Non-specific chemical inhibition of the Fanconi anemia pathway
... However, despite important efforts, no specific inhibitor of the FA pathway has been identified so far. In the current study, using a human cell-based assay, we completed screening of more than 16,000 chemicals for molecules that inhibit the FA pathway, and identified ...
... However, despite important efforts, no specific inhibitor of the FA pathway has been identified so far. In the current study, using a human cell-based assay, we completed screening of more than 16,000 chemicals for molecules that inhibit the FA pathway, and identified ...
Expression of the papillomavirus E2 protein in HeLa cells leads to
... whether the ectopically expressed E2 protein was able to repress endogenous E6/E7 transcription in HeLa cells. Our main concern was that the HPV18 regulatory region integrated into the genomic DNA could present a chromatin structure different from that adopted on a transiently transfected plasmid, l ...
... whether the ectopically expressed E2 protein was able to repress endogenous E6/E7 transcription in HeLa cells. Our main concern was that the HPV18 regulatory region integrated into the genomic DNA could present a chromatin structure different from that adopted on a transiently transfected plasmid, l ...
Multiple Mechanisms of Resistance to Polyglutamatable and
... used as an indicator of an apparent increase in the number of lysosomes, using a method modified from Hall et al. (1978). Cells were washed twice in homogenization buffer (1 mM EDTA, 250 mM sucrose, pH 7.2), and lysates were prepared by homogenization on ice using a 5-ml Dounce homogenizer, until 70 ...
... used as an indicator of an apparent increase in the number of lysosomes, using a method modified from Hall et al. (1978). Cells were washed twice in homogenization buffer (1 mM EDTA, 250 mM sucrose, pH 7.2), and lysates were prepared by homogenization on ice using a 5-ml Dounce homogenizer, until 70 ...
Dynamin 2 mediates fluid-phase micropinocytosis in epithelial cells
... material Fig. S2B⬘), treatment with either the pooled mixture of Dyn2-siRNA duplexes or Dyn2-siRNA duplex #2 reduced Dyn2 protein levels as well as transferrin uptake by ~70-80% (supplementary material Fig. S2A,C,D). In support of the microinjection experiments shown in Fig. 1, cells treated with Dy ...
... material Fig. S2B⬘), treatment with either the pooled mixture of Dyn2-siRNA duplexes or Dyn2-siRNA duplex #2 reduced Dyn2 protein levels as well as transferrin uptake by ~70-80% (supplementary material Fig. S2A,C,D). In support of the microinjection experiments shown in Fig. 1, cells treated with Dy ...
pH and Cancer: Acidic pH Levels Can Lead To Cancer
... These abnormal cells are called malignant cells. Malignant cells do not correspond with brain function nor with our own DNA memory code. Therefore, malignant cells grow indefinitely and without order. This is cancer. As you can see, he is describing, from a different point of view, the link between ...
... These abnormal cells are called malignant cells. Malignant cells do not correspond with brain function nor with our own DNA memory code. Therefore, malignant cells grow indefinitely and without order. This is cancer. As you can see, he is describing, from a different point of view, the link between ...
Protein sensing in living cells by molecular rotor
... compounds. Although the CCVJ dye exhibits a strong uorescence signal in a highly viscous environment, the results from the two negative control compounds indicate that an intracellular environment might not be viscous enough to generate a strong uorescence signal, as in the case of the reaction be ...
... compounds. Although the CCVJ dye exhibits a strong uorescence signal in a highly viscous environment, the results from the two negative control compounds indicate that an intracellular environment might not be viscous enough to generate a strong uorescence signal, as in the case of the reaction be ...
Pap and HPV Testing
... frequent screening or to continue screening beyond age 65. These risk factors include being infected with the human immunodeficiency virus (HIV), being immunosuppressed, having been exposed to diethylstilbestrol before birth, and having been treated for a precancerous cervical lesion or cervical can ...
... frequent screening or to continue screening beyond age 65. These risk factors include being infected with the human immunodeficiency virus (HIV), being immunosuppressed, having been exposed to diethylstilbestrol before birth, and having been treated for a precancerous cervical lesion or cervical can ...
Designing cell lines for viral vaccine production: where do we stand?
... and veterinary vaccines. Insect cell lines that are typically used for recombinant protein production or VLPs are not in the scope of this review. Throughout, new options that become available with such designed cell lines are highlighted. 2 Established cell lines used for vaccine production Many of ...
... and veterinary vaccines. Insect cell lines that are typically used for recombinant protein production or VLPs are not in the scope of this review. Throughout, new options that become available with such designed cell lines are highlighted. 2 Established cell lines used for vaccine production Many of ...
Unique Eomes+ NK Cell Subsets Are Present in Uterus
... Decidual and uterine natural killer (NK) cells have been shown to contribute to the successful pregnancy both in humans and mice. NK cells represent “cytotoxic” group 1 innate lymphoid cells (ILCs) and are distinct from the recently described “helper” ILC1. Here, we show that both in humans and mice ...
