Toxoplasma gondii Intracellular Parasite Perforin Trigger Rapid
... CD4⫹ and CD8⫹ T cells have been demonstrated to be important in controlling T. gondii infection (reviewed in Ref. 9). It was later shown that perforin-dependent cytotoxicity played a limited role in resistance to T. gondii infection (10). Neither Fas nor TNF-receptors were subsequently demonstrated ...
... CD4⫹ and CD8⫹ T cells have been demonstrated to be important in controlling T. gondii infection (reviewed in Ref. 9). It was later shown that perforin-dependent cytotoxicity played a limited role in resistance to T. gondii infection (10). Neither Fas nor TNF-receptors were subsequently demonstrated ...
CHOI, JOON W., EVELYN B. SHERR, AND BARRY F. SHERR
... observed: the number of NV cells doubled during the initial 6 h prior to an increase in total cell counts. These results show that some bacteria without visible nucleoids are capable of becoming NV cells, and thus have DNA in a nucleoid region not detectable with the method used here. ...
... observed: the number of NV cells doubled during the initial 6 h prior to an increase in total cell counts. These results show that some bacteria without visible nucleoids are capable of becoming NV cells, and thus have DNA in a nucleoid region not detectable with the method used here. ...
hTERT Inhibition Triggers Epstein–Barr Virus Lytic Cycle and
... and several histone deacetylase inhibitors (9–12). EBVcarrying tumor cells may also be killed by prodrugs, such as ganciclovir or radiolabeled nucleoside analogs, which are activated by EBV lytic proteins, such as the viral thymidine kinase (9, 13, 14). Notably, combined treatment with arginine buty ...
... and several histone deacetylase inhibitors (9–12). EBVcarrying tumor cells may also be killed by prodrugs, such as ganciclovir or radiolabeled nucleoside analogs, which are activated by EBV lytic proteins, such as the viral thymidine kinase (9, 13, 14). Notably, combined treatment with arginine buty ...
BCL-xL-Dependent Light Scattering by Apoptotic Cells
... size/shape for objects with wavelength-scale dimensions, and was used as a morphometric measure of cellular response. Three cell variants were treated with staurosporine (STS): nontransfected parental CSM14.1, CSM14.1 stably expressing yellow fluorescent protein (YFP) with diffuse YFP fluorescence, an ...
... size/shape for objects with wavelength-scale dimensions, and was used as a morphometric measure of cellular response. Three cell variants were treated with staurosporine (STS): nontransfected parental CSM14.1, CSM14.1 stably expressing yellow fluorescent protein (YFP) with diffuse YFP fluorescence, an ...
The relative efficiency of homology
... mitotic spindle, can cause chromosomal instability (CIN) or cell death (3,4). Thus, characterizing the factors and pathways that are important for these aspects of anaphase will provide insight into genome maintenance. Decatenation stress caused by catalytic inhibition of Topoisomerase II has reveal ...
... mitotic spindle, can cause chromosomal instability (CIN) or cell death (3,4). Thus, characterizing the factors and pathways that are important for these aspects of anaphase will provide insight into genome maintenance. Decatenation stress caused by catalytic inhibition of Topoisomerase II has reveal ...
The C-terminal end of R-Ras contains a focal adhesion targeting
... Plasmids pEXV-Rac12V and pEXV-Rac17N (gifts from A. Hall) were used to excise the corresponding Rac1 mutants to reclone them to pEGFP-C1A and pEGFP-N1 (Clontech). The human Arf6 reading frame was amplified by PCR from HeLa cDNA and cloned into pEGFP-N1 or pcDNA4/TO; the Arf6-27N and Arf6-67L mutants ...
... Plasmids pEXV-Rac12V and pEXV-Rac17N (gifts from A. Hall) were used to excise the corresponding Rac1 mutants to reclone them to pEGFP-C1A and pEGFP-N1 (Clontech). The human Arf6 reading frame was amplified by PCR from HeLa cDNA and cloned into pEGFP-N1 or pcDNA4/TO; the Arf6-27N and Arf6-67L mutants ...
L-Carnosine reduces telomere damage and shortening
... Academic Science (Beijing, China). Cells were grown in DMEM supplemented with 10% fetal bovine serum and 100 U/ml penicillin/ streptomycin sulfate at 37 C with 5% CO2 and 95% air. Serial cultivation was carried out with a split ratio of 1:4 at early and of 1:2 at later passages, respectively. At ea ...
... Academic Science (Beijing, China). Cells were grown in DMEM supplemented with 10% fetal bovine serum and 100 U/ml penicillin/ streptomycin sulfate at 37 C with 5% CO2 and 95% air. Serial cultivation was carried out with a split ratio of 1:4 at early and of 1:2 at later passages, respectively. At ea ...
REACTION OF THE GOBLET CELLS TO THE CHOLINERGIC
... of voluminous intraepithelial mucous glands were usually later encountered. 20 min after administration of 0.5 mg of acetylcholine, a significant change in the distribution of the secretory elements was recorded together with the appearance of numerous undifferentiated elements and different stages ...
