The p57 CDKi integrates stress signals into cellcycle progression to
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
... In contrast, the p57T143A mutant was not phosphorylated by p38 (Figure 3A). Importantly, incubation of the cells with the p38 SAPK inhibitor SB203580 precluded p57 phosphorylation indicating that in vivo p57 phosphorylation required p38 activation (Figure 3B). To rule out that p57 phosphorylation wa ...
Meclofenamic acid selectively inhibits FTO demethylation of m A
... m6 A-containing substrates (27). Both FTO and ALKBH5 are localized to nuclei and colocalize with nuclear speckles, indicating the effects of methylation on splicing. Indeed, knockdown of the ALKBH5 gene was shown to affect splicing in tissue culture cells, and displayed a sterility phenotype in male ...
... m6 A-containing substrates (27). Both FTO and ALKBH5 are localized to nuclei and colocalize with nuclear speckles, indicating the effects of methylation on splicing. Indeed, knockdown of the ALKBH5 gene was shown to affect splicing in tissue culture cells, and displayed a sterility phenotype in male ...
Damage response of XRCC1 at sites of DNA single strand breaks is
... progression are not well understood. We found that XRCC1 dissociates from pAR and is translocated to sites of SSBs dependent on its BRCTII domain and the function of PARG. In addition, phosphorylation of XRCC1 is also required for the proper dissociation kinetics of XRCC1 because (1) phosphorylation ...
... progression are not well understood. We found that XRCC1 dissociates from pAR and is translocated to sites of SSBs dependent on its BRCTII domain and the function of PARG. In addition, phosphorylation of XRCC1 is also required for the proper dissociation kinetics of XRCC1 because (1) phosphorylation ...
Direct Interaction between Survivin and Smac/DIABLO Is Essential
... Smac/DIABLO Is Released from the Mitochondria Accompanied with Cytochrome c upon Induction of Apoptosis by Taxol— Taxol is a natural product with potent anti-tumor activity and has been approved for the treatment of breast, ovarian, and lung cancers (25, 30 –32). We incubated HeLa cells with Taxol a ...
... Smac/DIABLO Is Released from the Mitochondria Accompanied with Cytochrome c upon Induction of Apoptosis by Taxol— Taxol is a natural product with potent anti-tumor activity and has been approved for the treatment of breast, ovarian, and lung cancers (25, 30 –32). We incubated HeLa cells with Taxol a ...
A bi-functional siRNA construct induces RNA
... Previously we have shown that routine transfection of E7 siRNA into SiHa cervical cancer cells induces growth arrest and apoptosis (11). In contrast, the growth and viability of nonvirally infected cells, such as normal human diploid fibroblasts and HCT116 human colorectal cancer cells, are unaffect ...
... Previously we have shown that routine transfection of E7 siRNA into SiHa cervical cancer cells induces growth arrest and apoptosis (11). In contrast, the growth and viability of nonvirally infected cells, such as normal human diploid fibroblasts and HCT116 human colorectal cancer cells, are unaffect ...
Characterization of the Nucleolar Gene Product, Treacle, in Treacher Collins Syndrome
... To further investigate the aberrant mobility of the putative treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 19 ...
... To further investigate the aberrant mobility of the putative treacle band on SDS-PAGE, we in vitro transcribed/translated treacle and analyzed it by SDS-PAGE and fluorography. For this purpose, we used the full-length cDNA of mouse treacle, which is 61.5% identical to human treacle (Dixon et al., 19 ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of
... lived and abnormal/misfolded proteins following labeling with Lys-48-linked polyubiquitin chains (1). The lysosome degrades extracellular and plasma membrane proteins brought there by endocytosis and cytoplasmic components delivered by autophagy. Various categories of autophagy have been defined dif ...
... lived and abnormal/misfolded proteins following labeling with Lys-48-linked polyubiquitin chains (1). The lysosome degrades extracellular and plasma membrane proteins brought there by endocytosis and cytoplasmic components delivered by autophagy. Various categories of autophagy have been defined dif ...
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... 1999; Hrycyna et al., 1998; Senior, Al-Shawi & Urbatsch, 1995). Numerous eukaryotic secreted and membrane proteins, including P-gp, contain N-linked glycosylation, or the addition of oligosaccharide side chains to asparagine (Asn) residues. N-linked glycosylation occurs when the Asn residue resides ...
... 1999; Hrycyna et al., 1998; Senior, Al-Shawi & Urbatsch, 1995). Numerous eukaryotic secreted and membrane proteins, including P-gp, contain N-linked glycosylation, or the addition of oligosaccharide side chains to asparagine (Asn) residues. N-linked glycosylation occurs when the Asn residue resides ...
Robust methods for purification of histones from cultured
... play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these processes. Linking specific histone PTMs to gene expres ...
... play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these processes. Linking specific histone PTMs to gene expres ...
