A Mechanism for Asymmetric Cell Division Resulting in Proliferative
... dividing and G0-like cells, while overexpression of AKT2 did not, suggesting that AKT1 is both necessary and sufficient for the production of G0-like cells (Fig. 1B). On the basis of this result, we used site-directed mutagenesis to identify AKT1 domains that might be required for its partial suppres ...
... dividing and G0-like cells, while overexpression of AKT2 did not, suggesting that AKT1 is both necessary and sufficient for the production of G0-like cells (Fig. 1B). On the basis of this result, we used site-directed mutagenesis to identify AKT1 domains that might be required for its partial suppres ...
Functional Complexity Associated with the EspB Molecule of
... truncated EspB constructs seen in the membrane fraction was also detected in the cytosolic fraction (Fig. 1D), a result consistent with the property of translocation. Similarly, the products derived from pB190 and pBd118-190 not found in the membrane fractions of the infected cells were not seen in ...
... truncated EspB constructs seen in the membrane fraction was also detected in the cytosolic fraction (Fig. 1D), a result consistent with the property of translocation. Similarly, the products derived from pB190 and pBd118-190 not found in the membrane fractions of the infected cells were not seen in ...
Changes in Levels of Cellular Constituents in Suspension Culture of
... The level of low-molecular weight phenolic com pounds increased transiently in the cells in both the “+Pi” and the “—Pi” media just after cell transfer. Level of free and incorporated amino acids The free phenolic compounds remained at the initial The level of free amino acids decreased in the cell ...
... The level of low-molecular weight phenolic com pounds increased transiently in the cells in both the “+Pi” and the “—Pi” media just after cell transfer. Level of free and incorporated amino acids The free phenolic compounds remained at the initial The level of free amino acids decreased in the cell ...
Learning About Cells - Carson
... microscopes that allowed him to observe the wonderful world of tiny “animalcules.” These microbes are now known as bacteria and protozoans. The Cell Theory By the 1800s, better microscopes were being made, and scientists had many ideas about cells. Their ideas were put together into a theory, an ide ...
... microscopes that allowed him to observe the wonderful world of tiny “animalcules.” These microbes are now known as bacteria and protozoans. The Cell Theory By the 1800s, better microscopes were being made, and scientists had many ideas about cells. Their ideas were put together into a theory, an ide ...
The three-dimensional arrangement of chromosomes at meiotic
... The precision of the synaptic process and the regularity of chromosome segregation suggests a high degree of order in the eukaryote nucleus prior to and during meiosis. Examples of chromosome order in the nucleus include the regular placement of centromeres and telomeres from telophase through to pr ...
... The precision of the synaptic process and the regularity of chromosome segregation suggests a high degree of order in the eukaryote nucleus prior to and during meiosis. Examples of chromosome order in the nucleus include the regular placement of centromeres and telomeres from telophase through to pr ...
Anti-Invasive Activity of Niacin and Trigonelline against Cancer Cells
... exerted no influence on proliferation. Niacin, trigonelline, and trigonelline-loaded rat serum were found to inhibit ROS-induced elevation of the invasive activity of AH109A cells. In a separate experiment, we analyzed the XO activity by measuring uric acid generated by HX–XO reaction, and nicotinic ...
... exerted no influence on proliferation. Niacin, trigonelline, and trigonelline-loaded rat serum were found to inhibit ROS-induced elevation of the invasive activity of AH109A cells. In a separate experiment, we analyzed the XO activity by measuring uric acid generated by HX–XO reaction, and nicotinic ...
A. Wu, D. Liao, T. Tlsty, J.C. Sturm, R.H. Austin, "Game theory in the death galaxy: interaction of cancer and stromal cells in tumour microenvironment", Interface Focus 4, 20140028 (JUN 2014).
... fibroblasts in melanoma growth and drug resistance from a game-theoretical point of view. However, it is very challenging to assess cancer and non-cancer populations versus time with high temporal resolution in clinical data. Because of the challenge of fitting experimental data, the current game-th ...
... fibroblasts in melanoma growth and drug resistance from a game-theoretical point of view. However, it is very challenging to assess cancer and non-cancer populations versus time with high temporal resolution in clinical data. Because of the challenge of fitting experimental data, the current game-th ...
Fixation and Permeabilization Approaches for Scanning
... close proximity to a substrate.1−4 Thanks to its high spatial resolution and versatility, SECM has been used for the electrochemical imaging and intracellular investigation of different living cell types, including mammalian cells.5−10 Typically, SECM experiments are performed on single cells adheren ...
... close proximity to a substrate.1−4 Thanks to its high spatial resolution and versatility, SECM has been used for the electrochemical imaging and intracellular investigation of different living cell types, including mammalian cells.5−10 Typically, SECM experiments are performed on single cells adheren ...
