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Chapter 7: Cell Structure and Function BIOLOGY I SEMESTER TWO Robert Hooke Mid-1600s: First to describe cells Actually looking at dead cork cells Hooke’s Compound Microscope Anton van Leeuwenhoek Describes several types of cells First to study living, moving organisms (from pond water) with microscope P. 182-183 Cell Theory The cell theory is a fundamental concept of biology! It states: 1. All living things are composed of cells. 2. Cells are the basic units of structure and function in living things. 3. New cells are produced from existing cells. Compound Microscope Staph bacteria under a compound microscope Electron Microscope Transmission (TEM) Specimen cut into very thin slices Beam of electrons pass through Scanning (SEM) Samples are dehydrated, put in a vacuum, and sometimes coated in materials like gold Electrons are bounced off the surface Produces 3-D images of the surface Electron Microscope Staph bacteria under an electron microscope Other EM Images Clockwise from top left: spider, bacteria, microorganism (probably bacteria) Classifying Cells – RA Activity Read the handout about Prokaryotic and Eukaryotic Cells and talk to the text. 2. Construct a Double Bubble Map comparing and contrasting prokaryotic and eukaryotic cells. 3. Be prepared to share with your partner, and then with the class! 1. Classifying Cells Prokaryotes Lack organized structures (organelles) Circular loop of DNA No nucleus Examples: bacteria and blue-green algae Eukaryotes Organized structures called organelles DNA in nucleus Examples: animal, plant, fungi, and protists Eukaryotic Cells Three regions 1. 2. 3. Cell membrane Nucleus Cytoplasm Cell (Plasma) Membrane Cell (Plasma) Membrane Function Regulates what comes in and out of cell Selective permeability page 72 of your CH 7 Reading Guide! Communication Protection and Support Phospholipid Bilayer Double layer of phospholipids Fluid Mosaic – read about on page 74 of CH 7 R. Guide! Phospholipid molecules with other molecules (proteins and carbohydrates) embedded in it The membrane is in constant movement Phospholipids - Revisited Polar Head Hydrophilic Attracted to water Non-polar Tail Hydrophobic Doesn’t want to be near the water Membrane Proteins Allow larger molecules to pass through the membrane Can regulate/control what comes in or out (is the cell’s bodyguard!) Other Molecules Cholesterol Stabilizes the membrane Keeps non-polar tails from sticking to each other Carbohydrate chains Identification Markers: communicates type of cell Cell Wall Plants only Rigid outer layer covering the cell membrane Allow plants to support heavy structures like flowers Contains cellulose and various proteins Movement Across Membranes Passive Transport Diffusion (Simple and Facilitated) Osmosis Active Transport Protein Pump Endocytosis and Exocytosis Movement is controlled by concentrations Concentration Amount of solute (dissolved substance) in a volume of solution Expressed as mass/volume Amount of mass is proportional to the concentration Volume is inversely proportional to the concentration Passive Transport Movement from an area of high concentration to an area of low concentration (down the concentration gradient) Requires NO energy Examples: Diffusion (simple and facilitated) and Osmosis Diffusion (Simple) Movement of solute from high to low concentrations Requires no energy Continues until dynamic equilibrium is reached Facilitated Diffusion Solute cannot simply cross cell membrane because it is semi-permeable Solute diffuses through membrane proteins Allows diffusion of molecules that are too large to diffuse through the membrane using simple diffusion Osmosis Movement of water from an area of high concentration to low concentration of water Requires no energy Why do cells do Osmosis? Solutions surrounding cells can be…(read on page 81-82 in Reading guide!) Hypertonic – solution has higher solute concentration compared to the inside of the cell Isotonic – solution has the same solute concentration as the inside of the cell Hypotonic – solution has a lower solute concentration compared to the inside of the cell Active Transport Movement of solute from an area of