Supporting Information Fredericks et al. 10.1073/pnas.1417176111
... protein compared with nontargeting control shRNA. Clones that were found to have targets effectively knocked down were then used for functional tests. Western Blots, Immunoprecipitations, and Real-Time PCR For all experiments involving BMDMs, cells were plated in complete media for 5 d after isolati ...
... protein compared with nontargeting control shRNA. Clones that were found to have targets effectively knocked down were then used for functional tests. Western Blots, Immunoprecipitations, and Real-Time PCR For all experiments involving BMDMs, cells were plated in complete media for 5 d after isolati ...
Morphological Basis for the Cytolytic Effect of
... inward shift of Ca@ during mitosis is responsible not only for the breakdown of this differential but also possibly leads to chromosomal condensation ( I 3). In view of observations showing that vinblastine in higher concentrations acts like Ca@@and precipitates a number of cellular proteins as well ...
... inward shift of Ca@ during mitosis is responsible not only for the breakdown of this differential but also possibly leads to chromosomal condensation ( I 3). In view of observations showing that vinblastine in higher concentrations acts like Ca@@and precipitates a number of cellular proteins as well ...
repp86: A Human Protein Associated in the Progression of Mitosis
... mAb was obtained, which stained the same pattern as mAb Ki-S2 in immunohistochemistry. This antibody also recognized immunopurified repp86 in Western blot experiments, indicating that the elicited cDNA actually encodes repp86. Screening of a genomic data bank with the cDNA sequence of repp86 led to ...
... mAb was obtained, which stained the same pattern as mAb Ki-S2 in immunohistochemistry. This antibody also recognized immunopurified repp86 in Western blot experiments, indicating that the elicited cDNA actually encodes repp86. Screening of a genomic data bank with the cDNA sequence of repp86 led to ...
APPLICATION NOTE
... of autophagic response, but different magnitude of fluorescence signal increases, which may arise from different analysis approaches characteristic of the two instrument platforms. The possibility of using this method for drug discovery applications is also demonstrated through the measurement of do ...
... of autophagic response, but different magnitude of fluorescence signal increases, which may arise from different analysis approaches characteristic of the two instrument platforms. The possibility of using this method for drug discovery applications is also demonstrated through the measurement of do ...
Tracking of Cells in a Sequence of Images Using a Low
... of the process by a factor typically equal to 10. Both steps use no intensity-based thresholds or parameters, but geometric cell properties (minimal and maximal width, minimal area, etc.) and simple parametric cell motion priors. We show experiments made on real data and comment the results obtained ...
... of the process by a factor typically equal to 10. Both steps use no intensity-based thresholds or parameters, but geometric cell properties (minimal and maximal width, minimal area, etc.) and simple parametric cell motion priors. We show experiments made on real data and comment the results obtained ...
The Surface Ultrastructure of Normal and
... (1, 2, 5) established the fact that the intercellular adhesiveness of malignant cells was lower than that of their normal prototypes and that this difference probably played a major role in the invasive characteristics of cancer cells. Since ad hesiveness is a surface phenomenon, it seemed reasonabl ...
... (1, 2, 5) established the fact that the intercellular adhesiveness of malignant cells was lower than that of their normal prototypes and that this difference probably played a major role in the invasive characteristics of cancer cells. Since ad hesiveness is a surface phenomenon, it seemed reasonabl ...
Primary Cell Cultures from Drosophila Gastrula Embryos
... The developmental pattern of primary cultures of Drosophila cells can vary greatly, possibly due to the several variables during the primary cell preparation process. First of all, flies used for egg laying should be young (within a week old) and healthy (free of viral or bacterial infection). Unfer ...
... The developmental pattern of primary cultures of Drosophila cells can vary greatly, possibly due to the several variables during the primary cell preparation process. First of all, flies used for egg laying should be young (within a week old) and healthy (free of viral or bacterial infection). Unfer ...
Clicker review w/answers
... A They are found in animal cells but not in plant cells B They have genes for the same traits at the same loci. C They pair up in prophase II D They are found in haploid cells E They are found in the cells of human females but not in human males. 3 When we say that an organism is haploid, we mean th ...
