cathepsin-d is required for the growth of fibroblasts in - HAL
... D231N mutated cath-D devoid of proteolytic activity (D231N). Only cancer cells secreting wild-type or D231N pro-cath-D stimulated CD55-/-SV40 fibroblast outgrowth, whereas control cells had no effect (Figure 1B) suggesting that secreted pro-cath-D might promote 3D outgrowth of fibroblasts in a para ...
... D231N mutated cath-D devoid of proteolytic activity (D231N). Only cancer cells secreting wild-type or D231N pro-cath-D stimulated CD55-/-SV40 fibroblast outgrowth, whereas control cells had no effect (Figure 1B) suggesting that secreted pro-cath-D might promote 3D outgrowth of fibroblasts in a para ...
High frequency precise multiple-base reversion of
... chloroform extraction, again diluted 1:5, and seeded on HeLa cells to produce three independent primary clonal pools designated C1, C2, and C3 (Table 1, experiment A). Plaque assays ofprimary pools C1, C2, and C3 at 330C and 390C again demonstrated non-ts revertant frequencies 2-3 x 10-5 (Table 1, e ...
... chloroform extraction, again diluted 1:5, and seeded on HeLa cells to produce three independent primary clonal pools designated C1, C2, and C3 (Table 1, experiment A). Plaque assays ofprimary pools C1, C2, and C3 at 330C and 390C again demonstrated non-ts revertant frequencies 2-3 x 10-5 (Table 1, e ...
Amino Acid Analogs, and Nucleases on the Synthesis of DNA in
... involve protein synthesis (35). A recent report by Kellenberger et al. (16) indicates that synthesis of both RNA and DNA in E. coli operates by a mechanism that requires amino acids. However, “relaxedstrain― mutants of E. coli were found in which DNA synthesis would proceed in the ab sence of a ...
... involve protein synthesis (35). A recent report by Kellenberger et al. (16) indicates that synthesis of both RNA and DNA in E. coli operates by a mechanism that requires amino acids. However, “relaxedstrain― mutants of E. coli were found in which DNA synthesis would proceed in the ab sence of a ...
Pomegranate Ellagitannin–Derived Compounds Exhibit
... Estrogen stimulates the proliferation of breast cancer cells and the growth of estrogen-responsive tumors. The aromatase enzyme, which converts androgen to estrogen, plays a key role in breast carcinogenesis. The pomegranate fruit, a rich source of ellagitannins (ET), has attracted recent attention ...
... Estrogen stimulates the proliferation of breast cancer cells and the growth of estrogen-responsive tumors. The aromatase enzyme, which converts androgen to estrogen, plays a key role in breast carcinogenesis. The pomegranate fruit, a rich source of ellagitannins (ET), has attracted recent attention ...
Histone H3 phosphorylation is required for the initiation, but not
... phosphorylation only after 5 hours of treatment with 0.5 µM OA (data not shown). Similarly, mitotic chromosome morphology was observed in less than 1% of the cells. In the absence of OA treatment, H3 phosphorylation was undetectable during early interphase in HeLa cells (Fig. 3G,H). Untreated CHO an ...
... phosphorylation only after 5 hours of treatment with 0.5 µM OA (data not shown). Similarly, mitotic chromosome morphology was observed in less than 1% of the cells. In the absence of OA treatment, H3 phosphorylation was undetectable during early interphase in HeLa cells (Fig. 3G,H). Untreated CHO an ...
The enhancement of histone H4 and H2A serine 1 phosphorylation
... recognized the phospho-H4S1 peptide, but not the unphosphorylated peptide (Fig. 1B). In addition, preincubation of the antibody with excess phosphorylated peptide, but not with the unmodified peptide, competed away the binding of this antibody to the ELISA plate bound peptides, further confirming th ...
... recognized the phospho-H4S1 peptide, but not the unphosphorylated peptide (Fig. 1B). In addition, preincubation of the antibody with excess phosphorylated peptide, but not with the unmodified peptide, competed away the binding of this antibody to the ELISA plate bound peptides, further confirming th ...
Folliculin directs the formation of a Rab34–RILP
... unbiased assay to assess these effects across a population of cells. To this end, images of cells were segmented by scaling the perimeter in 10% decrements. Cumulative integrated LAMP1 intensity (relative to the whole cell) was then plotted (Figs 1E–G and EV1A). Curve fitting using nonlinear regress ...
... unbiased assay to assess these effects across a population of cells. To this end, images of cells were segmented by scaling the perimeter in 10% decrements. Cumulative integrated LAMP1 intensity (relative to the whole cell) was then plotted (Figs 1E–G and EV1A). Curve fitting using nonlinear regress ...
Biological effects of 6 mT static magnetic fields: A comparative study
... Patrizia Tarantino, Bruno Di Jeso, Majdi Dwikat, and Luciana Dini* Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy The present work was a comparative study of the bio-effects induced by exposure to 6 mT static magnetic field (MF) on several primar ...