... Decidual and uterine natural killer (NK) cells have been shown to contribute to the successful pregnancy both in humans and mice. NK cells represent “cytotoxic” group 1 innate lymphoid cells (ILCs) and are distinct from the recently described “helper” ILC1. Here, we show that both in humans and mice ...
Phosphoinositide Signaling Regulates the Exocyst Complex and
... fixation and immunostaining for PIPKIgi2 (green) and Rab4 or Rab11 (red). Boxes are selected regions for magnified view. (C) Cell migration enhances a PIPKIgi2 association with b1-integrin and talin. Confluent MDA-MB-231 or HeLa cells were wounded extensively so that about 50% of cells were detached ...
... fixation and immunostaining for PIPKIgi2 (green) and Rab4 or Rab11 (red). Boxes are selected regions for magnified view. (C) Cell migration enhances a PIPKIgi2 association with b1-integrin and talin. Confluent MDA-MB-231 or HeLa cells were wounded extensively so that about 50% of cells were detached ...
Isolation of Chinese Hamster Ovary Cell Mutants Requiring the
... that 0.3 ug/ml of taxol was used as the selecting drug concentration. Surviving colonies, which arose at a frequency of 10-5, were picked into taxol-containing medium, grown, recloned, and tested for growth in the presence or absence of the drug. DNA and Protein Synthesis: The ability of the mutant ...
... that 0.3 ug/ml of taxol was used as the selecting drug concentration. Surviving colonies, which arose at a frequency of 10-5, were picked into taxol-containing medium, grown, recloned, and tested for growth in the presence or absence of the drug. DNA and Protein Synthesis: The ability of the mutant ...
Mariam SH et al BMC 2017 - Armauer Hansen Research Institute
... 0.4 μm and pore density of 1 × 108 pores/cm2) were used. The Transwell has an upper and a lower chamber, with volume capacities of 9 mL and 13 mL respectively. The upper chamber has three slits for pipetting access to the lower chamber. For L. monocytogenes, two types of cultures were set-up: (i) co ...
... 0.4 μm and pore density of 1 × 108 pores/cm2) were used. The Transwell has an upper and a lower chamber, with volume capacities of 9 mL and 13 mL respectively. The upper chamber has three slits for pipetting access to the lower chamber. For L. monocytogenes, two types of cultures were set-up: (i) co ...
Memoryless self-reinforcing directionality in endosomal active
... actual turns, we defined ‘flights’, which are consecutive runs that persist in direction punctuated by pauses (Fig. 2c), and identified the turning points between flights, employing to do so the errorradius analysis standard in the field of ecology7 (Methods and Supplementary Figs 2 and 3). We find ...
... actual turns, we defined ‘flights’, which are consecutive runs that persist in direction punctuated by pauses (Fig. 2c), and identified the turning points between flights, employing to do so the errorradius analysis standard in the field of ecology7 (Methods and Supplementary Figs 2 and 3). We find ...
Lecture 19. secondary phloem-2
... are virtually impossible to identify in transverse section – the sieve areas cannot be seen in this micrograph. Albuminous cells occur in the rays; they are not sister cells to the sieve cells and are not even produced by the fusiform initials. Much of the outer part of the phloem rows are undulate: ...
... are virtually impossible to identify in transverse section – the sieve areas cannot be seen in this micrograph. Albuminous cells occur in the rays; they are not sister cells to the sieve cells and are not even produced by the fusiform initials. Much of the outer part of the phloem rows are undulate: ...
Mechanism of Inhibition of Cell Proliferation by
... \Ve have used a structure-activity approach to investigate whether the Vinca alkaloids inhibit cell proliferation primarily by means of their effects on mitotic spindle microtubules or by another mechanism or by a combination of mechanisms. Five Vìnca alkaloids were used to investigate the relation ...
... \Ve have used a structure-activity approach to investigate whether the Vinca alkaloids inhibit cell proliferation primarily by means of their effects on mitotic spindle microtubules or by another mechanism or by a combination of mechanisms. Five Vìnca alkaloids were used to investigate the relation ...
Syrosingopine sensitizes cancer cells to killing by metformin
... liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug that can be taken for years with few adverse effects, such as rare cases of lactic acidosis. There is increasing evidence that metformin, in ...
... liver and muscle, results in reduced blood glucose levels and restoration of insulin responsiveness at the whole-body level. Metformin is a well-tolerated drug that can be taken for years with few adverse effects, such as rare cases of lactic acidosis. There is increasing evidence that metformin, in ...
Discreteness of chromosome territories
... is whether chromosomes are interwoven with one another at their periphery or whether they form mutually exclusive territories. Similarly, we might ask to what extent subdomains within chromosomes are interwoven. Some data related to this topic have been published previously. Images of interphase nuc ...
