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Download CHARGE Region Probe - FISH Probes from Cytocell
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Patents and Trademarks Aquarius and Cytocell are registered trademarks of Cytocell Ltd. This product contains technology licensed from Life Technologies Corporation and is available for human diagnostics or life science research use only. Cytocell Ltd. 3-4 Technopark Newmarket Road Cambridge, CB5 8PB, UK. T: +44(0)1223 294048 F: +44(0)1223 294986 E: [email protected] W: www.cytocell.com CHARGE Region Probe REF: LPU 021-SA / LPU 021-A Analyte Specific Reagent: Analytical and performance characteristics are not established. Fluorescence In Situ Hybridisation (FISH) is a technique that allows DNA sequences to be detected on metaphase chromosomes or in interphase nuclei from fixed cytogenetic samples. The technique uses DNA probes that hybridise to entire chromosomes or single unique sequences, and serves as a powerful adjunct to classic cytogenetics. Recent developments have meant that this valuable technique can now be applied as an essential tool in prenatal, haematological and pathological chromosomal analysis. Target DNA, after fixation and denaturation, is available for annealing to a similarly denatured, fluorescently labelled DNA probe, which has a complementary sequence. Following hybridisation, unbound and non-specifically bound DNA probe is removed and the DNA is counterstained for visualisation. Fluorescence microscopy then allows the visualisation of the hybridised probe on the target material. Probe Specification CHD7, 8q12.1-q12.2, Red D8Z2, 8p11.1-q11.1, Green D8Z2 8q12.1-q12.2 CHD7 RAB2A D8S1929 100kb D8S2048 D8S1986 D8S260 310kb The CHD7 probe is 310kb, labelled in red and covers a region including the entire CHD7 gene as well as the D8S1929 and D8S260 markers. The probe mix also contains a control probe for the chromosome 8 centromere (D8Z2), labelled in green. Materials Provided Probe: 50µl per vial or 100µl per vial The probe is provided in hybridisation solution (Formamide; Dextran Sulphate; SSC) and is ready to use. Warnings and Precautions 1. For professional use only. 2. Wear gloves when handling DNA probes. 3. Probe contains formamide, which is a teratogen; do not breathe fumes or allow skin contact. Wear gloves, a lab coat, and handle in a fume hood. Upon disposal, flush with a large volume of water. 4. All hazardous materials should be disposed of according to your institution’s guidelines for hazardous waste disposal. Storage and Handling The probe should be stored at -20ºC until the expiry date indicated on the label. The probe vial must be stored in the dark. Additional Information For additional product information please contact the Cytocell Technical Support Department. T: +44 (0)1223 294048 E: [email protected] W: www.cytocell.com DS109 v005.00/2014-06-27 Page 1 of 1