Full Text - Verlag der Zeitschrift für Naturforschung
... gathering was significantly inhibited. In most of the model experiments described below, we employed a polypropylene tube with 2 mm in diameter but finer tubes (diameters of 1.0 mm and 1.2 mm) were also shown to be applicable. All tubes showed successful cell gathering and cell migration of green pa ...
... gathering was significantly inhibited. In most of the model experiments described below, we employed a polypropylene tube with 2 mm in diameter but finer tubes (diameters of 1.0 mm and 1.2 mm) were also shown to be applicable. All tubes showed successful cell gathering and cell migration of green pa ...
HAUSP, a deubiquitinating enzyme for p53, is polyubiquitinated
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
... regions between nucleosomes. As expected, rHAUSP-transfected HeLa cells died completely. We observed extracted genomic DNA from apoptotic HeLa cells induced by rHAUSP and rHAUSP (C224S), visualized as DNA ladders, indicating that these cells are apoptotic (data not shown). We also investigated the e ...
RNA nuclear export is blocked by poliovirus 2A protease and is
... involve many components of the NPC. Nup98, Nup153 and Nup62 have been reported to be cleaved in PV-infected cells (Gustin and Sarnow, 2001; Gustin and Sarnow, 2002; Park et al., 2008). Nup98 is proteolyzed soon after PV infection, whereas Nup153 and Nup62 cleavage need viral replication and, consequ ...
... involve many components of the NPC. Nup98, Nup153 and Nup62 have been reported to be cleaved in PV-infected cells (Gustin and Sarnow, 2001; Gustin and Sarnow, 2002; Park et al., 2008). Nup98 is proteolyzed soon after PV infection, whereas Nup153 and Nup62 cleavage need viral replication and, consequ ...
staxanthin, a kotenoid without Vitamin A Activity,
... Experiment 2. Carotenoids action on Ab production was tested with the use of primed B cells and the same experimental system as described in Experiment 1 (Fig. 11.Nameiy, Thl (A.E7 cells) and Th2 (CDC3.5 cells] cell clones were stimulated with the same doses of Ag as in Experiment 1. The culture was ...
... Experiment 2. Carotenoids action on Ab production was tested with the use of primed B cells and the same experimental system as described in Experiment 1 (Fig. 11.Nameiy, Thl (A.E7 cells) and Th2 (CDC3.5 cells] cell clones were stimulated with the same doses of Ag as in Experiment 1. The culture was ...
Supporting Online Material for
... direct the synthesis of shRNAs targeting LacZ or Fbxl3, as indicated. In this experiment the shRNA construct 3 to mouse Fbxl3 (see fig. S4A) was used. Cells were asynchronously grown to confluence in a medium containing 10% fetal bovine serum (Asynch.). Asynchronous cells were serum starved for 16 h ...
... direct the synthesis of shRNAs targeting LacZ or Fbxl3, as indicated. In this experiment the shRNA construct 3 to mouse Fbxl3 (see fig. S4A) was used. Cells were asynchronously grown to confluence in a medium containing 10% fetal bovine serum (Asynch.). Asynchronous cells were serum starved for 16 h ...
Isolation and characterization of a marine magnetotactic spirillum
... Laboratoire International Associé de la Bio-Minéralization et Nano-Structures, CNRS-Marseille, 13009 Marseille, France Received 4 December 2009; accepted 3 February 2010 ...
... Laboratoire International Associé de la Bio-Minéralization et Nano-Structures, CNRS-Marseille, 13009 Marseille, France Received 4 December 2009; accepted 3 February 2010 ...
Cytotoxicity of resin monomers on human gingival - GCP
... TC50 values have been frequently used in cytotoxicity studies and a wide range of TC50 values have been reported for resin monomers [7,18–20]. The results of statistical analysis in this study showed that there was a significant difference in TC50 values between three HGF cultures and between HaCaT c ...
... TC50 values have been frequently used in cytotoxicity studies and a wide range of TC50 values have been reported for resin monomers [7,18–20]. The results of statistical analysis in this study showed that there was a significant difference in TC50 values between three HGF cultures and between HaCaT c ...
Glonal analysis of the crustacean segment
... (3)) have been formed by cells of the blastodermic germ disc. Of these, rows (2) and (3) have already been divided once by a mitotic wave to form two rows each. On the animal's right side, the two cells nearest to the median line cleave for the second time and two cells of row (1) cleave for the fir ...
... (3)) have been formed by cells of the blastodermic germ disc. Of these, rows (2) and (3) have already been divided once by a mitotic wave to form two rows each. On the animal's right side, the two cells nearest to the median line cleave for the second time and two cells of row (1) cleave for the fir ...
rumex l. species induce apoptosis in 1301, eol-1 and h
... improving the quality of life of patients, and potentially in increasing their survival (3). Particular popularity of EssiacÆ as complementary therapy among breast cancer survivors and patients may be related to the first described use of EssiacÆ tonic as a cure for a breast lump (2). It has been al ...
