Extended Survivability of Prostate Cancer Cells
... hormone-refractory human prostate cancer cells. Little information exists in regard to differential properties of normal and cancerous prostate epithelial cells in terms of sensitivity to apoptosis induction. Furthermore, no systematic studies have been performed to investigate the interrelationship ...
... hormone-refractory human prostate cancer cells. Little information exists in regard to differential properties of normal and cancerous prostate epithelial cells in terms of sensitivity to apoptosis induction. Furthermore, no systematic studies have been performed to investigate the interrelationship ...
Pro-Survival Role of Gelsolin in Mouse β
... We have previously shown that the Ca2ⴙ-dependent actinsevering protein gelsolin plays an important role in regulated insulin secretion. The aim of this study was to determine the role of gelsolin in -cell survival as it has been shown to play a dual role in apoptosis in other cell types. MIN6 subcl ...
... We have previously shown that the Ca2ⴙ-dependent actinsevering protein gelsolin plays an important role in regulated insulin secretion. The aim of this study was to determine the role of gelsolin in -cell survival as it has been shown to play a dual role in apoptosis in other cell types. MIN6 subcl ...
Stimulated release of fluorescently labeled IgE fragments that
... 5 ml of 2×107 RBL-2H3 cells/ml that had been sensitized with FITCIgE overnight were suspended in BSS and stimulated with 500 ng/ml DNP-BSA in the presence of 3 µM cytochalasin D for 1 hour at 37°C. The cells were maintained in suspension by continual rotation, and an identical cell sample with 3 µM ...
... 5 ml of 2×107 RBL-2H3 cells/ml that had been sensitized with FITCIgE overnight were suspended in BSS and stimulated with 500 ng/ml DNP-BSA in the presence of 3 µM cytochalasin D for 1 hour at 37°C. The cells were maintained in suspension by continual rotation, and an identical cell sample with 3 µM ...
Inhibition of L-Fucose Incorporation into
... form (17, 18). Although the available evidence favors the incorporation of 6-TG into DNA as being critical to the cytotoxic action of this drug (26), the mechanism by which the incorporated analog exerts its cytotoxic effects has not been delineated (22), and an exception to the importance of this l ...
... form (17, 18). Although the available evidence favors the incorporation of 6-TG into DNA as being critical to the cytotoxic action of this drug (26), the mechanism by which the incorporated analog exerts its cytotoxic effects has not been delineated (22), and an exception to the importance of this l ...
ChChd3, an Inner Mitochondrial Membrane Protein, Is
... Furthermore, ChChd3 was found to be significantly downregulated in mitochondrial proteomic analysis of a cell line model of familial amyotrophic lateral sclerosis expressing SOD1 mutant G93A compared with the control cells expressing WT SOD1 (17), suggesting its possible role in the pathophysiology ...
... Furthermore, ChChd3 was found to be significantly downregulated in mitochondrial proteomic analysis of a cell line model of familial amyotrophic lateral sclerosis expressing SOD1 mutant G93A compared with the control cells expressing WT SOD1 (17), suggesting its possible role in the pathophysiology ...
Induction of the Stem-like Cell Regulator CD44
... under spheroid culture conditions (5). In an attempt to establish the optimum conditions for the maintenance and growth of cancer-initiating cells, we examined the effects on spheroid formation of chemicals that were reported to be effective in promoting the growth of normal or cancer stem cells (da ...
... under spheroid culture conditions (5). In an attempt to establish the optimum conditions for the maintenance and growth of cancer-initiating cells, we examined the effects on spheroid formation of chemicals that were reported to be effective in promoting the growth of normal or cancer stem cells (da ...
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... capacity for disease treatment. To do this, isolating exosome from cells that originate from patients themselves to avoid immuno-reaction caused by foreign substances. In this context, adipose tissue derived mesenchymal stem cells are ideal source for collecting exosome. These exosome can be used no ...
... capacity for disease treatment. To do this, isolating exosome from cells that originate from patients themselves to avoid immuno-reaction caused by foreign substances. In this context, adipose tissue derived mesenchymal stem cells are ideal source for collecting exosome. These exosome can be used no ...
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... protrusive activity was measured from low-light recordings by counting new protrusions per unit time. A new protrusion is one forming in a new position, in a new direction, or renewed advance of a previously inactive protrusion. To describe the direction of protrusions, they were plotted as falling ...
... protrusive activity was measured from low-light recordings by counting new protrusions per unit time. A new protrusion is one forming in a new position, in a new direction, or renewed advance of a previously inactive protrusion. To describe the direction of protrusions, they were plotted as falling ...
Cephalostatin 1 Selectively Triggers the Release
... were cleared by centrifugation, and protein concentration was determined. Anti-Smac antibody and anti-caspase-9 antibody (2.5 l), respectively, were added to 250 g of protein (1 g/l) and mixed overnight at 4°C. Washed protein A-agarose beads (50 l) were added for an additional 2–3 h and subsequ ...
