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... suggested that testicular cord cells may be the site of steroid production; synthesis would occur at a low rate and this fact together with differences in the sensitivity of the techniques used would explain the contradictory results. This conclusion would support Wolff's (1950) claim in the sense t ...
... suggested that testicular cord cells may be the site of steroid production; synthesis would occur at a low rate and this fact together with differences in the sensitivity of the techniques used would explain the contradictory results. This conclusion would support Wolff's (1950) claim in the sense t ...
New Insights of Transmembranal Mechanism and Subcellular
... MitoTracker and incubated with SWNT-PL-PEG-FITC for 30 min and observed under the microscope. Figure 1B shows that fluorescence emissions of SWNT-PL-PEG-FITC from either tumor cells or normal cells coincide with that of MitoTracker dye. However, in macrophages, lysosomes were the main source of fluo ...
... MitoTracker and incubated with SWNT-PL-PEG-FITC for 30 min and observed under the microscope. Figure 1B shows that fluorescence emissions of SWNT-PL-PEG-FITC from either tumor cells or normal cells coincide with that of MitoTracker dye. However, in macrophages, lysosomes were the main source of fluo ...
Neuroblastoma (NB) is the most common extra cranial solid tumor in
... minimal expression of DBH mRNA in the N cell line, contrary to what was shown with primer sets 1 and 3. DBH primer sets 1 and 2 produce overlapping products, and as a result should show very similar results. A possible explanation for this is that the N cell line expresses a transcript variant that ...
... minimal expression of DBH mRNA in the N cell line, contrary to what was shown with primer sets 1 and 3. DBH primer sets 1 and 2 produce overlapping products, and as a result should show very similar results. A possible explanation for this is that the N cell line expresses a transcript variant that ...
GCE AS/A Level 2400U10-1 – NEW AS BIOLOGY – Unit 1
... 2. Influenza, commonly known as “the flu”, is an infectious disease caused by the influenza virus, which infects the epithelial cells of the upper respiratory tract. The virus is composed of a protein capsid which surrounds the enzyme, RNA polymerase and its genetic material, RNA. ...
... 2. Influenza, commonly known as “the flu”, is an infectious disease caused by the influenza virus, which infects the epithelial cells of the upper respiratory tract. The virus is composed of a protein capsid which surrounds the enzyme, RNA polymerase and its genetic material, RNA. ...
Suppression of RAD21 gene expression decreases cell growth and
... MALDI-TOF mass spectrometry. Although the strongest inhibition of cellular proliferation was observed on day 6, total RNA for quantitation of gene expression was isolated from cells on day 2 post transfection. This earlier time point was used in order to detect the changes in RAD21 message that were ...
... MALDI-TOF mass spectrometry. Although the strongest inhibition of cellular proliferation was observed on day 6, total RNA for quantitation of gene expression was isolated from cells on day 2 post transfection. This earlier time point was used in order to detect the changes in RAD21 message that were ...
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... REEP4 and the REEP4/5 chimeric protein (REEP4 dMT). A total of 85% of wild-type REEP4-HA but only 13% of REEP4 dMT-HA copellet with microtubules. (C) HeLa cells expressing GFP-Sec61b and histone H2B-mcherry were imaged live at metaphase. Expression of RNAi-resistant wild-type REEP4 restores ER clear ...
... REEP4 and the REEP4/5 chimeric protein (REEP4 dMT). A total of 85% of wild-type REEP4-HA but only 13% of REEP4 dMT-HA copellet with microtubules. (C) HeLa cells expressing GFP-Sec61b and histone H2B-mcherry were imaged live at metaphase. Expression of RNAi-resistant wild-type REEP4 restores ER clear ...
Cell division - MrMcKennaBiologyPage
... • Nucleus of the cell controls c___ ell ivision including c____ d_______ ach of the t___ wo cells produced by cell • E___ division has a complete set of hromosomes and the s_____ ame c__________ information. oss enetic This ensures there is no l____ of g______ nformation in the t___ wo new b____cell ...
... • Nucleus of the cell controls c___ ell ivision including c____ d_______ ach of the t___ wo cells produced by cell • E___ division has a complete set of hromosomes and the s_____ ame c__________ information. oss enetic This ensures there is no l____ of g______ nformation in the t___ wo new b____cell ...
Technical Tip - Cytoskeleton, Inc.
... PFA-fixed cells as efficiently as phalloidin derivatives. SiR-tubulin labels microtubules only in ethyleneglycol-bis-succinimidyl-succinate (EGS)-fixed cells. However, a selective labeling of centrosomal microtubules of PFA-fixed cells was observed. SiR-actin and SiR-tubulin are not suitable for met ...