... improving the quality of life of patients, and potentially in increasing their survival (3). Particular popularity of EssiacÆ as complementary therapy among breast cancer survivors and patients may be related to the first described use of EssiacÆ tonic as a cure for a breast lump (2). It has been al ...
A novel microtubule-modulating noscapinoid
... Figure 1 ‘Kinder and gentler’ effects of EM011 on microtubules. Dynamic instability behavior of fluorescently labeled interphase microtubules in the thin lamellar edge of MCF-7 cells expressing GFP-tubulin was imaged by time-lapse microscopy.(ai) Time-lapse sequence of video frames, 3 s apart, showi ...
... Figure 1 ‘Kinder and gentler’ effects of EM011 on microtubules. Dynamic instability behavior of fluorescently labeled interphase microtubules in the thin lamellar edge of MCF-7 cells expressing GFP-tubulin was imaged by time-lapse microscopy.(ai) Time-lapse sequence of video frames, 3 s apart, showi ...
Dishevelled 2 signaling promotes self
... new insights into the disease.Several groups have identified a sub-population of stemlike, cancer cells in brain tumors, using biomarker analysis and culturing techniques similar to those used to characterize normal neural stem cells. These cells demonstrate a significant tumor-initiating ability, ...
... new insights into the disease.Several groups have identified a sub-population of stemlike, cancer cells in brain tumors, using biomarker analysis and culturing techniques similar to those used to characterize normal neural stem cells. These cells demonstrate a significant tumor-initiating ability, ...
The Differentiation of Contact Cells and Isolation
... found that there was little difference between the protective layer and the isotropic layer in terms of their ultrastructure and chemical compositions prior to lignification. Thus, they concluded that these two layers might have the same origin and that lignification might begin at different times i ...
... found that there was little difference between the protective layer and the isotropic layer in terms of their ultrastructure and chemical compositions prior to lignification. Thus, they concluded that these two layers might have the same origin and that lignification might begin at different times i ...
Locomotion of Fundulus Deep Cells during Gastrulation1
... Good information on the distribution of adhesions of a rounded cell to the surfaces of other cells comes from other deep cells that appeared not to be moving, but whose locomotor activity was not actually known with certainty at the time of fixation, and from certain rounded cells adhering to other ...
... Good information on the distribution of adhesions of a rounded cell to the surfaces of other cells comes from other deep cells that appeared not to be moving, but whose locomotor activity was not actually known with certainty at the time of fixation, and from certain rounded cells adhering to other ...
Simulating the Hallmarks of Cancer
... dividing. In culture, human cells are limited to 50– 70 divisions (the Hayflick limit [11]), although most die before exhausting this potential. This limitation prevents many tumors from growing large enough to be seen without a microscope. Experiments show, however, that cells can acquire limitless ...
... dividing. In culture, human cells are limited to 50– 70 divisions (the Hayflick limit [11]), although most die before exhausting this potential. This limitation prevents many tumors from growing large enough to be seen without a microscope. Experiments show, however, that cells can acquire limitless ...
Named cells in dermatology - Indian Journal of Dermatology
... 3. Eczematous disorders: Tadpole cells 4. Metabolic and storage disorders: Gargoyle cells, Gaucher’s cell, Xanthoma cells 5. Tumors: Basalioma cells, Basophilic and shadow cells, Doughnut cells, Flower cells, Granular cells, Halo cells, Hibernoma cells, Paget cells, Sezary cells, Signet ring cell ...
... 3. Eczematous disorders: Tadpole cells 4. Metabolic and storage disorders: Gargoyle cells, Gaucher’s cell, Xanthoma cells 5. Tumors: Basalioma cells, Basophilic and shadow cells, Doughnut cells, Flower cells, Granular cells, Halo cells, Hibernoma cells, Paget cells, Sezary cells, Signet ring cell ...
Seminars in Cancer Biology Invasion emerges from cancer cell
... the population level. Discovery of other tumor suppressor genes was promoted by multiscale links from population to molecular genetics studies [5], but again the intermediate cell scales remain largely unexplored. Conversely, in non-familiar sporadic cancers, fundamental insights have been obtained ...
... the population level. Discovery of other tumor suppressor genes was promoted by multiscale links from population to molecular genetics studies [5], but again the intermediate cell scales remain largely unexplored. Conversely, in non-familiar sporadic cancers, fundamental insights have been obtained ...
Visualizing_the_mechanical_activation_of_Src
... Phosphorylation of Tyr still occurs if just one Tyr is mutated but binding doesn’t Neighboring amino acids are important for SH2 binding ...
