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Interactions of TCA cycle enzymes and of the CcpA-HPrSer46P complex with various cre-elements in Bacillus subtilis Interaktionen von Enzymen des Citratzyklus und des CcpA-HPrSer46P-Komplexes mit verschiedenen cre-Elementen in Bacillus subtilis Der Naturwissenschaftlichen Fakultät der Friedrich-Alexander-Universität Erlangen-Nürnberg zur Erlangung des Doktorgrades Dr. rer. nat. vorgelegt von Maike Bartholomae aus Wassertrüdingen Als Dissertation genehmigt von der Naturwissenschaftlichen Fakultät der Friedrich-Alexander-Universität Erlangen-Nürnberg Tag der mündlichen Prüfung 02. Mai 2013 Vorsitzender der Promotionskommission: Prof. Dr. Johannes Barth Erstberichterstatter: Prof. Dr. Andreas Burkovski Zweitberichterstatter: Prof. Dr. Jörg Stülke Table of contents 1 Zusammenfassung_____________________________________________________ - 1 - 2 Summary ____________________________________________________________ - 2 - 3 Introduction __________________________________________________________ - 3 3.1 The Gram-positive model organism Bacillus subtilis____________________________ - 3 - 3.2 Metabolon formation in B. subtilis _________________________________________ - 3 - 3.2.1 TCA cycle as central part of metabolism and anabolism ________________________________ - 4 - 3.2.2 Regulation of mdh, icd and citZ ___________________________________________________ - 5 - 3.2.3 The NADP dependent isocitrate dehydrogenase Icd __________________________________ - 7 - 3.2.4 The malate dehydrogenase Mdh __________________________________________________ - 8 - 3.2.5 The citrate synthase CitZ _________________________________________________________ - 9 - 3.3 Regulation of carbon metabolism of B. subtilis _______________________________ - 11 - 3.3.1 The phosphoenolpyruvate: sugar phosphotransferase system (PEP:PTS) _________________ - 11 - 3.3.2 The catabolite control protein A and CCR in B. subtilis ________________________________ - 12 - 3.3.3 The catabolite responsive element cre ____________________________________________ - 15 - 3.3.4 The regulation of ackA, gntR, xylA and xynP ________________________________________ - 16 - 3.4 4 + Scope of the thesis _____________________________________________________ - 17 - Material and Methods ________________________________________________ - 18 4.1 Material ______________________________________________________________ - 18 - 4.1.1 Chemicals ____________________________________________________________________ - 18 - 4.1.2 Auxiliary Material _____________________________________________________________ - 20 - 4.1.3 Instruments __________________________________________________________________ - 21 - 4.1.4 Reagent mixtures _____________________________________________________________ - 22 - 4.1.5 Proteins, enzymes and standards _________________________________________________ - 22 - 4.1.6 Oligonucleotides ______________________________________________________________ - 23 - 4.2 Bacterial strains _______________________________________________________ - 24 - 4.3 Plasmids _____________________________________________________________ - 24 - 4.4 Buffer and media ______________________________________________________ - 25 - 4.4.1 General buffers and solutions ____________________________________________________ - 25 - 4.4.2 Buffers and solutions for protein purification _______________________________________ - 25 - 4.4.3 DNA and protein gel electrophoresis ______________________________________________ - 26 - 4.4.3.1 DNA gel electrophoresis ____________________________________________________ - 26 - 4.4.3.2 Protein gel electrophoresis _________________________________________________ - 26 - 4.4.4 Media and supplements ________________________________________________________ - 28 - I Table of contents 4.5 Methods _____________________________________________________________ - 29 - 4.5.1 General methods ______________________________________________________________ - 29 - 4.5.2 Growth of bacteria ____________________________________________________________ - 29 - 4.5.3 Transformation of E. coli ________________________________________________________ - 29 - 4.5.3.1 Preparation of CaCl2 competent E. coli cells ____________________________________ - 29 - 4.5.3.2 Transformation of E. coli ___________________________________________________ - 30 - 4.5.3.3 Long time storage of bacterial strains _________________________________________ - 30 - 4.5.4 Methods for purification and modification of nucleic acids ____________________________ - 30 - 4.5.4.1 Hybridization of cre DNA ___________________________________________________ - 30 - 4.5.4.2 Isolation of chromosomal DNA from B. subtilis _________________________________ - 31 - 4.5.4.3 Isolation of plasmid DNA from E. coli _________________________________________ - 31 - 4.5.4.4 Polymerase chain reaction (PCR) _____________________________________________ - 32 - 4.5.4.5 Restriction of DNA ________________________________________________________ - 32 - 4.5.4.6 Dephosphorylation of DNA _________________________________________________ - 33 - 4.5.4.7 Ligation of DNA fragments __________________________________________________ - 33 - 4.5.4.8 Elution of DNA from agarose gels ____________________________________________ - 33 - 4.5.4.9 Sequencing of DNA ________________________________________________________ - 33 - 4.5.5 Methods for purification and analysis of proteins ____________________________________ - 34 - 4.5.5.1 Denaturating protein gel electrophoresis (PAGE) ________________________________ - 34 - 4.5.5.2 Native PAGE _____________________________________________________________ - 34 - 4.5.5.3 Cell cultivation for protein overexpression _____________________________________ - 34 - 4.5.5.4 Sonication _______________________________________________________________ - 34 - 4.5.5.5 Overexpression test _______________________________________________________ - 35 - 4.5.5.6 Purification of CcpA(His)6, Mdh(His)6, Icd(His)6, IcdS104P(His)6 and CitZ(His)6 from Bacillus subtilis _______________________________________________________________________ - 35 - 4.5.5.7 Preparation of E. coli DH5α p4813/ HPr kinase extract ___________________________ - 36 - 4.5.5.8 Purification of HPrSer46P ___________________________________________________ - 36 - 4.5.5.9 Dialysis of protein solutions _________________________________________________ - 37 - 4.5.5.10 Surface plasmon resonance (SPR) analysis _____________________________________ - 37 - 4.5.5.11 Icd activity assay __________________________________________________________ - 39 - 4.5.5.12 Mdh activity assay ________________________________________________________ - 40 - 4.5.5.13 CitZ activity assay _________________________________________________________ - 40 - 4.5.6 Computer programs, databases and online services __________________________________ - 41 - 4.5.6.1 Computer programs _______________________________________________________ - 41 - 4.5.6.2 Databases and online services _______________________________________________ - 41 - II Table of contents 5 Results _____________________________________________________________ - 43 5.1 Metabolite dependent interaction of Isocitrate dehydrogenase with Malate dehydrogenase of Bacillus subtilis _______________________________________________ - 43 5.1.1 Purification of Mdh(His)6, Icd(His)6 and IcdS104P(His)6 ________________________________ - 43 - 5.1.2 Interaction of Mdh(His)6 and Icd(His)6 _____________________________________________ - 44 - 5.1.3 SPR analysis of the interaction of Icd(His)6 and Mdh(His)6 in the presence of cofactors and substrates __________________________________________________________________________ - 45 5.1.3.1 SPR analysis of Icd(His)6 binding to Mdh(His)6 in the presence of isocitrate, α-ketoglutarate, malate and oxaloacetate ____________________________________________________________ - 45 5.1.3.2 5.2 Influence of the enzymatic activity of Icd(His)6 on the binding to Mdh(His)6 __________ - 47 - Interaction of Mdh(His)6 and IcdS104P(His)6 _________________________________ - 48 - 5.2.1 Activity of Icd(His)6 and IcdS104P(His)6 ____________________________________________ - 48 - 5.2.2 SPR analysis of IcdS104P(His)6 binding to Mdh(His)6 __________________________________ - 49 - 5.2.3 SPR analysis of IcdS104P(His)6 binding to Mdh(His)6 in the presence of isocitrate, α-ketoglutarate, malate and oxaloacetate ______________________________________________________________ - 50 5.2.4 Quantification of the interaction between IcdS104P(His) 6 and Mdh(His)6 stimulated by isocitrate, + NADP and MgCl2 ____________________________________________________________________ - 52 - 5.3 Mutual influence of Icd and Mdh on their enzymatic activity ___________________ - 53 - 5.3.1 Influence of Icd and IcdS104P on Mdh activity ______________________________________ - 54 - 5.3.2 Influence of Mdh on the enzymatic activity of Icd and IcdS104P ________________________ - 56 - 5.4 Interaction analysis of Malate dehydrogenase and Citrate synthase _____________ - 58 - 5.4.1 Purification of CitZ(His)6 ________________________________________________________ - 58 - 5.4.2 SPR analysis of CitZ(His)6 binding to Mdh(His)6 ______________________________________ - 59 - 5.4.3 SPR-analysis of CitZ(His)6 and Mdh(His)6 interaction in the presence of malate and oxaloacetate ____________________________________________________________________________ - 59 - 5.5 Interaction of various cre-elements with the CcpA-HPrSer46P complex ___________ - 61 - 5.5.1 Purification of CcpA(His)6 and HPrSer46P __________________________________________ - 61 - 5.5.1.1 Purification of CcpA(His)6 ___________________________________________________ - 61 - 5.5.1.2 Purification of HPrSer46P ___________________________________________________ - 62 - 5.5.2 Qualitative and quantitative analysis of CcpA coeffector complexes interacting with various cre- elements ___________________________________________________________________________ - 63 5.5.2.1 Selection and preparation of the analyzed cre-elements __________________________ - 63 - 5.5.2.2 Qualitative analysis of CcpA-HPrSer46P binding to xylA-cre _______________________ - 64 - 5.5.2.3 Quantification of the CcpA HPrSer46P xylA-cre interaction ________________________ - 65 - 5.5.2.4 Quantification of CcpA HPrSer46P complexes interacting with xynP-cre, ackA-cre2, gntR- credown and syn-cre _________________________________________________________________ - 66 - III Table of contents 6 Discussion __________________________________________________________ - 69 6.1 Indications for the formation of a TCA cycle metabolon _______________________ - 69 - 6.2 Structure and kinetics of the CcpA-HPrSer46P complex interacting with various cre- elements ___________________________________________________________________ - 75 6.3 Outlook ______________________________________________________________ - 79 - 7 Bibliography ________________________________________________________ - 80 - 8 Abbreviation index __________________________________________________ - 102 - IV Zusammenfassung 1 Zusammenfassung Metabolons sind supramolekulare Komplexe aus Enzymen, welche aufeinanderfolgende Reaktionen katalysieren. Da Metabolons den Transfer von Metaboliten zwischen den einzelnen Enzymen erleichtern, sind möglicherweise auch die einzelnen Enzyme des Citratzyklus in Bacillus subtilis in einem Multienzymkomplex angeordnet. Kürzlich publizierte in vivo Analysen deuten darauf hin, dass die Malatdehydrogenase Mdh, die Isocitratdehydrogenase Icd und die Citratsynthase CitZ das Kernstück eines Citratzyklusmetabolons in B. subtilis bilden. Deswegen wurde in dieser Arbeit die Interaktion zwischen den oben genannten Enzymen und die Dynamik dieser Interaktionen mittels Oberflächenplasmonenresonanz (SPR) charakterisiert. Mit derselben Messmethode wurde auch der Einfluss von Substraten und Kofaktoren auf diese Interaktionen ermittelt. Diese Analysen zeigten eine schwache, aber spezifische Interaktion zwischen Mdh und Icd, welche ausschließlich durch die Substrate von Icd, die Kombination von Isocitrat, NADP+ und Mg2+, stimuliert wurde. Dieser stimulatorische Effekt ist hochspezifisch, da keine weiteren Metabolite und Kofaktoren von Icd und Mdh einen Einfluß auf die Interaktion von Mdh und Icd zeigten. Um die Dynamik der Interaktion des Icd-Isocitrat-NADP+-Mg2+-Komplexes mit Mdh zu charakterisieren, wurden kinetische SPR Analysen durchgeführt. Da Wildtyp Icd die Substrate für diese Analyse zu schnell umsetzte, wurde stattdessen eine IcdS104P Mutante eingesetzt. Die Interaktion des Enzyms mit Mdh wurde durch die Mutation nicht beeinträchtigt und wurde durch Isocitrat, NADP+ und Mg2+ stimuliert. Im Vergleich zum Wildtypenzym ist die Enzymaktivität der IcdS104P Mutante jedoch 174-fach geringer. Die Quantifizierung der Mdh-IcdS104P Interaktion ohne Substrate und Kofaktoren zeigte eine langsame Assoziationsgeschwindigkeit mit einem ka-Wert von 2,0 ± 0,2 x 102 M-1s-1 bei einer Dissoziationsgeschwindigkeitskonstante (kd) von 1.0 ± 0.07 x 10-3 s-1, woraus sich eine Dissoziationskonstante (KD) von 5,0 ± 0,1 µM ergab. In Anwesenheit von Isocitrat, NADP+ und Mg2+ nahm die Affinität von IcdS104P zu Mdh resultierend aus einer schnelleren Assoziation (ka = 1,7 ± 0,7 x 103 M-1s-1) bei langsamerer Dissoziation (kd = 2,6 ± 0,6 x 10-4 s-1) mit einem KD-Wert von 0,15 ± 0,03 µM um Faktor 33 zu. Interessanterweise wurde durch die Komplexbildung die enzymatische Aktivität von Wildtyp Icd in Gegenwart von Mdh verdoppelt. Dagegen wurde die Aktivität von Mdh durch die Anwesenheit von Icd oder IcdS104P nicht beeinflußt. Diese höhere enzymatische Aktivität von Icd in Anwesenheit von Mdh, bei gleichbleibender Mdh Aktivität führt zu einer Überproduktion an αKetoglutarat durch den Citratzyklus als Reaktion auf hohe Verfügbarkeit von Kohlenstoffquellen und NADP +, welche erhöhte anabole Anforderungen der Zelle signalisieren. Das im Überschuss synthetisierte α-Ketoglutarat kann im Aminosäureanabolismus verwendet werden, da α-Ketoglutarat als Vorläufermolekül von Glutamat dient. Eine Interaktion der beiden Enzyme Mdh und CitZ wurde auch in Anwesenheit der entsprechenden Metabolite nicht nachgewiesen. Dies stellte ein überraschendes Ergebnis dar, da die Interaktion dieser Enzyme in anderen Organismen bereits nachgewiesen wurde. Darüber hinaus wird die endergonische Umwandlung von Malat zu Oxalacetat durch Mdh nur durch die sofortige Weiterverarbeitung von Oxalacetat durch CitZ ermöglicht. Da keine direkte Interaktion zwischen Mdh und CitZ nachgewiesen wurde, ist es möglich, dass andere Faktoren, z.B. weitere Enzyme des Citratzyklus zusätzlich an deren Komplexbildung beteiligt sind. Deswegen wurde abschließend ein hypothetisches Modell des Citratzyklusmetabolons vorgestellt, welches alle Enzyme des Citratzyklus umfasst. Der zweite Teil dieser Arbeit beschäftigte sich mit der Kohlenstoffkatabolitenregulation (KKR). Dieser Mechanismus ermöglicht Lebewesen die Auswahl der Kohlenstoffquelle, welche das schnellste Wachstum ermöglicht. In B. subtilis wird die KKR durch das Katabolit Kontroll Protein A (CcpA) in Komplex mit dessen Koeffektor HPrSer46P bei Bindung an seine Operatorbindestellen („catabolite responsive elements“, cres) vermittelt. Die Transkription kataboler Gene wie z.B. gntR, xylA und xynP und von Genen des Überflußmetabolismus, z.B. ackA wird durch CcpA reguliert, jedoch mit jeweils unterschiedlichen Regulationsfaktoren. Um den Einfluß der cre-Sequenzen auf die Regulation zu ermitteln, wurden in dieser Arbeit die Kinetiken und Affinitäten der Interaktionen des CcpA-HPrSer46P Komplexes mit den cre-Sequenzen der oben genannten Gene bestimmt. Obwohl nur geringe Sequenzteile der cre-Elemente konserviert sind, ist die Affinität des CcpA-HPrSer46P Komplexes zu ackA-cre2, gntR-credown, syn-cre und xylA-cre nahezu identisch mit einem KD-Wert von 1,6-3,8 nM. Interessanterweise ist die Affinität des CcpA-HPrSer46P Komplexes zu xynP-cre mit einem KD-Wert von nur 0,2 ± 0,1 nM bis zu 19-fach erhöht. Diese höhere Affinität erklärt den hohen Repressionsfaktor von xynP und die stärkere Regulation durch CcpA im Vergleich zu gntR, xylA und ackA. -1- Summary 2 Summary Metabolons are supramolecular complexes of enzymes which catalyze subsequent reactions. Since the formation of a metabolon facilitates the transfer of metabolites, in Bacillus subtilis, the enzymes of the TCA cycle are likely organized in multienzyme complexes. Recently, in vivo analyses suggested, that the TCA cycle enzymes malate dehydrogenase Mdh, isocitrate dehydrogenase Icd and citrate synthase CitZ form the core of a TCA cycle metabolon in B. subtilis. Therefore, in this work, the interactions between these enzymes and their dynamics were characterized in vitro by surface plasmon resonance (SPR). Moreover, the impact of substrates and cofactors on this interaction was determined by SPR measurements as well. These analyses revealed a weak, but specific interaction between Mdh and Icd. Their interaction was exclusively stimulated by a mixture + 2+ of isocitrate, NADP and Mg , which are the substrates of Icd. This stimulation is highly specific, because no other metabolites and cofactors of Icd or Mdh, respectively, influenced the interaction between Mdh and Icd. + 2+ To determine the dynamics of the interaction between the Icd-isocitrate-NADP -Mg complex and Mdh, kinetic SPR analyses were performed. Since wildtype Icd converts its substrate too fast to perform kinetic studies, a IcdS104P mutant was used instead. The mutation neither influenced the interaction of the enzyme + 2+ with Mdh nor affected the stimulation of isocitrate, NADP and Mg . However, in comparison to wildtype Icd, the mutant showed a 174-fold reduced activity. The quantification of the Mdh-IcdS104P interaction revealed a 2 -1 -1 slow association rate constant (ka) of 2.0 ± 0.2 x 10 M s and a dissociation rate constant (kd) of -3 -1 1.0 ± 0.07 x 10 s , resulting in a dissociation equilibrium constant (KD) of 5.0 ± 0.1 µM. The presence of + 2+ isocitrate, NADP and Mg increased the affinity of IcdS104P to Mdh 33-fold with a KD-value of 0.15 ± 0.03 µM, 3 -1 -1 because the association of the complex is faster (ka = 1.7 ± 0.7 x 10 M s ) and the dissociation is slower (kd = -4 -1 2.6 ± 0.6 x 10 s ). Remarkably, the enzymatic activity of wildtype Icd doubled, if it was in complex with Mdh. In contrast, the activity of Mdh was not affected in the presence of Icd or IcdS104P. The higher enzymatic activity of Icd in the presence of Mdh leads to an overproduction of α-ketoglutarate, since the activity of Mdh does not change. This might be a response to signal high anabolic demands triggered by high availability of + carbon sources and NADP . Excess of α-ketoglutarate can be applied to amino acid anabolism, since αketoglutarate is the precursor molecule of glutamate. No interaction was detected between Mdh and CitZ by SPR even in the presence of the corresponding metabolites. This result was surprising, because this interaction has already been characterized in other organisms. Moreover, the enzymatic reaction of Mdh, the endergonic conversion of malate to oxaloacetate, is only driven by the fast conversion of oxaloacetate by CitZ. Since no direct interaction between Mdh and CitZ was detected, other factors, e.g. other TCA cycle enzymes might contribute to their complex formation. Therefore, a hypothetic model of a TCA cycle metabolon including all TCA cycle enzymes was proposed in the end. The second part of this study deals with the carbon catabolite regulation (CCR), a mechanism which enables organisms the selective use of the carbon source, which allows the fastest growth. In B. subtilis, it is mediated by binding of the catabolite control protein A (CcpA) in complex with its coeffector HPrSer46P to its operator sites, the catabolite responsive elements (cres). Transcription of catabolic genes like gntR, xylA and xynP and of genes involved in overflow metabolism, e.g. ackA is regulated by CcpA, however, the regulation factors vary. To elucidate the impact of the cre-sequence on the regulation, in this study the kinetics and affinities of the interaction between the CcpA-HPrSer46P complex and these cre-elements were analyzed by SPR. Although the cre-elements have only small conserved parts, the CcpA-HPrSer46P complex bound to ackA-cre2, gntR-credown, syn-cre and xylA-cre with almost the same affinity with a KD-value of 1.6-3.8 nM. Interestingly, the affinity of the CcpA-HPrSer46P complex to xynP-cre is up to 19-fold higher with a KD of only 0.2 ± 0.1 nM. This higher affinity explains the high regulation factor and the tighter regulation of xynP by CcpA in contrast to gntR, xylA and ackA. -2- Introduction 3 Introduction 3.1 The Gram-positive model organism Bacillus subtilis Bacillus subtilis is a Gram-positive rod shaped non-pathogenic soil bacterium which belongs to the Firmicutes (Hoch et al., 1993). It is closely related to pathogenic Bacillus species and other Gram positive human pathogens, e.g. Staphylococcus aureus or Listeria monocytogenes (Alcaraz et al., 2010; Glaser et al., 2001; Kuroda et al., 2001). Therefore, it is an ideal model organism for molecular biological and medical research. The bacterium is forced to adapt quickly to environmental changes, e.g. heat stress, osmotic stress or availability of carbon sources (Görke & Stülke, 2008; Stülke & Hillen, 2000). There are different strategies to master these challenges, e.g. formation of endospores to survive unfavourable environmental conditions (Piggot & Hilbert, 2004) or synthesis of antibiotics to hinder growth of competing microorganisms (Kleerebezem, 2004; Stein, 2005). The organization of these biosyntheses demands tight regulation at genomic and proteomic level. One possibility of organization of enzymes, the formation of metabolons will be introduced in the next chapters. Hereinafter, the carbon catabolite regulation (CCR) will be presented as example for the regulation of gene expression depending on varying carbon sources. 