... is whether chromosomes are interwoven with one another at their periphery or whether they form mutually exclusive territories. Similarly, we might ask to what extent subdomains within chromosomes are interwoven. Some data related to this topic have been published previously. Images of interphase nuc ...
Lamin proteins form an internal nucleoskeleton as well as a
... sibility can be increased by removing chromatin. Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally. ...
... sibility can be increased by removing chromatin. Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally. ...
Processing of human cytomegalovirus envelope glycoproteins in
... 1988). Furthermore, by using monensin to prevent posttranslational processing in the Golgi network of SF cells, in which O-glycosylation occurs, the Mr of gp47-63 was reduced by 20000 (Gretch et al., 1988b). This decrease was similar to that observed when gp47-63 glycoproteins were synthesized in U- ...
... 1988). Furthermore, by using monensin to prevent posttranslational processing in the Golgi network of SF cells, in which O-glycosylation occurs, the Mr of gp47-63 was reduced by 20000 (Gretch et al., 1988b). This decrease was similar to that observed when gp47-63 glycoproteins were synthesized in U- ...
Staphylococcus aureus-induced G2/M phase transition delay
... selected on the basis of the preliminary experiments to ensure that the cells still proliferate at the end of the cell-bacteria coincubation period. Unbound bacteria were removed 2 h postinfection by washing the wells with PBS, followed by incubation in cDMEM-Gent100 for 2 h, and then by incubation ...
... selected on the basis of the preliminary experiments to ensure that the cells still proliferate at the end of the cell-bacteria coincubation period. Unbound bacteria were removed 2 h postinfection by washing the wells with PBS, followed by incubation in cDMEM-Gent100 for 2 h, and then by incubation ...
University of Birmingham Leptomycin B induces apoptosis in cells
... (reviewed in ref. 1). The varying ability of the different HPV types to induce either benign lesions or anogenital neoplasia has led to their classification as either high risk (e.g. HPV 16 or 18) or low risk (e.g. HPV 6 or 11) types (2). We have previously shown that the nuclear export inhibitor LM ...
... (reviewed in ref. 1). The varying ability of the different HPV types to induce either benign lesions or anogenital neoplasia has led to their classification as either high risk (e.g. HPV 16 or 18) or low risk (e.g. HPV 6 or 11) types (2). We have previously shown that the nuclear export inhibitor LM ...
The Aromatic-L-Amino Acid Decarboxylase Inhibitor Carbidopa Is
... Fig. 3 A, Western analysis of AAAD in NCI-H727 carcinoid cells. Upper numbers, the quantity of homogenate protein per sample (g). Data represent one of triplicate experiments. B, time course of carbidopa effect on NCI-H727 carcinoid cell number. Cells (50,000/plate) were incubated at 37° with carbi ...
... Fig. 3 A, Western analysis of AAAD in NCI-H727 carcinoid cells. Upper numbers, the quantity of homogenate protein per sample (g). Data represent one of triplicate experiments. B, time course of carbidopa effect on NCI-H727 carcinoid cell number. Cells (50,000/plate) were incubated at 37° with carbi ...
Role of Cytoskeleton Changes and Expression
... the presence of TPA, and are often reversible upon removal of TPA, it is not clear which of the TPA-mediated effects are essential to promote the latent, initiated cells. To examine possible interactions between a tumor promoter, phenotypic modulation, and oncogene expression during pro motion, we u ...
... the presence of TPA, and are often reversible upon removal of TPA, it is not clear which of the TPA-mediated effects are essential to promote the latent, initiated cells. To examine possible interactions between a tumor promoter, phenotypic modulation, and oncogene expression during pro motion, we u ...
CDK11p58 kinase activity is required to protect sister chromatid
... Previous studies have reported that the lack of the short isoform (p58) of CDK11 is responsible for the cohesion defect observed in CDK11 depletion experiments . Since CDK11p58 is expressed during G2 and M stages of the cell cycle, it can be involved in the establishment of cohesion in S/G2 phase, i ...
... Previous studies have reported that the lack of the short isoform (p58) of CDK11 is responsible for the cohesion defect observed in CDK11 depletion experiments . Since CDK11p58 is expressed during G2 and M stages of the cell cycle, it can be involved in the establishment of cohesion in S/G2 phase, i ...
INTERLEUKIN 6 DECREASES CELL
... carcinoma cells were obtained from the American Type Culture Collection, Rockville, MD. The T-47 line was established from the pleural effusion obtained from a female patient with an infiltrating ductal carcinoma ofthe breast (30). The differentiated epithelial subline T47D (30) contains cytoplasmic ...
... carcinoma cells were obtained from the American Type Culture Collection, Rockville, MD. The T-47 line was established from the pleural effusion obtained from a female patient with an infiltrating ductal carcinoma ofthe breast (30). The differentiated epithelial subline T47D (30) contains cytoplasmic ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.