The Assimilation of Amino-acids by Bacteria
... within the internal environment were released into the external environment and that the nitrogenous material estimated by Hotchkiss might consist partly of such amino-acids. The results reported in this paper show that this is the case (cf. Gale & Taylor, 1946). METHODS Organism and method of cultu ...
... within the internal environment were released into the external environment and that the nitrogenous material estimated by Hotchkiss might consist partly of such amino-acids. The results reported in this paper show that this is the case (cf. Gale & Taylor, 1946). METHODS Organism and method of cultu ...
Effects of herbal preparation EquigaardTM on
... expression.These studiesused prostatecancercells mimicking androgen-independentstate (AXPc), characterized by greater proliferation of cells, lack of responsiveness to androgen the androgen-dependent(AD) and androgen-independent(AI) states of prostate carcinogenesis.Results show that Equiguard block ...
... expression.These studiesused prostatecancercells mimicking androgen-independentstate (AXPc), characterized by greater proliferation of cells, lack of responsiveness to androgen the androgen-dependent(AD) and androgen-independent(AI) states of prostate carcinogenesis.Results show that Equiguard block ...
ab109719 Cell Fractionation Kit - Standard
... for analysis by microplate ELISA or dipstick assay. ab109719 is designed to allow the measurement of any proteins which are differentially represented in the cytosol, mitochondria and nuclei, and is particularly applicable to studies of proteins that translocate between these three cellular compartm ...
... for analysis by microplate ELISA or dipstick assay. ab109719 is designed to allow the measurement of any proteins which are differentially represented in the cytosol, mitochondria and nuclei, and is particularly applicable to studies of proteins that translocate between these three cellular compartm ...
Human TH17 Immune Cells Specific for the
... stage of MAGE-A3–specific TH17, we assessed them in conventional (CD25) CM and EM CD4þ T cells isolated ex vivo by flow cytometry cell sorting (Fig. 2A). Because of the reported relationship between TH17 and Treg (CD25þCD127; ref. 12), we also assessed them in memory Treg (MTreg). MAGE-A3– specific c ...
... stage of MAGE-A3–specific TH17, we assessed them in conventional (CD25) CM and EM CD4þ T cells isolated ex vivo by flow cytometry cell sorting (Fig. 2A). Because of the reported relationship between TH17 and Treg (CD25þCD127; ref. 12), we also assessed them in memory Treg (MTreg). MAGE-A3– specific c ...
Meiosis
... Are cells of organisms the same size? Do your cells get bigger as you grow, or do you just produce more cells? When talking about Cell Size…… Smaller is Better! The larger a cell becomes, the more demands the cell places on its DNA, and the harder it is to supply with nutrients. ...
... Are cells of organisms the same size? Do your cells get bigger as you grow, or do you just produce more cells? When talking about Cell Size…… Smaller is Better! The larger a cell becomes, the more demands the cell places on its DNA, and the harder it is to supply with nutrients. ...
Cell Oxygenation and Cancer
... The Problem for Radiotherapy of Hypoxia in Solid Tumors Although it had been appreciated for several years that lowering the oxygenation of tissues made them more resistant to damage by ionizing radiation (1) , it was the pioneering studies of Gray and colleagues soon after World War II that establi ...
... The Problem for Radiotherapy of Hypoxia in Solid Tumors Although it had been appreciated for several years that lowering the oxygenation of tissues made them more resistant to damage by ionizing radiation (1) , it was the pioneering studies of Gray and colleagues soon after World War II that establi ...
Application of Flow Cytometry Rat Cardiomyocytes
... in a variety of pathological conditions but there is still much debate concerning the nature of its role (4). There is evidence, both in human and animal models, suggesting that apoptosis may be an important mode of cell death during heart failure. The number of apoptotic myocytes is elevated in myo ...
... in a variety of pathological conditions but there is still much debate concerning the nature of its role (4). There is evidence, both in human and animal models, suggesting that apoptosis may be an important mode of cell death during heart failure. The number of apoptotic myocytes is elevated in myo ...
epidermal structure and stomatal ontogeny in some celastraceae
... Mesophyll of all leaves is armed but the form of midrib bundles varies in different species and to some extent in different regions of the same leaf. INTRODUCTION ...
... Mesophyll of all leaves is armed but the form of midrib bundles varies in different species and to some extent in different regions of the same leaf. INTRODUCTION ...
The Production and Action of Interferon in Chinese Hamster Cells
... amounts being produced in 'aged' ceils. Its apparent tool. wt. was 25 ooo. C H O - K t cells, an established line of Chinese hamster cells, did not synthesize interferon in response to viruses, but were sensitive to its action. A functioning interferon system has been discovered in all classes of ve ...
... amounts being produced in 'aged' ceils. Its apparent tool. wt. was 25 ooo. C H O - K t cells, an established line of Chinese hamster cells, did not synthesize interferon in response to viruses, but were sensitive to its action. A functioning interferon system has been discovered in all classes of ve ...