3.2 Metabolon formation in B. subtilis Metabolic pathways represent series of reactions which either degrade molecules to gain energy, e.g. glycolysis or the tricarboxylic acid cycle (TCA cycle) or construct molecules under ATP consumption, e.g. the synthesis of amino acids. Until today, about 280 different pathways with up to 1300 reactions have been detected in B. subtilis, e.g. in central carbon metabolism, in nitrogen metabolism or in nucleotide anabolism (Barbe et al., 2009; Caspi et al., 2012; Goelzer et al., 2008; Lammers et al., 2010; Mäder et al., 2012). This high number of pathways suggests an organization of associated enzymes in multienzyme complexes, which are named metabolons (Srere, 1985). The advantage of a metabolon is that the interaction of enzymes catalyzing subsequent reactions allows the fast transfer of their intermediates. In silico studies revealed an increased synthesis rate for pyruvate, if glycolytic enzymes are organized in metabolons (Amar et al., 2008). A typical example of a metabolon of B. subtilis is the multienzyme complex of the RNA degradosome. It comprises the -3- Introduction helicase CshA to unwind secondary RNA structures, the RNAses J1 and J2 and the polynucleotide phosphorylase PnpA for RNA degradation. The whole complex is anchored at the cytoplasmic membrane by RNAse Y and, interestingly, this complex is also associated with the glycolytic enzymes phosphofructokinase Pfka and the enolase Eno (Commichau et al., 2009; Lehnik-Habrink et al., 2012; Lehnik-Habrink et al., 2010; Newman et al., 2012). A similar structure was determined in E. coli as well (Carpousis, 2007; Nurmohamed et al., 2010). It is assumed, that enzymes of other metabolic pathways are organized in metabolons as well. Recently, a multienzyme complex of TCA cycle enzymes was detected in vivo (Meyer et al., 2011). 3.2.1 TCA cycle as central part of metabolism and anabolism The TCA cycle is a series of eight reactions which oxidize the acetyl group of acetyl-CoA to CO2 and transfer the electrons to NADH, NADPH and FADH2 which conserve the electrons at a high electron transfer potential. These reducing equivalents are then reoxidized during the respiratory chain to gain ATP. The cycle starts by formation of citrate (C6), from oxaloacetate (C4) and acetyl-CoA (C2). Subsequently, acetyl-CoA is oxidized to two molecules of CO2, the remaining C4-unit Succinyl-CoA is converted to oxaloacetate and the cycle is closed (Baldwin & Krebs, 1981; Krebs & Johnson, 1980). An overview of all reactions is shown in Figure 3.1. In addition to catabolism of acetyl-CoA the TCA cycle provides precursor molecules for anabolism, e.g. α-ketoglutarate for glutamate synthesis or oxaloacetate which can be converted to amino acids of the aspartate family (Belitsky, 2002; Gunka & Commichau, 2012; Owen et al., 2002; Sonenshein, 2007). Moreover, oxaloacetate is used during gluconeogenesis to form glucose (Meyer & Stülke, 2012; Sauer & Eikmanns, 2005). Interestingly, in vivo interaction analyses revealed that almost every TCA cycle enzyme interacts with at least one other enzyme of this metabolic pathway (Meyer et al., 2011). The core complex of a TCA cycle metabolon might be formed by the isocitrate dehydrogenase Icd, the malate dehydrogenase Mdh and the citrate synthase CitZ, since their interaction was detected by various Strep protein interaction experiments (SPINEs) and by bacterial two hybrid assays (Meyer et al., 2011). -4- Introduction Figure 3.1: Overview of all TCA cycle reactions in B. subtilis. The metabolic intermediates are written in black. The enzyme names are highlighted in green, the reducing equivalents in blue color. The B. subtilis specific abbreviations of the enzyme names are marked with black boxes. Red arrows indicate the connections between TCA cycle and amino acid and nucleotide anabolism. 3.2.2 Regulation of mdh, icd and citZ In B. subtilis, the genes citZ, icd and mdh form an operon which encodes the citrate synthase (CitZ), the isocitrate dehydrogenase (Icd) and the malate dehydrogenase (Mdh) (Figure 3.2). All three genes are transcribed from the citZ promoter (Jin & Sonenshein, 1994b; Kim et al., 2002). If glucose or citrate are available as carbon sources, the expression of the whole operon is repressed by CcpA and CcpC (Jourlin-Castelli et al., 2000; Kim et al., 2002). Nevertheless, the basal activity of the citZ promoter is quite high in comparison to promoters which are stronger repressed by CcpA, e.g. the xynP promoter (Fischer, 2008). This might be due to the central CA pair in the citZ-cre-element which is not optimal for recognition by the CcpA-HPrSer46P complex (Kim et al., 2002; Schumacher et al., 2011). Since all three genes can be co-transcribed, they might form a complex during co-translation -5- Introduction (Jin et al., 1996; Jin & Sonenshein, 1994a, 1994b). Interestingly, transcription of icd is also controlled by a gene specific promoter which is not regulated by CcpA or CcpC (Jin et al., 1996; Jin & Sonenshein, 1994b; Kim et al., 2002). In exponential growth phase, equal amounts of full length transcript and of the shorter icd transcript were detected (Jin & Sonenshein, 1994b). The higher icd transcript levels correlate with the intracellular Icd protein amounts, because during exponential growth, the amounts of Icd and Mdh are twofold higher than the amount of CitZ (Maass et al., 2011). These data suggest, that CitZ might be uncoupled of the Mdh-Icd complex formation or that CitZ is not complexed in a 1:1 stoichiometry. Figure 3.2: Organization of citZ, icd and mdh in an operon. The genes are depicted by red arrows. Promoter sites (-35/-10) are shown in blue. The binding site of the transcriptional regulators CcpA (cre) or CcpC are highlighted in green or yellow, respectively. The ribosomal binding sites (RBS) are indicated in orange. (Picture based on the following references: (Jin & Sonenshein, 1994b; Jourlin-Castelli et al., 2000; Kim et al., 2002)). -6- Introduction 3.2.3 The NADP+ dependent isocitrate dehydrogenase Icd Icd (EC 1.1.1.42) belongs to the metal-dependent decarboxylating dehydrogenases and catalyzes the conversion of isocitrate to oxalsuccinate and finally to α-ketoglutarate (Figure 3.3) (Aktas & Cook, 2009; Singh et al., 2001). 0 0 NADP+ isocitrate CO2 NADPH oxalsuccinate α-ketoglutarate Figure 3.3: Icd catalyzes the oxidative decarboxylation of isocitrate. The enzymatic reaction is a two-step process comprising the oxidation of isocitrate to oxalsuccinate followed by the decarboxylation of oxalsuccinate to form α-ketoglutarate. The protein consists of 423 amino acids and has a molecular mass of 46.3 kDa. The active enzyme is organized as a homodimer like the almost structurally identical E. coli enzyme (Hurley et al., 1991; Singh et al., 2001). The Icd monomer is subdivided in three subdomains: the large domain containing N- and C-terminus and the small domain. The clasp domain is only formed in the dimer and serves as connection between the two monomers via hydrophobic interaction (Singh et al., 2001). The active site is formed by residues of both subunits and located at the cleft between large and small domain (see Figure 3.4). In the corresponding E. coli enzyme, Ser113, Asn115, Lys100 and Gln336 anchor NADP+ and isocitrate at the active site via H-bonds (Gonçalves et al., 2012; Singh et al., 2001). The activity of the enzyme is dependent on a bivalent cation, usually Mg2+ or Mn2+, in contrast, Ca2+ arrests Icd in its active conformation by blocking the active site (O'Leary & Limburg, 1977; Stoddard et al., 1993; Yasutake et al., 2003). As a consequence of the high structural identity, the E. coli and the B. subtilis enzyme reveal almost the same enzymatic kinetics with a Michaelis-Menten constant (Km) of 5.9 ± 0.9 µM (B. subtilis) and 4.9 ± 0.2 µM (E. coli) for isocitrate and a Km value of 14.5 ± 2.2 µM (B. subtilis) and 19.6 ± 3.7 µM (E. coli) for NADP+. The maximal velocity (vmax) of both enzymes is almost identical as well with 107 ± 7 nmol/min/mg enzyme (B. subtilis) or 135 ± 7 nmol/min/mg enzyme (E. coli), respectively (Singh et al., 2002). -7- Introduction Figure 3.4: Crystal structure of B. subtilis Icd dimer (Singh et al., 2001). The Icd monomers are depicted in yellow and blue. The active site of each monomer is located in the cleft between large and small domains (RCSB code: 1HQS). The enzymatic activity of the E. coli enzyme is regulated by phosphorylation of serine residue 113 by the isocitrate dehydrogenase kinase/phosphatase AceK, whereas the phosphorylated form is catalytically inactive (Borthwick et al., 1984; Cozzone & El-Mansi, 2005; Dean et al., 1989; LaPorte & Koshland, 1982). There is no AceK homologue in B. subtilis (Singh et al., 2001; Singh et al., 2002), but it was shown, that the enzyme can be phosphorylated by the B. subtilis protein kinase PrkC. However, the mechanism of this regulation is not completely characterized (Eymann et al., 2007; Lévine et al., 2006; Pietack et al., 2010). 3.2.4 The malate dehydrogenase Mdh As mentioned above, Icd is co-expressed together with the malate dehydrogenase Mdh (EC 1.1.1.37). The latter enzyme catalyzes the last reaction of the TCA cycle, the conversion of malate to oxaloacetate (Figure 3.5). It represents the only endergonic reaction of the TCA cycle and only the immediate processing of oxaloacetate by the following citrate synthase CitZ enables this reaction (Miller & Smith-Magowan, 1990; Ochoa, 1955). For malate as a substrate, the Michaelis-Menten constant (Km) of 900 µM is 14.8-fold higher than the Km value for oxaloacetate. In addition, the Km value for NAD+ (140 µM) as corresponding cofactor is also 5.1-fold higher than for NADH (27 µM) (Yoshida, 1965). Therefore, the activity of the enzyme is determined frequently by analyzing the backward reaction (Luo et al., 2006; Yin & Kirsch, 2007). -8- Introduction 0 NAD+ malate NADH oxaloacetate + Figure 3.5: Mdh catalyzes the NAD dependent oxidation of malate to oxaloacetate. The enzyme consists of 312 amino acids and has a molecular weight of 33.5 kDa. Until today, the crystal structure has not been solved. The structure of the corresponding E. coli enzyme shows that the amino acid residues Arg87, Asn119 and Arg153 coordinate the substrate at the active site. These residues are also conserved in the B. subtilis enzyme and therefore, its active site might be organized in a similar way (Hall & Banaszak, 1993; Hall et al., 1992; Hall et al., 1991). In addition, B. subtilis has four other malic enzymes, which catalyze the conversion of malate to pyruvate: MaeA, MalS, MleA and YtsJ. However, Mdh is the only enzyme which is able to convert malate to oxaloacetate. The conversion of oxaloacetate to phosphoenolpyruvate (PEP) is the entry of the gluconeogenesis and, as a consequence, Mdh is essential for growth of cultures with malate as sole carbon source (Lerondel et al., 2006; Meyer & Stülke, 2012). 3.2.5 The citrate synthase CitZ After regeneration of oxaloacetate by Mdh, the TCA cycle starts again with the first reaction, the condensation of oxaloacetate and acetyl-CoA to citrate catalyzed by CitZ (372 amino acids, 41.7 kDa) (Jin & Sonenshein, 1996). The reaction is depicted in Figure 3.6. The Km value for acetyl-CoA is 622 µM and for oxaloacetate 7-15 µM. The high efficiency of CitZ and its high substrate specificity for oxaloacetate and acetyl-CoA likely hinder the backward reaction of Mdh (Jin & Sonenshein, 1996; Wiegand & Remington, 1986). Mdh and CitZ are co-expressed, when they are transcribed from the same promoter and therefore, they might form a complex in situ nascendi (Jin & Sonenshein, 1994b). For citrate synthases of other species, e.g. from pig heart mitochondria, the interaction with the malate dehydrogenase was already detected and quantified (Datta et al., 1985; Halper & Srere, 1977; Morgunov & Srere, 1998; Tompa et al., 1987b). -9- Introduction + 0 H2O CoA-SH acetyl-CoA citrate oxaloacetate Figure 3.6: CitZ catalyzes the condensation of acetyl-CoA and oxaloacetate to citrate. Although this enzyme plays an important role in the TCA cycle, surprisingly less is known about its structure which has not been solved until today. Surprisingly, Gram-positive citrate synthases share high amino acid sequence identity with archaeal and eukaryotic citrate synthases, e.g. the citrate binding amino acids His222, His262 and Asp317 of the thermophilic archeon Thermoplasma acidophilum are conserved in the B. subtilis enzyme as well (Russell et al., 1994). Similar results were obtained from alignments with the citrate synthase of the hyperthermophilic archeon Pyrococcus furiosus and with the eukaryotic variant from chicken heart (Liao et al., 1991; Remington et al., 1982; Russell et al., 1997). These amino acid homologies suggest that the active site of the B. subtilis enzyme might be organized in a similar conformation. In summary, the interaction between Icd, Mdh and CitZ was detected in vivo (Meyer et al., 2011). However, nothing is known about the dynamics and the conditions of this complex formation and if the interaction has an impact on the activity of the enzymes. Since all three genes are organized in one operon, this complex might be formed during co-expression (Jin et al., 1996; Jin & Sonenshein, 1994b). The expression of this operon is repressed in the presence of glucose by the global regulator CcpA (Kim et al., 2002). The general mechanism of CcpA dependent carbon catabolite regulation will be presented in more detail in the following chapters. - 10 - Introduction 3.3 Regulation of carbon metabolism of B. subtilis The carbon catabolite regulation (CCR) allows a bacterium selectively to use only the carbon sources which allow the fastest growth. For B. subtilis, the most favored carbon sources are glucose and malate (Kleijn et al., 2010; Stülke & Hillen, 2000). The presence of glucose leads to repression of genes encoding enzymes involved in catabolism of secondary sugars. On the other hand, the expression of enzymes which metabolize glucose is activated (Deutscher, 2008; Deutscher et al., 2006; Gunnewijk et al., 2001). A central source for signaling in CCR is the phosphoenolpyruvate: sugar phosphotransferase system (PEP:PTS) which will be introduced in more detail in the following chapter. 3.3.1 The phosphoenolpyruvate: sugar phosphotransferase system (PEP:PTS) The PEP:PTS transports glucose and other so called “PTS-sugars”, e.g. fructose, mannose, maltose or the sugar alcohols mannitol and glycerol into the cell (Cases et al., 2007; Deutscher et al., 2006; Postma et al., 1993; Reizer et al., 1999; Singh et al., 2008). The dephosphorylation of the catabolic intermediate phosphoenolpyruvate (PEP) delivers the energy for the uptake of the sugars. The phosphoryl group is translocated to enzyme I (EI) and subsequently to the hisitidine containing protein HPr, which is phosphorylated at the amino acid residue histidine 15. Finally, the phosphoryl group is transferred to the sugar specific enzyme complex II (EIIA and EIIB) which leads to phosphorylation of the uptaken sugar, e.g. glucose is taken up as glucose-6-phosphate (Glc-6-P) which directly enters into the glycolysis allowing a fast gain of energy for the cell (Postma et al., 1993; Stülke & Hillen, 2000). - 11 - Introduction Figure 3.7: Scheme of the PEP:PTS in B. subtilis. Dephosphorylation of Phoshoenolpyruvate (PEP) leads to phosphorylation of Enzyme I (EI). Afterwards the phosphoryl group (written in bold) is transferred to His15 Glc of HPr which transfers it to Enzyme II domain A. (EIIA ). Glc This domain phosphorylates EII B (EIIB ) which passes subsequently the phosphoryl group to glucose to the glucose to form glucose-6-P. CM: cytoplasmic membrane. Picture based on Stülke & Hillen (Stülke & Hillen, 2000). 