... Phosphorylation of Tyr still occurs if just one Tyr is mutated but binding doesn’t Neighboring amino acids are important for SH2 binding ...
STK31 maintains the undifferentiated state of colon cancer cells
... samples showing varied signal intensities in at least 5% of the cancer cells in the samples. The expression of STK31 appeared to be less heterogeneous in moderately to poorly differentiated cancers (31% heterogeneous expression) when compared with well-differentiated cancers (50% heterogeneous expre ...
... samples showing varied signal intensities in at least 5% of the cancer cells in the samples. The expression of STK31 appeared to be less heterogeneous in moderately to poorly differentiated cancers (31% heterogeneous expression) when compared with well-differentiated cancers (50% heterogeneous expre ...
Probing the invasiveness of prostate cancer cells in a 3D
... different steps during the metastasis?” To answer this, it is crucial to set up an in vitro microscale experiment where cancer cells can be released to invade vacant areas and where their spatial motility and proliferation can be analyzed to compare the invasion potentials quantitatively. Our experi ...
... different steps during the metastasis?” To answer this, it is crucial to set up an in vitro microscale experiment where cancer cells can be released to invade vacant areas and where their spatial motility and proliferation can be analyzed to compare the invasion potentials quantitatively. Our experi ...
2.3 Cell Continuity
... Q. Name a gas that would be needed for the growth of tissue in the laboratory. Animal cell ...
... Q. Name a gas that would be needed for the growth of tissue in the laboratory. Animal cell ...
Approaches for Monitoring Nuclear Translation
... the cellular pools of unlabeled amino acids are relatively high, little radiolabel is incorporated during short pulses, necessitating lengthy autoradiographic exposures. This problem can be mitigated by the use of isolated nuclei or permeabilized cells, where cellular pools are washed away (Subheadi ...
... the cellular pools of unlabeled amino acids are relatively high, little radiolabel is incorporated during short pulses, necessitating lengthy autoradiographic exposures. This problem can be mitigated by the use of isolated nuclei or permeabilized cells, where cellular pools are washed away (Subheadi ...
MATH 100 V1A
... 500 = P (0) = Ae0 = A. Therefore our equation simplifies to P (t) = 500ekt . Since there are 900 cells after 24 hours, we must have that 900 = P (24) = e24k . Solving this log( 9 ) for k gives us that k = 245 . So the equation that describes the number of cells in the culture as a function of time i ...
... 500 = P (0) = Ae0 = A. Therefore our equation simplifies to P (t) = 500ekt . Since there are 900 cells after 24 hours, we must have that 900 = P (24) = e24k . Solving this log( 9 ) for k gives us that k = 245 . So the equation that describes the number of cells in the culture as a function of time i ...
Role of Cytoskeleton in Controlling the Disorder Strength of Cellular
... Culture Collection, Manassas, VA) for 5 min each. Cells were then permeabilized using 0.1% Triton X-100 in PBS for 20 min at room temperature. Blocking was carried out using 1% bovine serum albumin in PBS for 20 min. Cells were then either incubated for 2 h with AlexaFluor 555 conjugated to anti-b-t ...
... Culture Collection, Manassas, VA) for 5 min each. Cells were then permeabilized using 0.1% Triton X-100 in PBS for 20 min at room temperature. Blocking was carried out using 1% bovine serum albumin in PBS for 20 min. Cells were then either incubated for 2 h with AlexaFluor 555 conjugated to anti-b-t ...
Modulation of the Cell Cycle-dependent
... progression from G, to S phase (Fig. 2). Fig. 2, A and C, shows that under normal conditions the number of G, cells in a Gì phase-enriched cell population declined progressively with time as individual cells transited the cell cycle. At 16 h, the number of G, cells reached a minimum (31%), while 44 ...
... progression from G, to S phase (Fig. 2). Fig. 2, A and C, shows that under normal conditions the number of G, cells in a Gì phase-enriched cell population declined progressively with time as individual cells transited the cell cycle. At 16 h, the number of G, cells reached a minimum (31%), while 44 ...
Alfy, a novel FYVE-domain-containing protein associated with
... to study the functions of various proteins that contain FYVE or PX domains, and several mammalian PtdIns(3)P effectors in endocytic membrane trafficking have already been identified (Simonsen et al., 1998; Raiborg et al., 2002; Nielsen et al., 2000; Ikonomov et al., 2003). Although three PtdIns(3)P ...
... to study the functions of various proteins that contain FYVE or PX domains, and several mammalian PtdIns(3)P effectors in endocytic membrane trafficking have already been identified (Simonsen et al., 1998; Raiborg et al., 2002; Nielsen et al., 2000; Ikonomov et al., 2003). Although three PtdIns(3)P ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.