Examining the link between chromosomal instability and aneuploidy
... cells, and these were lagging chromatids at anaphase and chromatin bridges. Similar frequencies of these mitotic errors are observed in fixed cells that did not express GFP–histone H2B (7.2 ± 1.6% [SEM], n = 400), demonstrating that the video results are representative (Fig. S1 A). Chromosomes in th ...
... cells, and these were lagging chromatids at anaphase and chromatin bridges. Similar frequencies of these mitotic errors are observed in fixed cells that did not express GFP–histone H2B (7.2 ± 1.6% [SEM], n = 400), demonstrating that the video results are representative (Fig. S1 A). Chromosomes in th ...
Acta Medica Okayama
... e reaction between tumor cells and the lymphocytes of the host. However, as they all used homologous lymphocytes and antigenic cells, their experim ents are in reality executed homotransplantation immunity in vitro. In the present experiment regional lymph-node cells obtained from C3H mice sensitize ...
... e reaction between tumor cells and the lymphocytes of the host. However, as they all used homologous lymphocytes and antigenic cells, their experim ents are in reality executed homotransplantation immunity in vitro. In the present experiment regional lymph-node cells obtained from C3H mice sensitize ...
In vitro development of inner cell masses isolated immunosurgically
... the polar trophectoderm appeared to be a monolayer of cells. In vitro culture of inner cell masses isolated from blastocysts collected between 13.00 and 18.00 h on the 4th day of pregnancy Blastocysts collected at different times between 13.00 and 18.00 h (approx. 3-5-3-75 days^.c.) were divided int ...
... the polar trophectoderm appeared to be a monolayer of cells. In vitro culture of inner cell masses isolated from blastocysts collected between 13.00 and 18.00 h on the 4th day of pregnancy Blastocysts collected at different times between 13.00 and 18.00 h (approx. 3-5-3-75 days^.c.) were divided int ...
Pap Testing (What is it and why do I need it?)
... the test. In general, health care providers recommend beginning Pap testing at age 21 and then every three years if no abnormal cells are found. After age 30, Pap test are generally recommended every three years, or every five years when the Pap test is combined with an HPV test. If you have certain ...
... the test. In general, health care providers recommend beginning Pap testing at age 21 and then every three years if no abnormal cells are found. After age 30, Pap test are generally recommended every three years, or every five years when the Pap test is combined with an HPV test. If you have certain ...
Selective Gene Delivery to Head and Neck Cancer Cells via an
... RLU of luciferase/milligram of total cellular protein is shown graphically. The results are shown as the mean of multiple assays. The number of replicates (n) 5 3; error bars, SE. Ps were determined using the knob protein negative control (pp) and the knob protein negative control in HeLa cells (##) ...
... RLU of luciferase/milligram of total cellular protein is shown graphically. The results are shown as the mean of multiple assays. The number of replicates (n) 5 3; error bars, SE. Ps were determined using the knob protein negative control (pp) and the knob protein negative control in HeLa cells (##) ...
Negative Controls of Cell Proliferation: Human
... mented medium (Fig. 3). The inhibitory effect of CD human sera was not instantaneous; at the end of 3-4 days the popula tion increase was halted and remained stable for up to 30 days without media changes. That these cells were then still viable is suggested by increased cell proliferation rates whe ...
... mented medium (Fig. 3). The inhibitory effect of CD human sera was not instantaneous; at the end of 3-4 days the popula tion increase was halted and remained stable for up to 30 days without media changes. That these cells were then still viable is suggested by increased cell proliferation rates whe ...
Can We Use Nanotechnology to Treat Cancer?
... Although there have been many improvements in cancer treatments, cancer is still quite difficult to treat. One reason is because cancer medicine, called chemotherapy, can be quite difficult for a patient to receive. The medicines kids.frontiersin.org ...
... Although there have been many improvements in cancer treatments, cancer is still quite difficult to treat. One reason is because cancer medicine, called chemotherapy, can be quite difficult for a patient to receive. The medicines kids.frontiersin.org ...
Adducin-1 is essential for mitotic spindle assembly through its
... Figure 2. Phosphorylation of ADD1 at Ser12 and Ser355 by CDK1 is crucial for ADD1 association with mitotic spindles. (A) HeLa cells transiently expressing FLAG-ADD1 and mutants were stained for FLAG-ADD1, -tubulin, and DNA. (left) Representative confocal images from the cells in metaphase are show ...
... Figure 2. Phosphorylation of ADD1 at Ser12 and Ser355 by CDK1 is crucial for ADD1 association with mitotic spindles. (A) HeLa cells transiently expressing FLAG-ADD1 and mutants were stained for FLAG-ADD1, -tubulin, and DNA. (left) Representative confocal images from the cells in metaphase are show ...