3.3.2 The catabolite control protein A and CCR in B. subtilis In B. subtilis and other Firmicutes, e.g. in the pathogens Bacillus anthracis, Clostridium difficile, Clostridium perfringens or Staphylococcus aureus, the catabolite control protein A (CcpA) is the central regulator of CCR (Antunes et al., 2012; Chiang et al., 2011; Fujita, 2009; Seidl et al., 2008; Seidl et al., 2009; Stülke & Hillen, 2000; Varga et al., 2004; Varga et al., 2008). It regulates the expression of approximately 10 % of genes whose gene products are involved in central physiological processes, e.g. carbon metabolism, amino acid metabolism, sporulation and biofilm formation (Chagneau & Saier Jr, 2004; Fujita, 2009; Lorca et al., 2005; Lulko et al., 2007; Sonenshein, 2007; Stanley et al., 2003). In addition, CcpA regulates the expression of virulence factors in the pathogen species, e.g. AtxA in B. antracis or SpeB in Streptococcus pyogenes (Chiang et al., 2011; Kietzman & Caparon, 2010; Seidl et al., 2006; Shelburne et al., 2008). CcpA belongs to the LacI/GalR family and is a 334 amino acids protein with a total molecular weight of 36.9 kDa. (Chambliss, 1993; Hueck et al., 1995; Weickert & Adhya, 1992). The crystal structure revealed two main domains of the protein: The core protein, which can be subdivided into an N- and C-terminal subdomain, forms the dimerization domain and the corepressor binding domain and the N-terminal DNA binding domain (Figure 3.8). Strikingly, the DNA binding domain is only in close - 12 - Introduction contact with the N-terminal subdomain, if it is bound to its operator sequence. The core protein exhibits an open conformation, if it does not interact with its operator DNA (Schumacher et al., 2004; Schumacher et al., 2011; Singh et al., 2007). Figure 3.8: Crystal structure of CcpA-(HPrSer46P)-syn cre ternary complex according to Schumacher (Schumacher et al., 2011). The CcpA subunits are depicted in black and blue. The two molecules of HPrSer46P are shown green and yellow. The synthetic cre-element (syn-cre) is represented in white. A: front view of the complex, B: 90 rotated view. (RCSB code: 3OQO). If a favoured carbon source, e.g. glucose, is available, it is metabolized via glycolysis and therefore, the intracellular concentration of fructose-1.6-bisphosphate (FBP) is high. This stimulates the activity of the HPr kinase/phosphorylase (HPrK/P) which phosphorylates HPr at serine 46, its second phosphorylation site (Deutscher & Saier Jr, 2005; Galinier et al., 1998; Jault et al., 2000; Nessler et al., 2003; Ramström et al., 2003). Under nutrient limiting conditions, HPrK/P changes its activity. If the levels of FBP and Glc-6-P are low and the intracellular amount of inorganic phosphate (Pi) is high, HPrK/P desphosphorylates HPrSer46P (Mijakovic et al., 2002). In addition to HPr, the catabolite repression like HPr protein Crh is phosphorylated by HPrK/P as well (Galinier et al., 1997). This protein has no function in the PEP:PTS, since it carries a glutamine in position 15 instead of a histidine (Galinier et al., 1997). Although CrhSer46P is able to bind to CcpA (Schumacher et al., 2006), the contribution to CCR might be only an additional function of Crh, since the unphosphorylated protein binds to the methylglyoxylal synthase MgsA and inhibits its function in the methyl glyoxal pathway (Landmann et al., 2011). After phosphorylation at Ser46, two molecules of either HPrSer46P or CrhSer46P bind to a CcpA dimer. The binding of the coeffectors induces several conformational changes which result in the formation of the hinge helices and orientation of the HTHLH motifs in the DNA binding conformation - 13 - Introduction (Schumacher et al., 2004; Schumacher et al., 2011). Moreover, the interaction between HPrSer46P and CcpA is stimulated by the low molecular weight effectors FBP and Glc-6-P (Seidel et al., 2005). In contrast to HPrSer46P, the interaction of CcpA and CrhSer46P is not stimulated by FBP and Glc-6-P (Horstmann et al., 2007; Seidel et al., 2005). The CcpA-coeffector complex is able to bind to its operator sequences, the catabolite responsive elements (cre) and regulate transcription of the corresponding genes (Görke & Stülke, 2008; Titgemeyer & Hillen, 2002; Warner & Lolkema, 2003). Figure 3.9: Carbon catabolite regulation (CCR) in B. subtilis. HPr (green circle) with its two phosphorylation sites represents the link between glucose uptake and CCR. HPrHis15P is part of the PEP-PTS (depicted at the top of the scheme). If glucose is available, it will be metabolized in glycolysis. As a consequence, the intracellular amount of fructose-1,6-bisphosphate (FBP) rises, which leads to an activation of the HPr kinase phosphorylase (HPrK/P, blue circle). HPrK/P phosphorylates HPr or Crh (orange rhomb) at serine 46. HPrSer46 or CrhSer46P, respectively, bind to CcpA (yellow) and this ternary complex binds its operator elements (cresites) in the genome. The binding of CcpA-HPrSer46 to the cre- element can cause either activation or repression of transcription, depending on the location of the cre-element in reference to the promoter. HPrK/P dephosphorylates HPrSer46P if (1) high amounts of inorganic phosphate (P i) and (2) low amounts of ATP are available. CM: cytoplasmic membrane (picture based on Görke & Stülke (Görke & Stülke, 2008). - 14 - Introduction 3.3.3 The catabolite responsive element cre CcpA-HPrSer46P regulates transcription of its target genes by binding to its operator sites, the creelements (Fujita, 2009). They are approximately 14-16 basepairs (bps) long and the sequence is almost palindromic. Leu 56 of CcpA, the “leucine lever” interacts with the central CG in the minor groove of the DNA and causes a kink of the DNA. As a consequence, the minor groove expands around the central CG pair and allows the binding of CcpA (Schumacher et al., 2004; Schumacher et al., 2011). Due to the high flexibility of the helix turn helix (HTH) motif of the DNA binding domain, CcpA is able to recognize highly degenerated DNA motifs with only small conserved parts (Table 3.1). Table 3.1: Comparison of cre consensus sequences Reference Consensus sequence (5’→3’) (Weickert & Chambliss, 1989) T G W N A N C G N T N W C A (Hueck et al., 1994) W G N A A S C G N W W N C A (Miwa et al., 2000) W W T G N A A R C G N W W W C A W W W T G N A A R C G Y T T W W N (Miwa & Fujita, 2001) Conserved regions are written in bold, red letters. W: A/T R: G/A S: C/G Y: C/T N: A/C/G/T The position of the cre-site in reference to the promoter leads to either activation or repression of transcription. Cre-sequences located downstream or within the promoter region mainly cause repression of transcription, e.g. the cre-sequences of xylA, xynP or gntR-credown (Galinier et al., 1999; Jacob et al., 1991; Kraus et al., 1994; Miwa & Fujita, 1990; Miwa et al., 1997). The cre-sequences of ackA are located upstream of the promoter region and transcription is activated by CcpA (Grundy et al., 1993; Moir-Blais et al., 2001). In the next chapters, the regulation of these four genes will be introduced in more detail as these belong to three different groups of genes regulated by CcpA: (1) genes whose transcription is activated in the presence of CcpA, e.g. ackA; (2) genes whose transcription is repressed moderately by CcpA, e.g. gntR and (3) genes whose transcription is repressed strongly by CcpA, e.g. xylA and xynP (Marciniak et al., 2012). - 15 - Introduction 3.3.4 The regulation of ackA, gntR, xylA and xynP During overflow metabolism, AckA, the acetate kinase, converts glucose to acetate in combination with the phosphotransacetylase Pta. This represents two advantages for the cell: Accumulation of acetyl-CoA is prevented and the pH of the environment is lowered and therefore, the growth of other bacteria is hindered (Grundy et al., 1993; Presecan-Siedel et al., 1999). The expression of ackA is activated by several different regulators: The binding of the CcpA-HPrSer46P complex to ackAcre2, located upstream of the promoter region, activates transcription of the gene 20-fold in the presence of glucose (Sprehe et al., 2007; Turinsky et al., 1998). In addition, the transcriptional regulator CodY cooperatively activates expression of ackA. The corresponding binding sites are located upstream of ackA-cre2 (Belitsky & Sonenshein, 2008; Moir-Blais et al., 2001; Shivers et al., 2006). As both regulators are able to interact with RpoA, the α-subunit of the RNA polymerase, they probably fix the RNA polymerase in the optimal position to start the transcription (Wünsche et al., 2012). GntR is the repressor of the gntRKPZ operon. This operon encodes the enzymes GntK (gluconate kinase), GntP (gluconate permease) and GntZ (NAD+-6-phosphogluconate dehydrogenase) which catalyze the uptake and metabolization of gluconate (Fujita et al., 1986; Miwa & Fujita, 1988; Reizer et al., 1991). In the presence of gluconate, GntR is relieved from its operator binding site and the operon is expressed (Dowds et al., 1978; Nihashi & Fujita, 1984; Yoshida et al., 1995). In presence of glucose, the expression of these genes is repressed 11-fold by CcpA (Sprehe et al., 2007). The operon contains two cre-elements, creup located within the -35 region and credown located within the coding region of gntR. Only credown is bound by the CcpA-HPrSer46P complex. In contrast, creup is bound by only by CcpA in combination with glucose-6-phosphate (Fujita & Miwa, 1994; Fujita et al., 1995; Miwa & Fujita, 1990; Miwa et al., 1997). Additionally, CcpB regulates gntRKPZ expression instead of CcpA under oxygen limited conditions (Chauvaux et al., 1998). Xylan and xylose serve as secondary carbon sources. They are taken up by AraE (xylose) and XynP (xylan) (Krispin & Allmansberger, 1998; Lindner et al., 1994) and are metabolized by the enzymes encoded by the xylAB and xynPB operons. The expression of these two operons is induced in the presence of xylose by the inactivation of the regulator XylR (Gärtner et al., 1992; Gärtner et al., 1988; Lindner et al., 1994). If glucose is available, the expression of both operons is repressed by the CcpAHPrSer46P complex: The expression of xylAB is reduced 38-fold, the expression of xynPB 40 to 440fold depending on the utilized medium (Galinier et al., 1999; Jacob et al., 1991; Kraus et al., 1994; Sprehe et al., 2007; Steinert, 2011). In addition, high intracellular amounts of glucose-6-phosphate - 16 - Introduction have an anti-inducing effect on XylR. As a consequence, XylR is not released from its operator DNA even if the inducer xylose is available, if the energy level of the cell is high (Dahl et al., 1995). The transcription of all genes presented in this chapter is regulated by CcpA, however, the strength of the regulation varies. The interaction between CcpA-HPrSer46P and xylA-cre of B. megaterium was quantified already and revealed a KD-value of 0.6 ± 0.3 nM (Seidel et al., 2005). However, for B. subtilis, the interaction of the CcpA-HPrSer46P complex with cres of strongly or moderately repressed genes, e.g. xynP or gntR, respectively, or genes activated by CcpA, e.g. ackA has never been characterized so far. 3.4 Scope of the thesis (1) The interaction of the TCA cycle enzymes Mdh, Icd and CitZ has already been detected in vivo. However, the dynamics of this complex formation remain unclear so far. This study addresses the question, how this complex is formed in vitro and if its formation is influenced by certain metabolites. Since the formation of a metabolon enables the direct contact of particular enzymes and therefore, the fast transfer of their intermediates, the second part of this study aims to elucidate the influence of the interaction partners on the enzymatic activity of the single enzymes. (2) The expression of ackA, gntR, xylA and xynP is regulated by CcpA, however, the strength of this regulation varied. Since the cre-elements of these genes are highly degenerated sequences which have only very small conserved parts, they might be bound with different affinities by the CcpA-HPrSer46P complex. This part of the study aims to elucidate the impact of the cre-sequences on the repression and activation of expression by determining the kinetics of the CcpA-HPrSer46P interaction with various cre-elements in vitro. - 17 - Material and Methods 4 Material and Methods 4.1 Material 4.1.1 Chemicals The chemicals used for this thesis were purchased from Merck (Darmstadt), Roth (Karlsruhe) or Sigma (Munich) in p.a. quality. All chemicals are summarized in Table 4.1. The following tables content the used auxiliary materials (Table 4.2), instruments (Table 4.3), reagent mixtures (Table 4.4), enzymes, proteins and standards (Table 4.5) and Oligonucleotides (Table 4.6). Table 4.1: Chemicals Chemical Source 2-Nitrophenol-β-D-galactopyranosid (ONPG) Acrylamide, Rotiphorese gel (19:1) Acrylamide, Rotiphorese gel (37:1) Adenosintriphosphate-Monohydrate (ATP) Agar Agarose Ammoniumperoxodisulfate (APS) Ampicillin Bactotryptone Boric acid Bovine serum albumine Bradford reagent Bromphenolblue Calciumchloride, Dihydrate Casein acid hydrolysate (CAA) Chloramphenicol Chloroform Complete-proteaseinhibitor, EDTA-free Coomassie Brillant Blue Applichem, Darmstadt Roth, Karlsruhe Roth, Karlsruhe Roche, Mannheim Merck, Darmstadt Peqlab, Erlangen Merck, Darmstadt Roth, Karlsruhe Oxoid, Hampshire, GB Merck, Darmstadt Roth, Karlsruhe BioRad, Munich Roth, Karlsruhe Sigma, Steinheim Sigma, Munich Serva, Heidelberg Roth, Karlsruhe Roche, Mannheim Amersham Pharmacia Biotech, Sweden Roth, Karlsruhe Peqlab, Erlangen Sigma, Steinheim Sigma, Munich Roth, Karlsruhe Merck, Darmstadt Roth, Karlsruhe Roth, Karlsruhe Sigma, Steinheim D-(+)-Glucose, Monohydrate Desoxynucleotidetriphosphates (dNTPs) Desthiobiotin Dimethylformamide Dimethylsulfoxide (DMSO) Dipotassiumhydrogenphosphate Disodiumhydrogenphosphate Dithiothreitol DL-Isocitric acid trisodium salt hydrate - 18 - Material and Methods Chemical Source Erythromycin Ethanol, absolute Ethanol, technical Ethidium bromide Ethylendiamintetraaceticacid-disodiumsalt, Dihydrate (EDTA) Ferric-ammonium-citrate (CAF) Formamid Fructose-1,6-bisphosphate, Octahydrate Glycerol Glycerol Glycine HEPES Hydrochloric acid Imidazol Iodonitrotetrazolium chloride Isopropylalcohol Isopropyl-β-D-galactopyranoside (IPTG) Kanamycin L-(-) Malic acid L-Glutamate L-Isoleucine L-Leucine L-Methionine L-Tryptophan L-Valine Magnesium chloride, Hexahydrate Magnesium sulfate Manganese (II) sulfate, Monohydrate Methanol N,N,N’,N’- Tetramethylenediamine (TEMED) NeutrAvidin TM Oxaloacetic acid Paraformaldehyde Phenazine methosulfate Phenazine methosulfate (PMS) Phenylmethylsulfonyfluoride (PMSF) p-Iodonitrotetrazolium-Violet (INT) Polyethylenglycol (PEG) 6000 Potassium chloride Potassium dihydrogen phosphate Potassium hydroxide Rotenone Sodium acetate Sodium carbonate Sodium chloride Sodium dihydrogenphosphate Sodium dodecylsulfate Sodium hydroxide Succinic acid, sodium salt Sigma, Steinheim Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Sigma, Steinheim Merck, Darmstadt Sigma, Heidelberg Fluka, CH Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Merck, Darmstadt Sigma, Steinheim Merck, Darmstadt Roth, Karlsruhe Roth, Karlsruhe Sigma, Steinheim Sigma, Steinheim Roth, Karlsruhe Sigma, Steinheim Sigma, Steinheim Sigma, Steinheim Sigma, Steinheim Sigma, Steinheim Roth, Karlsruhe Fluka, CH Merck, Darmstadt Roth, Karlsruhe Pierce, USA Sigma, Steinheim Roth, Karlsruhe Sigma, Steinheim Sigma, Steinheim Roth, Karlsruhe Sigma, Steinheim Sigma, Munich Merck, Darmstadt Roth, Karlsruhe Peqlab, Erlangen Sigma, Steinheim Merck, Darmstadt Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Roth, Karlsruhe Serva, Heidelberg Serva, Heidelberg - 19 - Material and Methods Chemical Source Tetracycline-Hydrochloride Triethanolamine hydrochloride Tris(hydroxymethyl)ammoniummethane (Tris) Trisodiumcitrate, Dihydrate TritonX-100 Tryptone Tween 20 Xylene cyanol Yeastextract α-Ketoglutaric acid sodium salt β-Mercaptoethanol β-Nicotinamide adenine dinucleotide hydrate 2-phosphate, reduced tetrasodium salt hydrate (NADPH) β-Nicotinamide adenine dinucleotide hydrate from Yeast (NAD+) β-Nicotinamide adenine dinucleotide phosphate hydrate (NADP+) β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate (NADH) Merck, Darmstadt Sigma, Steinheim Roth, Karlsruhe Sigma, Steinheim Roth, Karlsruhe Oxoid, GB Sigma, Munich Roth, Karlsruhe Roth, Karlsruhe Sigma, Steinheim Merck, Darmstadt Sigma, Steinheim Sigma, Steinheim Sigma, Steinheim Sigma, Steinheim 4.1.2 Auxiliary Material Table 4.2: Auxiliary Material Material Source Amino coupling Kit for Biacore X BIAmaintenance Kit for BiacoreX Centriprep Centrifugal filter devices (MWCO 3, 10, 30, 50 kDa) Chromatographypaper Chromatograpy material DEAE – Sephacel Chromatograpy material Fractogel EMD TMAE Chromatograpy material POROS 20 MC Chromatograpy material POROS HS/M Electroporation cuvettes Eppendorf reaction tubes Gene Amp reaction tubes Gilson tips Glas pipettes HBS-EP buffer Hiprep QFF sepharose column Hiprep SepharoseQ column HisTrap FF, NiNTA column Microliterpipets Nitrocellulose filter (0.45 μm pore size) GE Healthcare, Munich GE Healthcare, Munich - 20 - Millipore, USA Whatman, England Pharmacia, Freiburg Merck, Darmstadt Applied Biosystems, Weiterstadt Applied Biosystems, Weiterstadt EQUIBIO, Great Britain Greiner, Nürtingen Perkin Elmer, Weiterstadt Greiner, Nürtingen Brand, Wertheim GE Healthcare, Munich GE Healthcare, Munich Pharmacia, Freiburg GE Healtcare, Munich Gilson, Düsseldorf Sartorius, Göttingen Material and Methods Material Source Petridishes Polyvinylidenedifluoride (PVDF) membrane Quartzglass cuvettes Semi-micro cuvettes Sensorchip CM5 Sensorchip SA Size exclusion chromatography column Superdex G200 Size exclusion chromatography column Superdex G75 Slyde-A-Lyzer Dialysis Units Sterile filter Minisart (0.2 µM pore size) Superformance Glass column 150C-10 Ultrafiltration membranes MWCO 3 kDa XK-column Greiner, Nürtingen Pall Filtron, USA Hellma, Mühlheim Greiner, Nürtingen GE Healthcare, Munich GE Healthcare, Munich Amersham, Freiburg Amersham, Freiburg Pierce, Schleicher & Schüll, Dassel Merck, Darmstadt Millipore, USA Amersham, Freiburg 4.1.3 Instruments Table 4.3: Instruments Instrument Source Äkta FPLC Äkta Prime Analytical Balance BIAcoreX Biofuge A centrifuge Biofuge Fresco Branson Sonic Sonifier B12 Centrifuge J21B DNA Thermo Cycler 2400 Electrophoresis/Electroblotunit Mini V8-10 Electroporation Device Gene Pulser + Puls Controller FPLC chromatography system Microprocessor pH-Meter calimatic Mini-PROTEAN® Tetra Cell chamber Novaspec III visible photometer Rotary shaker G-25 Sequencer ABI 310 Genetic Analyzer Speed Vac centrifuge Stirred Ultrafiltration Cell Ultracentrifuge L7-55 Ultrospec photometer 3000, Novaspec Video documentation system VarioCam Pharmacia, Freiburg Pharmacia, Freiburg Sartorius, Göttingen BIAcore, Sweden Heraeus Christ, Osterode Heraeus Christ, Osterode Braun, Melsungen Beckmann, München Perkin Elmer, USA Gibco/BRL, Karlsruhe Biorad, München Pharmacia, Freiburg Knick, Berlin Biorad, Munich Amersham, Freiburg New Brunswick, Neu-Isenburg PE Applied Biosystems, Freiburg Bachofer, Reutlingen Amicon, USA Beckmann, München Pharmacia, Freiburg Biotec Fischer, Reiskirchen - 21 - Material and Methods 4.1.4 Reagent mixtures Table 4.4: Reagent mixtures Reagent Mixtures Usage Source NucleoBond® AX100 & AX500 Preparation of plasmid DNA Purification of DNA from agarose gels and enzymatic reactions Purification of plasmid DNA Purification of chromosomal DNA of B. subtilis Macherey-Nagel, Düren NucleoSpin® Gel and PCR clean up NucleoSpin® Plasmid QIamp DNA Mini Kit Macherey-Nagel, Düren Macherey-Nagel, Düren QIAGEN, Hilden 4.1.5 Proteins, enzymes and standards Table 4.5: Proteins, enzymes and standards Enzymes, proteins and standards Restriction endonucleases AgeI BamHI BlpI EcoRI HindIII NheI PmeI SacI XbaI XhoI XmaI New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach New England Biolabs, Schwalbach Standards PeqGold protein marker II PeqGold DNA Leitermix PeqGold DNA Leitermix I Ultra low range Peqlab, Erlangen Peqlab, Erlangen Peqlab, Erlangen Enzymes Calf intestine phosphatase (CIP) DNAseI Phusion DNA-polymerase Proteinase K RNAse A T4-DNA ligase T4-DNA quickligase TetR(B) and TetR(BD) New England Biolabs, Schwalbach Sigma, Steinheim Finnzymes, Finland Merck, Darmstadt Sigma, Steinheim New England Biolabs, Schwalbach New England Biolabs, Schwalbach Obtained from Stephanie Platzer and Dr. Gerald Seidel, Erlangen - 22 - Material and Methods 4.1.6 Oligonucleotides Table 4.6: Oligonucleotides Oligonucleotide citZfwNhe2 citZrevBam Sequence (5’-3’) Application CAGTGGCTAGCATGACAGCGACACGCGGTCTT GAAG GACTGGATCCTTAGGCTCTTTCTTCAATCGGAA CG Forward primer for cloning of citZ from B. subtilis Reverse primer for cloning of citZ from B. subtilis 5’ biotinylated for SPR analysis for SPR analysis 5’ biotinylated for SPR analysis for SPR analysis 5’ biotinylated for SPRanalysis for SPR analysis 5’ biotinylated for SPR analysis for SPR analysis 5’ biotinylated for SPR analysis for SPR analysis Sequencing primer for icd from B. subtilis Forward primer for cloning of icd from B. subtilis Sequencing primer for icd from B. subtilis Sequencing primer for icd from B. subtilis Reverse primer for cloning of icd from B. subtilis Sequencing primer for icd from B. subtilis Sequencing primer for mdh from B. subtilis Sequencing primer for mdh from B. subtilis Sequencing primer for mdh from B. subtilis Sequencing primer for mdh from B. subtilis 5’ biotinylated for SPR analysis for SPR analysis Forward sequencing primer for pET28b-derivatives Reverse sequencing primer for pET28b-derivatives cre2ackAfw TTCTTATTGTAAGCGTTATCAATACG cre2ackArev CGTATTGATAACGCTTACAATAAGAA cregntRfw GTCTGATTGAAAGCGGTACCATTTTA cregntRrev TAAAATGGTACCGCTTTCAATCAGAC creusfw26nt AATCATTTATGGCATAGGCAACAAGT creusrev26nt ACTTGTTGCCTATGCCATAAATGATT crexylAfw ACTATTTGGAAGCGCAAACAAAGTG crexylArev CACTTTGTTTGCGCTTCCAAAATAGT crexynPfw ACTGTTTTGAAAGCGCTTTTATAAAA crexynPrev TTTTATAAAAGCGCTTTCAAAACAGT Icdfw2781 GTTATTCTTTCAGGCGTTCTGC IcdfwBam IcdfwXho Icdrev1911 IcdrevXho IcdrevXma MdhfwXho MdhrevXma ATCGTCGGATCCTATGGCACAAGGTGAAAAAA TTACAGTC ATCGTCCTCGAGGTGGCACAAGGTGAAAAAAT TACAGTC TATTCGCGGATCACATCTAATGTTTC GTCCTGCTCGAGTTAGTCCATGTTTTTGATCAG TTCTTC GTCCTGCCCGGGTTAAGTCCATGTTTTTGATCA GTTC AGCGTACTCGAGCATGGGAAATACTCGTAAAA AAG TCTAGTGCCCGGGTTAGGATAATACTTTCATGA CATTTTTG MdhSeq3162rev GCTTGCTTCAAGCATATC Mdhseq3601 ATTCCGAAAGAACGGATTGA syncrefw TTCTTACTGTTAGCGCTTTCAGTACG syncrerev CGTACTGAAAGCGCTAACAGTAAGAA T7 Promotorprimer CGCGAAATTAATACGACTCACTATAGGG T7 Terminatorprimer GCTAGTTATTGCTCAGCGG - 23 - Material and Methods 4.2 Bacterial strains Table 4.7: Bacterial strains Strain Genotype Reference Bacillus subtilis 168 E. coli DH5α trp+ recA1 endA1 gyrA96 relA1 hsdR17(rkmk+) supE44 thiΦ80 ΔlacZΔM15 Δ(lacZYA-argF)U169 BL21(DE3) ΔptsH/crr/pLysS, KanR, CamR F- ompT hsdSB (rB-mB-) gal dcm (DE3) pLysS (CamR) (Zeigler et al., 2008) (Hanahan, 1985) E. coli FT1 E. coli BL21(DE3) pLysS (Parche et al., 1999) (Studier & Moffatt, 1986) 4.3 Plasmids Table 4.8: Plasmids Plasmid Characteristics Reference p4813 pET-28b(+) AmpR, ptsK KanR, oripBR322, overexpression vector including N-terminal His-tag pWH844 derivative: AmpR, overexpression of B. subtilis mdh with N-terminal His-tag pWH844 derivative: AmpR, overexpression of B. subtilis icdS104P mutant with N-terminal His-tag pET3c ptsH (B. subtilis) pET3c ccpAhis6 pET-28b(+), for