1 Distinct FAK activities determine progenitor
... reconstitute a functional mammary gland by generating lineage-restricted progenitor cells, as shown in transplantation studies (2, 3, 6). By contrast, recent lineage-tracing experiments have alternatively proposed that distinct unipotent MaSC populations, located in the luminal and basal compartment ...
... reconstitute a functional mammary gland by generating lineage-restricted progenitor cells, as shown in transplantation studies (2, 3, 6). By contrast, recent lineage-tracing experiments have alternatively proposed that distinct unipotent MaSC populations, located in the luminal and basal compartment ...
Copyright Information of the Article Published Online
... resistance[4]. Accumulating evidence indicates that a subset of cancer cells with high self-renewal and stemness properties, known as cancer stem cells (CSCs), are the key contributors to chemo-resistance, and are responsible for tumor progression and recurrence after conventional therapy[5]. CD44, ...
... resistance[4]. Accumulating evidence indicates that a subset of cancer cells with high self-renewal and stemness properties, known as cancer stem cells (CSCs), are the key contributors to chemo-resistance, and are responsible for tumor progression and recurrence after conventional therapy[5]. CD44, ...
Growth and killing of a Salmonella enterica serovar
... strains), as described previously (Beuzo! n et al., 2000). Spleens were removed aseptically 3 days after inoculation, and placed in 2 ml ice-cold PBS. Cell suspensions were obtained as described previously (Salcedo et al., 2001). Briefly, cell suspensions were obtained by gentle mechanical disruptio ...
... strains), as described previously (Beuzo! n et al., 2000). Spleens were removed aseptically 3 days after inoculation, and placed in 2 ml ice-cold PBS. Cell suspensions were obtained as described previously (Salcedo et al., 2001). Briefly, cell suspensions were obtained by gentle mechanical disruptio ...
Activation of clinically used anthracyclines by the formaldehyde
... neoplastic diseases. The major clinically used anthracyclines consist of doxorubicin, daunorubicin, idarubicin, and epirubicin. Doxorubicin is a broad-spectrum antitumor antibiotic, and the tumors most commonly responsive to this drug include breast and esophageal carcinomas, osteosarcoma, soft tiss ...
... neoplastic diseases. The major clinically used anthracyclines consist of doxorubicin, daunorubicin, idarubicin, and epirubicin. Doxorubicin is a broad-spectrum antitumor antibiotic, and the tumors most commonly responsive to this drug include breast and esophageal carcinomas, osteosarcoma, soft tiss ...
Reprogramming glucose metabolism in cancer: can it be
... cancer metabolism in general, were not considered to be major branches of cancer biology. However, in the past 15 years, there has been a growing interest in cancer metabolism, particularly glucose metabolism in cancer cells; these topics have now become an integral part of cancer biology, similarly ...
... cancer metabolism in general, were not considered to be major branches of cancer biology. However, in the past 15 years, there has been a growing interest in cancer metabolism, particularly glucose metabolism in cancer cells; these topics have now become an integral part of cancer biology, similarly ...
negative Drug-resistant Cell Lines Using Laser
... persistence in cell numbers over 48 h even in 10~5M drug. This agrees with our previously published values for the 50% inhib itory concentration of DNR by clonogenic assay of 0.05 MMfor sensitive and 2.5 /UMfor resistant cells (9). Comparison of drug localization in sensitive and resistant cells at ...
... persistence in cell numbers over 48 h even in 10~5M drug. This agrees with our previously published values for the 50% inhib itory concentration of DNR by clonogenic assay of 0.05 MMfor sensitive and 2.5 /UMfor resistant cells (9). Comparison of drug localization in sensitive and resistant cells at ...
Adipocyte lipolysis links obesity to breast cancer growth: adipocyte
... highly relevant in the breast where adipocytes, professional lipid storage cells, are the predominant cell population and are capable of secreting significant quantities of metabolic substrates such as glycerol and fatty acids. Further, there is close juxtaposition of adipocytes and breast cancer ce ...
... highly relevant in the breast where adipocytes, professional lipid storage cells, are the predominant cell population and are capable of secreting significant quantities of metabolic substrates such as glycerol and fatty acids. Further, there is close juxtaposition of adipocytes and breast cancer ce ...