Elevated Level of Nuclear Protein Kinase C in
... of added phospholipids and calcium and was expressed as '2P incorporated/min/mg protein. Colorimetrie MTT Test for Cytotoxicity to Adriamycin. MCF-7 cells were seeded in 24-well plates (Costar, Cambridge, MA) at a density of approximately 25,000 cells/well in 1MEM as described above. After 24 h the ...
... of added phospholipids and calcium and was expressed as '2P incorporated/min/mg protein. Colorimetrie MTT Test for Cytotoxicity to Adriamycin. MCF-7 cells were seeded in 24-well plates (Costar, Cambridge, MA) at a density of approximately 25,000 cells/well in 1MEM as described above. After 24 h the ...
Anti-HIV-1 activity of Trim 37
... Trim 37 expression and inhibition of HIV-1 replication Previous studies have suggested that Trim 37 is highly conserved and was expressed in all tissues tested (Hämäläinen et al., 2006; Kallijärvi et al., 2006). To verify these reports, we set out to characterize the expression levels of Trim 37 ...
... Trim 37 expression and inhibition of HIV-1 replication Previous studies have suggested that Trim 37 is highly conserved and was expressed in all tissues tested (Hämäläinen et al., 2006; Kallijärvi et al., 2006). To verify these reports, we set out to characterize the expression levels of Trim 37 ...
Cell-cycle-regulated expression of STIL controls centriole number in
... assembly of a so-called cartwheel structure, which presumably serves as an assembly platform for the outgrowth of the procentriole. Importantly, SAS-6 has recently been identified as a key component of the cartwheel (Nakazawa et al., 2007) and shown to confer ninefold symmetry to this structure (Kit ...
... assembly of a so-called cartwheel structure, which presumably serves as an assembly platform for the outgrowth of the procentriole. Importantly, SAS-6 has recently been identified as a key component of the cartwheel (Nakazawa et al., 2007) and shown to confer ninefold symmetry to this structure (Kit ...
Pathogenic potential of Aeromonas hydrophila isolated from surface
... Aeromonas spp. harbour both alt and ast genes, encoding heat-labile cytotonic and heat-stable cytotonic enterotoxin, respectively, associated with watery diarrhoea. The isolation rate of Aeromonas species from diarrhoeic stool has been reported to be as high as 10.8 %, compared to only 2.1 % from th ...
... Aeromonas spp. harbour both alt and ast genes, encoding heat-labile cytotonic and heat-stable cytotonic enterotoxin, respectively, associated with watery diarrhoea. The isolation rate of Aeromonas species from diarrhoeic stool has been reported to be as high as 10.8 %, compared to only 2.1 % from th ...
Isoform 5 of PIPKIc regulates the endosomal trafficking and
... determine whether these observations were due to changes in Ecadherin transcription, E-cadherin mRNA content was analyzed by quantitative (q)PCR (supplementary material Fig. S2B). The data indicated that there was no significant difference in E-cadherin mRNA levels upon expression of PIPKIci5 or SNX ...
... determine whether these observations were due to changes in Ecadherin transcription, E-cadherin mRNA content was analyzed by quantitative (q)PCR (supplementary material Fig. S2B). The data indicated that there was no significant difference in E-cadherin mRNA levels upon expression of PIPKIci5 or SNX ...
A Phosphorylation State-specific Antibody Recognizes Hsp27, a
... lags significantly behind knowledge of their regulatory mechanisms. However, recent technological advances in the identification of phosphorylation sites, such chemical genetics as well as mass spectrometry, have enabled progress in this area (3). In addition, the advent of phosphorylation state-spe ...
... lags significantly behind knowledge of their regulatory mechanisms. However, recent technological advances in the identification of phosphorylation sites, such chemical genetics as well as mass spectrometry, have enabled progress in this area (3). In addition, the advent of phosphorylation state-spe ...
Cotto J. Fox S. Morimoto R . HSF1 granules
... Similar results of co-localization were observed using HSF1GFP (Fig. 4). Whereas GFP alone is distributed in a diffuse pattern throughout the cell under control (Fig. 4A) and heat shocked conditions (Fig. 4B), mHSF1-GFP is primarily nuclear in control cells (Fig. 4C) and upon heat shock relocalizes ...
... Similar results of co-localization were observed using HSF1GFP (Fig. 4). Whereas GFP alone is distributed in a diffuse pattern throughout the cell under control (Fig. 4A) and heat shocked conditions (Fig. 4B), mHSF1-GFP is primarily nuclear in control cells (Fig. 4C) and upon heat shock relocalizes ...
Endocytic membrane fusion and buckling
... It has been proposed that the fusion of organelles with the furrow PM is important during the resolution of the ICB (Baluska et al., 2006). However, the function and timing of these fusion events remain controversial. Some studies suggest that asymmetric and synchronous fusion of secretory organelle ...
... It has been proposed that the fusion of organelles with the furrow PM is important during the resolution of the ICB (Baluska et al., 2006). However, the function and timing of these fusion events remain controversial. Some studies suggest that asymmetric and synchronous fusion of secretory organelle ...