... of voluminous intraepithelial mucous glands were usually later encountered. 20 min after administration of 0.5 mg of acetylcholine, a significant change in the distribution of the secretory elements was recorded together with the appearance of numerous undifferentiated elements and different stages ...
Active cell migration drives the unilateral movements
... migrating proximally and start spreading laterally before reaching the extra-embryonic ectoderm, presumably because they are obstructed by the leading cells. Cells 5 and 6, which are sisters, share a common track before they divide. They have separate tracks after division, but remain in contact wit ...
... migrating proximally and start spreading laterally before reaching the extra-embryonic ectoderm, presumably because they are obstructed by the leading cells. Cells 5 and 6, which are sisters, share a common track before they divide. They have separate tracks after division, but remain in contact wit ...
Cell Cycle-specific Effects of Tumor Necrosis
... treated with 0.88 >tg TNF per flask. Beginning from 0 time, individual cultures were harvested by trypsinization at hourly intervals up to the 12th hr. Care was taken to collect both cells that were attached to dishes (removed by trypsinization) and cells floating in the medium. The latter consisted ...
... treated with 0.88 >tg TNF per flask. Beginning from 0 time, individual cultures were harvested by trypsinization at hourly intervals up to the 12th hr. Care was taken to collect both cells that were attached to dishes (removed by trypsinization) and cells floating in the medium. The latter consisted ...
Fig. 3D - Journal of Cell Science
... immunofluorescence. We found that apically extruded Scribbleknockdown cells were always positively immunostained with anti-active caspase-3 antibody and had fragmented nuclei, indicating that they had undergone apoptosis (Fig. 3A). By contrast, when cells expressing scramble shRNA were surrounded by ...
... immunofluorescence. We found that apically extruded Scribbleknockdown cells were always positively immunostained with anti-active caspase-3 antibody and had fragmented nuclei, indicating that they had undergone apoptosis (Fig. 3A). By contrast, when cells expressing scramble shRNA were surrounded by ...
ANTIBACTERIAL ACTIVITY OF HUMAN NATURAL KILLER CELLS
... Comparison of Fresh and Pre-incubated NK Cells. The experiments described above studied NK cells that had been incubated for 16 h in HB104 media, due to the duration of cell purification procedures during the preceding day. To study the effect of 16-h incubation without additional lymphokine stimula ...
... Comparison of Fresh and Pre-incubated NK Cells. The experiments described above studied NK cells that had been incubated for 16 h in HB104 media, due to the duration of cell purification procedures during the preceding day. To study the effect of 16-h incubation without additional lymphokine stimula ...
Myeloma-Specific Multiple Peptides Able to Generate Cytotoxic T
... XBP1-US184–192 (YISPWILAV), heteroclitic XBP1-SP367–375 (YLFPQLISV), native CD138260–268 (GLVGLIFAV), and native CS1239–247 (SLFVLGLFL) peptides, which have strong HLA-A2 affinity and immunogenicity in combination, for their ability to elicit multiple myeloma antigen–specific responses. Experimental ...
... XBP1-US184–192 (YISPWILAV), heteroclitic XBP1-SP367–375 (YLFPQLISV), native CD138260–268 (GLVGLIFAV), and native CS1239–247 (SLFVLGLFL) peptides, which have strong HLA-A2 affinity and immunogenicity in combination, for their ability to elicit multiple myeloma antigen–specific responses. Experimental ...
A Dictyostelium mutant with defective aggregate size
... and the smlA knockout cells were compared to their parental line, DH1; unless noted, Ax4 and DH1 cells behaved identically. The growth rate of cells was determined following Gomer and Ammann (1996). Cell motility assays were done as described in Yuen et al. (1995). For synergy experiments, mixtures ...
... and the smlA knockout cells were compared to their parental line, DH1; unless noted, Ax4 and DH1 cells behaved identically. The growth rate of cells was determined following Gomer and Ammann (1996). Cell motility assays were done as described in Yuen et al. (1995). For synergy experiments, mixtures ...
Cancer Prevention Strategies That Address the Evolutionary
... patients through follow-up surgery. This form of therapy has three attractive characteristics. First, it should not ...
... patients through follow-up surgery. This form of therapy has three attractive characteristics. First, it should not ...
Cells in Vitro - Cancer Research
... dialyzed human serum was the same as that re cells from the glass. Cell counts showed that the ported for Medium 2 (11), except that inositol was cultures had progressed uniformly within each raised to 9 fig/ml.1 All operations were performed group, and their contents were combined. The cells in eac ...
... dialyzed human serum was the same as that re cells from the glass. Cell counts showed that the ported for Medium 2 (11), except that inositol was cultures had progressed uniformly within each raised to 9 fig/ml.1 All operations were performed group, and their contents were combined. The cells in eac ...
