DeadEnd Fluorometric TUNEL System Technical
... Cells dying by apoptosis often fragment into membrane-bound apoptotic bodies that are readily phagocytosed and digested by macrophages or by neighboring cells without generating an inflammatory response. This is in contrast to the type of cell death known as necrosis, characterized by cell swelling, ...
... Cells dying by apoptosis often fragment into membrane-bound apoptotic bodies that are readily phagocytosed and digested by macrophages or by neighboring cells without generating an inflammatory response. This is in contrast to the type of cell death known as necrosis, characterized by cell swelling, ...
Anticancer Properties of Cardiac Glycosides
... because cyclin D1 is a subunit of cyclin dependent kinase (Cdk)-4 and Cdk-6, which are responsible for cell cycle progression from G1 to S phase [37], these changes prevent cells from entering the next phase of the cycle. Likewise, digitoxin causes cell cycle arrest in G2/M in a dose-dependent manne ...
... because cyclin D1 is a subunit of cyclin dependent kinase (Cdk)-4 and Cdk-6, which are responsible for cell cycle progression from G1 to S phase [37], these changes prevent cells from entering the next phase of the cycle. Likewise, digitoxin causes cell cycle arrest in G2/M in a dose-dependent manne ...
EMT in developmental morphogenesis
... Carcinomas, cancers of epithelial origin, constitute the majority of all cancers. Loss of epithelial characteristics is an early step in carcinoma progression. Malignant transformation and metastasis involve additional loss of cell-cycle control and gain of migratory behaviors. Understanding the rel ...
... Carcinomas, cancers of epithelial origin, constitute the majority of all cancers. Loss of epithelial characteristics is an early step in carcinoma progression. Malignant transformation and metastasis involve additional loss of cell-cycle control and gain of migratory behaviors. Understanding the rel ...
cell-substrate contacts in cultured chick embryonic cells
... white rim seen a few minutes after plating in all tissues studied (Fig. 3). These point contacts were mobile and were directly correlated with blebs visible by PC on the lower surface of the cells. After 30 min larger broad grey contacts were common beneath cells that were still rounded in PC (Fig. ...
... white rim seen a few minutes after plating in all tissues studied (Fig. 3). These point contacts were mobile and were directly correlated with blebs visible by PC on the lower surface of the cells. After 30 min larger broad grey contacts were common beneath cells that were still rounded in PC (Fig. ...
The Golgi Stack Reassembles during Telophase before Arrival of
... hE Golgi apparatus in animal cells fragments and vesiculates at the onset of mitosis (Warren, 1993). The disassembly pathway involves the generation of several hundred Golgi clusters as a result of vesiculation (Lucocq et al., 1987) which subsequently shed thousands of vesicles into the mitotic cell ...
... hE Golgi apparatus in animal cells fragments and vesiculates at the onset of mitosis (Warren, 1993). The disassembly pathway involves the generation of several hundred Golgi clusters as a result of vesiculation (Lucocq et al., 1987) which subsequently shed thousands of vesicles into the mitotic cell ...
Potential effect of spliceosome inhibition in small cell lung
... therapeutic vulnerability in MYC-driven breast cancers, we evaluated the efficacy of a spliceosome inhibitor in SCLC cell lines and analyzed the correlation with MYC status. Among 23 SCLC cell lines examined, eight showed high MYC protein expression (> 80% positive cells) by immunohistochemistry (IH ...
... therapeutic vulnerability in MYC-driven breast cancers, we evaluated the efficacy of a spliceosome inhibitor in SCLC cell lines and analyzed the correlation with MYC status. Among 23 SCLC cell lines examined, eight showed high MYC protein expression (> 80% positive cells) by immunohistochemistry (IH ...
JOHN MASON PAWELEK, Ph
... and poor survival in breast carcinoma. Breast Cancer Research Conference, Yale Cancer Center. 01/13/04 “Origins of cancer.” Spotlight on Research: Yale Animal Care and Use Seminar Series. 02/12/04. “Cancer.” Nursing Program, University of South Dakota, Sioux Falls, S.D. 02/13/04. “BMT-tumor hybrids ...
... and poor survival in breast carcinoma. Breast Cancer Research Conference, Yale Cancer Center. 01/13/04 “Origins of cancer.” Spotlight on Research: Yale Animal Care and Use Seminar Series. 02/12/04. “Cancer.” Nursing Program, University of South Dakota, Sioux Falls, S.D. 02/13/04. “BMT-tumor hybrids ...
THE MAMMALIAN CELL VIRUS RELATIONSHIP (From the
... at 4°C. (used within a week of opening); and (c) ethylenediamine sodium tetraazetate (versene) for addition to the primary virus suspension in final concentration of 5 X 10-a M to prevent inactivation of nucleic acid by metal ions (11). Three to six ml. quantities of virus were mixed with equal amou ...