... A They are found in animal cells but not in plant cells B They have genes for the same traits at the same loci. C They pair up in prophase II D They are found in haploid cells E They are found in the cells of human females but not in human males. 3 When we say that an organism is haploid, we mean th ...
3.1 Cells and cell function - Pearson Schools and FE Colleges
... background image with boxes – remove one per slide). Play in teams. Individual learners race to name each picture. Their team then provides a description of how the cell is specialised. Stretch and support Ensure learners work in mixed-ability groups of three during the practical activity. Less able ...
... background image with boxes – remove one per slide). Play in teams. Individual learners race to name each picture. Their team then provides a description of how the cell is specialised. Stretch and support Ensure learners work in mixed-ability groups of three during the practical activity. Less able ...
Environmentally Controlled Invasion of Cancer Cells by Engineered
... monocytogenes.20,40 This occurs for a wide variety of solid tumors, including bladder, brain, and breast cancers.40 These microbes impart their selectivity for tumors by exploiting the hypoxic microenvironment, poor immune surveillance, and the increased availability of nutrients.41,21,22 After a Sa ...
... monocytogenes.20,40 This occurs for a wide variety of solid tumors, including bladder, brain, and breast cancers.40 These microbes impart their selectivity for tumors by exploiting the hypoxic microenvironment, poor immune surveillance, and the increased availability of nutrients.41,21,22 After a Sa ...
CELL DIVISION Mitosis
... Copyright © 2004 Pearson Education, Inc. publishing as Benjamin Cummings ...
... Copyright © 2004 Pearson Education, Inc. publishing as Benjamin Cummings ...
A Method for Producing, Selecting, and Isolating
... mutants should be considered. As described previously (18), DCMU-treated cells contained a reduced average number of chloroplasts. What seems even more important is the observation that a substantial number of these cells contained only one chloroplast. Obviously these cells were the best candidates ...
... mutants should be considered. As described previously (18), DCMU-treated cells contained a reduced average number of chloroplasts. What seems even more important is the observation that a substantial number of these cells contained only one chloroplast. Obviously these cells were the best candidates ...
Jordanian Ducrosia flabellifolia inhibits proliferation of breast cancer
... evaluated in this study. The antiproliferative activity was tested against three cell lines using MTT assay. The apoptosis induction ability was determined using TUNEL colorimetric assay and agarose gel electrophoresis was used to detect DNA fragmentation. Morphological changes associated with apopt ...
... evaluated in this study. The antiproliferative activity was tested against three cell lines using MTT assay. The apoptosis induction ability was determined using TUNEL colorimetric assay and agarose gel electrophoresis was used to detect DNA fragmentation. Morphological changes associated with apopt ...
Gossypol Induced Cell Death in DU 145 Prostate Cancer Cells
... This Thesis is brought to you for free and open access by the Theses and Dissertations at e-publications@RCSI. It has been accepted for inclusion in MSc by research theses by an authorized administrator of epublications@RCSI. For more information, please contact [email protected]. ...
... This Thesis is brought to you for free and open access by the Theses and Dissertations at e-publications@RCSI. It has been accepted for inclusion in MSc by research theses by an authorized administrator of epublications@RCSI. For more information, please contact [email protected]. ...
The BALB/c 3T3 cell transformation assay to assess the
... Cell transformation assays (CTAs) have been proposed as screening tests for the carcinogenic potential of compounds that have no evidence of genotoxicity and are listed among the REACH methods, as reported in EU regulation 440/2008. ...
... Cell transformation assays (CTAs) have been proposed as screening tests for the carcinogenic potential of compounds that have no evidence of genotoxicity and are listed among the REACH methods, as reported in EU regulation 440/2008. ...
Electron microscopic studies of the corpuscles of Stannius of an
... The corpuscles of Stannius (CS) are tiny endocrine glands located on the kidneys of holostean and teleostean fishes. The CS are involved in calcium homeostasis by a calcium lowering hormone, stanniocalcin, a homodimeric glycoprotein. The stanniocalcin effectively reduces the uptake of calcium from t ...