Uptake of glutamate, not glutamine synthetase, regulates adaptation
... development of a glutamine-free medium which could support cell growth would be attractive. One approach to the formulation of a glutamine-free medium is to utilise a glutamine analogue which is less ammoniagenic. There are numerous reports of the ability of high concentrations (20 mM) of glutamic a ...
... development of a glutamine-free medium which could support cell growth would be attractive. One approach to the formulation of a glutamine-free medium is to utilise a glutamine analogue which is less ammoniagenic. There are numerous reports of the ability of high concentrations (20 mM) of glutamic a ...
DDX3 functions in antiviral innate immunity through translational
... It has emerged that DDX3 plays a role in antiviral innate immunity. However, the exact mechanism by which DDX3 functions in antiviral innate immunity remains to be determined. We found that the expression of the protein activator of the interferon-induced protein kinase (PACT) was regulated by DDX3 ...
... It has emerged that DDX3 plays a role in antiviral innate immunity. However, the exact mechanism by which DDX3 functions in antiviral innate immunity remains to be determined. We found that the expression of the protein activator of the interferon-induced protein kinase (PACT) was regulated by DDX3 ...
Acidotropic probes and flow cytometry: a powerful combination for
... on Rhodomonas salina. The maximum ingestion rate of O. marina was 1.7 prey ind.–1 h–1, and the frequency of cells with R. salina in the food vacuoles increased from 0 to 2.4 ± 0.5 × 103 cells ml–1 within 6 h. The blue probe stained 100% of O. marina cells that had R. salina in the food vacuoles. The ...
... on Rhodomonas salina. The maximum ingestion rate of O. marina was 1.7 prey ind.–1 h–1, and the frequency of cells with R. salina in the food vacuoles increased from 0 to 2.4 ± 0.5 × 103 cells ml–1 within 6 h. The blue probe stained 100% of O. marina cells that had R. salina in the food vacuoles. The ...
Bellairs pm6.5 - The International Journal of Developmental Biology
... substratum cells migrated forming a halo. This useful fact had long been known to investigators studying cells in tissue culture, but Abercrombie’s genius lay in his exploitation of the technique. The cells that migrate out are known as fibroblasts, but typically, in his 1976 paper with Dunn and Hea ...
... substratum cells migrated forming a halo. This useful fact had long been known to investigators studying cells in tissue culture, but Abercrombie’s genius lay in his exploitation of the technique. The cells that migrate out are known as fibroblasts, but typically, in his 1976 paper with Dunn and Hea ...
fulltext - DiVA Portal
... levels and nature of the myriad of biomolecules present. Aberrantly regulated or damaged genes can cause pathological states due to disruption of various biological processes [1,2]. In some instances, these genetic aberrations are inherited insofar as they are present in the germline cells but in ot ...
... levels and nature of the myriad of biomolecules present. Aberrantly regulated or damaged genes can cause pathological states due to disruption of various biological processes [1,2]. In some instances, these genetic aberrations are inherited insofar as they are present in the germline cells but in ot ...
jaf op den kamp*, w. van - Utrecht University Repository
... due to an increase in the formation of fl-hydroxybutyric acid as demonstrated b y LEMOmNE is and confirmed by the electron micrographs showing the presence of more granules in cells from medium B (Figs. io and I3). The present data do not allow conclusions to be made about the amount of phospholipid ...
... due to an increase in the formation of fl-hydroxybutyric acid as demonstrated b y LEMOmNE is and confirmed by the electron micrographs showing the presence of more granules in cells from medium B (Figs. io and I3). The present data do not allow conclusions to be made about the amount of phospholipid ...
PGC specification from epiblast
... Primordial germ cells (PGCs) in mice have been recognized histologically as alkaline phosphatase (AP) activity-positive cells at 7.2 days post coitum (dpc) in the extra-embryonic mesoderm. However, mechanisms regulating PGC formation are unknown, and an appropriate in vitro system to study the mecha ...