... at 4°C. (used within a week of opening); and (c) ethylenediamine sodium tetraazetate (versene) for addition to the primary virus suspension in final concentration of 5 X 10-a M to prevent inactivation of nucleic acid by metal ions (11). Three to six ml. quantities of virus were mixed with equal amou ...
SYSTEM ANALYSIS MORPHOGENESIS BREAST CANCER
... cells Excludes qualitative uniqueness of these states at the "cell." Second, in systems such as EPE properties of any cell are determined by all the other cells. Hence, for any of many differentiated cells need some preliminary development of type EPE increase integrity and which was evident in the ...
... cells Excludes qualitative uniqueness of these states at the "cell." Second, in systems such as EPE properties of any cell are determined by all the other cells. Hence, for any of many differentiated cells need some preliminary development of type EPE increase integrity and which was evident in the ...
Tasquinimod Modulates Suppressive Myeloid Cells and Enhances
... trial to test the effect of tasquinimod in the same patient population is ongoing (NCT01234311). Tasquinimod has been shown to inhibit prostate cancer growth and metastasis in animal models (16–18). Results from these studies have suggested that the antiangiogenic property of this molecule may be re ...
... trial to test the effect of tasquinimod in the same patient population is ongoing (NCT01234311). Tasquinimod has been shown to inhibit prostate cancer growth and metastasis in animal models (16–18). Results from these studies have suggested that the antiangiogenic property of this molecule may be re ...
Nuclear-cytoplasmic distribution of Inh2
... subunit of PP1 (Huang et al., 1999; Yang et al., 2000). It is unclear if these different sites target Inh2 to different PP1 holoenzymes in order to respond to changes in cell signaling. Inh2 is a 23 kDa phosphoprotein, small enough to passively diffuse through nuclear pores, yet it contains both a p ...
... subunit of PP1 (Huang et al., 1999; Yang et al., 2000). It is unclear if these different sites target Inh2 to different PP1 holoenzymes in order to respond to changes in cell signaling. Inh2 is a 23 kDa phosphoprotein, small enough to passively diffuse through nuclear pores, yet it contains both a p ...
Differential induction of cytotoxicity and apoptosis by influenza virus
... 1 : 14 (SE 5±1) for A}Fiji. + Production of UV-inactivated virus. Allantoic fluid harvested from 50 infected eggs was clarified from cell debris by low-speed centrifugation (1000 g, 30 min at 4 °C) and then spun at 80 000 g for 90 min at 4 °C to pellet the virus. The pellets were soaked in TSE buffe ...
... 1 : 14 (SE 5±1) for A}Fiji. + Production of UV-inactivated virus. Allantoic fluid harvested from 50 infected eggs was clarified from cell debris by low-speed centrifugation (1000 g, 30 min at 4 °C) and then spun at 80 000 g for 90 min at 4 °C to pellet the virus. The pellets were soaked in TSE buffe ...
RNA-dependent disassembly of nuclear bodies
... Fusion with iPNBs is not a major mechanism of nucleolus reformation EGFP-B23 transfer from the nucleoplasm to nucleoli might be a consequence of directed motion and fusion of iPNBs with nucleoli. To analyze iPNB motion, a time-lapse series of HeLa cells expressing EGFP-B23 was obtained using a confo ...
... Fusion with iPNBs is not a major mechanism of nucleolus reformation EGFP-B23 transfer from the nucleoplasm to nucleoli might be a consequence of directed motion and fusion of iPNBs with nucleoli. To analyze iPNB motion, a time-lapse series of HeLa cells expressing EGFP-B23 was obtained using a confo ...
Lenti-X™ Tet-On® Advanced Expression System User
... • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in part to the prokaryotic nature of these components acting within the conte ...
... • Highly specific. The binding of the Tet-Advanced TetR-derived domains to the tetO target sequences of PTight is highly specific and does not activate off-target cellular genes. This high degree of specificity may be due in part to the prokaryotic nature of these components acting within the conte ...
Roles of ARFRP1 (ADP-ribosylation factor
... Department of Cell Biology and Neuroscience, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan ...
... Department of Cell Biology and Neuroscience, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan ...
The nuclear membrane in multidrug resistance
... Multidrug resistance (MDR) in bladder cancer is common, with up to 75% of patients expressing markers of MDR at presentation [1,2]. Anthracyclines and mitomycin C belong to the MDR family of drugs and have been widely used as adjuvant intravesical chemotherapy for superficial bladder cancer [3–7]. A ...
... Multidrug resistance (MDR) in bladder cancer is common, with up to 75% of patients expressing markers of MDR at presentation [1,2]. Anthracyclines and mitomycin C belong to the MDR family of drugs and have been widely used as adjuvant intravesical chemotherapy for superficial bladder cancer [3–7]. A ...
Slides - Workforce Development in Stem Cell Research
... • Explant of chicken heart placed on glass slide, fed with clotted serum and chicken embryo extract • Claimed it continuously cultured for 34 years (long after the lifespan of a chicken) with no changes in appearance or growth rate • Hypothesized that all cells were immortal in that they could grow ...
