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Transmembrane Signal Transduction
Inflammatory mediators
Induction of EGFP expression by 1 nM IL-1 in HeLa cells
transiently transfected with a pIL8 reporter
Minutes after IL1
IL1
vehicle
EGFP Expression from pIL8-d2-EGFP
in ten individual cells following
IL-1 (1 nM) stimulation
RelA Nuclear/CytoplasmicDistribution
Response to IL-1
RelA Nuclear/CytoplasmicDistribution
Heterogeneity
Kinetic Analysis of IL-1 Induced
p65/relA Nuclear Translocation
Comments
Cell to cell variations in both amplitude and kinetics of
signal transduction events cause a significant noise in the system.
Obtaining detailed information of regulatory mechanisms
at the single cell level requires identification of fundamental
controlling parameters.
-------------To use the single cell data as the basis for mathematical modelling
of signalling networks.
To use the theoretical predictions for improved design of the
biological experiment.