Biological Properties of Tinospora crispa (Akar Patawali) and Its
... 79.3%, stem = 77.9%; fibre: leaves = 1.59%, stem = 0.65%; and energy: leaves = 1.59%, stem = 0.65%. The antioxidant activity of the extract prepared at various temperatures and incubation time was evaluated to determine the optimum extraction procedure. Based on DPPH and TBA tests, the preparation o ...
... 79.3%, stem = 77.9%; fibre: leaves = 1.59%, stem = 0.65%; and energy: leaves = 1.59%, stem = 0.65%. The antioxidant activity of the extract prepared at various temperatures and incubation time was evaluated to determine the optimum extraction procedure. Based on DPPH and TBA tests, the preparation o ...
Original Article Intracellular zinc distribution in mitochondria, ER and
... size, some resembled squiggly lines, while others were dot-like, reflects the morphological heterogeneity of mitochondria. As expected, mitochondria were highly co-localized with intracellular free zinc in the axon. Both the HeLa cells and neuronal axon mitochondria showed co-localization with free ...
... size, some resembled squiggly lines, while others were dot-like, reflects the morphological heterogeneity of mitochondria. As expected, mitochondria were highly co-localized with intracellular free zinc in the axon. Both the HeLa cells and neuronal axon mitochondria showed co-localization with free ...
COMMUNICATION Redefining the phenotype of Heat shock protein 90
... close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10,000 papers have been published reporting studies on Hsp90 (Scopus), of which over 5,000 use classical inhibitor ...
... close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10,000 papers have been published reporting studies on Hsp90 (Scopus), of which over 5,000 use classical inhibitor ...
(Hsp90) inhibitors
... close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10,000 papers have been published reporting studies on Hsp90 (Scopus), of which over 5,000 use classical inhibitor ...
... close analysis of these data suggest that the molecules do not target Hsp90 alone but rather have non-specific binding affinities for multiple proteins[5c, 6]. In the past 10 years over 10,000 papers have been published reporting studies on Hsp90 (Scopus), of which over 5,000 use classical inhibitor ...
the far c-terminus of tpx2 contributes to spindle morphogenesis
... Deoxyribonucleic acid (DNA) is the universal blueprint for life. It contains the genetic code that each cell uses to generate the proteome. As life evolved on Earth, organisms developed methods to ensure the delivery of replicated DNA to their progeny. Prokaryotes undergo binary fission – a process ...
... Deoxyribonucleic acid (DNA) is the universal blueprint for life. It contains the genetic code that each cell uses to generate the proteome. As life evolved on Earth, organisms developed methods to ensure the delivery of replicated DNA to their progeny. Prokaryotes undergo binary fission – a process ...
2 - Liu Lab
... hairpin RNAs produced even more pronounced effects, leading to complete tumour regression and failure to regrow even after discontinuing doxycycline treatment (Fig. 1c and Extended Data Fig. 2g). Evidence of BBI-induced basal-to-luminal differentiation was confirmed in vivo (Extended Data Fig. 2f, h ...
... hairpin RNAs produced even more pronounced effects, leading to complete tumour regression and failure to regrow even after discontinuing doxycycline treatment (Fig. 1c and Extended Data Fig. 2g). Evidence of BBI-induced basal-to-luminal differentiation was confirmed in vivo (Extended Data Fig. 2f, h ...
In Vivo Release of Mitotic Silencing of Ribosomal Gene
... very sensitive to roscovitine (IC50 , 7 mM) when tested in vitro. However, because cdk2–cyclin E and cdk2–cyclin A are active at the G1/S transition and during S phase, respectively, the cdc2–cyclin B kinase is the only known kinase inhibited by roscovitine when mitotically synchronized cells are tr ...
... very sensitive to roscovitine (IC50 , 7 mM) when tested in vitro. However, because cdk2–cyclin E and cdk2–cyclin A are active at the G1/S transition and during S phase, respectively, the cdc2–cyclin B kinase is the only known kinase inhibited by roscovitine when mitotically synchronized cells are tr ...
PDF
... naïve CD44low CD8+ T cells were purified from WT and Cblb−/− mice and labeled with CFSE. These populations were then stimulated in vitro with plate-bound anti-CD3 ab and soluble anti-CD28 ab in the presence of either plate-bound PD-L1 Ig or plate-bound control human IgG1 (control Ig). After 3 days, ...
... naïve CD44low CD8+ T cells were purified from WT and Cblb−/− mice and labeled with CFSE. These populations were then stimulated in vitro with plate-bound anti-CD3 ab and soluble anti-CD28 ab in the presence of either plate-bound PD-L1 Ig or plate-bound control human IgG1 (control Ig). After 3 days, ...