... PFA-fixed cells as efficiently as phalloidin derivatives. SiR-tubulin labels microtubules only in ethyleneglycol-bis-succinimidyl-succinate (EGS)-fixed cells. However, a selective labeling of centrosomal microtubules of PFA-fixed cells was observed. SiR-actin and SiR-tubulin are not suitable for met ...
BRYONIA ASPERA Research Article SHAMIM SAHRANAVARD
... Activity was attributed to the plant extracts expressing IC 50 < 100 μg/ml. The conventional anticancer drugs in clinical use, 5fluorouracil and tamoxifen were used as positive control. According to the data presented in table 1 none of the fractions showed important activity on cell lines HepG2 and ...
... Activity was attributed to the plant extracts expressing IC 50 < 100 μg/ml. The conventional anticancer drugs in clinical use, 5fluorouracil and tamoxifen were used as positive control. According to the data presented in table 1 none of the fractions showed important activity on cell lines HepG2 and ...
New strategies for cancer immunotherapy: targeting regulatory T cells
... Treg cells, whereas a complete portrait of tumor-resident Treg cells, including Treg-specific markers, is still lacking. In a recent edition of Immunity, two independent studies, led by Rudensky [4] and Pagani [5], shed light on the features of Treg cells infiltrating common human cancers. The Ruden ...
... Treg cells, whereas a complete portrait of tumor-resident Treg cells, including Treg-specific markers, is still lacking. In a recent edition of Immunity, two independent studies, led by Rudensky [4] and Pagani [5], shed light on the features of Treg cells infiltrating common human cancers. The Ruden ...
Cancer Metastases: So Close and So Far
... dominant, constitutive property of all cells (23). And the second, is that, from around 1907 to 1912, a group of American researchers lead by the physiologist Ross G. Harrison introduced tissue culture as an important methodological tool to study biological phenomena at large, including cancer (24). ...
... dominant, constitutive property of all cells (23). And the second, is that, from around 1907 to 1912, a group of American researchers lead by the physiologist Ross G. Harrison introduced tissue culture as an important methodological tool to study biological phenomena at large, including cancer (24). ...
Ras-Part 1
... But it’s a crazy idea 1. you need to find the right needle in a very large haystack 2.What if you need to find two or more needles?? ...
... But it’s a crazy idea 1. you need to find the right needle in a very large haystack 2.What if you need to find two or more needles?? ...
Diapositiva 1 - Medical Cannabis Bike Tour
... The combination of cannabinoids and other anticancer agents kills Glioma initiating cells in vitro. GICs are dissociated, counted and seeded at low cell density. After 5 days of incubation with cannabinoids and other compounds (P1), neurospheres are dissociated and total cells counted. Next, equal n ...
... The combination of cannabinoids and other anticancer agents kills Glioma initiating cells in vitro. GICs are dissociated, counted and seeded at low cell density. After 5 days of incubation with cannabinoids and other compounds (P1), neurospheres are dissociated and total cells counted. Next, equal n ...
Oncogenic Role of eIF-5A2 in the Development
... been increasing in Asian countries such as China and Singapore recently (1). Because of its insidious onset, 70% of ovarian cancer patients were diagnosed at advanced stage, and the prognosis is very poor with a 5-year survival rate of ⬍20% (2). Recurrent chromosomal changes in ovarian cancer have b ...
... been increasing in Asian countries such as China and Singapore recently (1). Because of its insidious onset, 70% of ovarian cancer patients were diagnosed at advanced stage, and the prognosis is very poor with a 5-year survival rate of ⬍20% (2). Recurrent chromosomal changes in ovarian cancer have b ...
Growth Inhibition of a Human Tumor Cell Strain
... prior to the addition of FUDR. The latter is based on analysis of approximately ~500 cells. ...
... prior to the addition of FUDR. The latter is based on analysis of approximately ~500 cells. ...
Ultrastructural, Cell Membrane, and Cytogenetic Characteristics of B
... The results clearly indicated a decrease in the cap-forming cleus and well-developed endoplasmic reticulum (Fig. 2). ability with F-Con A of the lymphocytes from BCL,-inoculated The proliferative response of BCL, cells to LPS was also mice beginning as early as 1 to 3 days after inoculation (Chart u ...
... The results clearly indicated a decrease in the cap-forming cleus and well-developed endoplasmic reticulum (Fig. 2). ability with F-Con A of the lymphocytes from BCL,-inoculated The proliferative response of BCL, cells to LPS was also mice beginning as early as 1 to 3 days after inoculation (Chart u ...