... Primordial germ cells (PGCs) in mice have been recognized histologically as alkaline phosphatase (AP) activity-positive cells at 7.2 days post coitum (dpc) in the extra-embryonic mesoderm. However, mechanisms regulating PGC formation are unknown, and an appropriate in vitro system to study the mecha ...
Saquinavir, an HIV Protease Inhibitor, Is Transported by P
... and advanced-stage HIV patients. Saquinavir mesylate (Invirase, originally Ro 31-8959) is one such agent and was the first to become clinically available in the United States to HIV patients (fig. 1). In infected cells, the integrated HIV viral DNA is translated into a polyprotein that requires clea ...
... and advanced-stage HIV patients. Saquinavir mesylate (Invirase, originally Ro 31-8959) is one such agent and was the first to become clinically available in the United States to HIV patients (fig. 1). In infected cells, the integrated HIV viral DNA is translated into a polyprotein that requires clea ...
Autocrine Effects of Fibroblast Growth Factor in Repair of Radiation
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
... cycle analysis by flow cytometry showed that 91 ±1.13% (mean ±SE of 6 experiments) of the cells were in G0-G| phase, 3.66% ±0.71% in S phase, and 5.33 ±0.66% in G2-M phase. Fig. 1 shows the dose-survival curves generated with these cells. Im mediately after irradiation the cells were seeded on e ...
Merotelic kinetochore orientation occurs frequently during early
... *Author for correspondence (e-mail: [email protected]) ...
... *Author for correspondence (e-mail: [email protected]) ...
separation of cell types from embryonic chicken and rat spinal cord
... P. Daniels in whose laboratories the work was completed. We also wish to acknowledge Ms. Lisa Chang for aid in preparing the micrographs, Dr. Mary Ellen Michel for aid in tetanus toxin histochemistry, and Mrs. Mary Riggin for manuscript preparation. ‘To whom all correspondence should be directed at ...
... P. Daniels in whose laboratories the work was completed. We also wish to acknowledge Ms. Lisa Chang for aid in preparing the micrographs, Dr. Mary Ellen Michel for aid in tetanus toxin histochemistry, and Mrs. Mary Riggin for manuscript preparation. ‘To whom all correspondence should be directed at ...
The effective nuclear delivery of doxorubicin from dextran
... solution was added to the synthesized 4 mL of dAuNPs. We reduced amount of NaOH for 170 nm dAuNPs to maintain colloidal stability during the reaction. The reaction mixture was vortexed vigorously for 10 s and then, 100 mL of epichlorohydrin was added to the mixture to make 2.5 v/v% mixture. Mixed so ...
... solution was added to the synthesized 4 mL of dAuNPs. We reduced amount of NaOH for 170 nm dAuNPs to maintain colloidal stability during the reaction. The reaction mixture was vortexed vigorously for 10 s and then, 100 mL of epichlorohydrin was added to the mixture to make 2.5 v/v% mixture. Mixed so ...
Frost Resistance in Algae Cells
... Upon freezing the cells in 1 M glucose solution were extremely dehydrated and flattened, the center part of filamentous cells became slender, but no plasmolysis occurred at all, in these cells. When thawed, the protoplast shrank and coagulated, and pseudoplasmolysis was observed in a part of the cel ...
... Upon freezing the cells in 1 M glucose solution were extremely dehydrated and flattened, the center part of filamentous cells became slender, but no plasmolysis occurred at all, in these cells. When thawed, the protoplast shrank and coagulated, and pseudoplasmolysis was observed in a part of the cel ...
COMPOUND-INDUCED HOST CELL RESPONSES AND EFFECTS ON WHOLE VIRUS CHAPTER 4
... determining ADMET properties in vitro. Brain microvessel endothelial cells have been used in BBB penetration studies (Glynn and Yazdanian, 1998) and Caco-2 cells for cellular permeability (Egan and Lauri, 2002). Because cytotoxicity is one of the most critical and unpredictable of the drug-like prop ...
... determining ADMET properties in vitro. Brain microvessel endothelial cells have been used in BBB penetration studies (Glynn and Yazdanian, 1998) and Caco-2 cells for cellular permeability (Egan and Lauri, 2002). Because cytotoxicity is one of the most critical and unpredictable of the drug-like prop ...