The Effect of Bisphenol A of the Growth of Brest Cancer Cell
... 1. Draw out used medium from flask of MCF-7 cells. 2. Add 5ml of hanks solution to flask and invert so hanks covers all of the bottom of the flask. 3. Remove the hanks solution and then add 2ml of Trypsin and invert so solution covers all the of the bottom of flask. 4. Put the flask of cells into th ...
... 1. Draw out used medium from flask of MCF-7 cells. 2. Add 5ml of hanks solution to flask and invert so hanks covers all of the bottom of the flask. 3. Remove the hanks solution and then add 2ml of Trypsin and invert so solution covers all the of the bottom of flask. 4. Put the flask of cells into th ...
WilliamsPJAS2008
... 1. Draw out used medium from flask of MCF-7 cells. 2. Add 5ml of hanks solution to flask and invert so hanks covers all of the bottom of the flask. 3. Remove the hanks solution and then add 2ml of Trypsin and invert so solution covers all the of the bottom of flask. 4. Put the flask of cells into th ...
... 1. Draw out used medium from flask of MCF-7 cells. 2. Add 5ml of hanks solution to flask and invert so hanks covers all of the bottom of the flask. 3. Remove the hanks solution and then add 2ml of Trypsin and invert so solution covers all the of the bottom of flask. 4. Put the flask of cells into th ...
atp ase-positive and metallophilic cells in the skin of frog, rana
... either the epidermis or dermis. There were occasional lymphocyte-like migrating cells among the epithelial cells of the epidermis, but the Birbeck granules could not be found in these cells. The epithelial cells were connected with each other by desmosomes. Their cytoplasm was abundant in tonofilame ...
... either the epidermis or dermis. There were occasional lymphocyte-like migrating cells among the epithelial cells of the epidermis, but the Birbeck granules could not be found in these cells. The epithelial cells were connected with each other by desmosomes. Their cytoplasm was abundant in tonofilame ...
Nucleic Acid Metabolism in Proliferating and
... Frank Troncale, Ralph Hertz, and Martin Lipkin mucosa were removed at operation from colons that contained a variety of different colon lesions: carcinomas, diverticular disease, a lipoma, and adenomatous polyps. Strips of normal mucosa 1 inch wide and 2 to 3 inches long were taken. The strips were ...
... Frank Troncale, Ralph Hertz, and Martin Lipkin mucosa were removed at operation from colons that contained a variety of different colon lesions: carcinomas, diverticular disease, a lipoma, and adenomatous polyps. Strips of normal mucosa 1 inch wide and 2 to 3 inches long were taken. The strips were ...
DF-Bio714-2
... If TGF- signaling is suppressed or defective, there is no G1 arrest with rapamycin treatment - and now the cells die in the presence of rapamycin - Why? ...
... If TGF- signaling is suppressed or defective, there is no G1 arrest with rapamycin treatment - and now the cells die in the presence of rapamycin - Why? ...
Cell Lines
... • Some of these cell lines have actually been derived from tumors or are transformed spontaneously in culture by mutations • Chemical or gamma ray treated cells can become infinite with loss of growth factors • Viral infection with SV40 T antigen can insert oncogenes and lead to gene alteration • No ...
... • Some of these cell lines have actually been derived from tumors or are transformed spontaneously in culture by mutations • Chemical or gamma ray treated cells can become infinite with loss of growth factors • Viral infection with SV40 T antigen can insert oncogenes and lead to gene alteration • No ...
Identification of trichoplein, a novel keratin filament
... according to the manufacturer’s protocol (Qiagen). The C-terminal region of trichoplein was expressed as glutathione S-transferase (GST) fusion protein in E. coli and purified on glutathione-agarose beads, essentially as described (Smith and Johnson, 1988). The expression and purification of His6-ta ...
... according to the manufacturer’s protocol (Qiagen). The C-terminal region of trichoplein was expressed as glutathione S-transferase (GST) fusion protein in E. coli and purified on glutathione-agarose beads, essentially as described (Smith and Johnson, 1988). The expression and purification of His6-ta ...
Plasma Membrane Lipid Structural Order in Doxorubicin
... Several studies have indicated that intracellular drug accu mulation is decreased in resistant lines, both for the drug used to prepare the resistant subline and for drugs to which the subline is cross-resistant. (2, 4, 8, 17, 18, 28, 29, 32, 39, 40). It is not clear whether this decreased drug accu ...
... Several studies have indicated that intracellular drug accu mulation is decreased in resistant lines, both for the drug used to prepare the resistant subline and for drugs to which the subline is cross-resistant. (2, 4, 8, 17, 18, 28, 29, 32, 39, 40). It is not clear whether this decreased drug accu ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.