Anatomy, Ultrastructure and Physiology of Hornwort Stomata
... dominant life stage of homworts. The gametophyte is a simple thallus, superficially resembling that of simple thalliod liverworts. The sporophyte consists of a foot embedded in the gametophyte and a long cylindrical sporangium. A basal meristem indeterminately produces new cells throughout the growi ...
... dominant life stage of homworts. The gametophyte is a simple thallus, superficially resembling that of simple thalliod liverworts. The sporophyte consists of a foot embedded in the gametophyte and a long cylindrical sporangium. A basal meristem indeterminately produces new cells throughout the growi ...
Resveratrol Inhibits Proliferation and Survival of Epstein Barr Virus
... thereby causing deregulation of c-myc. The EBV genome is present in more than 95% of endemic Burkitt's lymphoma cases in Africa and in 10% to 20% of all sporadic cases worldwide. Moreover, about 40% to 50% of AIDS-associated Burkitt's lymphoma cases carry the viral DNA. Although in most Burkitt's ly ...
... thereby causing deregulation of c-myc. The EBV genome is present in more than 95% of endemic Burkitt's lymphoma cases in Africa and in 10% to 20% of all sporadic cases worldwide. Moreover, about 40% to 50% of AIDS-associated Burkitt's lymphoma cases carry the viral DNA. Although in most Burkitt's ly ...
The Role of Thomsen-Friedenreich Antigen in
... Dot Blot Assay. Bovine ASF, bearing multiple T antigen epitopes, was Antibodies, Chemicals, and Reagents. The monoclonal anti-T antigen antibody was developed as described (26). The TIB-166 hybridoma, producing immobilized on the nitrocellulose membrane (1 g/spot). After blocking with rat monoclona ...
... Dot Blot Assay. Bovine ASF, bearing multiple T antigen epitopes, was Antibodies, Chemicals, and Reagents. The monoclonal anti-T antigen antibody was developed as described (26). The TIB-166 hybridoma, producing immobilized on the nitrocellulose membrane (1 g/spot). After blocking with rat monoclona ...
Shape engineering vs organic modification of cellular internalization
... important parameter which has received much less attention is the morphology (shape) of the nanocarrier. In order to investigate the morphology effect on the extent of cellular internalization, two similarly sized but differently shaped rod-like and spherical mesoporous silica nanoparticles were syn ...
... important parameter which has received much less attention is the morphology (shape) of the nanocarrier. In order to investigate the morphology effect on the extent of cellular internalization, two similarly sized but differently shaped rod-like and spherical mesoporous silica nanoparticles were syn ...
Vemurafenib resistance selects for highly
... 0304-3835/© 2015 Elsevier Ireland Ltd. All rights reserved. ...
... 0304-3835/© 2015 Elsevier Ireland Ltd. All rights reserved. ...
University of Zurich - Institute of Molecular Life Sciences
... The entry of the species C HAdV-2 (Ad2) and HAdV-5 (Ad5) is best characterized. Ad2/5 bind to the coxsackie B virus-Ad receptor CAR (8), and use -integrins as secondary receptors to induce receptor-mediated endocytosis involving clathrin, clathrin adaptors and the large GTPase dynamin (31, 34, 63, ...
... The entry of the species C HAdV-2 (Ad2) and HAdV-5 (Ad5) is best characterized. Ad2/5 bind to the coxsackie B virus-Ad receptor CAR (8), and use -integrins as secondary receptors to induce receptor-mediated endocytosis involving clathrin, clathrin adaptors and the large GTPase dynamin (31, 34, 63, ...
The prime cause of cancer.
... none of these things. Cancer is the body, at the cellular level, attempting to survive by reverting to a primitive survival mechanism. Surprisingly, it’s that simple. ...
... none of these things. Cancer is the body, at the cellular level, attempting to survive by reverting to a primitive survival mechanism. Surprisingly, it’s that simple. ...
1 Breast Cancer Cell Line Development and Authentication
... Johns Hopkins University and the revolution of modern biomedical research began. These cells were grown in roller flasks in specialized medium containing serum developed by Evans and Earle et al. and continued to proliferate (Evans et al., 1951). Almost 60 years later, these cells are still prolifer ...
... Johns Hopkins University and the revolution of modern biomedical research began. These cells were grown in roller flasks in specialized medium containing serum developed by Evans and Earle et al. and continued to proliferate (Evans et al., 1951). Almost 60 years later, these cells are still prolifer ...
Microtubules do not promote mitotic slippage when the spindle
... the lower the drug concentration the faster a cell escapes mitosis (Rieder and Maiato, 2004). The reasons for this remain vague. However, work on hamster (Andreassen and Margolis, 1994) and human nontransformed (Brito and Rieder, 2006) and cancer (Jordan et al., 1992) cells reveals that accelerated ...
... the lower the drug concentration the faster a cell escapes mitosis (Rieder and Maiato, 2004). The reasons for this remain vague. However, work on hamster (Andreassen and Margolis, 1994) and human nontransformed (Brito and Rieder, 2006) and cancer (Jordan et al., 1992) cells reveals that accelerated ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.