overexpression of B. subtilis icd with N-terminal His-tag pET-28b(+), for overexpression of B. subtilis citZ with N-terminal His-tag (Reizer et al., 1998) Novagen pGP385 pGP389 pWH466 pWH653 pWH2410 pWH2421 - 24 - Unpublished, obtained from Jörg Stülke, Göttingen Unpublished, obtained from Jörg Stülke, Göttingen (Seidel et al., 2005) (Seidel, 2005) This work This work Material and Methods 4.4 Buffer and media If not stated differently, buffers, media and solutions were prepared with Millipore water or deionized water and autoclaved for 20min at 121°C. Solutions containing heat labile ingredients were filtered with a sterile filter (0.2 µM) and added to the autoclaved medium after cooling down to 50°C. 4.4.1 General buffers and solutions TE buffer SBT buffer 50 mM Tris/HCl pH 7.5 10 mM Tris/HCl pH 8 200 mM NaCl 0.1 mM EDTA 4.4.2 Buffers and solutions for protein purification All buffers used for chromatography were sterile filtered (0.2 µM pore size) and stored at 4°C. Buffers for anion exchange chromatography Buffer A: 10 mM Tris/HCl, pH 7.5, 2 mM DTT Puffer B: 10 mM Tris/HCl, pH 7.5, 2 mM DTT, 1 M NaCl Regeneration buffers: 2 M NaCl, 30 % (v/v) isopropanol, 1 M NaOH Buffers for NiNTA affinity chromatography HisA buffer: 50 mM NaH2PO4, 300 mM NaCl, 50-75 mM Imidazole HisB buffer: 50 mM NaH2PO4, 300 mM NaCl, 500 mM Imidazole Stripping buffer: 20 mM NaH2PO4, 500 mM NaCl, 50 mM EDTA, pH 7.4 100 mM NiSO4 for recharging the column Buffers for size exclusion chromatography HBS-E buffer: 150 mM NaCl, 3 mM EDTA, 10 mM HEPES (pH 7.4) 0.5 mM NaOH for regeneration of the column - 25 - Material and Methods 4.4.3 DNA and protein gel electrophoresis 4.4.3.1 DNA gel electrophoresis 50x TAE DNA loading dye 2M Tris 0.05 % (w/v) bromphenole blue 1 M NaOAc 0.05 % (w/v) xylene cyanole 62.5 mM EDTA 2 % (w/v) SDS pH 8.3 (glacial acetic acid) 1 mM EDTA 50 % (v/v) glycerol 2 % (v/v) 50x TAE DNA size standards: Peqlab DNA “Leitermix” 10/8/6/4/3.5/3/2.5/2/1.5/1.2/1/0.9/0.8/0.7/0.6/0.5/0.4/0.3/0.2/0.1 kbps Peqlab DNA “Leitermix I” Ultra low range 300/200/150/100/75/50/35/25/20/15/10 bps 4.4.3.2 Protein gel electrophoresis SDS-PAGE 10x SDS running buffer 5x Protein loading dye 1.92 M glycine 20 % (w/v) SDS 0.5 M Tris 60 % (v/v) glycerol 10 % (w/v) SDS 250 mM Tris pH 8.5-8.8 10 % (v/v) β-mercaptoethanol 0.05 % (w/v) Serva blue G-250 Protein size marker for SDS gel electrosphoresis: PeqGold protein marker II 200/150/120/100/85/70/60/50/40/30/25/20/15/10 kDa - 26 - Material and Methods Stacking gel Separation gel 4 % (v/v) acrylamide (37.5:1) 10 % (v/v) acrylamide (37.5:1) 85 mM Tris/HCl (pH 6.8) 85 mM Tris/HCl (pH 8.8) 0.1 % (w/v) SDS 100 mM Tris/ boric acid (pH 8.8) With H2O ad 9.95 ml 0.1 % (v/v) SDS 50 µl 10 % (w/v) APS With H2O ad 9.8 ml 9 µl TEMED 200 µl 10 % (w/v) APS 5 µl TEMED Native Gelelectrophoresis Separation gel 10x native protein PAGE buffer 7.5 % - 15 % (v/v) Acrylamide (37.5:1) 1.92 M glycine 85 mM Tris/HCl (pH 8.8) 0.5 M Tris 100 mM Tris/boric acid (pH 8.8) pH 8.8 With sterile H2O ad 10.6 ml 200 μl APS 5x native protein loading dye 9 μl TEMED 50 % (v/v) Glycerol 1.5 % (v/v) Bromphenol blue 5x TBE Native PAA gel (TBE) 450 mM Tris 4 % (v/v) Acrylamide (37.5:1/19:1) 450 mM boric acid 1x TBE 10 mM EDTA With sterile H2O ad 9.7 ml pH 8.3 boric acid 300 µl 10 % (w/v) APS 5 µl TEMED - 27 - Material and Methods Washing solution Staining solution 45 % (v/v) Methanol 0.5 % (w/v) Coomassie Brillant Blue 10 % (v/v) Acetic acid 10 % (v/v) Acetic acid Destaining solution 10 % (v/v) Acetic acid 4.4.4 Media and supplements LB-Medium 10 g/l tryptone 5 g/l yeast extract 10 g/l NaCl Additionally 1.5 % (w/v) agar for solid medium Antibiotics Antibiotics were prepared as 1000x stock solutions. Ampicillin and Kanamycin were solved in Millipore, Chloramphenicol was solved in 70 % (v/v) Ethanol. After filtration with a 0.2 µM filter, the solutions were stored at -20°C. Freshly autoclaved media were cooled down to 50°C and afterwards the antibiotics were added to their final concentrations: Antibiotic Ampicillin Kanamycin Chloramphenicol Final concentration 100 µg/ml 100 µg/ml 25 µg/ml Application E. coli E. coli E. coli IPTG IPTG was prepared at 1000x stock solution and added to the medium to a final concentration at 1 mM. 28 Material and Methods 4.5 Methods 4.5.1 General methods Table 4.9: General methods Method Determination of absorbtion of DNA solutions Ethidium bromide staining of DNA Gelelectrophoresis of DNA Ligation of DNA fragments Plasmid preparation from E. coli DNA precipitation DNA sequencing Determination of protein concentrations Denaturating gel electrophoresis of proteins Preparation of CaCl2 competent E. coli cells Reference (Sambrook, 2012) (Sambrook, 2012) (Sambrook, 2012) (Sambrook, 2012) (Holmes & Quigley, 1981) (Sambrook, 2012) (Sanger et al., 1977) (Bradford, 1976) (Laemmli, 1970) (Ausubel, 1987) 4.5.2 Growth of bacteria If not stated differently, bacteria were grown in shaking flasks or glasses filled with the chosen medium at 37°C by continuous shaking at 180-200 rpm till the desired OD600 was reached. Incubation of overnight cultures longed for 16-18 hours. 4.5.3 Transformation of E. coli 4.5.3.1 Preparation of CaCl2 competent E. coli cells 50 ml LB were inoculated with 500 µl of an overnight culture and grown at 37°C by continuously shaking. Growth was stopped at an OD600 of 0.6 by incubation of the cells on ice for 10min. The cells were harvested by centrifugation at 4000 x g for 10min at 4°C. After removal of the supernatant the cell pellet was resuspended in 25 ml (half volume of the culture) cold 0.1 M CaCl2 solution, followed by a second incubation step for 20min on ice. The cells were centrifuged once more at 4000 x g for 10min. The cells were resuspended in 5 ml ice-cold 0.1 M CaCl2 solution (1/10 culture volume) and adjusted to 15 % (v/v) glycerol. Before utilization the cells were either incubated for at least one hour on ice or aliquoted at 500 µl, quick frozen with liquid nitrogen and stored at -80°C. - 29 - Material and Methods 4.5.3.2 Transformation of E. coli The competent cells were carefully thawed on ice and aliquots of 100 µl were added to 10-100 ng DNA. After 20min incubation on ice the heatshock was carried out at 42°C for 90s followed by a second incubation on ice for 10min. 1 ml LB medium was added and the mixture was shaken for at least one hour at 37°C. Finally, the cells were spread on selective media and incubated over night at 37°C. 4.5.3.3 Long time storage of bacterial strains 600 µl of an overnight culture were mixed thoroughly with 300 µl 60 % (v/v) glycerol and frozen immediately in liquid nitrogen. The cells were stored at -80°C. 4.5.4 Methods for purification and modification of nucleic acids 4.5.4.1 Hybridization of cre DNA Equal amounts of complementary strands were mixed in PCR tubes and hybridized in a PCR program by a first melting step at 98°C followed by heating and cooling the sample around the melting temperature (Tm) several times. Finally, the samples were cooled down to room temperature and stored after the hybridization at 4°C. The detailed program is shown below. The hybridized DNA samples were used without further preparation steps. 1x 15x 1x 98°C melting Tm-5°C Tm hybridization Tm+5°C Stepwise cooling down to room temperature 5min 30s 30s 30s 3min/step All 26 nt oligonucleotides were purchased at MWG Eurofins/Ebersberg, the forward strand was biotinylated at the 5’ end. - 30 - Material and Methods 4.5.4.2 Isolation of chromosomal DNA from B. subtilis A 4 ml overnight culture was harvested by centrifugation at 13000 x g /4°C, resuspended in 350 µl lysis buffer and this mixture was incubated at 37°C for 120min. Lysis buffer 100 mg lysozyme 100 μl 1 M Tris/HCl pH 8.0 20 μl 0.5 M EDTA pH 8.0 Ad 2.5 ml H2O 5 µl RNAseA (100 mg/ml) were added, the assays incubated for 2min and afterwards 50 µl Proteinase K (20 mg/ml) were added. The following steps were carried out with the buffers of the QIAamp® DNA Mini Kit from Qiagen. 400 µl ATL buffer were added to the samples and then incubated at 70°C for 30min. After an addition of 400 µl AL buffer the cell debris were precipitated by mixing the sample with 700 µl ice cold 100 % (v/v) ethanol, subsequently incubated at room temperature for 5min. To separate the cell debris from the DNA the samples were centrifuged at 11000 x g /4°C for 10min. The supernatant was loaded to a column by centrifugation at 8000 x g /4°C for 1min. The samples were washed twice with buffer AW and the chromosomal DNA was eluted in two steps with AE buffer prewarmed to 70°C. The chromosomal DNA was stored at 4°C in the fridge. 4.5.4.3 Isolation of plasmid DNA from E. coli Plasmid purifications of 100 ml and 500 ml overnight cultures were carried out with the NucleoBond® AX100 and AX500 kit , for 4 ml overnight cultures the NucleoSpin® Plasmid kit was used according to the manufacturer’s manual (Macherey-Nagel/Düren). The final DNA concentration was determined either by usage of the Nanodrop™ spectrophotometer (Peqlab, Erlangen) or by estimation from a 1 % agarose gel. - 31 - Material and Methods 4.5.4.4 Polymerase chain reaction (PCR) For polymerase chain reactions either chromosomal or plasmid DNA was used as template. In general the mixtures were composed as follows: template DNA 5’ and 3’ oligonucleotide HF buffer dNTP mixture* Taq or Phusion polymerase H2O 0.1-1 µg 10 pmol 1x 0.5 mM 2 Units Ad 20-100 µl * dNTP mixture contained 10 mM dATP, 10 mM dTTP, 10 mM dCTP, 10 mM dGTP General PCR setup: 1x 25-30x 1x 95-98°C (First initial denaturation) 95-98°C (Denaturation) 55-65°C (Annealing) 72°C (Polymerisation) 72°C 1min 30sec 30sec 2min 7min After the reaction the samples were cooled down to 4°C. Annealing temperatures were calculated by Clone Manager™ (Version 9) according to the hybridization temperatures. 4.5.4.5 Restriction of DNA Restriction of DNA was carried out in the buffers and under the conditions recommended by the manufacturer. The amount of DNA determined the amount of enzyme and the incubation time. If necessary, enzymes were inactivated according to the manufacturer’s manual. The results were checked by loading the samples on 1 % agarose gels. - 32 - Material and Methods 4.5.4.6 Dephosphorylation of DNA To dephosphorylate the vector after restriction, the enzymes were inactivated by freezing the samples for 20min at -20°C. Afterwards 1 U calf intestine phosphatase (CIP) was used per 50 ng DNA in 1x CIP buffer (New England Biolabs, Bad Schwalbach) and the samples were incubated at 37°C for one hour. The reaction was stopped by freezing the samples again at least for 30min at -20°C. 4.5.4.7 Ligation of DNA fragments DNA fragments were ligated with the T4 DNA Quickligase™ in the recommended buffer. Inserts were used in 2- to 5-fold excess in contrast to the vector. The samples were incubated at room temperature for a maximum of 20min in the dark. 4.5.4.8 Elution of DNA from agarose gels DNA fragments were separated by electrophoresis with 1 % agarose gels. These were stained in ethidiumbromide for 5-10min and subsequently washed in H2O for 5-10min. The desired DNA bands were cut out the gel using UV light and purified by usage of the NucleoSpin® Gel and PCR clean up kit according to the manufacturer’s manual. The purification was checked on an agarose gel afterwards. 4.5.4.9 Sequencing of DNA Sequencing of DNA was carried out by GATC Biotech AG (Konstanz). Samples were treated like recommended by the company. - 33 - Material and Methods 4.5.5 Methods for purification and analysis of proteins 4.5.5.1 Denaturating protein gel electrophoresis (PAGE) 10 % PAA gels with a width of 1 mm were prepared like introduced by Laemmli (Laemmli, 1970). All samples were mixed with loading dye, denaturated at 90°C for 5-10min and loaded. Afterwards the gels were run at 80-200 V in a Mini-PROTEAN® Tetra Cell chamber (BioRad, Munich). After electrophoresis the gels were fixed for 20min in washing solution, stained for 5-20min in staining solution and destained in destaining solution until the optimal intensity of bands was reached (compare to 4.4.3.2.) 4.5.5.2 Native PAGE Native protein gels were prepared like stated in chapter 4.4.3.2. 15 µl samples were mixed with 5 µl native loading dye and loaded directly to the gel. The gels were run at 80-100 V, the staining and destaining procedure was identical to the SDS gels (compare to 4.5.5.1). 4.5.5.3 Cell cultivation for protein overexpression 1 l LB medium with the corresponding antibiotics was inoculated with a fresh 4 ml overnight culture of the relevant strain. The cells were cultivated at 37°C and the OD600 was monitored until 0.4-0.6. At this point the overexpression was induced by addition of 1 mM IPTG (final concentration). The cells were grown for further three hours. Afterwards the culture was harvested at 5000 rpm/4°C for 10min and pellet stored at -20°C. Before induction and three hours after induction, 5OD equivalents were taken as controls and treated like the main pellet. 4.5.5.4 Sonication The cell pellets were resuspended in 30 ml chilled HisA buffer or Buffer A, respectively, the controls were adjusted to 20 OD/ml and resuspended in HisA buffer/ Buffer A as well. The cells were ruptured by the Labsonic Ultra sonifier, samples up to 2 ml were ruptured with the small, bigger samples with - 34 - Material and Methods the big sonifier. 3-5 pulses at 30s at 45 W were carried out with permanent cooling of the samples, breaks in between the pulses lasted at least 30s, while the sample was kept on ice all the time. 4.5.5.5 Overexpression test The overexpression of the proteins Mdh(His)6, Icd(His)6, IcdS104P(His)6 and CitZ(His)6 was examined by growing and inducing the relevant strains like described in chapters 4.5.5.3 and 4.5.5.4. Before induction, 90min and 180min after induction 5 OD equivalents were taken, harvested by centrifugation at 5000 x g/4°C for 10min and the pellets were stored at -20°C. The cells were adjusted at 20 OD/ml with HisA buffer, ruptured by sonication and the supernatant was removed. To discover misfolded proteins in inclusion bodies the pellet with the cell debris was treated as follows. The pellets were resupended again in the same volume of HisA buffer with 2 % Triton X 100, incubated on ice for 30min and centrifuged for 20min at 13000 x g /4°C. The supernatant was removed and the pellets were washed twice with SBT buffer. Finally the pellets were resuspended in HisA buffer containing 8 M Urea to finally solve the pellet. The samples were warmed at 37°C, mixed with SDS PAGE loading dye and 0.25 OD equivalents of supernatant and pellet fraction were analyzed on an SDS PAGE. 4.5.5.6 Purification of CcpA(His)6, Mdh(His)6, Icd(His)6, IcdS104P(His)6 and CitZ(His)6 from Bacillus subtilis To purify CcpA(His)6, Mdh(His)6, Icd(His)6, IcdS104P(His)6 and CitZ(His)6 E. coli BL21(DE3)/pLysS or E. coli FT1/pLysS cells with the corresponding plasmids pWH653, pGP385, pWH2410, pGP389 and pWH2421 were grown like described above (see points 4.5.5.3 and 4.5.5.4). As overexpression of citZ(His)6 at 37°C lead to formation of inclusion bodies, the overexpression was carried out at 20°C after the induction with IPTG and the cells were shaken over night for approximately 18h. The pellets were resuspended in 30 ml HisA buffer with 1x Complete® protease inhibitor (Roche) and ruptured by sonication. Afterwards 30 µl 10 mg/ml DNAse I and 30 µl 5 mg/ml RNAseA were added to the sample and the mixtures were kept on ice for at least 20min. Subsequently, the cell debris were removed by centrifugation at 20000 rpm/4°C for at least 45min and the supernatant was decanted immediately afterwards. - 35 - Material and Methods The purification was carried out with an Äkta Prime™ chromatography system, as first purification step the proteins were purified by a 5 ml NiNTA column. After a washing step with 15 column volumes (CV) of HisA buffer the protein was eluted with a linear gradient of 15 %, 30 %, 50 %, 70 % and finally 100 % HisB buffer. Protein containing fractions were analyzed by SDS-PAGE and merged afterwards. To reduce the volume to 5 ml the protein solution was condensed via centrifugation with Centriprep filters (MWCO 10 or 30 kDa) at 1500 x g/4°C. The condensed sample was loaded on the Superdex G75 size exclusion chromatography column for further purification and transfer to the HBSE storage buffer. Pure protein fractions were further concentrated and stored in HBS-E buffer at 4°C for approximately 4 weeks or in HBS-E with 50 %(v/v) glycerol at-20°C. 4.5.5.7 Preparation of E. coli DH5α p4813/ HPr kinase extract E. coli DH5α p4813 cells were grown in 100 ml LB medium to an OD600 of 2.0-2.5 and harvested by centrifugation at 5000 rpm/4°C for 5min. The pellet was washed in 40 ml DTT washing buffer, centrifuged once more and resolved in 10 ml DTT lysis buffer. After sonication the cell extracts were mixed with glycerol (final concentration: 10 % (v/v)), aliquoted at 800 µl and stored at -20°C. DTT washing buffer DTT lysis buffer 20 mM Tris/HCl pH 7.6 0.2 mM Tris/HCl pH 7.6 3 mM DTT 0.03 mM DTT 0.5 mM PMSF 4.5.5.8 Purification of HPrSer46P For purification of HPrSer46P, E. coli FT1/pLysS/pWH466 was grown and induced like described above. The cell pellets were solved in 30 ml Buffer A containing 1x Complete® protease inhibitor (Roche) and ruptured (compare to chapter 4.5.5.4). The cell debris were removed by centrifugation at 20000 rpm/4°C for at least 30min. 30 µl 10 mg/ml DNAse I and 30 µl 5 mg/ml RNAse A were added to the supernatant and the samples were incubated on ice afterwards for 20min. To remove heat instable proteins, the samples were heat up to 70°C for 20min and subsequently centrifuged once more at 20000 rpm/4°C for at least 30min. The supernatant was decanted immediately afterwards. - 36 - Material and Methods HPr was phosphorylated at Serine 46 by usage of the HPr kinase extract (compare to chapter 4.5.5.7). The components listed below were mixed thoroughly and incubated for 20min at 37°C. HPr crude lysate: 500 mM ATP 1 M FBP 10x R-Mix Kinase extract H2O 25-29 ml 340 µl 340 µl 3.4 ml 800 µl Ad 35 ml 10x R-Mix 1 M Tris/HCl pH 7.5 1 M MgCl2 1 M DTT H2O 2 ml 500 µl 100 µl Ad 10 ml The complete phosphorylation assay was loaded onto the QFF sepharose column. After a washing step with Buffer A the proteins were eluted by a linear gradient (50 ml, 0-50 % Buffer B) followed by a final elution step with 30 ml of 100 % Buffer B. Fractions containing HPrSer46P were condensed to a final volume of 5 ml and finally transferred to the storage buffer HBS-E by size exclusion chromatography with a Superdex G75 column. HPrSer46P was condensed to the final concentration and stored in the fridge for approximately 4 weeks. 4.5.5.9 Dialysis of protein solutions As glycerol disturbs surface plasmon resonance measurements, it was removed via dialysis in SlydeA-Lyzer dialysis cups with a maximal volume of 100 µl against a 1000-fold volume of HBS-E buffer at 4°C by slight stirring. Buffers were exchanged after one hour and two hours followed by al final dialysis overnight. 