... Patrizia Tarantino, Bruno Di Jeso, Majdi Dwikat, and Luciana Dini* Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, Italy The present work was a comparative study of the bio-effects induced by exposure to 6 mT static magnetic field (MF) on several primar ...
Dormancy and growth of metastatic breast cancer cells
... The 5 year cure rate for localized breast cancer is high, e.g. 99 % [1]. However, this figure belies the fact that breast cancer can reoccur as metastatic disease many years and even decades after the original treatment [2, 3]. Once relapse occurs, and the cancer colonizes in distant organs, the rel ...
... The 5 year cure rate for localized breast cancer is high, e.g. 99 % [1]. However, this figure belies the fact that breast cancer can reoccur as metastatic disease many years and even decades after the original treatment [2, 3]. Once relapse occurs, and the cancer colonizes in distant organs, the rel ...
ATPase in the plasma membrane of HeLa cells
... RT-PCR to examine the expression of four α-isoforms (α1-α4) and four βisoforms (β1-β4) of Na+/K+-ATPase. Primers used were designed to amplify an approximately 300 bp DNA fragment of each isoform cDNA (for the primer sequences, see supplementary material Table S1). The amplified fragment was subclon ...
... RT-PCR to examine the expression of four α-isoforms (α1-α4) and four βisoforms (β1-β4) of Na+/K+-ATPase. Primers used were designed to amplify an approximately 300 bp DNA fragment of each isoform cDNA (for the primer sequences, see supplementary material Table S1). The amplified fragment was subclon ...
Cytotoxic T Cell Lysis of Target Cells Fused with Liposomes
... Substantial epidemiological and experimental evidence suggests that both T cells and antibodies play a major role in recovery from and protection against influenza virus infection (Schulman, 1975). Anti-haemagglutinin (anti-HA) antibodies are of particular importance since they neutralize the virus; ...
... Substantial epidemiological and experimental evidence suggests that both T cells and antibodies play a major role in recovery from and protection against influenza virus infection (Schulman, 1975). Anti-haemagglutinin (anti-HA) antibodies are of particular importance since they neutralize the virus; ...
PCM-1, A 228-kD Centrosome Autoantigen with a Distinct Cell Cycle
... Fig. 8). Northern blot analysis of total HeLa cell RNA was performed using radioactively labeled cDNA inserts obtained from the two clones and a single mRNA of 7.5-8.0 kb was identified on autoradiographs (Balczon, R., and W. E. Zimmer. 1990. J. Cell Biol. lll:180a). A series of experiments was perf ...
... Fig. 8). Northern blot analysis of total HeLa cell RNA was performed using radioactively labeled cDNA inserts obtained from the two clones and a single mRNA of 7.5-8.0 kb was identified on autoradiographs (Balczon, R., and W. E. Zimmer. 1990. J. Cell Biol. lll:180a). A series of experiments was perf ...
Monocyte-derived dendritic cell generation - HAL
... A growing body of evidence has demonstrated that dendritic cells (DC) are key actors in the diverse facets of immune regulation, including the induction of antitumor immune response (1, 2). Indeed, DC are the most potent antigen-presenting cells. In an immature state, DC exhibit the capacities to ca ...
... A growing body of evidence has demonstrated that dendritic cells (DC) are key actors in the diverse facets of immune regulation, including the induction of antitumor immune response (1, 2). Indeed, DC are the most potent antigen-presenting cells. In an immature state, DC exhibit the capacities to ca ...
The Connexin46 mutant (V44M)
... reticulum (ER) and then transported to the Golgi complex (Musil and Goodenough 1991; Falk et al. 1994). Studies on Cx46 carrying the fs380 mutation (Cx46fs380) showed that mutant connexins were retained within the ER, ERGIC, and/or Golgi apparatus possibly because of misfolding and/or incomplete/imp ...
... reticulum (ER) and then transported to the Golgi complex (Musil and Goodenough 1991; Falk et al. 1994). Studies on Cx46 carrying the fs380 mutation (Cx46fs380) showed that mutant connexins were retained within the ER, ERGIC, and/or Golgi apparatus possibly because of misfolding and/or incomplete/imp ...
Cytokinesis failure occurs in Fanconi anemia pathway
... in DNA replication and repair. The second step of the FA pathway is monoubiquitination of FANCD2 and FANCI by the activated FA core complex (9). Modified FANCD2 and FANCI subsequently accumulate in foci on chromatin, where they colocalize with other repair proteins and participate in DNA interstrand ...
... in DNA replication and repair. The second step of the FA pathway is monoubiquitination of FANCD2 and FANCI by the activated FA core complex (9). Modified FANCD2 and FANCI subsequently accumulate in foci on chromatin, where they colocalize with other repair proteins and participate in DNA interstrand ...