Resident in Normal Skin T Cells Are + The Vast Majority of CLA
... method involves culture of skin explants on three-dimensional matrices that induce the ingrowth of dermal fibroblasts. Chemokines produced by these fibroblasts induce the migration of T cells out of explants, where they can be collected and studied. Although this method requires a culture period, it ...
... method involves culture of skin explants on three-dimensional matrices that induce the ingrowth of dermal fibroblasts. Chemokines produced by these fibroblasts induce the migration of T cells out of explants, where they can be collected and studied. Although this method requires a culture period, it ...
Proteins that contain a functional Z-DNA
... in their localization to stress granules. We additionally show that the Za domain from other Z-DNAbinding proteins (ZBP1, E3L) is likewise sufficient for localization to stress granules. Finally, we show that Z-RNA or Z-DNA binding is important for stress granule localization. We have thus identifie ...
... in their localization to stress granules. We additionally show that the Za domain from other Z-DNAbinding proteins (ZBP1, E3L) is likewise sufficient for localization to stress granules. Finally, we show that Z-RNA or Z-DNA binding is important for stress granule localization. We have thus identifie ...
Mixed Lineage Kinase Domain-like Protein Mediates Necrosis
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
... The importance of necrotic cell death has been illustrated by several recent findings. Breeding onto a RIP3 knockout background rescues the developmental defects of caspase-8 knockout mice. This indicates that the cardiac, vascular, and hematopoietic defects that occur during the development of casp ...
Cell cycle behavior of human HP1 subtypes: distinct
... Intracellular localization of YFP-fused HP1 subtypes We examined the localization of YFP-fusion constructs of human HP1 subtypes in HeLa cells. HeLa cells transiently expressing YFP-fused HP1 were fixed and observed under a fluorescence microscope. In interphase nuclei, YFP-fused HP1 showed two diff ...
... Intracellular localization of YFP-fused HP1 subtypes We examined the localization of YFP-fusion constructs of human HP1 subtypes in HeLa cells. HeLa cells transiently expressing YFP-fused HP1 were fixed and observed under a fluorescence microscope. In interphase nuclei, YFP-fused HP1 showed two diff ...
Active Uptake and Extravesicular Storage of m
... radiopharmaceutical in this tumor category. The present study demonstrates for the first time extensive extravesicular storage of MIBG in a single in vitro model of human neuroblastoma. Obviously, nongranular storage will have an important, al though as yet unknown, impact on the biological half-lif ...
... radiopharmaceutical in this tumor category. The present study demonstrates for the first time extensive extravesicular storage of MIBG in a single in vitro model of human neuroblastoma. Obviously, nongranular storage will have an important, al though as yet unknown, impact on the biological half-lif ...
Role for Rab7 in maturation of late autophagic vacuoles
... lysosomal enzyme inhibitors. The amount of the membraneassociated, 16 kDa LC3II increased at the same rate as the volume fraction of autophagic vacuoles in HeLa cells, while the amount of the soluble, 18 kDa LC3I decreased (Kabeya et al., 2000). It was proposed that during autophagy LC3I was process ...
... lysosomal enzyme inhibitors. The amount of the membraneassociated, 16 kDa LC3II increased at the same rate as the volume fraction of autophagic vacuoles in HeLa cells, while the amount of the soluble, 18 kDa LC3I decreased (Kabeya et al., 2000). It was proposed that during autophagy LC3I was process ...
GSK-3 Promotes Cell Survival, Growth, and PAX3 Levels in Human
... tissues (2–4, 8). Gsk-3a and Gsk-3b null mice further highlight these different functions, as loss of either gene results in very different phenotypes (9–12). Deregulation of GSK-3 expression and activity is implicated in the growth and survival of melanoma, but the role of the individual isoforms i ...
... tissues (2–4, 8). Gsk-3a and Gsk-3b null mice further highlight these different functions, as loss of either gene results in very different phenotypes (9–12). Deregulation of GSK-3 expression and activity is implicated in the growth and survival of melanoma, but the role of the individual isoforms i ...
Full-Text PDF
... target them to the lysosomal pathway; the degradation of pathogens through the autophagy pathway is known as xenophagy [2]. However, most infectious pathogens have evolved strategies to escape autophagy or even to use some properties of this cellular mechanism to optimize their intracellular life cy ...
... target them to the lysosomal pathway; the degradation of pathogens through the autophagy pathway is known as xenophagy [2]. However, most infectious pathogens have evolved strategies to escape autophagy or even to use some properties of this cellular mechanism to optimize their intracellular life cy ...
