Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Document
Document related concepts
Biochemical switches in the cell cycle wikipedia , lookup
Tissue engineering wikipedia , lookup
Extracellular matrix wikipedia , lookup
Cell encapsulation wikipedia , lookup
Endomembrane system wikipedia , lookup
Cell nucleus wikipedia , lookup
Programmed cell death wikipedia , lookup
Cell growth wikipedia , lookup
Cell culture wikipedia , lookup
Cytokinesis wikipedia , lookup
Organ-on-a-chip wikipedia , lookup
Cellular differentiation wikipedia , lookup
Transcript
Transmembrane Signal Transduction Inflammatory mediators Induction of EGFP expression by 1 nM IL-1 in HeLa cells transiently transfected with a pIL8 reporter Minutes after IL1 IL1 vehicle EGFP Expression from pIL8-d2-EGFP in ten individual cells following IL-1 (1 nM) stimulation RelA Nuclear/CytoplasmicDistribution Response to IL-1 RelA Nuclear/CytoplasmicDistribution Heterogeneity Kinetic Analysis of IL-1 Induced p65/relA Nuclear Translocation Comments Cell to cell variations in both amplitude and kinetics of signal transduction events cause a significant noise in the system. Obtaining detailed information of regulatory mechanisms at the single cell level requires identification of fundamental controlling parameters. -------------To use the single cell data as the basis for mathematical modelling of signalling networks. To use the theoretical predictions for improved design of the biological experiment.
