vs. Eu • Eu = True • Pro = earleir than Membranes organize a cell’s activities • Cell membrane - Like security guard - controls flow of materials into & out. • Phospholipid - Basic building block of membranes - Type of lipid w/ polar head & nonpolar tail - Head - phosphorus & Nitrogen - POLAR • Phospholipid Bilayer - Phospholipids aligned in 2 layers - Polar heads point toward water 2 Important Characteristics of Membranes 1. Most polar molecules cannot cross. - Interior of membrane is barrier - Passageways of protein (“gates”) - Regulate what enters and leaves. 2. Phospho. & Proteins not fixed in place. - Mem. structured to fit needs of diff. cell types. 3 PROTEINS IN MEM. - Channels, Receptors, and Markers. • CHANNELS (transport) - Doughnutshaped. - Needed molecules & ions cross mem. through these. • RECEPTORS – Boulders - Convey info. from external environment. • MARKERS - Elongated w/ chains of carbs. - “Name Tags” • Proteins bend, fold, twist. Shape determines function. Functions of Membrane Proteins Cell Membrane Membrane Proteins have 3 Sections - 2 ends contain polar A.A. - form H-Bonds w/ water. - Middle - nonpolar A.A. anchored into membrane, remains mobile. End of 10/29 Membranes regulate the traffic of molecules. • Diffusion – net movement of substances from high to low concentration. • Equilibrium – Balance. Movement is = outside to inside Passive Transport • Passive – no Energy used. Ex: H2O • Facilitated diffusion – Transport proteins used as a pathway for diffusion (= to help). Ex: sugars • Osmosis – Passive Transport (diffusion) of H2O across selectively permeable membrane. – Hypertonic – solution w/ higher concentration (shrinks) Otonic – solution w/ lower concentration (swells) – Hyp – Isotonic – concentrations = (no change) How do different solutions affect plant & animal cells? Active Transport • Energy required to move molecules. - move from low to high concentration (against natural flow); Ex: ions • Endocytosis vs. Exocytosis End of 11/1 Journal – 11/14/11 30% H20 70% Salt 50% H20 50% Salt Beaker Why are cells so small? • It has everything to do with surface area and volume • Surface area: the measure of how much exposed area a solid object has (in squared units) • Volume: how much space a 3D object takes up (in cubed units) Why are cells so small? • Gases and food molecules dissolved in water must be absorbed and waste products must be eliminated • For most cells, this passage of all materials in and out of the cell must occur through the plasma membrane • Each internal region of the cell has to be served by part of the cell surface. As a cell grows bigger, its internal volume enlarges and the cell membrane expands Why are cells so small? • As a cell gets larger, the volume of the cell increases more rapidly than the surface area (if the cell maintains its same shape) • Because the volume increases more rapidly than does the surface area, the relative amount of surface area available to pass materials to a unit volume of the cell steadily decreases Video Why are cells so small? • Everything the cell needs or has to get rid of has to go through the cell membrane, the amount of which relates to the surface area. The cell's ability to either get substances from the outside or eliminate waste is related to the surface area • How much food and other material from the outside and how much waste the cell has to get rid of, is related to the volume • Therefore, as a cell gets bigger there will come a time when its surface area is insufficient to meet the demands of the cell's volume and the cell stops growing!