low concentration to high concentration (against or up the concentration gradient) Requires energy (using ATP) Examples: Protein pumps, endocytosis, and exocytosis Protein Pumps Membrane protein pumps solute across cell membrane Solute moving from low to high concentration Requires ATP energy Endocytosis and Exocytosis BOTH: Movement of large materials across the cell membrane Read and take your own notes about each type – page 82 of Reading Guide Endocytosis Movement into cell Pocket of membrane pinches off to form vesicle (membrane circle surrounding material) Exocytosis Movement out of cell Endocytosis Exocytosis Cytoplasm The cytoplasm includes everything INSIDE the cell membrane except the nucleus Also includes the fluid cytosol Where do Organelles Come From? Thought to originally be prokaryotes that formed a symbiotic relationship with What is symbiosis?! another cell Called the Endosymbiotic Theory Evidence Many organelles are surrounded by two membranes Some organelles contain their own DNA Nucleus Control center of the cell Double membrane with many pores Contains DNA Directions for proteins Chromatin Chromosomes Nucleolus Small, dark region Makes ribosomes Ribosomes Site of protein synthesis Link amino acids together to form proteins Two subunits made of RNA and protein Found free floating in cytoplasm or attached to rough ER Endoplasmic Reticulum Series of membrane bound canals Two Types 1. Rough ER Studded w/ ribosomes Produces and transports Proteins 2. Smooth ER No ribosomes Produces and transports Lipids and Carbs Detoxifies your blood in liver cells Golgi Apparatus Stack of flattened pancake-like membranes Modifies, packages, and ships out lipids and proteins Lysosome Vesicle filled w/ digestive enzymes Breaks down cellular waste, bacteria and viruses Aids in programmed cell death (apoptosis) Vacuole Animals Many small membrane bound sacs OR none Storage compartments for water, food molecules, ions, and enzymes Plants Usually one large central vacuole Used for storage of water, toxins, waste products, and pigments that give color (like in flower petals) Water storage helps establish turgor pressure to keep plant upright Mitochondria Double membrane Inner membrane highly folded Powerhouse of cell (makes ATP’s) Only inherited from mother Contains DNA Chloroplast Only in Plants Double membrane Converts sunlight energy into chemical energy Contains chlorophyll pigment Captures light energy Contains DNA Cytoskeleton Skeleton for Cell Helps cell maintain shape Provides support and protection Aids in movement Made of microtubules and microfilaments Cilia Short, hair-like microtubule extensions Move in oar-like motion Move cell or move materials on the surface of cells Cells usually have many Flagella Long, whip-like microtubule extension Move in whip-like fashion Moves cells Cells usually only have one or a few Centrioles Only in animal cells Grouped microtubules Can form cillia or flagella Aids in cell division Moves chromosomes with spindle fibers Animal Vs. Plant Cells Organelle Animal Plant Nucleus YES YES Cytoplasm YES YES Cell membrane YES YES Cell wall NO YES Lysosome YES YES Ribosome YES YES ER YES YES Mitochondria YES YES Animal Vs. Plant Cell Continued Organelle Animal Plant Golgi YES YES DNA YES YES Vacuole YES (small, several, only in a few animal cells) YES (large, single) Cytoskeleton YES YES Chloroplast NO YES Centriole YES NO Flagella YES (some) NO Cilia YES (some) NO Cell Thinking Map! Make a brace map of all the parts of the cell and how they fit together. Skill: Whole to Part Relationship Unicellular Organisms Organism made of a single cell Very simple One cell performs all the functions of life Ex: Bacteria, protists, some fungi Multicellular Organisms Organisms made of many cells More complex Cells specialize and perform certain functions (cell differentiation) All cells work together to perform all the functions of life Ex: animals, plants, and fungi Levels of Organization Levels of Organization Atom Molecule Macromolecule Cell Tissue- specialized cells working together towards a common goal Organ- tissue working together towards a common goal Organ system- organ working together towards a common goal Organism