Targeting of Salmonella typhimurium to Vesicles Containing
... specific function has been assigned to these glycosylated proteins, although they may function in protecting lysosomal membranes from hydrolytic enzymes present in the lumen of this organelle. Recent studies have also shown that lgps are overexpressed on the cell surface of activated platelets (Febb ...
... specific function has been assigned to these glycosylated proteins, although they may function in protecting lysosomal membranes from hydrolytic enzymes present in the lumen of this organelle. Recent studies have also shown that lgps are overexpressed on the cell surface of activated platelets (Febb ...
Max and inhibitory c-Myc mutants induce erythroid di
... We have used the human leukemia cell line K562 as a model to study the role of c-myc in dierentiation and apoptosis. We have generated stable transfectants of K562 constitutively expressing two c-Myc inhibitory mutants: D106-143, that carries a deletion in the transactivation domain of the protein, ...
... We have used the human leukemia cell line K562 as a model to study the role of c-myc in dierentiation and apoptosis. We have generated stable transfectants of K562 constitutively expressing two c-Myc inhibitory mutants: D106-143, that carries a deletion in the transactivation domain of the protein, ...
Full Text - The International Journal of Developmental Biology
... 2014, this volume). The plane of third cleavage is oblique to the in most species, the 4d cell is the sole source of another type of animal-vegetal axis; in each quadrant, a quartet of micromeres is mesoderm, the so-called endomesoderm (Lambert, 2008; Hejnol, born at the animal pole, and situated in ...
... 2014, this volume). The plane of third cleavage is oblique to the in most species, the 4d cell is the sole source of another type of animal-vegetal axis; in each quadrant, a quartet of micromeres is mesoderm, the so-called endomesoderm (Lambert, 2008; Hejnol, born at the animal pole, and situated in ...
ER storage diseases: a role for ERGIC
... absence of L chain in the inducible H[⌬CH1] clone The co-expression of mutant and chains dramatically influenced the shape of RB structures in non-lymphoid cells also (Fig. 4). Instead of the irregularly shaped structures concentrated around the MTOC area (Fig. 4a), numerous spherical vacuoles, ...
... absence of L chain in the inducible H[⌬CH1] clone The co-expression of mutant and chains dramatically influenced the shape of RB structures in non-lymphoid cells also (Fig. 4). Instead of the irregularly shaped structures concentrated around the MTOC area (Fig. 4a), numerous spherical vacuoles, ...
DAZL is essential for stress granule formation
... components are overexpressed (Anderson and Kedersha, 2008). The mRNPs of SGs are in a dynamic equilibrium with polysomes (Kedersha et al., 1999; Kedersha et al., 2000). In this regard, SGs have been proposed to function as a transient place of mRNP remodeling for storage, degradation or reinitiation ...
... components are overexpressed (Anderson and Kedersha, 2008). The mRNPs of SGs are in a dynamic equilibrium with polysomes (Kedersha et al., 1999; Kedersha et al., 2000). In this regard, SGs have been proposed to function as a transient place of mRNP remodeling for storage, degradation or reinitiation ...
The GARP complex is required for cellular sphingolipid homeostasis
... normally. The levels of sphingolipids in the membrane have to be tightly controlled because any imbalance can cause stress to the cell and can lead to serious diseases. Sphingolipids are made inside the cell and are then sent to a compartment called the Golgi before being delivered to the membrane. ...
... normally. The levels of sphingolipids in the membrane have to be tightly controlled because any imbalance can cause stress to the cell and can lead to serious diseases. Sphingolipids are made inside the cell and are then sent to a compartment called the Golgi before being delivered to the membrane. ...
mycoplasmas in tissue culture
... Mycoplasma contamination of tissue cultures has been recognized for almost 10 years, but it is only comparatively recently that an adequate understanding has been gained of the source of the contaminants and the reasons for their wide dissemination in cultures. There have also been many recent obser ...
... Mycoplasma contamination of tissue cultures has been recognized for almost 10 years, but it is only comparatively recently that an adequate understanding has been gained of the source of the contaminants and the reasons for their wide dissemination in cultures. There have also been many recent obser ...
CD133 CXCR4 colon cancer cells exhibit metastatic potential and predict poor prognosis
... Background: Colorectal cancer (CRC), which frequently metastasizes to the liver, is one of the three leading causes of cancer-related deaths worldwide. Growing evidence suggests that a subset of cells exists among cancer stem cells. This distinct subpopulation is thought to contribute to liver metas ...
... Background: Colorectal cancer (CRC), which frequently metastasizes to the liver, is one of the three leading causes of cancer-related deaths worldwide. Growing evidence suggests that a subset of cells exists among cancer stem cells. This distinct subpopulation is thought to contribute to liver metas ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.