4.5.5.10 Surface plasmon resonance (SPR) analysis All SPR measurements were carried out on a BiacoreX instrument at 25°C. For all kinds of measurements the carboxylated dextran matrix of the CM5 chips was activated by injection of a 35 µl mixture of 50 mM N-hydroxysuccinimide (NHS) and 200 mM N-ethyl-N’-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) solved in H2O. After coupling of the relevant ligand the remaining activated carboxyl groups were deactivated by injection of 35 µl 1 M ethanolamine hydrochlorideNaOH pH 8.5. HBS-EP purchased by Biacore (GE Healthcare, Munich) served as running buffer for all kinds of measurements. The optimal pH for coupling of ligand proteins was examined by the - 37 - Material and Methods “immobilization pH scouting protocol” by Biacore/GE Healthcare and was based on the natural isolelectric point of each protein. The different assays were realized as follows: CcpA(His)6 – HPrSer46P: 500 nM CcpA(His)6 and as reference TetR(BD) from E.coli were solved in 10 mM sodium acetate pH 5. Around 3000 RU of CcpA(His)6 was coupled in flowcell (FC) 2, the same amount of TetR(BD) was coupled in the reference cell FC1 at a flowrate of 5 µl/min. As high amounts of CcpA(His)6 dissociated from the chip after coupling, the chip bleeded overnight, until the amount of CcpA(His)6 on the chip was stable. The amount of HPrSer46P of each sample was adjusted with HBS-EP buffer and sensorgrams were recorded at a constant flowrate of 20 µl/min as stated before (Seidel, 2005; Seidel et al., 2005). CcpA(His)6 – HPrSer46P – cre-sites: To analyze the binding of the CcpA-HPrSer46P complex to various cre-elements on the activated chip approximately 4000 RU of Neutravidin were coupled in each of the flowcells. After deactivation with ethanolamine 100 RU of hybridized 26 nt cre DNA biotinylated at the 5’ end was coupled to FC2 and the same amount of 26 nt reference DNA to FC1. For qualitative analysis the DNA on the chip was titrated with 10 nM CcpA(His)6 and varying amounts HPrSer46P (1100 µM). Quantitative measurements were carried out with samples containing varying amounts of CcpA(His)6 (2-100 nM) and 50 µM HPrSer46P adjusted with HBS-EP buffer. To analyze the binding of the whole complex to the cre-element the running buffer had to be supplemented with 50 µM HPrSer46P, in the case of gntR-credown with 75 µM HPrSer46P to saturate CcpA fully with HPrSer46P. Minimal masstransport was revealed at a flowrate of 40 µl/min and therefore it was chosen for all measurements (Schumacher et al., 2011; Seidel, 2005). To induce the dissociation of the complex 80 µl pure HBS-EP buffer were injected. All kinetic parameters were calculated by BIAEVALUATION software 4.1 from the given sensorgrams using the 1:1 Langmuir binding fit. All kinetic data were obtained from three independent measurements. Mdh(His)6 –Icd(His)6/IcdS104P(His)6/CitZ(His)6: Mdh(His)6 was diluted to 500 nM in 10 mM sodium acetate pH 4 and 3500 RU were coupled to FC2. As reference cell served the empty FC1, which was only activated and deactivated immediately afterwards. Icd(His)6/IcdS104P(His)6 or CitZ(His)6 samples were adjusted to the correct amount (1-100 µM) by diluting with HBS-EP buffer and titrated to Mdh(His)6. Since no masstransport was detectable at a flowrate of 20 µl/min, this was chosen for all measurements. To remove Icd and regenerate the chip after each cycle, 50 µl 0.05 % (v/v) SDS dissolved in HBS-EP buffer were injected. For measurements with different metabolites and cofactors, all samples and the HBS-EP running buffer were supplemented with the same amount of metabolites, NADP(H), NAD(H) and MgCl2 from stock solutions to reduce bulk effects. The pH in all stock solutions was adjusted to 7.4 with 5 % (v/v) NaOH. Samples containing mixtures of enzyme, metabolites and cofactors were injected immediately after mixing of the components. Association - 38 - Material and Methods and dissociation rate constants (ka and kd) and the resulting dissociation constant KD were calculated from the sensorgrams using the 1:1 Langmuir binding fit from the BIAEVALUATION software 4.1. All kinetic data were obtained from three independent measurements. 4.5.5.11 Icd activity assay The activity of Icd was determined according to the protocol of Ellis & Goldberg (Ellis & Goldberg, 1971) with tripiclates and at least two biological replicates. Each sample contained the following components which were all dissolved in 0.1 M triethanolamine pH 7.3: MgCl2 NADP+ Icd/IcdS104P 100 mM triethanolamine pH 7.3 1.7 mM 440 µM 1-2 µg/200 µg Ad 900 µl This mixture was pre-warmed at 37°C for 15min. To ensure a constant temperature of 37°C during the whole measurement the Ultrospec photometer was placed in the 37°C incubator room. The reaction was started by addition of 6.7 mM isocitrate and the increase in A340nm caused by the formation of NADPH was followed for 5mins. A sample containing all components except the enzyme served as blank control and was handled like the enzyme containing samples. The obtained values were plotted against the time and the slope of the linear part of the curve was used to calculate the enzyme turnover/min. The activity of Icd per milligram protein was calculated with the following formula (compare to (Bisswanger, 2004) =U/mg εmM(NADPH): 6.3 mM-1 cm-1, L (length of the cuvette): 1 cm, - 39 - Material and Methods 4.5.5.12 Mdh activity assay To determine the activity of Mdh the protocol of Luo (Luo et al., 2006) was used. All measurements were carried out at 37°C in the incubator room. One sample contained the following components: Rotenon BSA PMS (Phenazine methosulfate) INT (p-Iodonitrotetrazolium-Violet) NAD+ Mdh 0.1 M KH2PO4/K2HPO4 buffer pH 7.8 3 µM 1 mg/ml 0.2 mM 0.6 mM 0.5 mM 100 ng Ad 925 µl Stock solutions of BSA, PMS and INT were dissolved in Millipore water, Rotenon in 100 %(v/v) ethanol (Kuznetsov et al., 2008) and NAD+ and malate in 0.1 M KH2PO4/K2HPO4 buffer pH 7.8. Except of BSA and Rotenon, all components were always prepared freshly before use. As PMS is highly light sensitive, the samples were protected against light all the time. After thoroughly mixing the components were incubated for exactly 10min at 37°C and the measurement started immediately afterwards by addition of 15 mM malate. As blank control a sample containing everything except the enzyme was measured as well. The increase in A500nm was followed for three minutes. A500nm was plotted against the time and according to the slope of the linear part of the curve the turnover/min was calculated. =U/mg εmM cm(INT): 12.4 mM-1 cm-1, L (length of the cuvette): 1 cm All measurements were carried out with triplicates with at least two biological replicates. 4.5.5.13 CitZ activity assay The activity of CitZ was determined with the commercial “Citrate Synthase Assay Kit” from SigmaAldrich according to the manufacturers’ manual. The analyzed samples contained 1-10 µg purified CitZ(His)6. - 40 - Material and Methods 4.5.6 Computer programs, databases and online services The computer programs, databases and online services used during this work are listed in the two tables below. 4.5.6.1 Computer programs Table 1.1: Computer programs Program Adobe® Photoshop® CS4 BIACORE X Software 2.2 BIAEVALUTION software 4.0 ChemBioDraw Ultra Version 12.0.2.1076 Clone manager 9 professional edition DNASTAR SeqMan™ II Endnote X.4.0.2 Microsoft® Office Home & Student 2010 Sigma Plot 9.0 Swiss Pdb viewer Version 4.0.4 Vector NTI Suite 8.0 Application Image processing SPR analysis Evaluation and processing of SPR data, calculation of kinetic constants Drawing of chemical formulas Processing of DNA sequence data, primer design and administration of primer and vector database Sequence alignment editor (ABI-files) Generation of literature database Generation of text documents, tables, diagrams and presentations Generation of scientific diagrams Analysis of protein structures Processing of DNA sequence data, primer design and administration of primer and vector database 4.5.6.2 Databases and online services Table 1.2: Online services and databases URL http://brenda-enzymes.org http://dbtbs.hgc.jp/ http://genodb.pasteur.fr http://rzblx1.uniregensburg.de/ezeit/ http://string.embl.de/ Provider Technical university of Braunschweig Human Genome Center, Inst. Med. Sci., Univ. Tokyo; Institute Pasteur, Paris, France University library of Regensburg Centre of protein research, University of Copenhagen, Denmark; Swiss institute of Bioinformatics, Lausanne Switzerland, Biotechnology Center, TU Dresden, University of Zurich, Switzerland - 41 - Application Analysis of enzymes Research in the genome of B. subtilis Genome database of B. subtilis and E. coli Research of literature Analysis of protein interaction networks Material and Methods URL http://www.ncbi.nlm.nih.gov/ Provider National Institutes of Health, USA http://www.rcsb.org/ Rutgers, the State University of New Jersey, San Diego Supercomputer Center (SDSC) and Skaggs School of Pharmacy and Pharmaceutical Sciences, USA The University of Manchester, HITS gGmbH https://seek.sysmo-db.org/ - 42 - Application Research of literature, BLAST server, DNA and protein sequences Research of protein structures Database of the SysMO consortium Results 5 Results 5.1 Metabolite dependent interaction of Isocitrate dehydrogenase with Malate dehydrogenase of Bacillus subtilis The interaction of the enzymes of the TCA cycle enables the cell to transport the metabolites efficiently from one enzyme to the next without time consuming search of the next interaction partner (Barnes & Weitzman, 1986; Mitchell et al., 1995; Ovádi & Srere, 1996). In preliminary studies, the interaction of the malate dehydrogenase Mdh and the isocitrate dehydrogenase Icd were detected for the first time in B. subtilis by SPINE and Bacterial-Two-Hybrid experiments (Meyer et al., 2011). The aim of this project was to elucidate the conditions for these interactions in more detail and see if they are influenced by the educts and products of both enzymes. This was approved by surface plasmon resonance (SPR) with the proteins alone and the corresponding metabolites. In a second step the influence on the activity of both proteins by the interaction partner was analyzed. 5.1.1 Purification of Mdh(His)6, Icd(His)6 and IcdS104P(His)6 To purify B. subtilis Icd with a N-terminal His6-tag, the gene was amplified via PCR from chromosomal B. subtilis DNA with the primers IcdfwBam and IcdrevXho. The PCR product and the overexpression vector pET28b(+) were cut with the restriction enzymes BamHI and XhoI and after a purification step ligated with T4 DNA quickligase. The resulting vector was named pWH2410. The overexpression vectors for Mdh(His)6 (pGP385) and the IcdS104P(His)6-mutant (pGP389) were obtained from Jörg Stülke/Georg August University Göttingen. The genes were amplified via PCR with chromosomal DNA as template, cut with the restriction enzymes BamHI and HindIII and ligated with the vector pWH844 which was ligated with the same enzymes (Pietack et al., 2010). E. coli BL21 (DE3)/pLysS or E. coli FT1/pLysS were used as overexpression strains. The detailed procedure concerning overexpression and purification is described in chapter 4.5.5.6. The first purification step was carried out via Ni2+ affinity chromatography on a NiNTA–column, subsequently - 43 - Results the proteins were transferred via size exclusion chromatography to the HBS-E storage buffer. All three proteins were purified successfully without any impurities. No degradation bands were visible. The picture below (Figure 5.1) shows all three proteins after the purification, Icd(His)6 and IcdS104P(His)6 with 49.7 kDa and Mdh(His)6 with 36.9 kDa. 1 2 3 4 Icd(His)6 : 49.7 kDa Mdh(His)6 : 36.9 kDa Figure 5.1: Purified Icd(His)6, IcdS104P(His)6 and Mdh(His)6. This 10 % SDS-PAGE was loaded with 2 µg of each protein. The first lane contained the PeqGold protein marker II, lane 2 Icd(His)6, lane 3 IcdS104P(His)6 and in lane 4 Mdh(His)6 5.1.2 Interaction of Mdh(His)6 and Icd(His)6 To analyze the interaction of Mdh(His)6 and Icd(His)6 with SPR, 3500 RU of Mdh were coupled to a CM5 chip via amide coupling in flowcell (FC) 2, as reference cell served the empty FC1. Despite of the high amount of coupled Mdh(His)6 the titration of 10-70 µM Icd(His)6 caused only a very weak binding signal which was too low to perform a quantitative analysis of the binding. To test for the specificity of the signal of the Mdh-Icd interaction 1-30 µM of the Tet-Repressor variant TetR(B), a protein not available in the B. subtilis cell under normal conditions, were titrated as well. Hereby no interaction with Mdh(His)6 was detectable (Figure 5.2). Figure 5.2: Interaction analysis of Mdh(His)6with Icd(His)6 or TetR(B). Sensorgrams from titrations of Mdh(His)6 with Icd(His)6 (A)or TetR(B)(B) to Mdh(His)6. The sensorgrams correspond to the given concentrations on the right from the bottom to the top. All sensorgrams were recorded from three biological replicates. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His) 6) and the empty reference cell FC1. - 44 - Results 5.1.3 SPR analysis of the interaction of Icd(His)6 and Mdh(His)6 in the presence of cofactors and substrates To explore the influence of the metabolites on the binding of the two enzymes, their educts and products as well as the reduction equivalents NADP+ or NAD+ and the metal ion cofactor of Icd Mg2+ (Hurley et al., 1991; Singh et al., 2001) were added separately and in combination to the sample. The same amount of each supplement had to be added to the HBS-EP running buffer as well to reduce bulk effects as discovered before in similar experimental setups (Seidel et al., 2005). The chosen amounts of isocitrate, α-ketoglutarate, malate and oxaloacetate as well as NADP+/NADPH and NAD+/NADH correspond to the known intracellular concentrations of B. subtilis or to those of E. coli, respectively (Buescher et al., 2012; Chassagnole et al., 2002; Hurwitz & Rosano, 1967; Markuszewski et al., 2003). 5.1.3.1 SPR analysis of Icd(His)6 binding to Mdh(His)6 in the presence of isocitrate, α-ketoglutarate, malate and oxaloacetate To investigate the influence of the four metabolites, subsequent titrations with 20 µM Icd(His)6 were carried out and the samples contained the following components: Metabolite 1 mM isocitrate (educt of Icd) 1 mM α-ketoglutarate (product of Icd) 1 mM malate (educt of Mdh) 1 mM oxaloacetate (product of Mdh) Cofactor 0.5 mM NADP+ 0.5 mM NADPH 0.5 mM NAD+ 0.5 mM NADH Metal ion cofactor 5 mM MgCl2 5 mM MgCl2 5 mM MgCl2 5 mM MgCl2 As described in chapter 5.1.3 the HBS-EP running buffer had to be supplemented with the corresponding metabolite/cofactor mixture to reduce bulk effects (for more details see 4.5.5.10). All components listed above were injected separately or in double or triple combination together with 20 µM Icd(His)6. The SPR analysis revealed that neither 1 mM isocitrate nor 0.5 mM NADP+ nor 5 mM Mg2+ alone changed the binding of Icd(His)6 to Mdh(His)6. Only the triple combination of 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2 stimulated the binding of Icd(His)6 to Mdh(His)6 and resulted in a 7.5-fold higher signal in an endpoint assay, which is shown in Figure 5.3 A. - 45 - Results The product of Icd, α-ketoglutarate, either alone or together with NADPH and MgCl2, did not alter the binding to Mdh(His)6 (Figure 5.3 B). Neither did malate or oxaloacetate, the educt and product of Mdh, alone or in combination with the appropriate reduction equivalents NAD+ or NADH influence the binding of Icd(His)6 to Mdh(His)6 (Figure 5.3 C/D). Figure 5.3: SPR analysis of Icd(His)6 binding to Mdh(His)6 with different metabolites and cofactors. The diagram shows sensorgrams from subsequent injections of 20 µM Icd(His)6 (light green sensorgram) on a Mdh(His)6 coated CM5-Chip. The running buffer and the samples were supplemented with the metabolite mixtures written on the right side of the sensorgram, the color of the sensorgram corresponds to the color of + the legend. The interaction of the two enzymes is only stimulated by addition of isocitrate, MgCl2 and NADP + (A). Neither the combination of α-ketoglutarate and NADPH (B), nor malate/NAD (C), nor oxaloacetate in combination with NADH did change the binding of Icd(His) 6 to Mdh(His)6. All measurements were reproduced at least two times. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His) 6) and the empty reference cell FC1. - 46 - Results 5.1.3.2 Influence of the enzymatic activity of Icd(His)6 on the binding to Mdh(His)6 If the SPR samples contained besides Icd(His)6 isocitrate, NADP+ and Mg2+ the enzyme was in its active conformation (Singh et al., 2001) and able to convert its substrate isocitrate to αketoglutarate. This affected the sensorgrams, because, as a consequence, the samples contained then a mixture of isocitrate/α-ketoglutarate and NADP+/NADPH. This was in contrast to the HBS-EP running buffer which was supplemented with 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2. The sensorgram of an injection of a mixture of 20 µM Icd(His)6, 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2 in flowcell 1 (red curve, Figure 5.4) resembled more a sensorgram of an injection of 0.1 mM α-ketoglutarate, 50 µM NADPH and 5 mM MgCl2 (black curve, Figure 5.4) than pure HBS-EP buffer supplemented with 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2 (blue curve, Figure 5.4). This was an evidence for the activity of the isocitrate dehydrogenase. Due to this activity of the enzyme the stimulation effect of isocitrate, Mg2+ and NADP+ on the binding at Mdh(His)6 was reduced because α-ketoglutarate and NADPH did not cause any stimulation on the binding (compare to Figure 5.3 B). Therefore, the interaction of Mdh(His)6 and the wildtype protein could not be quantified. Figure 5.4: Effect of the activity of Icd on the sensorgram by injection together with isocitrate, MgCl 2 and + NADP . This diagram shows flowcell 1 of a CM5 Chip and its reaction to the titration of 20 µM Icd(His)6 + (red curve) in comparison to pure HBS-EP running buffer with 1mM isocitrate and 0.5mM NADP (blue curve) and an injection of 0.1mM α-ketoglutarate and 50 µM NADPH (black curve). Due to the activity of the enzyme the sensorgram looks more like the one of the injection of α-ketoglutarate/NADPH then like + the injection of the HBS-EP buffer with 1mM isocitrate and 0.5mM NADP . All measurements were reproduced at least two times. - 47 - Results 5.2 Interaction of Mdh(His)6 and IcdS104P(His)6 In addition to the wildtype protein, the IcdS104P mutant, which carries a proline at position 104 instead of a serine, was analyzed as well. In E. coli the corresponding serine residue 113 is phosphorylated by AceK, the isocitrate dehydrogenase kinase/phosphatase, which regulates the activity of the isocitrate dehydrogenase (LaPorte & Koshland, 1982; LaPorte et al., 1985). In B. subtilis, the phosphorylation of the enzyme was shown in vitro by PrkC. However, if AceK can phosphorylate the enzyme in vivo as well remained unclear so far (Pietack et al., 2010; Singh et al., 2002; Zheng & Jia, 2010). 5.2.1 Activity of Icd(His)6 and IcdS104P(His)6 To determine the activity of Icd and the mutant IcdS104P the formation of NADPH was monitored spectrophotometrically at A340nm for 5min at 37°C as described by Ellis & Goldberg (Ellis & Goldberg, 1971) (Figure 5.5). A sample containing all buffer components without enzyme served as blank control. The absorption values were plotted against the time and from the linear part of the curve the activity was calculated. For Icd(His)6 in four independent measurements an activity of 8.7 ± 2.9 U/mg protein was calculated, resulting from activity tests containing only 21.5 nM protein. To obtain the activity of IcdS104P, 4.3 µM IcdS104P(His)6 were needed for a spectrophotometrically detectable reaction. This resulted in three independent measurements in an activity of 0.05 ± 0.03 U/mg protein. In comparison to the wildtype enzyme the activity of the IcdS104P mutant is reduced 174-fold. As the activity of wildtype Icd was problematic during the SPR analysis with Mdh, this mutant was chosen to further investigate the interaction of the two enzymes. - 48 - Results Figure 5.5: Determination of Icd activity by measuring formation of NADPH. The activity of Icd was measured spectrophotometrically absorption at 340 nM (A340nm), shown at the y-axis, which corresponds to the formation of NADPH. At the x-axis the time is shown in seconds (s). All measurements were carried out with triplicates, the standard deviation is indicated by the black lines above and below the symbols. The black lines indicate the linear regression of the corresponding data. The meanings of the symbols are indicated in the box above the diagram. 5.2.2 SPR analysis of IcdS104P(His)6 binding to Mdh(His)6 To figure out the interaction of IcdS104P(His)6 3500 RU Mdh(His)6 were coupled to the CM5 like stated before (refer to chapters 4.5.5.10 and 5.1.3.1). 10-70 µM IcdS104P(His)6 were titrated subsequently to chip. In comparison to the wildtype enzyme, the binding signal was stronger which enabled the quantification of the reaction. This was carried out with the BIAEVALUATION software version 4.1 as 1:1 Langmuir binding fit. The quantification revealed the following data: an association rate constant (ka) of 2.0 ± 0.2 x 102 M-1 s-1, a dissociation rate constant (kd) of 1.0 ± 0.07 x 10-3 s-1 and a resulting dissociation constant (KD) of 5.0 ± 0.1 µM. The χ²-value which represented the average deviation of the fit from the experimental data was 1.8-4.8. - 49 - Results Figure 5.6: SPR analysis of IcdS104P(His)6 binding to Mdh(His)6. This sensorgram shows a titration of 10-70 µM IcdS104P(His)6 to Mdh(His)6. The association and dissociation rate constant (ka and kd) and the resulting dissociation constant (KD) were calculated as 1:1 Langmuir binding fit with BIAEVALUATION software version 4.1. Dotted lines represent the measurements, the bold black lines the best calculated fits for the association rate constant. The kinetic constants were calculated from measurements with three biological replicates. RU: response units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His)6) and the empty reference cell FC1. 