Involvement of MMP-7 in invasion of pancreatic cancer cells through
... Francisco, California, USA), phosphorylated EGFR (p-EGFR), phosphorylated MEK (p-MEK), and phosphorylated ERK (pERK) (all Santa Cruz Biotechnology, Santa Cruz, California, USA) polyclonal antibodies in our study. These antibodies are crossreactive with hamster tissues.4–7 9 We also used fluorescein ...
... Francisco, California, USA), phosphorylated EGFR (p-EGFR), phosphorylated MEK (p-MEK), and phosphorylated ERK (pERK) (all Santa Cruz Biotechnology, Santa Cruz, California, USA) polyclonal antibodies in our study. These antibodies are crossreactive with hamster tissues.4–7 9 We also used fluorescein ...
as a PDF
... reporter genes were cotransfected into HeLa cells, together with constitutively expressed estrogen receptor (ER) constructs, demonstrate that activation of the transcription of the reporter genes by epidermal growth factor (EGF) and by cholera toxin with 3-isobutyl-1-methyl-xanthine, which elevate c ...
... reporter genes were cotransfected into HeLa cells, together with constitutively expressed estrogen receptor (ER) constructs, demonstrate that activation of the transcription of the reporter genes by epidermal growth factor (EGF) and by cholera toxin with 3-isobutyl-1-methyl-xanthine, which elevate c ...
Prostaglandin production by human trabecular cells: in vitro
... trabecular cells. To examine this possibility, media from well-defined trabecular cell material were assayed using specific radioimmunoassays. Morphologically differentiated human trabecular cells produced high levels of PGE 2 , and somewhat lower levels of PGF2o and 6KF la in the presence and absen ...
... trabecular cells. To examine this possibility, media from well-defined trabecular cell material were assayed using specific radioimmunoassays. Morphologically differentiated human trabecular cells produced high levels of PGE 2 , and somewhat lower levels of PGF2o and 6KF la in the presence and absen ...
A Mechanism Linking Extra Centrosomes to Chromosomal Instability
... Chromosome instability (CIN) is a hallmark of many tumors and correlates with the presence of extra centrosomes1-4. However, a direct mechanistic link between extra centrosomes and CIN has not been established. It has been proposed that extra centrosomes generate CIN by promoting multipolar anaphase ...
... Chromosome instability (CIN) is a hallmark of many tumors and correlates with the presence of extra centrosomes1-4. However, a direct mechanistic link between extra centrosomes and CIN has not been established. It has been proposed that extra centrosomes generate CIN by promoting multipolar anaphase ...
Fat Body Cells of the Frceze-tolerant
... In mammalian cells that are rapidly cooled, inoculative freezing of the intracellular fluid occurs between * 5 and -30'C depending on the cell type, however nucleation is typically blocked by the cell membrane at temperaturesabove - l5'C (Mazur, 1984).In comparison the fat body cells were frozen at ...
... In mammalian cells that are rapidly cooled, inoculative freezing of the intracellular fluid occurs between * 5 and -30'C depending on the cell type, however nucleation is typically blocked by the cell membrane at temperaturesabove - l5'C (Mazur, 1984).In comparison the fat body cells were frozen at ...
Anti-Cytochrome c Mouse mAb (7H8.2C12) Cat. No. AP1029
... space of mitochondria, loosely attached to the inner membrane and is an essential component of the mitochondrial respiratory chain. Early studies showed that during the course of an apoptotic response there was a rapid loss of function of cytochrome c in the dying cell. This was later shown to be du ...
... space of mitochondria, loosely attached to the inner membrane and is an essential component of the mitochondrial respiratory chain. Early studies showed that during the course of an apoptotic response there was a rapid loss of function of cytochrome c in the dying cell. This was later shown to be du ...
Optical Deformability as an Inherent Cell Marker for Testing
... Fixation was performed on two identically prepared slides using Acetone (99.9%) for 15 min. After permeablization for 10 min with TritonX-100, cells were additionally permeabilized and washed with Tween20 for 10 min. At this point, the negative-control slide received Phalloidin (P2141) at a concentr ...
... Fixation was performed on two identically prepared slides using Acetone (99.9%) for 15 min. After permeablization for 10 min with TritonX-100, cells were additionally permeabilized and washed with Tween20 for 10 min. At this point, the negative-control slide received Phalloidin (P2141) at a concentr ...
The_Cell_Cylce_and_Hallmarks_of_Cancer
... aging at the cellular level, had its genesis about 20 years ago when the dogma that maintained that cultured normal cells could replicate forever was overturned. Normal human and animal cells have a finite capacity to replicate and function whether they are cultured in vitro or transplanted as graft ...
