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Version: 1 Revision Date: 11/30/2012 Purified anti-p97/VCP Antibody Catalog# / Size 636802 / 100 µg 636801 / 25 µg Clone 4G9 (See other available formats) Other Names Transitional endoplasmic reticulum ATPase, p97, TERA Isotype Mouse IgG1, κ Ave. Rating ★★★★★ 0 reviews Description p97/VCP (valosin containing protein) belongs to the AAA ATPase family, predicted molecular weight 97 kD. VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. In many instances, specific adaptor proteins with which VCP forms multiprotein complexes direct its role in a given process. This protein is required for the fusion of ER and Golgi membranes. Clone 4G9 has been shown to be useful for western blotting, immunoprecipitation, and immunofluorescence staining of human p97/VCP protein. Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI Western blot analysis of extracts from Hela cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9 Reactivity Human Immunogen Recombinant human valosin containing protein Formulation This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml. Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml Storage & Handling Upon receipt, store undiluted between 2°C and 8°C." Application WB - Quality tested IP, IF - These applications have been reported by the developer Recommended Usage Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application. Application References 1. Shchedrina VA, et al. 2011. J Biol Chem. 186:42937. PubMed. 2. Yi L, et al. 2012. Hum Mol Genet. 21:1794. PubMed. 3. Yahiro K, et al. 2012. Infect Immun. 80:1803. PubMed. (PubMed link indicates BioLegend citation) Publication Library RRID AB_2214641 (BioLegend Cat. No. 636802) AB_2257136 (BioLegend Cat. No. 636801) Antigen Details Structure Belongs to the AAA ATPase family, homohexamer, forms a ring-shaped particle of 12.5 nm diameter and displays 6-fold radial symmetry. Interaction VCIP135, SELS/VIMP, SYVN1, DERL1, DERL2, DERL3, SVIP Bioactivity VCP is associated with a variety of cellular activities, including cell cycle control, membrane fusion after mitosis, regulates spindle disassembly at the end of mitosis, nuclear trafficking and the ubiquitin-proteasome degradation pathway. Antigen References 1. Ye Y, et al. 2001. Nature 414:652. Gene ID 7415 UniProt View information about p97/VCP on UniProt.org Documentation Technical data sheet Certificate of Analysis Safety Data Sheet Related Protocols Western Blotting Protocol Related FAQs There are no FAQs for this product. Related Categories Antibodies and More > Cell Biology > Antigens A-Z > p97/VCP > Antibodies and More > Cell Biology > Cell Cycle/DNA Replication > p97/VCP > For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use. BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587