5.2.3 SPR analysis of IcdS104P(His)6 binding to Mdh(His)6 in the presence of isocitrate, α-ketoglutarate, malate and oxaloacetate As the binding of Icd(His)6 wildtype to Mdh(His)6 was stimulated by 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2, the same analysis was done for the IcdS104P mutant. The samples were treated as already described in chapter 5.1.3.1. The triple combination of 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2 stimulated the binding of the IcdS104P mutant to Mdh(His)6 as well. However, 5 mM MgCl2 added to 20 µM IcdS104P(His)6 already caused a doubling of the signal in comparison to the titration with pure 20 µM IcdS104P(His)6 (see Figure 5.7 light blue curve). As already described for the wildtype strain, the combinations of α-ketoglutarate/NADPH, malate/NAD+ and oxaloacetate/NADH added to subsequent titrations of 20 µM IcdS104P(His)6 had no influence on the binding to Mdh(His)6 (Figure 5.7 B, C, D). - 50 - Results Figure 5.7: SPR analysis of IcdS104P(His)6 binding to Mdh(His)6 with different metabolites and cofactors. The diagram shows sensorgrams from subsequent injections of 20 µM IcdS104P(His)6 (red sensorgram) to Mdh(His)6 coupled to a CM5 chip. The running buffer and the samples were supplemented with the metabolite mixtures written on the right side of the diagram, the color of the sensorgram corresponds to the color of the legend. The interaction of the two enzymes is stimulated by addition of 5 mM MgCl2 (light blue sensorgram). The stimulation + increases after addition of isocitrate, MgCl2 and NADP to the sample (A). Neither the combination of α+ ketoglutarate and NADPH (B), nor malate/NAD (C), nor oxaloacetate in combination with NADH did change the binding of Icd(His)6 to Mdh(His)6. All measurements were reproduced at least two times. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His)6) and the empty reference cell FC1. - 51 - Results 5.2.4 Quantification of the interaction between IcdS104P(His)6 and Mdh(His)6 stimulated by isocitrate, NADP+ and MgCl2 The binding of wildtype Icd to Mdh was stimulated by Mg2+, isocitrate and NADP+. However, the quantification of this interaction by SPR was impeded by the enzymatic activity of Icd. Wildtype Icd converted isocitrate and NADP+ to α-ketoglutarate and NADPH which resulted in high bulk effects. IcdS104P(His)6 showed 174-fold reduced enzymatic activity in comparison to the wildtype enzyme and therefore the supplemented amounts of the cofactors in the sample remained almost constant. Therefore, the mutated enzyme was used to quantify the interaction with Mdh(His)6 stimulated by isocitrate, NADP+ and MgCl2 via SPR. The HBS-EP running buffer was supplemented with 1 mM isocitrate, 0.5 mM NADP+ and 5 mM MgCl2. All measurements were carried out at least three times with at least two biological replicates. 0.5 to 20 µM IcdS104P(His)6 were titrated subsequently to Mdh(His)6 coupled to the CM5 chip. The sensorgrams were evaluated by an 1:1 Langmuir binding fit with the BIAEVALUATION software version 4.1 and revealed the following data: an association rate constant (ka) of 1.7 ± 0.7 x 103 M-1 s-1 , a dissociation rate constant (kd) of 2.6 ± 0.6 x 10-4 s-1 and a resulting dissociation constant (KD) of 150 ± 0.3 nM. The average deviation between the fit and experimental data was given as a χ²- value of 2.3-3.0 in three independent measurements. Figure 5.8: Quantification of the interaction between IcdS104P(His)6 and Mdh(His)6 stimulated by + isocitrate, NADP and MgCl2. The diagram shows a titration of 0.520 µM IcdS104P(His)6 to Mdh(His)6 coupled on the CM5 chip. The running buffer and each sample were supplemented with 1 mM + isocitrate, 0.5 mM NADP and 5 mM MgCl2 to reduce bulk effects. Dotted lines represent the subsequent injections of IcdS104P(His)6, bold black lines the 1:1 Langmuir binding fits calculated from BIAEVALUATION, version 4.1. The kinetic data were calculated from measurements with two biological replicates. RU: response units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His)6) and the empty reference cell FC1. - 52 - Results Compared to the interaction without isocitrate, NADP+ and MgCl2, the affinity of IcdS104P mutant to Mdh(His)6 was therefore 33.3-fold higher (refer to Table 5.1). Table 5.1: Comparison of the kinetic data obtained from the titration of IcdS104P(His) 6 to Mdh(His)6 in + presence and absence of isocitrate, NADP and MgCl2. All data were measured with at least three biological replicates, the kinetic parameters were obtained by calculation with BIAEVALUATION, version 4.1 as 1:1 Langmuir binding fit. IcdS104P(His)6 IcdS104P(His)6, 1mM isocitrate, 0.5 mM NADP+, 5 mM MgCl2 Factor (stimulated/not stimulated) ka [M-1 s-1] 2.0 ± 0.2 x 102 kd [s-1] 1.0 ± 0.07 x 10-3 KD [µM] 5.0 ± 0.1 1.7 ± 0.7 x 103 2.6 ± 0.6x10-4 0.15 ± 0.03 8.5 0.26 33.3 5.3 Mutual influence of Icd and Mdh on their enzymatic activity SPR analysis revealed a weak interaction of Mdh with Icd. As their encoding genes mdh and icd are organized in one operon and therefore they are always co-expressed, this weak interaction might result from a complex formation in situ nascendi during co-translation of both enzymes (Jin et al., 1996; Jin & Sonenshein, 1994b). However, the purpose of interaction of Mdh and Icd is not obvious, because these enzymes do not catalyze subsequent reactions in the TCA cycle. Therefore, Mdh and Icd might be parts of a higher organized enzyme complex of TCA cycle proteins to transfer the product of one enzyme directly to the next enzyme. The existence of a so-called metabolon was analyzed already not only for B. subtilis, but also for E. coli and Pseudomonas aeruginosa (Barnes & Weitzman, 1986; Meyer et al., 2011; Mitchell et al., 1995). However, even without a bigger complex of the TCA cycle enzymes, the activities of Mdh and Icd could be influenced by their interaction, as isocitrate and NADP+, the substrates of Icd, lead to a 33-fold stimulation of the binding to Mdh in SPR measurements. First of all, the impact of the interaction on the enzymatic activity of Mdh had to be determined in vitro alone and in combination with Icd and the mutant IcdS104P. Vice versa the activity of wildtype Icd and the IcdS104P mutant should be investigated depending on Mdh. - 53 - Results 5.3.1 Influence of Icd and IcdS104P on Mdh activity The conversion of malate to oxaloacetate is the only endergonic reaction during the citrate cycle with ΔG of +30 kJ/mol (Campel, 2006), and therefore the activity of Mdh was determined using the coloring agent “p-Iodonitrotetrazolium violet” (INT) according to the protocol of Luo (Luo et al., 2006). The advantage of this method is the fast transfer of the electrons of NADH immediately after formation via PMS (phenanzine methosulfate) to INT. This lead to the formation of a red formazan, which was detected spectrophotometrically (refer to Figure 5.9). As PMS is highly light sensitive, the samples were protected against light during the whole experimental process. The reference cuvette contained all components except the enzyme (refer to chapter 4.5.5.12). Figure 5.9: Comparison of blank control Mdh activity assay to sample containing Mdh. This picture shows two cuvettes after an Mdh activity assay according to Luo (Luo et al., 2006). The cuvette on the left is the blank control containing all components except the enzyme. The cuvette on the right contained 3 nM Mdh(His)6 which led to the formation of the red formazan from “p-iodonitrotetrazolium violet”. This was quantified spectophotometrically at A500nm. - Mdh(His)6 + Mdh(His)6 These analyses revealed an enzymatic activity of 98.4 ± 8.2 U/mg protein for pure Mdh(His)6 (Figure 5.10, blue circles), the addition of 43 nM Icd(His)6 decreased the enzymatic activity slighty to 82.3 ± 4.9 U/mg protein (Figure 5.10, dark green boxes). This fits the SPR data, as wildtype Icd revealed only a weak interaction with Mdh without isocitrate and NADP+. However, as PMS would transfer the electrons of either NADPH or NADH to INT, an experimental setup containing isocitrate and NADP+ as substrates of Icd in addition to malate and NAD+ was not possible (Bériault et al., 2005). As SPR analysis yielded a higher affinity of IcdS104P to Mdh than the wildtype protein even without stimulation of isocitrate, Mg2+ and NADP+, mixtures of the Icd mutant with Mdh were analyzed as well. No stimulation effect was detected and 4.3 µM IcdS104P even reduced Mdh activity to 58.0 ± 13.7 U/mg protein. - 54 - Results In summary the activity of Mdh was hardly influenced by wildtype Icd and even reduced by a factor of 1.7 by IcdS104P. This correlated with the SPR data, as neither the binding of Icd nor the binding of IcdS104P to Mdh was stimulated by malate and NAD+. Figure 5.10: Activity test of Mdh(His)6 with or without Icd(His)6 or IcdS104P(His)6 respectively. This diagram shows the activity of Mdh determined by following the formation of the red formazan from INT (piodonitrotetrazolium violet) spectophotometrically at 500 nm for 180s. The legend above the diagram shows the meaning of the symbols. The straight lines represent the linear regression of the corresponding data. The standard deviation of three independent samples is indicated by the lines above and below of each symbol. The activity of Mdh was slightly decreased by addition of Icd, the mutant IcdS104P reduced the activity of Mdh two-fold. - 55 - Results 5.3.2 Influence of Mdh on the enzymatic activity of Icd and IcdS104P The enzymatic activity of Icd was determined by spectrophotometrical detection of formation of NADPH at A340nm at 37°C. Therefore all components except isocitrate were mixed thoroughly and after equilibration for 10min at 37°C, the increase of A340nm was recorded for 5min. Finally the activity of the enzyme was calculated from the slope of the linear part of the curve (refer to chapter 4.5.5.11). Samples containing all components except isocitrate dehydrogenase served as blank control (Figure 5.11 A&B, black crosses). This assay was highly specific for the activity of Icd, because for samples with pure Mdh no formation of NADPH was observed (Figure 5.11 A&B, blue circles). For Icd(His)6 an activity of 8.7 ± 2.9 U/mg protein was determined (Figure 5.11 A, red squares). The addition of 300 nM Mdh(His)6 doubled the enzymatic activity to 17.3 ± 3.3 U/mg protein (Figure 5.11 A, green unfilled diamonds). In contrast, the activity of the IcdS104P mutant was not affected by the addition of Mdh(His)6 (Figure 5.11 B, red filled triangles and stars, green boxes and diamonds). This might be due to reduced flexibility of the active site in the isocitrate binding pocket of the IcdS104P mutant caused by the fixed N-Cα-angle of proline. Moreover, crystal structures of B. subtilis Icd and the highly homologous corresponding E. coli isocitrate dehydrogenase revealed that four amino acids, including serine residue 104 in B. subtilis and the corresponding serine residue 113 in E. coli, anchor isocitrate and NADP+ at the active site of the enzyme via H-bonds. The weaker fixation might initiate a quick release of the substrate even without turnover (Gonçalves et al., 2012; Hurley et al., 1991; Singh et al., 2001). This had probably a higher impact than the only slight stimulation of the activity caused by Mdh. In summary, the mixture of isocitrate, NADP+ and MgCl2 did not only stimulate the binding of Icd to Mdh, but also doubled the activity of wildtype Icd, if Mdh was available. - 56 - Results Figure 5.11: Activity test of Icd(His)6 and IcdS104P(His)6 with or without Mdh(His)6. Two plots of activity test of Icd (A) and IcdS104P (B) are shown. The legend on the right side of each diagram depicts the contents of the proteins of the samples. The activity of wildtype Icd (A) doubles after addition of Mdh(His) 6. This is not the case for the mutant IcdS104P (B). The straight lines represent the linear regression of the corresponding data. As the linear regression of 4.3 µM IcdS104P and 4.3 µM IcdS104P together with 300 nM Mdh was identical, the line representing the linear regression of 4.3 µM IcdS104P was dashed. The standard deviation of three independent samples is indicated by the lines above and below of each symbol. - 57 - Results 5.4 Interaction analysis of Malate dehydrogenase and Citrate synthase Mdh catalyzes the final reaction step of the TCA cycle, the oxidation of malate to oxaloacetate. Interestingly, this reaction is highly endergonic and therefore, it is driven by the subsequent exergonic conversion of oxaloacetate to citrate (Miller & Smith-Magowan, 1990; Ochoa, 1955). This reaction is catalyzed by CitZ. The encoding genes citZ and mdh are always co-expressed, because they are organized in an operon together with icd (Jin et al., 1996; Jin & Sonenshein, 1996). The in vivo interaction of B. subtilis Mdh and CitZ was detected for the first time by SPINE experiments (Meyer et al., 2011) and it was investigated in vitro by SPR measurements in this study. 5.4.1 Purification of CitZ(His)6 To purify CitZ(His)6, the promotorless citZ gene was amplified from chromosomal B. subtilis 168 DNA via PCR with the oligonucleotides citZfwNhe2 and citZrevBam. The PCR product was cloned in the vector pET-28b(+) via the restriction sites NheI and BamHI. The resulting vector was named pWH2421. Overexpression tests revealed formation of CitZ(His)6 inclusion bodies, if the cells were grown at 37°C. To overcome this problem the cells were grown at 37°C to an OD600 of 0.5 and were then shifted to 20°C. The overexpression was induced by addition of 1 mM IPTG and the cells were grown for 18h overnight. CitZ(His)6 was purified via a 5 ml NiNTA column, the second purification step transferred the protein to the HBS-E storage buffer via size exclusion chromatography. The purification was successful and revealed a pure protein with 43.8 kDa without any degradation (see Figure 5.12). 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 43.8 kDa Figure 5.12: Analytical gel electrophoresis (10 % SDS PAGE) of 15 µl samples of His6-tagged CitZ after size exclusion chromatography. Lane 1 contains the PeqGold protein standard, lane 2 the soluble fraction of the pre-induction sample, lane 3 a control sample taken 18h after induction of overexpression. Lanes 7-15 contain pure CitZ(His)6 after NiNTA and size exclusion chromatography. The molecular weight of the protein is 43.8 kDa. - 58 - Results 5.4.2 SPR analysis of CitZ(His)6 binding to Mdh(His)6 To analyze the interaction of CitZ(His)6 and Mdh(His)6, 3000 RU purified Mdh(His)6 were coupled to flowcell 2 of a CM5-chip, the empty flowcell 1 served as reference cell. Rising concentrations of CitZ(His)6 were titrated subsequently to Mdh at a flowrate of 20 µl/min. CitZ(His)6 precipitated at concentrations above 35 µM, therefore it was not possible to analyze interaction with more than 14 µM CitZ(His)6. Three independent measurements revealed no binding of CitZ(His)6 to Mdh(His)6 (see Figure 5.13). Figure 5.13: SPR analysis of Mdh(His)6 and CitZ(His)6. Sensorgrams from titrations of CitZ(His) 6 to Mdh(His)6. The analyzed CitZ(His)6 concentrations are depicted at the right side of the diagram. All measurements were reproduced three times with two biological replicates. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His)6) and the empty reference cell FC1. 5.4.3 SPR-analysis of CitZ(His)6 and Mdh(His)6 interaction in the presence of malate and oxaloacetate To explore the impact of the substrates of Mdh on the Mdh-CitZ interaction, 5-10 µM CitZ(His)6 were titrated to Mdh(His)6 with various oxaloacetate and malate concentrations. The HBS-EP running buffer was supplemented with the same amount of oxaloacetate and malate to reduce bulk effects. Neither the addition of malate, nor the addition of oxaloacetate did stimulate the binding of CitZ to Mdh. A weak SPR signal was detected after the injection of pure NADH. This signal did not change, if mixtures of CitZ(His)6, oxaloacetate and NADH were injected (refer to Figure 5.14). - 59 - Results Figure 5.14: SPR analysis of the interaction between Mdh(His) 6 and CitZ(His)6 with different substrates and cofactors. The diagram shows sensorgrams from subsequent injections of 5-10 µM CitZ(His)6 (black sensorgram) to Mdh(His)6 coupled to a CM5 chip. The running buffer and the samples were supplemented with the depicted oxaloacetate and malate concentrations written on the right side of the diagram, the color of the sensorgram corresponds to the color of the legend. Neither pure oxaloacetate (A), nor oxaloacetate and NADH (B), nor malate did influence the binding of CitZ(His) 6 to Mdh(His)6. All measurements were reproduced two times with two biological replicates. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (Mdh(His) 6) and the empty reference cell FC1. Neither another coupling method of Mdh to the CM5 chip nor the usage of Strep-tagged CitZ instead of His-tagged revealed any binding between the two enzymes. In summary, it was not possible to detect any interaction of these enzymes by SPR so far. - 60 - Results 5.5 Interaction of various cre-elements with the CcpAHPrSer46P complex The central carbon and nitrogen metabolism, e.g. parts of the TCA cycle, carbon catabolite regulation overflow metabolism and amino acid synthesis in B. subtilis is regulated by the global regulator CcpA (Fujita, 2009; Henkin, 1996; Sonenshein, 2007). In complex with its coeffector HPrSer46P it is able to recognize its binding sites, catabolite responsive elements (cre), 14bp long sequences with a highly degenerate sequence and only very small conserved parts, e.g. the central CG pair (Fujita, 2009; Hueck et al., 1994; Marciniak et al., 2012; Miwa et al., 2000; Weickert & Chambliss, 1990). In this study, the binding of the CcpA-HPrSer46P complex to various cre-elements was analyzed and quantified in vitro via SPR to see if these highly degenerate sequences are bound with different affinities as this could represent an additional regulatory mechanism. This project was accompanied by the crystallization of CcpA-HPrSer46P in complex with different cre-elements (Schumacher et al., 2011). 