Download Particle Size of Beta Amyloid Peptide Aggregates Using Dynamic

Journal of Behavioral and Neuroscience Research
2007, Vol. 5 (NEURON Special Issue), 18
© 2007 The College of Saint Rose
POSTER ABSTRACT
Particle Size of Beta Amyloid Peptide Aggregates Using
Dynamic Light Scattering
K. Centrella, E. Hart, B. Gray, & J. Roecklein-Canfield
Simmons College
It has been previously demonstrated that exposure of aged aggregates of beta
amyloid peptide 1-42 to embryonic chick ciliary ganglion (CG) neurons inhibits
potassium-evoked ACh release. The A? aggregates (at a concentration of 10 um)
have to be preincubated (aging) for at least 72 hours at 37 C after solubilizing
lyophilized peptide monomers in water. This requirement may be due to time
required for A? peptides to aggregate into protofibrils. Dynamic Light Scattering
(DLS) was used to examine structural changes during the aging process in order
to correlate structure with the onset of inhibitory properties. In this summer’s
experiments, A? aggregates displaying large radii as measured by DLS were able
to inhibit labeled acetylcholine release from primary cultures of CG neurons.,
whereas smaller radii aggregates were not. Supported by Merck AAAS grant
award to Simmons College.
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