Supplementary Notes - Word file
... represents a polar amino acid (Supplemental Table 2). We also noticed other features that, though not present in all sites, appear enriched in the area surrounding the Cdc5dependent phosphorylation sites. First, an aliphatic amino acid is frequently present in the +1 to +3 region, which has previous ...
... represents a polar amino acid (Supplemental Table 2). We also noticed other features that, though not present in all sites, appear enriched in the area surrounding the Cdc5dependent phosphorylation sites. First, an aliphatic amino acid is frequently present in the +1 to +3 region, which has previous ...
- eScholarship@UMMS
... p53 allowed the binucleates to undergo DNA synthesis. The notion that normal mammalian somatic cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1 after cleavage failure has been intensely attractive for many, including ourselves, because it provides a logical way for an organi ...
... p53 allowed the binucleates to undergo DNA synthesis. The notion that normal mammalian somatic cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1 after cleavage failure has been intensely attractive for many, including ourselves, because it provides a logical way for an organi ...
A High Throughput, Whole Cell Screen for Small
... long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal but relatively stable chromosome content, whereas others exhibit su ...
... long term activation of the spindle checkpoint, whereupon cells arrest at the preanaphase stage for many hours. Many cancers show abnormal chromosome content, often being hyperdiploid. Certain lines derived from tumors have abnormal but relatively stable chromosome content, whereas others exhibit su ...
tetraploidy checkpoint - The Journal of Cell Biology
... p53 allowed the binucleates to undergo DNA synthesis. The notion that normal mammalian somatic cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1 after cleavage failure has been intensely attractive for many, including ourselves, because it provides a logical way for an organi ...
... p53 allowed the binucleates to undergo DNA synthesis. The notion that normal mammalian somatic cells have a “tetraploidy checkpoint” that arrests binucleate cells in G1 after cleavage failure has been intensely attractive for many, including ourselves, because it provides a logical way for an organi ...
Learning about the Importance of Mutation
... ment is involved [27-32], as summarized by Ebos [33]. First, cancer cells recruit normal stromal cells to establish cancer niches, as exemplified by tumor angiogenesis [23]. Second, cancer cells collaborate with each other [1;2;34], which is evident not only in vivo, as in their collective invasion ...
... ment is involved [27-32], as summarized by Ebos [33]. First, cancer cells recruit normal stromal cells to establish cancer niches, as exemplified by tumor angiogenesis [23]. Second, cancer cells collaborate with each other [1;2;34], which is evident not only in vivo, as in their collective invasion ...
The Regulation of Translation in Reovirus-infected Cells
... The reovirus genome comprises ten different pieces of dsRNA, each one coding for a single protein (Joklik, 1981). These different proteins have been classified into groups according to size. Infection of HeLa cells with reovirus type 3 led to massive synthesis of reovirus proteins starting from abou ...
... The reovirus genome comprises ten different pieces of dsRNA, each one coding for a single protein (Joklik, 1981). These different proteins have been classified into groups according to size. Infection of HeLa cells with reovirus type 3 led to massive synthesis of reovirus proteins starting from abou ...
Cell Cycle Phase Specificity and Biochemical
... The plant alkaloid ellipticine [5,1 l-dimethyl-6//-pyrido(4,3-¿>)-carbazole; NSC 71795] has been shown to have significant antitumor activity against L1210 lymphocytic leukemia and other tumor systems in vivo (7, 11). A related compound, 9-methoxyellipticine láclate, produced remissions in patient ...
... The plant alkaloid ellipticine [5,1 l-dimethyl-6//-pyrido(4,3-¿>)-carbazole; NSC 71795] has been shown to have significant antitumor activity against L1210 lymphocytic leukemia and other tumor systems in vivo (7, 11). A related compound, 9-methoxyellipticine láclate, produced remissions in patient ...
Full Text
... because they seem to be secreted into particularly intimate association with extracellular matrix rather than being freely diffusible (Jue et al.. 1992). Wnt-l.secretingcell grafts share with activinsecreting ones the capacity to re-situate streak formation in whole blastoderms (Cooke et al.. in pre ...
... because they seem to be secreted into particularly intimate association with extracellular matrix rather than being freely diffusible (Jue et al.. 1992). Wnt-l.secretingcell grafts share with activinsecreting ones the capacity to re-situate streak formation in whole blastoderms (Cooke et al.. in pre ...
Cell-to-cell Modelling of the inter-phase between Atrial and
... structure conforming the cytoarchitecture of this groups of cells consists of interdigitations of nodal and atrial bundles forming histological connections between nodal and atrial myocytes at regular distances [20]. In [32] the authors, introduce a model of strands of atrial cells penetrating the S ...
... structure conforming the cytoarchitecture of this groups of cells consists of interdigitations of nodal and atrial bundles forming histological connections between nodal and atrial myocytes at regular distances [20]. In [32] the authors, introduce a model of strands of atrial cells penetrating the S ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.