... were cleared by centrifugation, and protein concentration was determined. Anti-Smac antibody and anti-caspase-9 antibody (2.5 l), respectively, were added to 250 g of protein (1 g/l) and mixed overnight at 4°C. Washed protein A-agarose beads (50 l) were added for an additional 2–3 h and subsequ ...
The AMF-R tubule is a smooth ilimaquinone
... Sigma. Monoclonal antibody against AMF-R was used in the form of concentrated hybridoma supernatant (Nabi et al., 1990). Antibody against ERGIC-53 was kindly provided by Hans-Peter Hauri (Biozentrum, Basel, Switzerland). Antibodies to Tac (IL25) were purchased from AMAC (Westbrook, ME). Antibodies t ...
... Sigma. Monoclonal antibody against AMF-R was used in the form of concentrated hybridoma supernatant (Nabi et al., 1990). Antibody against ERGIC-53 was kindly provided by Hans-Peter Hauri (Biozentrum, Basel, Switzerland). Antibodies to Tac (IL25) were purchased from AMAC (Westbrook, ME). Antibodies t ...
Pseudolaric Acid B Induced Cell Cycle Arrest, Au
... kaempferi Gordon (Pinaceae), known as “Tu-Jin-Pi” which had been used to treat dermatological fungi http://www.medsci.org ...
... kaempferi Gordon (Pinaceae), known as “Tu-Jin-Pi” which had been used to treat dermatological fungi http://www.medsci.org ...
reviews - London Health Sciences Centre
... It has long been recognized that some types of cancer show an organ-specific pattern of metastasis. Breast cancer frequently metastasizes to bone, liver, brain and lungs; prostate cancer preferentially spreads to bone. Patients with colorectal cancer, by contrast, often develop initial metastases in ...
... It has long been recognized that some types of cancer show an organ-specific pattern of metastasis. Breast cancer frequently metastasizes to bone, liver, brain and lungs; prostate cancer preferentially spreads to bone. Patients with colorectal cancer, by contrast, often develop initial metastases in ...
LATS1/WARTS phosphorylates MYPT1 to counteract PLK1 and
... This suggests that LATS1 promotes MYPT1 to antagonize PLK1 activity. Consistent with this, LATS1-depleted HeLa cells or fibroblasts from LATS1 knockout mice showed in creased PLK1 activity. We also found deoxyribonucleic acid (DNA) damage–induced LATS1 activation caused PLK1 suppression via the ph ...
... This suggests that LATS1 promotes MYPT1 to antagonize PLK1 activity. Consistent with this, LATS1-depleted HeLa cells or fibroblasts from LATS1 knockout mice showed in creased PLK1 activity. We also found deoxyribonucleic acid (DNA) damage–induced LATS1 activation caused PLK1 suppression via the ph ...
Mitotic Block Induced in HeLa Cells by Low Concentrations of
... cells had exited mitosis into an apparent interphase state (as deter mined by decondensation of chromosomes and reformation of nuclear membrane, described further below and in Fig. 5). For example, 24 h after 10 nM paclitaxel was removed from the medium, the percentage of cells in mitosis was reduce ...
... cells had exited mitosis into an apparent interphase state (as deter mined by decondensation of chromosomes and reformation of nuclear membrane, described further below and in Fig. 5). For example, 24 h after 10 nM paclitaxel was removed from the medium, the percentage of cells in mitosis was reduce ...
T Cell Proliferation Induced by Autologous Non
... associated with augmented induction of autologous T cell proliferation. Our data show that caspase activity in the non-T stimulator population is essential for induction of autologous T cell proliferation, suggesting that cellular components in the non-T cell fraction are enzymatically modified, mos ...
... associated with augmented induction of autologous T cell proliferation. Our data show that caspase activity in the non-T stimulator population is essential for induction of autologous T cell proliferation, suggesting that cellular components in the non-T cell fraction are enzymatically modified, mos ...
Minimal Time Requirement for Lasting Elicitor Effects in
... bited uptake of both inorganic phosphate and amino acids by cultured parsley cells. The inhibition of phosphate uptake was completely reversed if the elicitor was removed within a time period of approximately 20 min following its addition to the cell cultures. Cells which had been exposed to the eli ...
... bited uptake of both inorganic phosphate and amino acids by cultured parsley cells. The inhibition of phosphate uptake was completely reversed if the elicitor was removed within a time period of approximately 20 min following its addition to the cell cultures. Cells which had been exposed to the eli ...
Structural Requirements of Simple Organic
... relationship between lipophilicity and recognition by P-glycoprotein-mediated MDR. In addition, since the guanidiniums each of the compounds tested are presented in Table 1. It is did not contain an aromatic moiety but the pyridiniums did, we apparent that for alkylGu"1" and alkylPy"1", toxicity in ...
... relationship between lipophilicity and recognition by P-glycoprotein-mediated MDR. In addition, since the guanidiniums each of the compounds tested are presented in Table 1. It is did not contain an aromatic moiety but the pyridiniums did, we apparent that for alkylGu"1" and alkylPy"1", toxicity in ...
