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Hugin and its receptor signaling modulates post-mating food preference
decision in Drosophila melanogaster
Sung-Eun Yoon, Na-Rae Lee, Young-Joon Kim
School of Life Sciences, Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Bukgu, Gwangju 500-712, Republic of Korea
In animals, the feeding behavior is genetically programmed, yet modulated according to their
nutritional and physiological states. Fruit fly Drosophila melanogaster makes mating statusspecific food preference decisions. Given with a choice between yeast and sucrose, male and
virgin female prefer sucrose to yeast. After mating, female undergoes a switch, and prefers yeast
to sucrose. A vertebrate neuropeptide, neuromedin U (NMU) plays an important role in
modulating central energy homeostasis including feeding behavior. Here, we report that hugin,
Drosophila counterpart of NMU and its receptor signaling modulate post-mating food preference
decisions in female. To investigate molecular and neural mechanisms underlying the post-mating
food preference switch, we manipulated activities of neurons producing hugin peptides or its two
receptors, CG8784 and CG8795. Virgin females carrying hugin cell-knockout (hugin-KO) showed
the strong preference toward yeast, reminiscent of that of the mated. This phenotype is also
observed in virgins carrying CG8784-KO or CG8795-KO. Moreover, females from CG8795 null loss
of function mutants also showed the same phenotype as hugin-KO and CG8795-KO do. The post-
mating behavioral changes in females are triggered by sex peptide (SP), a seminal protein
transferred from male during copulation. To determine whether hugin or its receptor neurons
are targets of SP, we activated SP signaling in those neurons by expressing membranetethered SP (mSP). The activation of SP receptor (SPR) signaling in neurons expressing hugin,
CG8795 but not CG8784 makes the virgin prefer yeast to sucrose. However, SPR-knockdown
in those neurons did not alter yeast preference of the mated.