Galectin-3 Cleavage Alters Bone Remodeling: Different Outcomes in
... in bone, resulting in pain (2). Thus, unlike other metastatic sites, anticancer therapeutic approaches alone are not enough to treat bone metastasis and alleviate pain (17, 18). Evidence from a clinical study showed that it is significant to control not only cancer growth but also osteoclasts in meta ...
... in bone, resulting in pain (2). Thus, unlike other metastatic sites, anticancer therapeutic approaches alone are not enough to treat bone metastasis and alleviate pain (17, 18). Evidence from a clinical study showed that it is significant to control not only cancer growth but also osteoclasts in meta ...
Cancer-Initiating Cells from Colorectal Cancer Patients Escape from
... progression, and resistance to therapeutic agents (4–6); therefore, treatments aimed at blocking/destroying CICs should be pursued. Despite the advent of new diagnostic tools and targeted molecule agents, most CRC patients die of metastatic disease and almost half of patients receive chemotherapy wi ...
... progression, and resistance to therapeutic agents (4–6); therefore, treatments aimed at blocking/destroying CICs should be pursued. Despite the advent of new diagnostic tools and targeted molecule agents, most CRC patients die of metastatic disease and almost half of patients receive chemotherapy wi ...
Lysosomal Sequestration of Polyamine
... trials—despite accumulating ~5 times more analogue than parental cells. We now report that treatment of CHO/664 cells with DENSPM results in the formation of numerous large cytoplasmic vacuoles, which on the basis of electron microscopy and cytochemical staining seem to be lysosomal in origin. A s ...
... trials—despite accumulating ~5 times more analogue than parental cells. We now report that treatment of CHO/664 cells with DENSPM results in the formation of numerous large cytoplasmic vacuoles, which on the basis of electron microscopy and cytochemical staining seem to be lysosomal in origin. A s ...
Mouse mammary tumor virus-based vector transduces non
... mammary gland. It is not clear how the prototypic betaretrovirus infects mucosal DCs and naïve lymphocytes as these cells are considered to be non-proliferative. Studies of MMTV biology have been hampered by the difficulty of obtaining sufficient virus/vector titers after transfection of a molecular ...
... mammary gland. It is not clear how the prototypic betaretrovirus infects mucosal DCs and naïve lymphocytes as these cells are considered to be non-proliferative. Studies of MMTV biology have been hampered by the difficulty of obtaining sufficient virus/vector titers after transfection of a molecular ...
HeLa
A HeLa cell /ˈhiːlɑː/, also Hela or hela cell, is a cell type in an immortal cell line used in scientific research. It is the oldest and most commonly used human cell line. The line was derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a patient who eventually died of her cancer on October 4, 1951. The cell line was found to be remarkably durable and prolific — which has led to its contamination of many other cell lines used in research.The cells from Lacks's tumor were taken without her knowledge or consent by researcher George Gey, who found that they could be kept alive. Before this, cells cultured from other cells would only survive for a few days. Scientists spent more time trying to keep the cells alive than performing actual research on the cells, but some cells from Lacks's tumor sample behaved differently from others. George Gey was able to isolate one specific cell, multiply it, and start a cell line. Gey named the sample HeLa, after the initial letters of Henrietta Lacks' name. As the first human cells grown in a lab that were ""immortal"" (they do not die after a few cell divisions), they could be used for conducting many experiments. This represented an enormous boon to medical and biological research.The stable growth of HeLa enabled a researcher at the University of Minnesota hospital to successfully grow polio virus, enabling the development of a vaccine. By 1954 Jonas Salk developed a vaccine for polio using these cells. To test Salk's new vaccine, the cells were quickly put into mass production in the first-ever cell production factory.In 1955 HeLa cells were the first human cells successfully cloned.Demand for the HeLa cells quickly grew. Since they were put into mass production, Lacks's cells have been used by scientists around the globe for ""research into cancer, AIDS, the effects of radiation and toxic substances, gene mapping, and countless other scientific pursuits"". HeLa cells have been used to test human sensitivity to tape, glue, cosmetics, and many other products. Scientists have grown some 20 tons of her cells, and there are almost 11,000 patents involving HeLa cells.