Download Technical data sheet

Version: 1
Revision Date: 11/30/2012
Purified anti-p97/VCP Antibody
Catalog# / Size
636802 / 100 µg
636801 / 25 µg
Clone
4G9 (See other available formats)
Other Names
Transitional endoplasmic reticulum ATPase, p97, TERA
Isotype
Mouse IgG1, κ
Ave. Rating
★★★★★ 0 reviews
Description
p97/VCP (valosin containing protein) belongs to the AAA ATPase family,
predicted molecular weight 97 kD. VCP is associated with a variety of
cellular activities, including cell cycle control, membrane fusion after
mitosis, regulates spindle disassembly at the end of mitosis, nuclear
trafficking and the ubiquitin-proteasome degradation pathway. In many
instances, specific adaptor proteins with which VCP forms multiprotein
complexes direct its role in a given process. This protein is required for the
fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful
for western blotting, immunoprecipitation, and immunofluorescence staining
of human p97/VCP protein.
Overnight nocodazole treated Hela cells
stained with purified mouse monoclonal
antibody against p97/VCP (clone 4G9),
followed by Cy3 conjugated goat anti-mouse
IgG and DAPI
Western blot analysis of extracts from Hela
cells (Lane 1), Jurkat cells (Lane 2), MCF7
cells (Lane 3), or mouse splenocytes (Lane
4), using anti-p97/VCP, clone 4G9
Reactivity
Human
Immunogen
Recombinant human valosin containing protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium
azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C."
Application
WB - Quality tested
IP, IF - These applications have been reported by the developer
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting,
suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For
immunofluorescence microscopy: Use a dilution range of 1~4 µg/ml. It is recommended that the
reagent be titrated for optimal performance for each application.
Application References
1. Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed.
2. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed.
3. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed.
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_2214641 (BioLegend Cat. No. 636802)
AB_2257136 (BioLegend Cat. No. 636801)
Antigen Details
Structure
Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm
diameter and displays 6-fold radial symmetry.
Interaction
VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP
Bioactivity
VCP is associated with a variety of cellular activities, including cell cycle control, membrane
fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and
the ubiquitin-proteasome degradation pathway.
Antigen References
1. Ye Y, et al. 2001. Nature 414:652.
Gene ID
7415
UniProt
View information about p97/VCP on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Western Blotting Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Antibodies and More > Cell Biology > Antigens A-Z > p97/VCP >
Antibodies and More > Cell Biology > Cell Cycle/DNA Replication > p97/VCP >
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or
other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or
used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties
without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited
Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or
animal diagnostic, therapeutic or commercial use.
BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587