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Middle School Curriculum Unit 17: Biotechnology Lesson 4: Genetic Engineering STUDENT OBJECTIVES 1. Explain genetic engineering 2. Describe a procedure of genetic engineering 3. Identify the process of DNA Fingerprinting 4. Complete the lab(s) 5. Explain how genetic engineering is applied in agriculture 6. Cite examples of genetic engineering uses in agriculture Agriculture QCC:…………………………………………………………………..20, 24 Language Arts QCC:……………………………………………...……………..6-16, 44 7-20, 40 8-20, 41 Science QCC:……………………………………………...…………………….….… 6-1 7-1, 10, 11 8-1 REFERENCES National Council for Agricultural Education. Biotechnology for Plants, Animals and The Environment. “Genetic Engineering” Texas A&M University. http://services.juniata.edu/ScienceInMotion/bio/labs/Biotech/DNA Profiling.doc The Biotechnology Education Project. An Introduction to Biotechnology. St Louis, Missouri. Lee, Jasper S. and Diana L. Turner. Introduction to World: Agriscience and Technology. Danville, IL: Interstate Publishers, Inc. http://nesen.unl.edu/methods/biodemo.html#demo1 EQUIPMENT, SUPPLIES, MATERIALS Construction paper, glue, staples, scissors, and worksheet for lab(s) PowerPoint presentation TERMS TO FAMILIARIZE YOUR STUDENTS WITH DNA Fingerprinting Restriction sites Restriction enzymes Teaching Procedure A. Introduction DNA fingerprinting is mostly used in crime cases where the courts need to find out who did the crime. Fingerprinting involves running a segment of DNA in an agarose gel and looking at the results to see who did it. The victim’s DNA and the Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 suspects’ DNA are all included on the agarose gel and based on the pattern seen, one can tell who did the crime and now will do the time. More than one hundred acres of genetically engineered crops are in the world today. Some of these crops include soybeans, corn, cotton, and canola. B. Motivation 1. Understand the concept of DNA fingerprinting 2. Do the lab for “Paper Gene Splicing” 3. Submit accurate results for the lab 4. Completion of the “Horse and Foal” worksheet C. Discussion 1. Question: What is genetic engineering? Answer: Also called recombinant DNA technology or gene manipulation. Two major techniques in genetic engineering; 1) genes are engineered into organisms such as microbes (bacteria) for mass-production and 2) insertion of select genes into organisms to improve their genotype. For plants and animals we use tissue culturing to increase the number in bulk quantity. 2. Identify the procedure of genetic engineering. Answer: 1) Isolating genes: Put the gene on a map, scientists know the restriction sites around it and cut it from DNA. This is useful to isolate short genes. 2) Recombination: Gene is linked to larger section of modified bacterial DNA. 3) Transformation: Combined plasmid/gene DNA inserted into target cell. When bacteria and plasmid are mixed they are given a shock to make them permeable. Transformation adds genes to the whole organism. 4) Screening: Used to check if gene of interest is present. 5) Induction: In front of a new gene genetic engineers attach a promoter, extra set of base pairs that control the activation of the gene. 3. Question: Describe the gel electrophoresis process. Answer: It uses an electric current to separate various samples of DNA to different lengths in the gel. DNA fragments are attracted to the positive side of the pole. The larger the fragments the slower it migrates. 4. Question: What field of science is DNA fingerprinting closely associated with? Answer: Genetics 5. Question: How is genetic engineering applied in agriculture? Answer: Crops have been developed that resist insect damage and spoilage. Some species of plants have the ability to kill off weeds around them. This would cut down on the level of herbicides sprayed. Scientists inserted genes that prolong the shelf life of fruit and vegetables. Think back to the article on corn from Lesson 2 how was genetic engineering involved there? Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 6. Question: What are some uses of genetic engineering in agriculture? Answer: Black walnut has the ability to kill off certain species that grow near it. Once this gene is isolated, genetic engineers can move it into other plants to kill off weeds and roots. The tomato, the first genetically modified food, has been experimented on with its shelf life with delayed ripening. The corn-borer-resistant corn has a pest-resistant trait. Cotton is herbicide resistant. Having miniature-sized watermelons improves the eating quality. Improved sweetness gives sweet peas a better taste. Strawberries can withstand freezing temperatures. Potatoes absorb less oil when fried thanks to their high starch content. Bananas may provide immunity to hepatitis thanks to a Hepatitis B virus protein added to the fruit. Squash has been genetically engineered to resist two viruses. Crops can also be genetically engineered to be resistant to frost damage. 