Download I have.. Who has.. DNA produced from mRNA by reverse

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DNA produced from
mRNA by reverse
transcription
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cDNA
PCR
Ligase
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Enzyme that joins
DNA fragments
“False” nucleotide
used in DNA
sequencing nucleotide analogue.
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Method of
amplifying (cloning)
DNA in vitro.
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dideoxynucleotide
Plasmid
Gene cloning
Reverse transcriptase
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Miniature, circular
Method of
bacterial chromosome amplifying (cloning)
used in cloning DNA
DNA in vivo.
by bacteria.
Enzyme used to
make DNA from
mRNA
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Restriction enzyme
Gel electrophoresis
Annealing
Bacteriophage
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Technique used for
separating DNA
fragments of different
lengths, using an
electric field in a
porous gel.
Forming a
temporary join
between DNA
fragments by
complementary
base pairing
Virus that attacks
bacteria and is used
in cloning DNA
Capable of
developing into the
full range of cell
types in an organism.
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Totipotent
Gene probe
Recognition site
Primer
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Short length of single
stranded DNA used to
locate a specific DNA
sequence
Specific sequence of Short length of DNA
bases recognised by used to begin DNA
a restriction
replication
enzyme –
palindromic.
Enzyme that cuts
DNA at specific
sequences.
Part of a gene that is
not expressed as a
polypeptide.
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Intron
Retrovirus
Taq polymerase
Exon
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Virus that uses reverse
transcription to make
complementary DNA
in the host cell.
Enzyme extracted
from a heat-loving
bacteria that has an
optimum
temperature of
80ºC. Used in PCR.
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Part of a gene that is
expressed as an
amino acid sequence
of a protein
Sections of noncoding DNA
consisting of
repeated sequences
two to six bases long
- microsatellite.
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STR
VNTR
Transgenesis
Agarose gel
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Sections of noncoding DNA consisting
of repeated
sequences, the
number of repeats
varying from one
individual to another minisatellite.
The artificial
transfer of DNA
from one species to
another
Electrophoresis gel
with larger pores
suitable for sorting
longer DNA
fragments and those
that vary widely in
length
Electrophoresis gel
with smaller pores
suitable for sorting
shorter fragments
including those
differing in length by
a single nucleotide.
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Polyacrylamide gel
DNA Profiling.
Kanamycin
Autoradiograph
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Antibiotic used to
detect whether the
antibiotic resistance
gene has been
taken up by
bacterial cells
Image on a
photographic plate
showing distribution
of a radioactively
labelled substance.
Membrane on which
separated DNA
fragments are
deposited
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Nitrocellulose
Southern Blotting
kB
Stem cells
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Process that identifies
the natural variations
found in the DNA of
humans and other
organisms and
presents them for use.
Technique for
A sequence of 1000
transferring DNA from bases on DNA
electrophoresis gel to
nitrocellulose or nylon
membrane.
Cells that have the
capacity to
differentiate into a
range of cell types