... aging at the cellular level, had its genesis about 20 years ago when the dogma that maintained that cultured normal cells could replicate forever was overturned. Normal human and animal cells have a finite capacity to replicate and function whether they are cultured in vitro or transplanted as graft ...
New method for the analysis of cell cycle
... and monitoring of particular events of apoptosis are of importance not only in basic laboratory studies but also in the clinical setting. During the past decade, different methods for analysis of apoptosis have been developed (1,2,17). The methods using flow cytometry, by virtue of providing accurate ...
... and monitoring of particular events of apoptosis are of importance not only in basic laboratory studies but also in the clinical setting. During the past decade, different methods for analysis of apoptosis have been developed (1,2,17). The methods using flow cytometry, by virtue of providing accurate ...
Assessment of Aging in Saccharomyces Cerevisiae Yeast Mutants
... mutants that progressively reduced SOD2 expression and function [20]. In these mutant fruit flies life span was progressively shortened and age-dependent mortality was increased in conjunction with reduced SOD2 expression . The number of dead cells is one of the parameters that make it possible to d ...
... mutants that progressively reduced SOD2 expression and function [20]. In these mutant fruit flies life span was progressively shortened and age-dependent mortality was increased in conjunction with reduced SOD2 expression . The number of dead cells is one of the parameters that make it possible to d ...
Kerr et al 2016_04_08 - OPUS at UTS
... 2015; Scorrano et al., 2003). There are, however, discordant views on the contribution of PCDs in chlamydial infection biology. Whilst Vats and colleagues suggest that caspase-dependent PCD, or classical apoptosis, is prominent amongst Chlamydiainfected primary cervical epithelial cells (Vats et al. ...
... 2015; Scorrano et al., 2003). There are, however, discordant views on the contribution of PCDs in chlamydial infection biology. Whilst Vats and colleagues suggest that caspase-dependent PCD, or classical apoptosis, is prominent amongst Chlamydiainfected primary cervical epithelial cells (Vats et al. ...
Anti-Lunatic Fringe antibody
... autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscolios ...
... autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscolios ...
Photobleaching Substrates Characterized Using Fluorescence
... the merge images where the overlap is indicated by yellow. Fig. 1 shows that both Ku70- and Ku86-GFP efficiently associate with endogenous protein and are therefore faithful reporters of endogenous Ku. Ku70- and Ku86-GFP are highly mobile in cell nuclei To measure the mobility of Ku in cell nuclei, ...
... the merge images where the overlap is indicated by yellow. Fig. 1 shows that both Ku70- and Ku86-GFP efficiently associate with endogenous protein and are therefore faithful reporters of endogenous Ku. Ku70- and Ku86-GFP are highly mobile in cell nuclei To measure the mobility of Ku in cell nuclei, ...
The DNA helicase ChlR1 is required for sister chromatid cohesion in
... immunofluorescence signal was significantly reduced at all of the sites described above when ChlR1 expression was reduced with specific siRNAs (Fig. 2B and data not shown); control siRNAs did not affect ChlR1 localization. No specific staining was observed with pre-immune serum or when the 2075 anti ...
... immunofluorescence signal was significantly reduced at all of the sites described above when ChlR1 expression was reduced with specific siRNAs (Fig. 2B and data not shown); control siRNAs did not affect ChlR1 localization. No specific staining was observed with pre-immune serum or when the 2075 anti ...
Nuclear Localization of the Parafibromin Tumor Suppressor Protein
... subcellular localization of wild-type EGFP-HRPT2 and its deletion mutants illustrated in (A). Representative micrographs of the fluorescence analysis. Right, differential interference contrast is shown. D. Schematic diagram of parafibromin showing that NLS activity is restricted to the region betwee ...
... subcellular localization of wild-type EGFP-HRPT2 and its deletion mutants illustrated in (A). Representative micrographs of the fluorescence analysis. Right, differential interference contrast is shown. D. Schematic diagram of parafibromin showing that NLS activity is restricted to the region betwee ...
Macromomycin, an Inhibitor of the Membrane Function of Tumor Cells
... antitumor antibiotics have been isolated, there have been no reports on their interaction with cell membranes, although some of these substances are believed to exert their effects without penetrating the target cells. This study of the mode of action of MCR was initiated in the hope that it would f ...
... antitumor antibiotics have been isolated, there have been no reports on their interaction with cell membranes, although some of these substances are believed to exert their effects without penetrating the target cells. This study of the mode of action of MCR was initiated in the hope that it would f ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.