Distribution of Lysosomes in Cultured Cells and Ascites Tumor Cells
... (1952), Novikoff (1955) and Pearse (1960). fixed paraffin embedded tissues were unstatisfactory for use with the metal-salt method; he recommended that the technique was useful only for unfixed or formalinfixed frozen sections. Recently, lysosomes were demonstrated in cultured cells fixed with forma ...
... (1952), Novikoff (1955) and Pearse (1960). fixed paraffin embedded tissues were unstatisfactory for use with the metal-salt method; he recommended that the technique was useful only for unfixed or formalinfixed frozen sections. Recently, lysosomes were demonstrated in cultured cells fixed with forma ...
low-resistance junctions between cancer cells in various solid tumors
... Furshpan and Potter, on the other hand, have found that virally transformed cancer cell lines have apparently unimpaired coupling, whereas the degree of coupling in two cell lines (51801 and II), derived from a mouse sarcoma (S180) (10), depends on the time elapsed since renewal of medium (11 a ; un ...
... Furshpan and Potter, on the other hand, have found that virally transformed cancer cell lines have apparently unimpaired coupling, whereas the degree of coupling in two cell lines (51801 and II), derived from a mouse sarcoma (S180) (10), depends on the time elapsed since renewal of medium (11 a ; un ...
Cell mechanics regulation by cytoskeleton dynamics: Combined
... cytoskeleton . Moreover, we have shown remarkably different viscoelastic response of benign and malignant tumor cells at short timescales. However, the correlation between this mechanical signature and the structure of the cytoskeleton at high resolution is still unknown. In this specif ...
... cytoskeleton . Moreover, we have shown remarkably different viscoelastic response of benign and malignant tumor cells at short timescales. However, the correlation between this mechanical signature and the structure of the cytoskeleton at high resolution is still unknown. In this specif ...
Characterization of a murine model of metastatic human non
... testing has most commonly relied on subcutaneouslyimplanted human NSCLC xenografts grown in immunocompromised mice. Such models lack many of the features that are characteristic of the human disease, such as the development of metastases. In order to improve the success rate of anticancer drugs dest ...
... testing has most commonly relied on subcutaneouslyimplanted human NSCLC xenografts grown in immunocompromised mice. Such models lack many of the features that are characteristic of the human disease, such as the development of metastases. In order to improve the success rate of anticancer drugs dest ...
Inability of Methapyrilene to Induce Sister
... shown to induce SCE in different in vitro cell systems (inter alia Refs. 10 and 21), it seems likely that methapyrilene must be metabolically activated before SCE can be induced. We used early cultures of the diploid rat liver epithelial cells as the metabolizing cells, since they were shown to have ...
... shown to induce SCE in different in vitro cell systems (inter alia Refs. 10 and 21), it seems likely that methapyrilene must be metabolically activated before SCE can be induced. We used early cultures of the diploid rat liver epithelial cells as the metabolizing cells, since they were shown to have ...
File - Wk 1-2
... lymphatic spread however, the cancer must break through the surrounding tissue and infiltrate a pathway for spreading. To break through surrounding tissue, the cells must degrade and invade the extracellular matrix. In the body, the ECM is constantly broken down and restructured by a counteractive ...
... lymphatic spread however, the cancer must break through the surrounding tissue and infiltrate a pathway for spreading. To break through surrounding tissue, the cells must degrade and invade the extracellular matrix. In the body, the ECM is constantly broken down and restructured by a counteractive ...
oncogene
... But it’s a crazy idea 1. you need to find the right needle in a very large haystack 2.What if you need to find two or more needles?? ...
... But it’s a crazy idea 1. you need to find the right needle in a very large haystack 2.What if you need to find two or more needles?? ...
Formation of Wound Tissue of Gracilaria chorda Holmes
... agar products of Gracilaria spp. are in great demand, this genus is cultured all over the world. There are various methods for cultivation, including transplantation, attachment of spores or thalli to ropes, and regeneration of fragments on the sea bottom. The regeneration of plants has been an espe ...
... agar products of Gracilaria spp. are in great demand, this genus is cultured all over the world. There are various methods for cultivation, including transplantation, attachment of spores or thalli to ropes, and regeneration of fragments on the sea bottom. The regeneration of plants has been an espe ...
Cytogenetic analysis
... of trisomy rescue. If a trisomic conception undergoes trisomic rescue in certain cells, including those that are destined to become the fetus, then the remaining trisomy cells may be confined to the placenta ...
... of trisomy rescue. If a trisomic conception undergoes trisomic rescue in certain cells, including those that are destined to become the fetus, then the remaining trisomy cells may be confined to the placenta ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.