Mitogen-activated protein kinase kinase 1
... 2004), we used siRNA-mediated knockdown of expression. Using a siRNA duplex targeting the 3⬘ untranslated region, we observed ⬃95% knockdown of a stably expressed HAtagged version of MEK1 in HeLa cells by immunofluorescence (our unpublished data) and immunoblotting (Figure 1A). To confirm knockdown ...
... 2004), we used siRNA-mediated knockdown of expression. Using a siRNA duplex targeting the 3⬘ untranslated region, we observed ⬃95% knockdown of a stably expressed HAtagged version of MEK1 in HeLa cells by immunofluorescence (our unpublished data) and immunoblotting (Figure 1A). To confirm knockdown ...
The RNase P Associated with HeLa Cell Mitochondria Contains an
... probes for 12S rRNA, ND1 mRNA, and COII mRNA were labeled by random priming (19), using [␣-32P]CTP, of plasmids containing a 12S rRNA gene fragment (between positions 764 and 1466) (5) (p12SSf), an ND1 gene fragment (between positions 3312 and 4121) (pKS2ND1), or a COII gene fragment (between positi ...
... probes for 12S rRNA, ND1 mRNA, and COII mRNA were labeled by random priming (19), using [␣-32P]CTP, of plasmids containing a 12S rRNA gene fragment (between positions 764 and 1466) (5) (p12SSf), an ND1 gene fragment (between positions 3312 and 4121) (pKS2ND1), or a COII gene fragment (between positi ...
Golgi-targeting sequence of p230 - Journal of Cell Science
... The location of p230 specifically to TGN membranes indicates the presence of a specific targeting sequence (Gleeson, 1998). In order to define the mechanism of action of p230, we have identified the sequence of p230 responsible for targeting to Golgi membranes. The studies reported here have identif ...
... The location of p230 specifically to TGN membranes indicates the presence of a specific targeting sequence (Gleeson, 1998). In order to define the mechanism of action of p230, we have identified the sequence of p230 responsible for targeting to Golgi membranes. The studies reported here have identif ...
1 Introduction of autophagy
... that p53 intensifies autophagy by acting on autophagy-related gene. Another study showed that it is in cytoplasm but not cell nucleus that p53 dampen-downs autophagy. • DAPK (death-associated protein kinase): we know that the combination of Beclin-1 and Bcl-2 attenuates autophagy. However, activated ...
... that p53 intensifies autophagy by acting on autophagy-related gene. Another study showed that it is in cytoplasm but not cell nucleus that p53 dampen-downs autophagy. • DAPK (death-associated protein kinase): we know that the combination of Beclin-1 and Bcl-2 attenuates autophagy. However, activated ...
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... surprising that they were isolated from the ethanol extract, typical of polyphenolic compounds with differential structure and generally hydrophilic character. This is only an apparent contradiction arising from the phenomenon of co-solution of compounds in rich, multi-component mixtures of natural ...
... surprising that they were isolated from the ethanol extract, typical of polyphenolic compounds with differential structure and generally hydrophilic character. This is only an apparent contradiction arising from the phenomenon of co-solution of compounds in rich, multi-component mixtures of natural ...
Phalloidin
... CF™ dyes are a series of next-generation fluorescent dyes developed at Biotium to have combined advantages in brightness, photostability, and water solubility compared to other fluorescent dyes. Fluorescently labeled phalloidins stain F-actin at nanomolar concentrations (1-3). Labeled phalloidins ha ...
... CF™ dyes are a series of next-generation fluorescent dyes developed at Biotium to have combined advantages in brightness, photostability, and water solubility compared to other fluorescent dyes. Fluorescently labeled phalloidins stain F-actin at nanomolar concentrations (1-3). Labeled phalloidins ha ...
Precipitation of calcium, magnesium, strontium and barium in tissues
... Morphologies and Locations of Crystals in Mature Plants Grown in the Field Prismatic crystals were observed in cells associated with fibre cells in phyllodes of all four species collected from mature plants grown in the field, and all prismatic crystals were birefringent (Fig. 1). There were also ot ...
... Morphologies and Locations of Crystals in Mature Plants Grown in the Field Prismatic crystals were observed in cells associated with fibre cells in phyllodes of all four species collected from mature plants grown in the field, and all prismatic crystals were birefringent (Fig. 1). There were also ot ...
STEROIDOGENESIS IN THECA CELLS OF CHICKEN FOLLICLES
... The aim of this study was to assess and compare the steroidogenic response to luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in cells of the theca interna and theca externa isolated from follicles at different stages of maturation. Cells of the theca interna and theca externa isolat ...
... The aim of this study was to assess and compare the steroidogenic response to luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in cells of the theca interna and theca externa isolated from follicles at different stages of maturation. Cells of the theca interna and theca externa isolat ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.