... • Explant of chicken heart placed on glass slide, fed with clotted serum and chicken embryo extract • Claimed it continuously cultured for 34 years (long after the lifespan of a chicken) with no changes in appearance or growth rate • Hypothesized that all cells were immortal in that they could grow ...
Intra-epithelial mast cells in human airway epithelium
... A morphological and quantitative study was performed on the respiratory epithelium of human distal airways in 20 lungs removed at operation for the treatment of carcinoma. Using 1 ,um araldite sections stained with Toluidine Blue for light microscopy, with further electron microscopic study where re ...
... A morphological and quantitative study was performed on the respiratory epithelium of human distal airways in 20 lungs removed at operation for the treatment of carcinoma. Using 1 ,um araldite sections stained with Toluidine Blue for light microscopy, with further electron microscopic study where re ...
Cell Proliferation Kinetics and Drug Sensitivity of
... The time course of survival of cells exposed to the 5phase-specific agents, ara-C, HU, and high-specific-activity [3H)TdR is shown in Chart 3. The results show that the percentage of cells killed by these agents correlates well with the percentage of S-phase cells in the population (see Table 2). Th ...
... The time course of survival of cells exposed to the 5phase-specific agents, ara-C, HU, and high-specific-activity [3H)TdR is shown in Chart 3. The results show that the percentage of cells killed by these agents correlates well with the percentage of S-phase cells in the population (see Table 2). Th ...
Antifolate Polyglutamylation and Competitive Drug Displacement at
... (0.2 nmol) were formed from radiolabeled cofactor, consistent with sustained dihydrofolate reducÃ-aseactivity. These findings support a role for the activation of dihydrofolate reducÃ-ase as an important component of the reversal of the effects of diaminoantifolates by leucovorin, presumably by a co ...
... (0.2 nmol) were formed from radiolabeled cofactor, consistent with sustained dihydrofolate reducÃ-aseactivity. These findings support a role for the activation of dihydrofolate reducÃ-ase as an important component of the reversal of the effects of diaminoantifolates by leucovorin, presumably by a co ...
Poster GIGA DAY Lechanteur
... 2Laboratory of Experimental Pathology, GIGA-CANCER University of Liege, Liège, Belgium ...
... 2Laboratory of Experimental Pathology, GIGA-CANCER University of Liege, Liège, Belgium ...
Anthraquinone emodin inhibits human cancer
... channel P2X7 receptor (P2X7R) is strongly upregulated in many tumors and cancer cells, and has an important role in cancer cell invasion associated with metastases. Emodin (1,3,8-trihydroxy6-methylanthraquinone) is an anthraquinone derivative originally isolated from Rheum officinale Baill known for ...
... channel P2X7 receptor (P2X7R) is strongly upregulated in many tumors and cancer cells, and has an important role in cancer cell invasion associated with metastases. Emodin (1,3,8-trihydroxy6-methylanthraquinone) is an anthraquinone derivative originally isolated from Rheum officinale Baill known for ...
Homeostatic maintenance of pathogen
... (Salmonella 79.7% ⫾ 3.2%; GAS 85% ⫾ 3.6%), consistent with the possibility that AQP1 functions at the PCV membrane. We further investigated if AQP1 colocalized with PCV in wild-type cells. As AQP1 levels could not reliably be detected by immunofluorescence in tbk1+/+ cells (Fig. S1), we utilized a m ...
... (Salmonella 79.7% ⫾ 3.2%; GAS 85% ⫾ 3.6%), consistent with the possibility that AQP1 functions at the PCV membrane. We further investigated if AQP1 colocalized with PCV in wild-type cells. As AQP1 levels could not reliably be detected by immunofluorescence in tbk1+/+ cells (Fig. S1), we utilized a m ...
Human embryonic stem cells express a unique set
... The first human ES (hES) cell line was established only recently (Thomson et al., 1998), and 12 lines are publicly available worldwide (NIH Human Embryonic Stem Cell Registry). Despite their great potential, hES cells have not been a prolific source of information. This is mainly due to the technica ...
... The first human ES (hES) cell line was established only recently (Thomson et al., 1998), and 12 lines are publicly available worldwide (NIH Human Embryonic Stem Cell Registry). Despite their great potential, hES cells have not been a prolific source of information. This is mainly due to the technica ...
Involvement of the Mismatch Repair System in Temozolomide
... obtain comparable levels of cell growth impairment in TK6 cells (data not shown). As a control, similar experiments were performed with the unrelated antitumor drug etoposide, which is known to exert its cytotoxic effects through the inhibition of topoisomerase II (Bender et al., 1990). In this case ...
... obtain comparable levels of cell growth impairment in TK6 cells (data not shown). As a control, similar experiments were performed with the unrelated antitumor drug etoposide, which is known to exert its cytotoxic effects through the inhibition of topoisomerase II (Bender et al., 1990). In this case ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.