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Transcript
PROGRAMMEDESTAGED’INITIATIONALARECHERCHE AU1ERCYCLE
SIRI
UniversitédeLyon/Universitéd’Ottawa
Appelàcandidature2017
Title
Encodingtimeinfearmemories
SurnameMOULY
Supervisor-s
FirstNameAnne-Marie
[email protected]
Phone+33(0)437287493
Institute
Lab
Context
Abstract/Objectives
SurnamePEYRON
FirstNameChristelle
[email protected]
Phone+33(0)478771042
LyonNeuroscienceResearchCenter,
CNRSUMR5292-INSERMU1028-UniversitéLyon1
https://crnl.univ-lyon1.fr/index.php/en/Research/CRNL-teams-20162020
TeamCMO
https://crnl.univ-lyon1.fr/index.php/en/Research/CRNL-teams-20162020/19
TeamSLEEP
https://crnl.univ-lyon1.fr/index.php/en/Research/CRNL-teams-20162020/17
How fear memories are formed and retrieved remains one of the most
important questions in neuroscience, in part because stress and anxiety
pathologies are continuously increasing in our modern society. In
animals, fear memories can be assessed through a very popular
paradigm,fearconditioning.Inthistask,asensorystimulus(forexample
anodor)ispresentedtotheanimalandafterafixedinterval(ex.20sec)a
mild footshock is delivered. After a few such associations, the sensory
stimulus alone induces a typical fear response characterized by freezing
behavior. Importantly in this paradigm, the duration of the interval
between the stimulus and the footshock is fixed and it has been shown
that time is learned extremely rapidly, from even single experiences.
The extraction of interval duration is therefore a fundamental property
of learning (Gallistel and Balsam, 2014) and allows the organism to
anticipatetheproductionofanadaptedresponsetoafrighteningevent.
The aim of the current project is to better understand the neural
network supporting time encoding in fear conditioning in rats.
Recent preliminary data from the team suggest that time encoding
involves interactions between the amygdala and the dorsal striatum. In
order to further investigate this hypothesis, we are currently seeking at
inactivating the projection pathway between the amygdala and the
striatum using chemogenetic tools. For this we will use the combined
injection of a retrograde virus in the striatum and a virus containing a
Dreadd (Whissell et al, 2016) in the amygdala, thus allowing to
specificallyinactivatetheamygdalatostriatumprojectionpathway.
Thetraineewillparticipateinthedifferentpartsoftheexperiment:
• Adjustment of the best injection parameters (stereotaxic
surgery)
• Histological control of virus integration in the injected structures
(histology,neuroanatomy)
• Assessment of the effect of inactivation of the targeted pathway
ontimeencodinginfearconditioning(behavioranalysis)
Bibliography
Selectedpublicationsfromtheteamonthetopic
1. Boulanger Bertolus J, Mouly AM, Sullivan RM (2016) Ecologically
Relevant Neurobehavioral Assessment of the Development of Threat
Learning.Learning&Memory2016Sep15;23(10):556-66.
2. Hegoburu C, Parrot S, Ferreira G, Mouly AM (2014). Differential
involvement of amygdala and cortical NMDA receptors activation
upon encoding in odor fear memory. Learning & Memory,
21(12):651-655.
3. ShionoyaK,HegoburuC,BrownBL,SullivanRM,DoyèreV,MoulyAM
(2013). It's time to fear! Interval timing in odor fear conditioning in
rats.FrontiersinBehavioralNeuroscience,2013Sep27;7:128.
4. Hegoburu C, Shionoya K, Garcia S, Messaoudi B, Thevenet M, Mouly
AM (2011). The RUB cage: Respiration-Ultrasonic vocalizationsBehavior acquisition set-up for assessing emotional memory in rats.
FrontiersinBehavioralNeuroscience,2011May19;5:25.
5. Hégoburu C, Sevelinges Y, Thévenet M, Gervais R, Parrot S, Mouly AM
(2009). Differential dynamics of amino acid release in amygdala and
olfactory cortex during odor fear acquisition as revealed with
simultaneous high temporal resolution microdialysis. Learning &
Memory,2009,16:687-697.
Otherreferences
1. Gallistel CR, Balsam PD. Time to rethink the neural mechanisms of
learningandmemory.NeurobiolLearnMem.2014Feb;108:136-44.
2. Whissell PD, Tohyama S, Martin LJ. The Use of DREADDs to
DeconstructBehavior.FrontGenet.2016May17;7:70. Location
Duration
Language
(French/English/Bo
th)
CentredeRechercheenNeurosciencesdeLyon,
CNRSUMR5292-INSERMU1028-UniversitéLyon1
50AvenueTonyGarnier
F-69366LYONCedex07
France
3-4months
Both