5.5.1 Purification of CcpA(His)6 and HPrSer46P 5.5.1.1 Purification of CcpA(His)6 To purify CcpA(His)6, E. coli FT1/pLys cells containing pWH653 were grown, induced and harvested like described earlier. The protein was purified in two steps: The first step was carried out on a 5 ml NiNTA column to purify via the Histag. CcpA(His)6 containing fractions were condensed to 5 ml and the protein was purified once again via size exclusion chromatography with a G200 column. This transferred the protein to the HBS-E storage buffer. After condensing the protein to the desired concentration, it was either stored at 4°C for up to four weeks or adjusted to 50 % (v/v) glycerol for long time storage at -20°C. Purified CcpA(His)6 was analyzed via SDS-PAGE (Figure 5.15 A). - 61 - Results 5.5.1.2 Purification of HPrSer46P Purification of HPrSer46P started with the overexpression of E. coli FT1/pLys pWH466 (ptsH). After rupturing the cells by sonication and removal of the cell debris, as a first purification step the heat instable proteins were removed by heating the samples to 70°C for 20min. HPr was phosphorylated at serine residue 46 in vitro by the HPr kinase cell extract and the protein was purified via anion exchange chromatography on a QFF-sepharose column. HPrSer46P containing fractions were collected and condensed to a maximal volume of 5 ml and further purified via size exclusion chromatography with a G75 column to transfer the protein to the HBS-E storage buffer. Purified HPrSer46P was loaded on a 10 % native PAA gel in comparison to unphosphorylated HPr. HPrSer46 was running farther as HPr in the gel as the negative charge is higher (see Figure 5.15 B). Purified HPrSer46P was condensed to the desired concentration and then stored at 4°C for approximately 8 weeks (refer to chapter 4.5.5.8). Figure 5.15: Purified CcpA(His)6 and HPrSer46P. (A) 10 % SDS PAGE of CcpA(His)6, 0.5 and 1 µg were loaded in lane 2 and 3. As control the Peqlab protein standard II is loaded in lane 1. (B) To investigate the success of the in vitro phosphorylation at serine residue 46, HPrSer46P was loaded on a 10 % native polyacrylamide gel. As control served unphosphorylated HPr in lane 1, lanes 2-4 contain 20, 2 and 1 µg of HPrSer46P which is running farther in the native PAGE due to the higher negative charge of the protein. - 62 - Results 5.5.2 Qualitative and quantitative analysis of CcpA coeffector complexes interacting with various cre-elements 5.5.2.1 Selection and preparation of the analyzed cre-elements The analyzed cre-elements should represent a broad range of genes whose expression is typically activated or repressed via CcpA. The cre2 element of ackA was chosen because the expression of ackA is activated by CcpA during exponential growth (Grundy et al., 1993; Turinsky et al., 1998; Wünsche, 2012). Representatives of genes, whose transcription is repressed by CcpA, are xynP, xylA and gntR. XynP and xylA encode proteins for uptake and utilization of xylose and their transcription is only regulated by one cre-element (Galinier et al., 1999; Kraus et al., 1994). However, gntR encoding the repressor of the gntRKPZ operon, has besides the selected gntR-credown an additional HPrSer46P independent cre-site (Fujita et al., 1995; Miwa et al., 1997). The synthetic cre-element syn-cre was created for the first crystallization experiment of Bacillus megaterium CcpA and served as a linker between B. megaterium and B. subtilis in crystallization and SPR (Schumacher et al., 2004; Schumacher et al., 2011). The sequences of all selected cre-elements are shown in Table 5.2. Table 5.2: Sequence of the cre-elements chosen for the binding analysis of the CcpA-HPrSer46P complex. The highlighted red letters represent the parts of the consensus sequences (Hueck et al., 1994; Miwa & Fujita, 2001; Miwa et al., 2000; Weickert & Chambliss, 1989). Syn-cre is short for synthetic cre-site which enabled the first crystallization of CcpA/HPrSer46P of Bacillus megaterium in complex with a cre-element (Schumacher et al., 2004). cre- element ackA-cre2 gntR-credown syn-cre xylA-cre xynP-cre Sequence T T C T T T T T T T G G G G G T A T G A A A T A A A A A A A G G G G G C C C C C G G G G G T G C C C T T T A T A A T A T T C T A T C C C C T A A A G A A The complementary strands of the selected DNA fragments were ordered as separate forward and backward oligonucleotides. Equal amounts of both oligonucleotides were mixed and hybridized via heating up and carefully cooling down around the melting point several times in a PCR thermo cycler (for more details refer to chapter 4.5.4.1). The success of the hybridization finally was checked on a 10 % polyacrylamide gel in comparison to the single-stranded non hybridized DNA oligonucleotides (refer to Figure 5.16). - 63 - Results 1 2 3 4 26 bps Figure 5.16: Hybridization of syn-cre. The 26nt long oligonucleotides were analyzed on a 10 % polyacrylamide gel. Hereby as controls besides the Peqlab ultra low range DNA standard in lane 1, the single stranded forward and backward oligonucleotides were loaded in lanes 2 and 3. The successfully hybridized samples are shown in lanes 4 and 5. 5.5.2.2 Qualitative analysis of CcpA-HPrSer46P binding to xylA-cre To analyze the binding of the CcpA-HPrSer46P heterotetramer to xylA-cre via SPR, approximately 4000 RU Neutravidin – a streptavidin analogue – were coupled on an activated CM5 Chip in both flowcells. As the DNA of the forward oligonucleotide was biotinylated at the 5’ end, it was now possible to couple the DNA to the Neutravidin-coated chip surface. 100 RU of xylA-cre DNA was coupled in FC2, as reference served an unspecific 26nt DNA coupled in FC1 (Horstmann, 2006; Horstmann et al., 2007; Schumacher et al., 2011). Flowrate tests revealed a no mass transport at a flowrate of 40 µl/min, hence this flowrate was used for all measurements. Titrations of 10 nM CcpA(His)6 and 1-75 µM HPrSer46P to xylA-cre revealed that rising concentrations of HPrSer46P stimulated the binding of CcpA(His)6 to the cre-element. Saturation was reached at 50 µM HPrSer46P like seen before for xylA-cre of B. megaterium (Seidel et al., 2005) (refer to Figure 5.17). - 64 - Results Figure 5.17: Association of the CcpA(His)6/xylA-cre complex depending on the HPrSer46P concentration. This diagram shows a titration of 10nM CcpA(His)6 and rising HPrSer46P concentrations to xylA cre. The HPrSer46P concentrations are written on the right side of the diagram. All measurements were reproduced at least two times. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (xylA-cre) and an unspecific oligonucleotide coupled to the reference cell FC1. 5.5.2.3 Quantification of the CcpA HPrSer46P xylA-cre interaction The dissociation of the CcpA-HPrSer46P-cre complex is very fast due to the fast dissociation of the CcpA-HPrSer46P complex. Therefore, the quantification of the interaction of the whole complex with the cre was only possible, if CcpA was saturated with HPrSer46P all the time (Seidel et al., 2005). As xylA-cre was saturated at 50 µM HPrSer46P, this amount was added to each sample and to the HBSEP running buffer as well. Subsequently, rising amounts of CcpA(His)6 were titrated to the creelement, afterwards the dissociation of the CcpA-HPrSer46P complex was induced by injection of pure HBS-EP buffer. The obtained sensorgrams were evaluated and fitted by the BIAEVALUATION software version 4.1 with the 1:1 Langmuir binding model with data from three independent measurements. It was assumed, that one CcpA-HPrSer46P complex binds to one cre-element. The quantification revealed an association rate constant (ka) of 1.1 ± 0.3 x 106 M-1s-1, a dissociation rate constant (kd) of 3.6 ± 1.5 x 10-3s-1 and a resulting dissociation constant KD of 3.3 ± 0.5 nM. The average deviation of the calculated curves from the real experimental sensorgrams was given by the χ²-value of 0.5 – 2.1 (see Figure 5.18). - 65 - Results Figure 5.18: Quantification of CcpA-HPrSer46P complexes interacting with xylA-cre. The two diagrams show sensorgrams of titrations with 1-40 nM CcpA(His)6 and 50 µM HPrSer46P to xylA-cre. The HBS-EP running buffer was supplemented with 50 µM HPrSer46P as well. Dashed lines represent the experimental data, bold black lines the calculated 1:1 Langmuir binding fits. Association rate constant (A) and dissociation rate constant (B) were calculated separately from the same sensorgram. The measurements were reproduced with two biological replicates. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (xylA-cre) and an unspecific oligonucleotide coupled to the reference cell FC1. 5.5.2.4 Quantification of CcpA HPrSer46P complexes interacting with xynP-cre, ackA-cre2, gntR-credown and syn-cre The interaction of the CcpA HPrSer46P complex with xynP-cre, ackA-cre2, gntR-credown and syn-cre was analyzed like described in the previous chapter. The qualitative measurement with 10 nM CcpA(His)6 and titrations of 1-100 µM HPrSer46P were used to determine the HPrSer46P amount that saturated the cre-element. The running buffer and every sample were then supplemented with the determined amount of HPrSer46P, which were 50 µM HPrSer46P for all cre-elements except gntRcredown. For this cre-element the saturating concentration of HPrSer46P was determined at 75°µM. The rate constants were obtained from titrations of 1-100 nM CcpA(His)6 to the cre-elements coupled on the CM5-chip. AckA-cre2, the synthetic cre and gntR-credown were saturated with 100 nM CcpA(His)6, in contrast, only 30 nM CcpA(His)6 were sufficient to saturate xynP-cre (see Figure 5.19). - 66 - Results Figure 5.19: Kinetic analysis of CcpA-HPrSer46P interaction with various cre-elements via SPR. These diagrams show titrations of 1-100 nM of CcpA(His)6 to ackA-cre2 (A), syn-cre (B), gntR-credown (C) and xynP-cre (D). All samples and the HBS-EP running buffer were supplemented with 50 µM HPrSer46P, in the case of gntRcredown 75 µM HPrSer46P were used. The sensorgrams were obtained from SPR measurements with samples containing the CcpA(His)6 concentrations listed at the right side of each diagram. Dotted lines represent the real experimental data, bold black lines the calculated 1:1 Langmuir binding fits calculated by BIAEVALUATION, version 4.1. All measurements were reproduced at least three times with two biological replicates. RU: Response Units, Resp. Diff. [RU]: signal difference from FC2 (cre) and an unspecific oligonucleotide coupled to the reference cell FC1. - 67 - Results The resulting kinetic data from the 1:1 Langmuir binding fit with the corresponding χ2-values which indicate the average deviation of the experimental data and the fits were given in Table 5.3. Table 5.3: Kinetic data obtained from SPR measurements validating the interaction of CcpA-HPrSer46P with different cre-elements. ka: association rate constant, kd: dissociation rate constant, KD: dissociation constant, χ²: deviation between experimental data and calculated fits. ackA-cre2 gnt-credown syn-cre xylA-cre xynP-cre ka [M-1s-1] 6.0 ± 1.9 x 105 6.0 ± 1.7 x 105 3.2 ± 0.6 x 105 1.1 ± 0.3 x 106 5.2 ± 1.0 x 106 kd [s-1] 9.5 ± 1.0 x 10-4 1.8 ± 0.3 x 10-3 1.2 ± 0.2 x 10-3 3.6 ± 1.5 x 10-3 1.2 ± 0.2 x 10-3 KD [nM] 1.6 ± 0.4 3.0 ± 0.4 3.8 ± 0.1 3.3 ± 0.5 0.2 ± 0.1 χ2 3.5-4.4 3.4-5.2 5.8 0.5-2.1 2.9-5.8 The kinetics of the interaction of CcpA-HPrSer46P with different cre-elements are very similar except for the interaction with xynP-cre. In this case, the formation of the protein-DNA complex was up to 16.5 times faster and as a consequence, the KD-value was up to 19 times lower (compare to Figure 5.20). Figure 5.20: Comparison of the ka- and KD-values obtained from the interaction analysis of CcpAHPrSer46P with various cre-elements. These graphics visualizes the differences of the association rate constants (A) and the resulting dissociation constants (B) obtained from titrations of CcpA-HPrSer46P to various cre-elements. The standard deviation is represented for ea The highest affinity of CcpA-HPrSer46P to xynP-cre is caused by an up to 16.5-fold faster association of the complex. - 68 - Discussion 6 Discussion 6.1 Indications for the formation of a TCA cycle metabolon The existence of a citrate cycle metabolon, a “supramolecular complex of sequential enzymes and structural elements”, was proposed already in the 1980s (Srere, 1985). Mainly, four distinct methods were used to prove the existence of such a multi-enzyme complex: Analysis of crude lysates of total rat liver mitochondria (Robinson et al., 1987; Robinson & Srere, 1985), theoretical calculations and bioinformatics (Elcock & McCammon, 1996; Lyubarev & Kurganov, 1989; Tompa et al., 1987a; Vélot et al., 1997), in vivo analysis via FRAP, SPINE and bacterial-two-hybrid experiments (Haggie & Verkman, 2002; Meyer et al., 2011) and protein-protein affinity gelelectrophoresis and analytical size exclusion chromatography with purified proteins (Beeckmans & Kanarek, 1981; Beeckmans et al., 1989; Datta et al., 1985; Morgunov & Srere, 1998). The interactions of some particular enzymes have been examined so far, e.g. between malate dehydrogenase and citrate synthase in mitochondria of Saccharomyces cerevisiae or pig heart (Grandier-Vazeille et al., 2001; Lindbladh et al., 1994; Mattlasson et al., 1974; Morgunov & Srere, 1998), pig heart α-ketoglutarate dehydrogenase and succinate thiokinase (Porpaczy et al., 1983) or E. coli aconitase and isocitrate dehydrogenase (Tsuchiya et al., 2008). Moreover, the interaction between mitochondrial malate dehydrogenase and citrate synthase from pig heart has been quantified already (Jameson & Seifried, 1999; Tompa et al., 1987b). However, none of these studies reported an influence of the substrates together with the corresponding cofactors on the interactions of TCA cycle proteins. In this study, the interaction of B. subtilis malate dehydrogenase Mdh and isocitrate dehydrogenase Icd was characterized in vitro via SPR because this technique allows not only the visualization and precise kinetic analyses of protein interactions in real time but also the analysis of protein-protein interactions with additional possible interactants like substrates or cofactors (Liedberg et al., 1995; Mol & Fischer, 2010; Safina, 2012). Initially, the interaction between Mdh and Icd was analyzed without any substrates and cofactors. These data revealed only a very weak interaction of the two enzymes, a complex probably formed in situ nascendi caused by co-expression of the two enzymes as their encoding genes mdh and icd are organized in one operon (Jin et al., 1996; Jin & Sonenshein, 1994b). As this interaction was so weak, an additional stimulation appeared to be reasonable. The influence of the substrates and coeffectors on the formation of a metabolon has already been postulated in literature (McKenna, 2011; Ovádi & Srere, 1996). However, it has never been detected experimentally. Therefore, in this study, the interaction of Icd and Mdh was analyzed in the presence - 69 - Discussion of their substrates and cofactors. Only isocitrate, the educt of Icd, in combination with the corresponding cofactors NADP+ and Mg2+, stimulated the formation of an Mdh-Icd complex. Single metabolites without the corresponding cofactors or combinations of malate and NAD+, oxaloacetate and NADH and α-ketoglutarate and NADPH, did not influence the interaction. This analysis demonstrated the Mdh-Icd complex for the first time in vitro and, additionally, the metabolic triggers of this complex formation. A more detailed view of this interaction was obtained by the kinetic analysis. Kinetic constants were not obtained from the interaction without any supplements, since the interactions were too weak to perform a valid evaluation. The kinetic analysis in the presence of isocitrate, NADP+ and Mg2+ turned out to be difficult as well, because Icd converted isocitrate and NADP+ to α-ketoglutarate and NADPH even during the short time scale of a sensorgram. This conversion was also visible in SPR, as these sensorgrams indicated almost the same bulk effects like sensorgrams obtained from pure α-ketoglutarate/NADPH injections. In contrast to wildtype Icd, measurements with the mutant IcdS104P indicated almost no bulk effect. This is in good agreement with enzymatic activity assays, as this mutant is 174-fold less active in comparison to the wildtype enzyme. This might be due to a reduced flexibility of the active site as serine 104 is exchanged to proline and to an involvement of S104 in substrate binding. In E. coli, the corresponding residue serine 113 is involved in the binding of isocitrate and its orientation towards NADP +. Additionally, it is responsible for the substrate specificity of Icd, e.g. IcdS113E mutants recognize isopropylmalate as substrate. However, there has been no indication so far, that serine 113 is directly involved in catalysis (Dean & Koshland, 1993; Doyle et al., 2001; Doyle et al., 2000; Gonçalves et al., 2012). IcdS104P bound Mdh with a higher affinity than wildtype Icd, so the proline mutation might fix the substrate bound conformation which favors the interaction with Mdh. The quantification of their interaction revealed a high dissociation constant (KD) with 5.0 ± 0.1 µM. The binding of the IcdS104P mutant to Mdh was also stimulated by isocitrate, Mg2+ and NADP+. However, due to the reduced enzymatic activity, the amounts of isocitrate and NADP+ remained almost constant which enabled the kinetic analysis of the stimulated interaction between Mdh and Icd for the first time. The resulting KD value was 150 ± 0.3 nM and was 33.3-fold lower than the non-stimulated interaction. Interestingly, the dissociation rate constant (kd) was 3.8-fold slower if the substrates of Icd were available. This indicates a stabilizing effect of the metabolites on the complex. Isocitrate, NADP+ and Mg2+ stimulate the association of the Mdh-IcdS104P complex and as a consequence, the association rate constant (ka) is 8.5-fold higher in comparison to the interaction without any metabolites. As the interaction between wildtype Icd and Mdh without isocitrate, NADP+ and Mg2+ is weaker, an even higher stimulation effect caused by the substrates is likely. Interestingly, recent studies revealed the kinetics of the interactions between the glycolytic enzymes phosphofructokinase (PfkA) and enolase (Eno) in B. subtilis (Newman et al., 2012). These two enzymes form a supramolecular complex - 70 - Discussion together with some enzymes of the RNA degradasome (Commichau et al., 2009; Lehnik-Habrink et al., 2010; Newman et al., 2011; Newman et al., 2012). In comparison to Mdh-Icd interaction, the complex formation between PfkA and Eno is 10-fold faster even without any stimulation of metabolites. As a consequence, the KD value is 3.8-fold lower with only 40 nM. However, the influences of the metabolites on this interaction have not been investigated yet (Newman et al., 2012). Stimulation on metabolon formation has already been described for the E. coli and Salmonella typhimurium tryptophan synthase. The two enzymes α2 and β2 synthesize L-tryptophan and are connected via a tunnel structure which the intermediates have to pass during the synthesis. Similar to the Mdh-Icd complex, the binding between α2- and β2- subunit is strongly influenced by the intermediates of tryptophan synthesis. Structural analysis revealed a conformational change of the two enzymes, as soon as the starter molecule 3-indole-D-glycerol 3’ phosphate (IGP) is bound to its binding pocket. The enzyme complex enters the closed conformation which allows the synthesis of Ltryptophan. Nearly every intermediate of the biosynthesis causes a different conformation of the complex of the two enzymes (Dunn, 2012; Dunn et al., 1990; Hyde et al., 1988). Another example is the catabolism of branched-chain amino acids (BCAA) in human mitochondria. The branched-chain aminotransferase (hBCATm) did not only interact with the E1-part of the branched-chain α-keto acid dehydrogenase complex (BCKD), moreover, the interaction led to 5.9-fold increase of the kcat value of E1 (Islam et al., 2007). In contrast to all the examples above, the stimulation of the interaction between Mdh and Icd/IcdS104P in the presence of the substrates of Icd was unexpected as the two enzymes do not catalyze subsequent reactions in the TCA cycle. Moreover, the enzymatic activity of Icd doubled, if Mdh was available. In contrast to wildtype Icd, Mdh had no influence on the enzymatic activity of IcdS104P. However, the fixed N-Cα angle of proline might obstruct conformational changes during the formation of α-ketoglutarate and therefore, Mdh does not affect the enzymatic activity of the IcdS104P mutant. In contrast, the enzymatic activity of Mdh is not affected by Icd. This would imply an accumulation of intracellular α-ketoglutarate, if its increased formation would only serve catabolism. Interestingly, the products of both Mdh and Icd serve as precursor molecules for amino acid synthesis: on the one hand, α-ketoglutarate is the precursor molecule for glutamate, glutamine, arginine and proline (Baumberg & Klingel, 1993; Brill et al., 2011; Gunka & Commichau, 2012; O'Reilly & Devine, 1994; Utagawa, 2004). On the other hand, oxaloacetate is needed for aspartate, asparagine, lysine, methionine and threonine biosynthesis (see Figure 6.1) (Belitsky, 2002; Parsot, 1986; Paulus, 1993; Rodionov et al., 2003; Wu et al., 2011). The metabolite α-ketoglutarate serves as linker between carbon and nitrogen metabolism because it provides the carbon backbone of de novo synthesized glutamate and glutamine (Desphande et al., 1980; Sonenshein, 2007). As the presence of Mdh doubles the enzymatic activity of Icd, this - 71 - Discussion interaction might stimulate the de novo synthesis of glutamate which plays a major role in nitrogen metabolism. In B. subtilis, in at least 37 transamination reactions glutamate serves as donor of the amino group (Oh et al., 2007). During the exponential growth phase, flux analysis revealed that half of the intracellular α-ketoglutarate is used for glutamate synthesis and as a consequence the intracellular amounts of glutamate are high with 40-70 mM (Buescher et al., 2012; Rühl et al., 2012). Similar data were obtained from E. coli (Bennett et al., 2009). The formation of the Mdh-Icd complex depends on the availability of isocitrate and NADP+ in addition to Mg2+ as metal ion cofactor of Icd. The intracellular amounts of isocitrate and NADP+ are high as long as favorable carbon sources like glucose are available (Dauner et al., 2001a; Dauner et al., 2001b; Kleijn et al., 2010; Rühl et al., 2012; Tännler et al., 2008). However, in the stationary phase, the analysis of the NADPH/NADP+ ratio of B. megaterium revealed an increase of the intracellular NADPH amount because glucose limitation leads to low NADPH consumption (Rühl et al., 2012; Sauer et al., 1997; Setlow & Setlow, 1977; Tännler et al., 2008). Additionally, in stationary phase under nitrogen starving conditions, flux analysis revealed no metabolic fluxes to serve amino acid anabolism. Likewise, in contrast to exponential growth, the flux through the TCA cycle is reduced 2-3 times in stationary phase (Rühl et al., 2012). In summary, these data suggest, that the interaction of Mdh with Icd and also the increased α-ketoglutarate formation is stimulated by substrate and cofactor of Icd in response to high carbon and NADP+ supply to enhance glutamate synthesis. In contrast to the unexpected interaction between Mdh and Icd, the interaction between Mdh and CitZ was presumed for quite some time because oxidation of malate to oxaloacetate is highly endergonic (Miller & Smith-Magowan, 1990; Ochoa, 1955). The reaction is driven by the fast conversion of oxaloacetate by CitZ and this demands a closed proximity of both enzymes. Surprisingly, in B. subtilis, no interaction between Mdh and the subsequent enzyme CitZ was detectable via SPR. However, this protein-protein interaction has been already proven and quantified in other organisms, e.g. purified citrate synthase of pig heart mitochondria bound to malate dehydrogenase which was covalently coupled to a sepharose 4B column (Beeckmans & Kanarek, 1981; Morgunov & Srere, 1998; Tompa et al., 1987b). Mainly, the interaction of both enzymes was assumed from indirect evidences, e.g. in vitro trapping assays with mitochondrial malate dehydrogenase and citrate synthase from pig hearts revealed no release of oxaloacetate to the medium (Datta et al., 1985; Morgunov & Srere, 1998). - 72 - Discussion Figure 6.1: Schematic overview of the TCA cycle in B. subtilis. The metabolic intermediates are written in black. The enzyme names are highlighted in green, the reducing equivalents in blue color. The B. subtilis specific abbreviations of the enzyme names are marked with black boxes. Red arrows indicate the connections between TCA cycle and amino acid and nucleotide anabolism. The enzymes analyzed in this study, Mdh, Icd and CitZ are highlighted in yellow. The blue arrow represents the interaction between Mdh and Icd. As the direct interaction between Mdh and CitZ was not detectable, a third enzyme might be needed to facilitate complex formation in B. subtilis. In TCA cycle, aconitase (CitB) follows CitZ and it represents one possible interaction partner. Computational models with the mitochondrial malate dehydrogenase, citrate synthase and aconitase of yeast revealed theoretical structures, which allow the interaction of the three enzymes (Vélot et al., 1997). Even more, there have been hints on the existence of a supramolecular complex containing further TCA cycle enzymes. For E. coli and Pseudomonas aeruginosa, size exclusion chromatography experiments with crude lysates revealed always co-elution of citrate synthase and malate dehydrogenase together with several other TCA cycle enzymes (Barnes & Weitzman, 1986; Mitchell et al., 1995). SPINE data revealed in vivo interactions of several TCA cycles enzymes including Mdh and CitZ of B. subtilis as well (Meyer et al., 2011). At the moment, there are two main hypotheses on the conformation of an enzymatic complex: a ring structure with a kind of “substrate assembly line” or a higher organized complex with an anchor enzyme that fixes the whole metabolon (Lyubarev & Kurganov, 1989). The Mdh-Icd - 73 - Discussion complex supports the latter type of supramolecular organization, as these two enzymes do not catalyze any subsequent interactions. Additionally, a ring structure has some disadvantages for the cell: It is very big and a ring always has a reduced stability, as it only allows the interaction of two neighboring enzymes (Figure 6.2 A). Until now, there are more evidences for a compact structure of the TCA metabolon with one fixation of the complex at the cytoplasma membrane. The possible anchor enzyme has been described recently. In B. subtilis the bifunctional succinate dehydrogenase complex SdhABC is attached to the cytoplasmic membrane. It functions as fumarate reductase in the aerobic respiratory chain and as succinate dehydrogenase in the Krebs cycle (García Montes de Oca et al., 2012). In E. coli, the corresponding SdhABCD complex is anchored within the membrane by the SdhCD subunits, while the subunits SdhA and B are oriented towards the cytoplasm. A similar conformation is assumed for the B. subtilis enzyme as well (García Montes de Oca et al., 2012; Schnorpfeil et al., 2001; Sousa et al., 2011; Sousa et al., 2012; Yankovskaya et al., 2003). A formation of a supramolecular enzyme complex around these subunits might be possible. The arrangement of the compact complex might enable more interactions between different enzymes than a defined ring structure. Additionally, around this anchor enzyme, smaller subcomplexes can be formed depending e.g. on the availability of metabolites, e.g. the analyzed Mdh-Icd complex in the presence of the substrates of Icd during exponential growth (Figure 6.2 B). Figure 6.2: Hypothetical models of a B. subtilis TCA metabolon. This scheme indicates two possible TCA metabolon structures. A: the rigid “assembly line ring model” which allows only the interaction of subsequent enzymes. B: the “compact complex model” which allows the interaction between more than just two enzymes. Black arrows with question marks indicate possible, but experimentally unconfirmed interactions. The proven interaction between Mdh and Icd is depicted with a green arrow and an exclamation mark. CM: cytoplasmic membrane; CitB: aconitase, CitG: fumarase, Icd: isocitrate dehydrogenase, Mdh: malate dehydrogenase, OdhAB, PdhD: α-ketoglutarate dehydrogenase complex, SdhABC: succinate dehydrogenase complex, SucCD: succinate thiokinase. - 74 - Discussion 6.2 Structure and kinetics of the CcpA-HPrSer46P complex interacting with various cre-elements CcpA is the central regulator protein in B. subtilis, which controls e.g. carbon catabolite regulation (Fujita, 2009), overflow metabolism (Grundy et al., 1993; Shivers et al., 2006), expression of some TCA cycle enzymes (Blencke et al., 2006; Jin & Sonenshein, 1994b; Tobisch et al., 1999; Wünsche et al., 2012), amino acid anabolism (Ludwig et al., 2002; Shivers & Sonenshein, 2005; Tojo et al., 2005), sporulation (Piggot & Hilbert, 2004) and biofilm formation (Chagneau & Saier Jr, 2004; Stanley et al., 2003). In complex with either HPrSer46P or CrhSer46P as coeffectors, CcpA contacts its operator sequence cre and activates or represses gene expression (Deutscher, 2008; Deutscher et al., 1995; Fujita, 2009; Titgemeyer & Hillen, 2002). In this study, the binding of the CcpA-HPrSer46P complex to five different cre-elements was quantified in vitro via SPR measurements. Interestingly, the KD-values did not differ significantly for four analyzed cre-elements, in particular ackA-cre2, gntR-credown, syncre and xylA-cre with approximately 3.0 nM. The affinity of the complex to ackA-cre2, gntR-credown and syn-cre was analyzed as well by fluorescence polarization and revealed similar dissociation constants (Schumacher et al., 2011). Other transcriptional regulators bind with similar affinities to their operator sites, e.g. AbrB regulates gene expression at the transition from exponential to stationary growth phase, and its affinity to its operator sites ranges from 6-40 nM (Strauch, 1995; Strauch et al., 1989). However, the high similarity of the kinetics was unexpected, as the analyzed cre-elements share only three highly conserved parts, in particular the CG-Pairs at position 2/14 and 8/9 and Thy2 and Ade15 at the 5’ and 3’ end of the cre-element (Miwa & Fujita, 2001; Miwa et al., 2000; Schumacher et al., 2011; Weickert & Chambliss, 1989). Crystal structures of CcpA-HPrSer46P in complex with ackA-cre2, gntR-credown and syn-cre revealed that these conserved regions are recognized by the CcpA-DNA binding domain. The overall structure of all three complexes was highly homologous with the earlier published structure of B. megaterium CcpA (Schumacher et al., 2004) and unveiled only three important universal amino acid-DNA contacts (refer to Figure 6.3). As part of the hinge helix, Leu56 interacts with the central CpG step in the minor groove of the DNA. Helix-turnhelix (HTH) residue Arg22 contacts Gua3 of the major groove (Schumacher et al., 2011). The same contacts were detected for the corresponding residues Leu55 and Arg21 of B. megaterium CcpA and these residues are highly conserved throughout many CcpA proteins from different species, e.g. of B. anthracis, B. halodurans or Listeria monocytogenes (Schumacher et al., 2004). Even more, other proteins of the LacI/GalR family, like LacR, GalR and PurR all share a highly conserved leucine residue in their DNA binding domains (Lewis et al., 1996; Schumacher et al., 2004; Schumacher et al., 1994). The importance of the interaction with the central CpG step is underlined as in all structures the - 75 - Discussion hinge helix backbone atoms exhibited weak but specific hydrogen bonds to the nucleobases of the central CpG step (Schumacher et al., 2011). Figure 6.3: Crystal structure of the HTHLH domains and hinge helices, the two separable DNA-binding modules of CcpA (Schumacher et al., 2011). Arg22 (blue) as part ot the HTHLH motifs (pink ribbons) contacts Gua3 of the cre-site in the major groove of the DNA. The hinge helices (yellow ribbons) as second DNA-binding module interact via Leu56 (blue) with the central CpG step with the minor groove of the DNA. The two modules areconnected via flexible linkers which allow the optimal movement towards the conserved sequences. This figure represents an overlay of the structure of the CcpA-HPrSer46P-ackA-cre2 and the syn-cre-complex as these two crystal structures show the highest deviations. The backbone of the DNA is depicted in grey, the residues which have van der Waals contacts with the cre-element are presented with blue dotted surfaces. Besides the two CG-pairs, the borders of the cre-element, Thy2 and Ade15, are highly conserved among the cre-elements analyzed so far (Miwa et al., 2000; Weickert & Chambliss, 1990). The Cβ atom of residue Asn29 from each subunits contacts Thy2. This interaction was exclusively detected for the B. subtilis CcpA-HPrSer46P-cre structures (Schumacher et al., 2011). So the interaction between the CcpA-HPrSer46P-complex and the cre-sites mainly depends on three nucleobase-amino acid interactions. This might explain why these highly degenerate sequences were bound with almost the same affinity. However, besides the universal interactions between CcpA residues and nucleotides of the cre-site, there are some cre-specific contacts as well, e.g. the interaction between Thy11 of gntR-credown and Met17 of each CcpA half site. As in vivo analysis with CcpAM17R mutants revealed a complete loss of repression of gntR expression, this represents a typical example of differential regulation (Sprehe et al., 2007). - 76 - Discussion Besides the same kinetics and the same overall structures of the analyzed CcpA-HPrSer46P complexes, there was one significant difference between all structures. The bending angle of the creelements varied. CcpA is able to adjust the DNA conformation by the flexible linkers between the helix-turn-helix-loop-helix (HTHLH) motif and the hinge helices. This leads to different bends of the whole cre-site: syn-cre is bent by 31°, ackA-cre2 56° and gntR-credown by 41° (Schumacher et al., 2011). The high plasticity of the molecule enables the recognition of the degenerate cre-sites with the same affinity. This DNA kinking is typical for regulator proteins and was extensively studied for the E. coli cAMP receptor protein CRP which is the main regulator of CCR in Gram-negative bacteria. It activates transcription of more than 100 genes and thereby, it is able to bend its operator DNA by approximately 80° (Busby & Ebright, 1999; Chen et al., 2001; de Crombrugghe et al., 1984; Lawson et al., 2004). In contrast to the cre-sites, the eleven base pairs of the CAP regions are higher conserved and show strong sequence preferences at position 1, 2 and 4-8 of each half-site (refer to Table 6.1) (Dalma-Weiszhausz et al., 1991; Ebright et al., 1989; Gartenberg & Crothers, 1988; Gunasekera et al., 1992). Like CcpA, CRP forms also a dimer which recognizes its perfect palindromic CAP-site (Parkinson et al., 1996). The activated CcpA-complex contacts only 6 of 16 nucleotides of the cre-site, while CRP interacts specifically with 14 of 22 of the CAP-site. This might be one reason, that the affinity of CRP for the CAP binding site is 210-fold higher than of the CcpA-HPrSer46P complex to cre (Gunasekera et al., 1992). Table 6.1: Comparison of the consensus sequences of the E. coli CAP binding site and the B. subtilis cre-site. Specifically bound nucleotides were highlighted in bold red letters Sequence: 5‘ → 3‘ CAP binding A A A T G T G A T C T A G A T C A C A T T T site (E. coli) creconsensus N T G W N A N C G N T N W C A N sequence (B. subtilis) Reference (Ebright et al., 1989) (Weickert & Chambliss, 1990) Similar to the CAP-site, the xynP-cre-site is besides one exception completely palindromic. In contrast to the other analyzed cre-elements, the affinity of the CcpA-HPrSer46P complex to xynP-cre was up to 19-fold higher with a KD value of 0.2 ± 0.1 nM. It was already stated that the functionality of a creelement depends on its palindromic nature (compare to Table 6.2), so this might be a reason that the affinity of CcpA-HPrSer46P to xynP-cre is highest among all cre-sites analyzed in this study (Fujita, - 77 - Discussion 2009; Galinier et al., 1999; Marciniak et al., 2012). However, the xylA-cre of B. megaterium is bound with a similar affinity of 0.6 ± 0.3 nM and in contrast to xynP-cre this cre-element has only 8 of 16 palindromic basepairs (Rygus & Hillen, 1992; Seidel et al., 2005). Table 6.2: Palindromic nature of the B. subtilis xynP- and B. megaterium xylA-cre-sequence. Palindromic bases are highlighted in the same color, non-palindromic parts of the cre-elements are written in black. xynP-cre (B. subtilis) xylA-cre (B. megaterium) Sequence: 5‘ → 3‘ Reference TGAAAGCGCTTTTA (Galinier et al., 1999) TGAAAGCGCAAACA (Gösseringer et al., 1997) In vivo analysis of the promoter strength of several CcpA regulated genes via lacZ-measurements revealed an up to 44-fold higher repression of the expression of the xynP promoter in contrast to the gntR promoter (Sprehe et al., 2007). As the affinity of the CcpA-HPrSer46P-complex to xynP-cre is 15fold higher in comparison to gntR-cre, this likely represents the reason for the better regulation. In addition, the distance between cre-element and transcription start also influences the regulation by CcpA: in the case of xynP, there is one full helix turn between transcription start and cre-element. If the cre-element is located in close vicinity to the transcription start, the repression factor was lower, e.g. in the case of gntR (Marciniak et al., 2012). However, although the affinity of the CcpAHPrSer46P complex to ackA-cre2 is 8-fold reduced in comparison to xynP-cre, the substitution of ackA-cre2 by xynP-cre and vice versa did not improve or impair the regulation of xynP and ackA expression (Sprehe, 2007). Additionally, fluorescence titrations of xylA-cre, xynP-cre, gntR-credown and ackA-cre2 to a CcpA1W mutant and HPrSer46P revealed no significant differences of the affinity to the analyzed cre-sites (Diel, 2005; Seidel et al., 2005). However, as until now the CcpA-HPrSer46PxynP-cre crystall structure has not been determined yet, a structural reason for this better affinity remains unclear. In summary, CcpA-HPrSer46P recognizes highly degenerated cre-elements with almost the same affinity. This is achieved by the high plasticity of the protein due to the flexibility of the linker regions between the two DNA binding domains, the HTHLH motifs and the hinge helices. As a consequence, the global regulator CcpA binds universally to all analyzed cre-sites with Arg22 and Leu56 and is able to interact with multiple sequences in the same way. - 78 - Discussion 6.3 Outlook The determination of the conditions and kinetics of the Mdh-Icd interaction was the first step to understand the formation of the TCA cycle metabolon. The next steps will be the purification of the other TCA cycle enzymes and the analysis of their interaction in combination with the corresponding metabolites via SPR. 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EtOH F-6-P FBP Fig. g Glc-6-P HEPES HPr HPrSer46P HTHLH Icd IcdS104P IPTG ka Kam KD kd LB Mdh Mg NAD(P)H % (volume/volume) % (weight/volume) Ammonium peroxodisulfate Adenosine triphosphate Absorption at λ=x nm Bacillus base pairs bovine serum albumin Chloramphenicol cyclic Adenosinemonophosphate Catabolite activator protein Catabolite control protein A carbon catabolite regulation Aconitase Citrate synthase cAMP receptor protein Desoxyribonucleic acid Desoxyribonuclease I dithiothreitol Escherichia Ethylendiamin tetraacetate „et alii“ (and others) Ethanol fructose-6-phosphate fructose-1,6-bisphosphate figure gravity (9.81 m/s2) glucose-6-phosphate 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid Histidin containing protein histidine containg protein phosphorylated at serine 46 residue Helix-turn-helix-loop-helix Isocitrate dehydrogenase Isocitrate dehydrogenase mutant with serine 104 exchange to proline Isopropyl β-D thiogalactopyranoside Association rate constant Kanamycin Dissociation constant Dissociation rate constant Luria-Bertani (broth) Malate dehydrogenase Magnesium β Nicotinamide adenine dinucleotide hydrate (2-phosphate) - 102 - Abbreviation index NTA ODx P p. a. PAA PAGE PCR PEP PMSF PTS R RNase A rpm RT RU SDS SPR TAE TBE TCA cycle TE TEMED Tris Tris v w wt β-ME Nitrilotetraacidic acid Optical density at λ=x nm phosphate „pro analysi“ polyacrylamide polyacrylamide gel electrophoresis polymerase chain reaction phosphoenolpyruvate Phenylmethylsulfonylfluorid phosphoenolpyruvate:sugar phosphotransferase system resistance ribonuclease A revolutions per minute room temperature reponse units sodiumdocecylsulfate Surface plasmon resonance Tris-acetate-EDTA buffer Tris-borate-EDTA buffer tricarboxylic acid cycle Tris-EDTA N,N,N’,N’-Tetramethylethylendiamine Tris-(hydroxymehtyl)-aminomethan 2-Amino-Hydroxymethylpropan-1,3-diol Volume weight wild-type β-mercaptoethanol Units ‘ °C Da g h l M m min s T t U V W minute Degree Celsius Dalton (g/mol) gram hour liter mol/liter meter minute second temperature time Units of enzymatic activity volt watt - 103 - Abbreviation index Dimensions 3 k kilo (10 ) m milli (10 ) μ micro (10 ) n nano (10 ) p pico (10 ) f femto (10 ) -3 -6 -9 -12 -15 Nucleotides A C G T N R S Y W Adenosine Cytidine Guanosine Thymidine A, C, G, T G, A C, G C, T A, T Amino acid nomenclature (according to IUPAC, 1969) A C D E F G H I K L M N P Q R S T V W Y Ala Cys Asp Glu Phe Gly His Ile Lys Leu Met Asn Pro Gln Arg Ser Thr Val Trp Tyr Alanine Cysteine Aspartate Glutamate Phenylalanine glycine Histidine Isoleucine Lysine Leucine Methionine Asparagine Proline Glutamine Arginine Serine Threonine Valine Tryptophan Tyrosine - 104 - - 105 - Publications Publications Schumacher, M. A., Sprehe, M., Bartholomae, M., Hillen, W., & Brennan, R. G. (2011). Structures of carbon catabolite protein A-(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators. Nucleic Acids Res., 39(7), 2931-2942. Wünsche, A., Hammer, E., Bartholomae, M., Völker, U., Burkovski, A., Seidel, G., & Hillen, W. (2012). CcpA forms complexes with CodY and RpoA in Bacillus subtilis. FEBS J, 279(12), 2201-2214. - 106 - Versicherung an Eides statt Versicherung an Eides statt Hiermit erkläre ich an Eides statt, dass ich diese Arbeit selbstständig verfasst und keine anderen als die von mir angegebenen Quellen und Hilfsmittel verwendet habe. Erlangen, den 04.02.2013 - 107 -