Histochemical Polymorphism of Keratin Pearls in Squamous Cell
... It can be assumed that such staining of PAS-positive keratin pearls is due to development of parakeratosis, i.e. the process of keratinization abnormality. In conventional histological hematoxylin and eosin staining the third type of keratin pearls is revealed, which are characterized with weak eosi ...
... It can be assumed that such staining of PAS-positive keratin pearls is due to development of parakeratosis, i.e. the process of keratinization abnormality. In conventional histological hematoxylin and eosin staining the third type of keratin pearls is revealed, which are characterized with weak eosi ...
RNA-binding Proteins TIA-1 and TIAR Link the
... HE general translational arrest that accompanies environmental stress is initiated by the phosphorylation of eIF-2a, an essential component of the heterotrimeric eIF-2 complex that loads the initiator tRNA (Met-tRNAMet) onto the 40S ribosomal subunit (Berlanga et al., 1998; Gray and Wickens, 1998; S ...
... HE general translational arrest that accompanies environmental stress is initiated by the phosphorylation of eIF-2a, an essential component of the heterotrimeric eIF-2 complex that loads the initiator tRNA (Met-tRNAMet) onto the 40S ribosomal subunit (Berlanga et al., 1998; Gray and Wickens, 1998; S ...
Atomic Force Microscopy in Cancer Cell Research
... ideally flat, then it would be irrelevant at what point to measure forces. In the case of biological cells, knowledge of geometry is important because cells are not necessarily flat. Therefore, one will need some kind of automatic collection of data on forces and the geometry of the surface at the sam ...
... ideally flat, then it would be irrelevant at what point to measure forces. In the case of biological cells, knowledge of geometry is important because cells are not necessarily flat. Therefore, one will need some kind of automatic collection of data on forces and the geometry of the surface at the sam ...
Comparison of an immortalized human corneal epithelial cell line
... Susceptibility of HCE to HSV-1 and its suitability for the isolation of HSV-1 The HCE was found to be suceptible to HSV-1 as determined by both the shell vial and tube cultures. Cytopathic effect (CPE) (fig. 2) was seen in the tube cultures at 48 h (>50% of the cells were refractile, showed rounding ...
... Susceptibility of HCE to HSV-1 and its suitability for the isolation of HSV-1 The HCE was found to be suceptible to HSV-1 as determined by both the shell vial and tube cultures. Cytopathic effect (CPE) (fig. 2) was seen in the tube cultures at 48 h (>50% of the cells were refractile, showed rounding ...
All-Trans Retinoic Acid Induces DU145 Cell Cycle Arrest through
... DU145 cell line (BCRC 60348), an androgen-independent prostate cancer cell line, was obtained from the Culture Collection and Research Center, Food Industry Research and Development Institute, Taiwan, Republic of China. DU145 cells were cultured in DMEM medium (Sigma Co., St. Louis, MO) plus 10% fet ...
... DU145 cell line (BCRC 60348), an androgen-independent prostate cancer cell line, was obtained from the Culture Collection and Research Center, Food Industry Research and Development Institute, Taiwan, Republic of China. DU145 cells were cultured in DMEM medium (Sigma Co., St. Louis, MO) plus 10% fet ...
Human skin cells support thymus- independent T cell development
... because the unique requirements for T cell development are difficult to replicate in vitro. T cell differentiation involves an obligate period of development and education in the thymus, an organ that undergoes involution in late adolescence and maintains only a low level of activity in normal adult ...
... because the unique requirements for T cell development are difficult to replicate in vitro. T cell differentiation involves an obligate period of development and education in the thymus, an organ that undergoes involution in late adolescence and maintains only a low level of activity in normal adult ...
On the mechanisms of nectar secretion: revisited
... endoplasmic reticulum or Golgi bodies ( previously dictyosomes). They then supposedly produce vesicles with nectar that are released through fusion with the plasma membrane. The presence of a well-developed endoplasmic reticulum, numerous Golgi bodies and vesicles is considered to be the distinctive ...
... endoplasmic reticulum or Golgi bodies ( previously dictyosomes). They then supposedly produce vesicles with nectar that are released through fusion with the plasma membrane. The presence of a well-developed endoplasmic reticulum, numerous Golgi bodies and vesicles is considered to be the distinctive ...
Stem cells in cancer: instigators and propagators?
... clones until an additional mutation produces the malignant cell phenotype that can invade beyond normal tissue boundaries and, subsequently, spread to distant sites – a phenomenon known as metastasis. We now believe that most tumours contain a subpopulation of malignant cells with stem cell properti ...
... clones until an additional mutation produces the malignant cell phenotype that can invade beyond normal tissue boundaries and, subsequently, spread to distant sites – a phenomenon known as metastasis. We now believe that most tumours contain a subpopulation of malignant cells with stem cell properti ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.