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Download Particle Size of Beta Amyloid Peptide Aggregates Using Dynamic
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Journal of Behavioral and Neuroscience Research 2007, Vol. 5 (NEURON Special Issue), 18 © 2007 The College of Saint Rose POSTER ABSTRACT Particle Size of Beta Amyloid Peptide Aggregates Using Dynamic Light Scattering K. Centrella, E. Hart, B. Gray, & J. Roecklein-Canfield Simmons College It has been previously demonstrated that exposure of aged aggregates of beta amyloid peptide 1-42 to embryonic chick ciliary ganglion (CG) neurons inhibits potassium-evoked ACh release. The A? aggregates (at a concentration of 10 um) have to be preincubated (aging) for at least 72 hours at 37 C after solubilizing lyophilized peptide monomers in water. This requirement may be due to time required for A? peptides to aggregate into protofibrils. Dynamic Light Scattering (DLS) was used to examine structural changes during the aging process in order to correlate structure with the onset of inhibitory properties. In this summer’s experiments, A? aggregates displaying large radii as measured by DLS were able to inhibit labeled acetylcholine release from primary cultures of CG neurons., whereas smaller radii aggregates were not. Supported by Merck AAAS grant award to Simmons College. 18