... The corpuscles of Stannius (CS) are tiny endocrine glands located on the kidneys of holostean and teleostean fishes. The CS are involved in calcium homeostasis by a calcium lowering hormone, stanniocalcin, a homodimeric glycoprotein. The stanniocalcin effectively reduces the uptake of calcium from t ...
" Avacuolate" Cell in Cortical Tissue of Woody Plant with Special
... nearly the same degree as the normal ones in a hypertonic balanced salt solution, despite o~ the rupture of their tonoplasts. As to the osmotic concentration of the cel[ content, there was only a slight difference between the normal and abnormal cells (Figs. 10-12): the osmotic value in normal cells ...
... nearly the same degree as the normal ones in a hypertonic balanced salt solution, despite o~ the rupture of their tonoplasts. As to the osmotic concentration of the cel[ content, there was only a slight difference between the normal and abnormal cells (Figs. 10-12): the osmotic value in normal cells ...
Supplemental Materials
... cells from [pIC]PEI-induced apoptosis. Pancreatic cancer cells treated with the indicated doses of [pIC]PEI for 24 hours and then treated with the MG132 proteasomal inhibitor for an additional 24 hours. (A) Cells were collected and Western blotting was done for XIAP, LC3, and PARP. Actin served as ...
... cells from [pIC]PEI-induced apoptosis. Pancreatic cancer cells treated with the indicated doses of [pIC]PEI for 24 hours and then treated with the MG132 proteasomal inhibitor for an additional 24 hours. (A) Cells were collected and Western blotting was done for XIAP, LC3, and PARP. Actin served as ...
2.1 Plant and Animal Cells pg. 29 Biology – The study of living
... Interphase: is the phase of the cell cycle during which the cell performs its normal functions and its genetic material is copied in preparation for cell division. It is the longest stage of the cell cycle, and performs all the life functions; growth, cellular reproduction and specialized functions. ...
... Interphase: is the phase of the cell cycle during which the cell performs its normal functions and its genetic material is copied in preparation for cell division. It is the longest stage of the cell cycle, and performs all the life functions; growth, cellular reproduction and specialized functions. ...
Culturing and Fluorescent Staining of B16 Melanoma Cells
... of B16 Melanoma Cells In this lab exercise you will learn how animal cells can be grown in culture and used to analyze the cytosolic arrangement of actin filaments. Cell culture is a widely used methodology for studying the behavior of cells independent of the variables existing within the whole org ...
... of B16 Melanoma Cells In this lab exercise you will learn how animal cells can be grown in culture and used to analyze the cytosolic arrangement of actin filaments. Cell culture is a widely used methodology for studying the behavior of cells independent of the variables existing within the whole org ...
Gene Section DDIT4 (DNA-damage-inducible transcript 4) Atlas of Genetics and Cytogenetics
... REDD1 crystal structure (PDB ID: 3LQ9). A) Cartoon diagram of REDD1 (89-226∆200-204) structure colored in rainbow mode from Nto C- terminus. Dotted line represents a disordered region. B) REDD1 sequence alignment in different species colored in blue gradient according to conservation. Red boxes indi ...
... REDD1 crystal structure (PDB ID: 3LQ9). A) Cartoon diagram of REDD1 (89-226∆200-204) structure colored in rainbow mode from Nto C- terminus. Dotted line represents a disordered region. B) REDD1 sequence alignment in different species colored in blue gradient according to conservation. Red boxes indi ...
Wormwood is the basis for a cancer-fighting pill
... The next step, according to Lai, is animal testing. Limited tests have been done in that area. In an earlier study, a dog with bone cancer so severe it couldn't walk made a complete recovery in five days after receiving the treatment. But more rigorous testing is needed. If the process lives up to i ...
... The next step, according to Lai, is animal testing. Limited tests have been done in that area. In an earlier study, a dog with bone cancer so severe it couldn't walk made a complete recovery in five days after receiving the treatment. But more rigorous testing is needed. If the process lives up to i ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.