7. Question: How do genetically engineered crops help society? Answer: Scientists are now modifying crops to produce pharmaceutical products. Some genetically engineered plants can help in producing human antibodies for anti-cancer and anti-virus drugs. Some consumers are still hesitant to eat genetically engineered food because they are fearful of the compounds and chemicals used to make it. If you had a choice between a regular tomato and a genetically modified tomato, which would you choose? Does it really matter to you? D. Lab Activities Gene Splicing Materials: Construction paper, glue, scissors, stapler with staples Procedure: Before class, cut a long thin strip of construction paper and glue or staple it into a loop. When discussing gene splicing, cut the loop (plasmid) with the scissors (restriction enzyme). Then with glue or stapler (ligase), attach another strip of construction paper (inserted gene) into the loop. Optional Lab Activity Horse and Foal Lab A horse breeder has reason to suspect that his neighbor stole one of his purebred foals. His neighbor is willing to return the foal only if the breeder can prove that the foal is his! How do you do it? See lab worksheet in Disk 1, Unit 17 Biotechnology under the folder entitled Supplemental Worksheets. Optional Lab Activity The Beachy Experiment (aka Virus Cutting) Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 This experiment performed by Roger Beachy explains how plants can become virus resistant. Beachy introduced a virus gene called TMV, tobacco mosaic virus. He isolated the virus and inserted it into the Ti plasmid of a bacterium. Then the TMV gene transfers into a plant cell providing “immunity” to the virus. The cells are grown into a culture and then become a plant resistant to TMV. Students will be simulating this experiment with the help of plasmid worksheets, scissors, gluestick, and Ziploc bags. Materials: Paper with four circular plasmids on it (See following handout; also found in Disk 1, Unit 17, Supplemental Worksheets, and Beachy Experiment) Scissors Gluestick Plastic Ziploc bag(s) Storage Size Ziploc bag Yarn Procedure: 1. The students must build their own Tobacco mosaic virus out of three of the plasmids on the worksheet. 2. Cut out three of the four plasmids. Cut at the points marked B, C, D, and E. Paste B to C and C to D and then D to E. This should give you a spiral. 3. After the students have made their virus, the teacher will write on the board the segment of the virus the students should cut out. 4. After cutting that certain segment, find the appropriate place on the Ti plasmid. (Use the base pairing method where A pairs with T and G pairs with C) 5. Place the new plasmid inside a Ziploc bag with the yarn to simulate the new bacterium. 6. The new bacterium will then be placed into a plant cell providing “immunity” to the tobacco mosaic virus. 7. When the new plant cells are grown in a culture, we develop a new plant that is resistant to TMV (tobacco mosaic virus). E. Optional Lab Activities 1. DNA Murder Mystery Lab (Carolina Math and Science Catalog) ER #17-1028. Matching “pop bead” fingerprints of suspects to find out who did the crime. Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 ACADEMIC CONNECTIONS Science Middle School Connections in Agriculture Education Science QCC: Grade 6- 1 7- 1, 10, 11 8- 1 Name Period: Date: What traits are visible in a human population? Purpose: To identify certain traits in a human population. Procedure: On a separate sheet of paper list the names of all the members in your family. Ask them the following questions. Be sure to record their response as either “yes” or “no”. 1. 2. 3. 4. 5. Can you roll your tongue? Do you have naturally straight hair? Do you have brown eyes? Do you have attached earlobes? Do you have good vision, without eyeglasses? After you have asked the questions record their responses in the following table. Family Member Roll your tongue? Naturally straight hair? Inherited Trait Brown eyes? Attached Good vision ear lobes? without glasses? 1. 2. 3. 4. 5. 6. 7. Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 ACADEMIC CONNECTIONS Language Arts Middle School Connections in Agriculture Education Language Arts QCC: Grade 6-16, 44 7-20, 40 8-20, 41 Name____________________________________ Period____ Date______________ Teacher’s Note: Have students complete a lab activity, related to the material discussed in class. After completing your lab, respond to the following questions. 1. What was the title of the lab? 2. What was the purpose or objective of the lab? 3. Write a short summary of the steps in the lab. 4. What were your results? 5. What do these results mean? Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 Summary Conclusion DNA fingerprinting is a real-world example of how genetics and technology come together to benefit society. DNA fingerprinting solves the “who did it?” cases in courts today. Many cases still go unsolved due to the fact that there is not enough evidence to convict the criminal. Evaluation Written quiz Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 A C B D E Don’t Cut this circle!! Cut at points labeled B, C, D, and E. Paste B to C and D to E. That is your virus! Look for the sequence your teacher has chosen to cut. Then match that with the plasmid, the circle you did not cut. Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004 Middle School Curriculum Unit 17: Biotechnology Lesson 4: Genetic Engineering Worksheet A horse breeder had his purebred foal stolen from the barn late one night. Four years later, his neighbor bought a horse and the breeder had reason to believe that his neighbor’s new horse was actually the foal stolen from his barn. His neighbor will be happy to return the horse if the neighbor can prove the horse is his! How can this be done? DNA Strands from the Horses Foal born in 1999 Horse Bought in 2003 On a separate sheet of paper, write your answers to the following questions: Are there any similarities between the two? What leads you to believe that this horse might be the breeder’s foal? Prove your answer with evidence from the DNA strands. Georgia Middle School Agricultural Education Curriculum, Unit 17 Lesson 4 Updated May 2004